Restriction map for
pFN21AE6717
(Circular) (Six-base) MAPSORT of: pFN21AE6717.seq Check: 9450 from: 1 to: 6919
pFN21AE6717 6919 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2669 2676 4729 278
Size: 53 83 186 936 1133 7 2053 2468
Fragments arranged by size:
2468 2053 1133 936 186 83 53 7
AccI GT'mk_AC
Cuts at: 1937 4262 1937
Size: 2325 4594
Acc65I G'GTAC_C
Cuts at: 4240 4240
Size: 6919
AclI AA'CG_TT
Cuts at: 1563 5046 5419 1563
Size: 3483 373 3063
Fragments arranged by size:
3483 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2645 2804 3501 3557 3842 3860 4982 6366
Size: 1013 159 697 56 285 18 1122 1384
Cuts at: 6366 6634 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1122 1013 697 285 268 159
56 18
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6722
AflIII A'CryG_T
Cuts at: 1203 5818 6795 1203
Size: 4615 977 1327
Fragments arranged by size:
4615 1327 977
AgeI A'CCGG_T
Cuts at: 5750 5750
Size: 6919
AhdI GACnn_n'nnGTC
Cuts at: 5648 5648
Size: 6919
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6919
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2588 1184
Size: 1404 5515
AlwNI CAG_nnn'CTG
Cuts at: 2635 4662 6234 2635
Size: 2027 1572 3320
Fragments arranged by size:
3320 2027 1572
ApaLI G'TGCA_C
Cuts at: 4976 6132 4976
Size: 1156 5763
ApoI r'AATT_y
Cuts at: 1493 2331 2508 2531 2944 3637 4228 4454 4606
Size: 838 177 23 413 693 591 226 152
Cuts at: 4606 6715 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 838 693 591 413 226 177
152 23
AseI AT'TA_AT
Cuts at: 160 1032 5473 160
Size: 872 4441 1606
Fragments arranged by size:
4441 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6919
AvaI C'yCGr_G
Cuts at: 1870 1943 4244 1870
Size: 73 2301 4545
Fragments arranged by size:
4545 2301 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4112 4145 4112
Size: 33 6886
BamHI G'GATC_C
Cuts at: 3188 4249 4616 3188
Size: 1061 367 5491
Fragments arranged by size:
5491 1061 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2891 4240 5695 618
Size: 325 217 627 1104 1349 1455 1842
Fragments arranged by size:
1842 1455 1349 1104 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2157 3112 3680 4238 1391
Size: 766 955 568 558 4072
Fragments arranged by size:
4072 955 766 568 558
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3489 928
Size: 971 1590 4358
Fragments arranged by size:
4358 1590 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3523 3557 5110 5144 1903
Size: 34 1586 34 1553 34 3678
Fragments arranged by size:
3678 1586 1553 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4811 6021 4811
Size: 1210 5709
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6919
BfrBI ATG'CAT
Cuts at: 3020 3020
Size: 6919
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2035 5530 136
Size: 107 122 71 631 605 363 3495 1525
Fragments arranged by size:
3495 1525 631 605 363 122 107 71
BglII A'GATC_T
Cuts at: 6914 6914
Size: 6919
BlpI GC'TnA_GC
Cuts at: 4336 4336
Size: 6919
Bme1580I G_kGCm'C
Cuts at: 1790 3699 4980 6136 1790
Size: 1909 1281 1156 2573
Fragments arranged by size:
2573 1909 1281 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6919
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4041 4650 5608
Size: 416 53 83 399 24 2624 609 958
Cuts at: 5608 442
Size: 1753
Fragments arranged by size:
2624 1753 958 609 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6919
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3360 3360
Size: 6919
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2171 2864 2884 3683 3785 5579 1239
Size: 932 693 20 799 102 1794 2579
Fragments arranged by size:
2579 1794 932 799 693 102 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3724 5044 5909 6207 3724
Size: 1320 865 298 4436
Fragments arranged by size:
4436 1320 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2648 5582 882
Size: 617 1149 2934 2219
Fragments arranged by size:
2934 2219 1149 617
BsaAI yAC'GTr
Cuts at: 493 1204 4710 493
Size: 711 3506 2702
Fragments arranged by size:
3506 2702 711
BsaBI GATnn'nnATC
Cuts at: 2091 6913 2091
Size: 4822 2097
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2666 2673 4726 5108
Size: 53 83 186 936 1133 7 2053 382
Cuts at: 5108 5743 275
Size: 635 1451
Fragments arranged by size:
2053 1451 1133 936 635 382 186 83
53 7
BsaWI w'CCGG_w
Cuts at: 5352 5750 6024 6171 6582 5352
Size: 398 274 147 411 5689
Fragments arranged by size:
5689 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6694
Fragments arranged by size:
6694 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6919
BseYI C'CCAG_C
Cuts at: 3229 6122 6618 3229
Size: 2893 496 3530
Fragments arranged by size:
3530 2893 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2457 1163
Size: 573 721 5625
Fragments arranged by size:
5625 721 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4369 5130 6158 1540
Size: 275 186 2368 761 1028 2301
Fragments arranged by size:
2368 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2157 3680 3838 4980 5065 6136 2157
Size: 1523 158 1142 85 1071 2940
Fragments arranged by size:
2940 1523 1142 1071 158 85
BsmI GAATG_Cn'
Cuts at: 4430 4523 4430
Size: 93 6826
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2157 3112 3680 3699 3838 4238 4980
Size: 399 367 955 568 19 139 400 742
Cuts at: 4980 5065 6136 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 955 742 568 400 399 367
139 85 19
BspEI T'CCGG_A
Cuts at: 6582 6582
Size: 6919
BspHI T'CATG_A
Cuts at: 2070 3025 4808 6538 2070
Size: 955 1783 1730 2451
Fragments arranged by size:
2451 1783 1730 955
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2034 4274 844
Size: 1190 2240 3489
Fragments arranged by size:
3489 2240 1190
BsrBI CCG'CTC
Cuts at: 1394 1823 3864 4806 1394
Size: 429 2041 942 3507
Fragments arranged by size:
3507 2041 942 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2860 5414 5588 65
Size: 1183 1612 2554 174 1396
Fragments arranged by size:
2554 1612 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5563 5750 1700
Size: 110 3753 187 2869
Fragments arranged by size:
3753 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6919
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6919
BssSI C'ACGA_G
Cuts at: 3542 4979 5991 3542
Size: 1437 1012 4470
Fragments arranged by size:
4470 1437 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2695 794
Size: 1901 5018
BstBI TT'CG_AA
Cuts at: 5723 5723
Size: 6919
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6919
BstXI CCAn_nnnn'nTGG
Cuts at: 3046 3494 3046
Size: 448 6471
BstYI r'GATC_y
Cuts at: 1972 3188 4249 4616 5001 5018 6470 6556 6568
Size: 1216 1061 367 385 17 1452 86 12
Cuts at: 6568 6914 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1216 1061 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3281 3281
Size: 6919
BtgI C'CryG_G
Cuts at: 513 1065 2004 2181 513
Size: 552 939 177 5251
Fragments arranged by size:
5251 939 552 177
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3311 487
Size: 683 56 2085 4095
Fragments arranged by size:
4095 2085 683 56
BtsI GCAGTG_nn'
Cuts at: 2689 3080 3908 4435 5229 5249 2689
Size: 391 828 527 794 20 4359
Fragments arranged by size:
4359 828 794 527 391 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6919
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3498 3533 529
Size: 35 2934 35 3915
Fragments arranged by size:
3915 2934 35 35
DraI TTT'AAA
Cuts at: 4223 4223
Size: 6919
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5926 809
Size: 507 4610 1802
Fragments arranged by size:
4610 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2746 2954 4366
Size: 54 1425 50 275 18 916 208 1412
Cuts at: 4366 5255 6884 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1412 916 889 275 208 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4366 1537
Size: 275 2554 4090
Fragments arranged by size:
4090 2554 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2289 3981 4849 1353
Size: 936 1692 868 3423
Fragments arranged by size:
3423 1692 936 868
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5506 5880 6026 387
Size: 1064 138 3917 374 146 1280
Fragments arranged by size:
3917 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2155 3678 2155
Size: 1523 5396
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2171 2645 2804 2864 2884 3501 3557
Size: 393 539 474 159 60 20 617 56
Cuts at: 3557 3683 3785 3842 3860 4982 5579 6366 6634
Size: 126 102 57 18 1122 597 787 268
Cuts at: 6634 1239
Size: 1524
Fragments arranged by size:
1524 1122 787 617 597 539 474 393
268 159 126 102 60 57 56 20
18
EcoNI CCTnn'n_nnAGG
Cuts at: 2030 2030
Size: 6919
EcoO109I rG'GnC_Cy
Cuts at: 3802 3802
Size: 6919
EcoRI G'AATT_C
Cuts at: 2331 2944 4228 4606 2331
Size: 613 1284 378 4644
Fragments arranged by size:
4644 1284 613 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3163 3221 3516 3753 3163
Size: 58 295 237 6329
Fragments arranged by size:
6329 295 237 58
FspI TGC'GCA
Cuts at: 5425 5425
Size: 6919
HaeII r_GCGC'y
Cuts at: 1678 3762 6066 1678
Size: 2084 2304 2531
Fragments arranged by size:
2531 2304 2084
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2304 2336 2880 2912 5560
Size: 32 135 32 732 32 544 32 2648
Cuts at: 5560 5592 5634 5666 1373
Size: 32 42 32 2626
Fragments arranged by size:
2648 2626 732 544 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4263 4509 669
Size: 988 281 2325 246 3079
Fragments arranged by size:
3079 2325 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3569 4156 748
Size: 2821 587 3511
Fragments arranged by size:
3511 2821 587
HpaI GTT'AAC
Cuts at: 4509 4509
Size: 6919
KpnI G_GTAC'C
Cuts at: 4244 4244
Size: 6919
MfeI C'AATT_G
Cuts at: 4518 4518
Size: 6919
MluI A'CGCG_T
Cuts at: 6795 6795
Size: 6919
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6033 6217 1305
Size: 4728 184 2007
Fragments arranged by size:
4728 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2748 2956 10
Size: 54 1425 343 916 208 3973
Fragments arranged by size:
3973 1425 916 343 208 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2811 3044 4877 5236 5395 518
Size: 648 1645 233 1833 359 159 2042
Fragments arranged by size:
2042 1833 1645 648 359 233 159
MspA1I CmG'CkG
Cuts at: 1606 2128 3233 4335 5012 6160 6405 1606
Size: 522 1105 1102 677 1148 245 2120
Fragments arranged by size:
2120 1148 1105 1102 677 522 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2181 513
Size: 552 939 177 5251
Fragments arranged by size:
5251 939 552 177
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6919
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6919
NotI GC'GGCC_GC
Cuts at: 4366 4366
Size: 6919
NsiI A_TGCA'T
Cuts at: 3022 3022
Size: 6919
NspI r_CATG'y
Cuts at: 1132 1229 2575 4277 5822 1132
Size: 97 1346 1702 1545 2229
Fragments arranged by size:
2229 1702 1545 1346 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6919
PciI A'CATG_T
Cuts at: 5818 5818
Size: 6919
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6919
PfoI T'CCnGG_A
Cuts at: 2841 2923 3216 3720 2841
Size: 82 293 504 6040
Fragments arranged by size:
6040 504 293 82
PmeI GTTT'AAAC
Cuts at: 4223 4223
Size: 6919
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2283 2315 4988 5020 6501 6533 2283
Size: 32 2673 32 1481 32 2669
Fragments arranged by size:
2673 2669 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3802 3802
Size: 6919
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6919
PsiI TTA'TAA
Cuts at: 4489 4489
Size: 6919
PstI C_TGCA'G
Cuts at: 830 3351 4086 4271 830
Size: 2521 735 185 3478
Fragments arranged by size:
3478 2521 735 185
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6919
PvuII CAG'CTG
Cuts at: 2128 3233 2128
Size: 1105 5814
SacI G_AGCT'C
Cuts at: 2157 3680 2157
Size: 1523 5396
SalI G'TCGA_C
Cuts at: 1936 4261 1936
Size: 2325 4594
SbfI CC_TGCA'GG
Cuts at: 4271 4271
Size: 6919
ScaI AGT'ACT
Cuts at: 2754 3941 4106 5167 6879 2754
Size: 1187 165 1061 1712 2794
Fragments arranged by size:
2794 1712 1187 1061 165
SfcI C'TryA_G
Cuts at: 826 1045 2110 3347 4082 4267 5402 6083 6274
Size: 219 1065 1237 735 185 1135 681 191
Cuts at: 6274 826
Size: 1471
Fragments arranged by size:
1471 1237 1135 1065 735 681 219 191
185
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6919
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6919
SmaI CCC'GGG
Cuts at: 1872 4246 1872
Size: 2374 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3703 5023 5924 6186 820
Size: 197 926 1760 1320 901 262 1553
Fragments arranged by size:
1760 1553 1320 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4710 493
Size: 4217 2702
SpeI A'CTAG_T
Cuts at: 152 2755 152
Size: 2603 4316
SphI G_CATG'C
Cuts at: 1132 2575 4277 1132
Size: 1443 1702 3774
Fragments arranged by size:
3774 1702 1443
SspI AAT'ATT
Cuts at: 5 52 4843 5
Size: 47 4791 2081
Fragments arranged by size:
4791 2081 47
StuI AGG'CCT
Cuts at: 1517 2658 2738 1517
Size: 1141 80 5698
Fragments arranged by size:
5698 1141 80
StyI C'CwwG_G
Cuts at: 513 1065 2004 2031 2181 3417 3469 3487 4191
Size: 552 939 27 150 1236 52 18 704
Cuts at: 4191 4358 513
Size: 167 3074
Fragments arranged by size:
3074 1236 939 704 552 167 150 52
27 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2406 3482 4031 4946 4963 5116 5301
Size: 1255 826 1076 549 915 17 153 185
Cuts at: 5301 5746 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1076 915 826 549 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2752 2965 3939
Size: 274 80 33 51 1443 775 213 974
Cuts at: 3939 4104 5165 6877 96
Size: 165 1061 1712 138
Fragments arranged by size:
1712 1443 1061 974 775 274 213 165
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3059 3091 6879 6911 3059
Size: 32 3788 32 3067
Fragments arranged by size:
3788 3067 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6919
XbaI T'CTAG_A
Cuts at: 4255 4255
Size: 6919
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6919
XmaI C'CCGG_G
Cuts at: 1870 4244 1870
Size: 2374 4545
XmnI GAAnn'nnTTC
Cuts at: 2298 2331 2658 5048 2298
Size: 33 327 2390 4169
Fragments arranged by size:
4169 2390 327 33
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2667 2674 4727 276
Size: 53 83 186 936 1133 7 2053 2468
Fragments arranged by size:
2468 2053 1133 936 186 83 53 7
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI