Restriction map for
pFN21AE6716
(Circular) (Six-base) MAPSORT of: pFN21AE6716.seq Check: 7360 from: 1 to: 6970
pFN21AE6716 6970 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4780 278
Size: 53 83 186 936 3244 2468
Fragments arranged by size:
3244 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4313 1937
Size: 2376 4594
Acc65I G'GTAC_C
Cuts at: 4291 4291
Size: 6970
AclI AA'CG_TT
Cuts at: 1563 5097 5470 1563
Size: 3534 373 3063
Fragments arranged by size:
3534 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2740 4067 4071 5033 6417 6685 1632
Size: 1108 1327 4 962 1384 268 1917
Fragments arranged by size:
1917 1384 1327 1108 962 268 4
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6773
AflIII A'CryG_T
Cuts at: 1203 5869 6846 1203
Size: 4666 977 1327
Fragments arranged by size:
4666 1327 977
AgeI A'CCGG_T
Cuts at: 5801 5801
Size: 6970
AhdI GACnn_n'nnGTC
Cuts at: 3195 5699 3195
Size: 2504 4466
AleI CACnn'nnGTG
Cuts at: 1166 2232 2552 1166
Size: 1066 320 5584
Fragments arranged by size:
5584 1066 320
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6970
AlwNI CAG_nnn'CTG
Cuts at: 2426 3173 3251 4140 4713 6285 2426
Size: 747 78 889 573 1572 3111
Fragments arranged by size:
3111 1572 889 747 573 78
ApaLI G'TGCA_C
Cuts at: 5027 6183 5027
Size: 1156 5814
ApoI r'AATT_y
Cuts at: 1493 2022 2377 4076 4279 4505 4657 6766 1493
Size: 529 355 1699 203 226 152 2109 1697
Fragments arranged by size:
2109 1699 1697 529 355 226 203 152
AseI AT'TA_AT
Cuts at: 160 1032 3768 5524 160
Size: 872 2736 1756 1606
Fragments arranged by size:
2736 1756 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6970
AvaI C'yCGr_G
Cuts at: 1870 1943 4218 4295 1870
Size: 73 2275 77 4545
Fragments arranged by size:
4545 2275 77 73
AvrII C'CTAG_G
Cuts at: 2474 2474
Size: 6970
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3252 3285 3670 3703 3252
Size: 33 385 33 6519
Fragments arranged by size:
6519 385 33 33
BamHI G'GATC_C
Cuts at: 4300 4667 4300
Size: 367 6603
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4291 5746 618
Size: 325 217 627 2504 1455 1842
Fragments arranged by size:
2504 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4289 1391
Size: 2898 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3615 3902 4215 928
Size: 971 1716 287 313 3683
Fragments arranged by size:
3683 1716 971 313 287
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4156 4190 5161 5195 1903
Size: 34 2219 34 971 34 3678
Fragments arranged by size:
3678 2219 971 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4862 6072 4862
Size: 1210 5760
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6970
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5581 136
Size: 107 122 71 631 605 3909 1525
Fragments arranged by size:
3909 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6965 6965
Size: 6970
BlpI GC'TnA_GC
Cuts at: 2130 4387 2130
Size: 2257 4713
Bme1580I G_kGCm'C
Cuts at: 1790 5031 6187 1790
Size: 3241 1156 2573
Fragments arranged by size:
3241 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6970
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2082 2829 4701
Size: 416 53 83 399 24 665 747 1872
Cuts at: 4701 5659 442
Size: 958 1753
Fragments arranged by size:
1872 1753 958 747 665 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6970
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3273 3425 3465 4142 5630 1239
Size: 2034 152 40 677 1488 2579
Fragments arranged by size:
2579 2034 1488 677 152 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3332 5095 5960 6258 3332
Size: 1763 865 298 4044
Fragments arranged by size:
4044 1763 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2828 3951 5633 882
Size: 617 1329 1123 1682 2219
Fragments arranged by size:
2219 1682 1329 1123 617
BsaAI yAC'GTr
Cuts at: 493 1204 4761 493
Size: 711 3557 2702
Fragments arranged by size:
3557 2702 711
BsaBI GATnn'nnATC
Cuts at: 6964 6964
Size: 6970
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4777 5159 5794 275
Size: 53 83 186 936 3244 382 635 1451
Fragments arranged by size:
3244 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5403 5801 6075 6222 6633 5403
Size: 398 274 147 411 5740
Fragments arranged by size:
5740 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4137 4167 1178
Size: 30 165 30 2734 30 3981
Fragments arranged by size:
3981 2734 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2314 2679 3347 1186
Size: 1128 365 668 4809
Fragments arranged by size:
4809 1128 668 365
BseYI C'CCAG_C
Cuts at: 2134 2748 2793 2802 6173 6669 2134
Size: 614 45 9 3371 496 2435
Fragments arranged by size:
3371 2435 614 496 45 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3442 1163
Size: 573 1706 4691
Fragments arranged by size:
4691 1706 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4420 5181 6209 1540
Size: 275 186 2419 761 1028 2301
Fragments arranged by size:
2419 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2619 4158 5031 5116 6187 2619
Size: 1539 873 85 1071 3402
Fragments arranged by size:
3402 1539 1071 873 85
BsmI GAATG_Cn'
Cuts at: 2118 3032 3357 4002 4481 4574 2118
Size: 914 325 645 479 93 4514
Fragments arranged by size:
4514 914 645 479 325 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2619 4158 4289 5031 5116 6187 1391
Size: 399 829 1539 131 742 85 1071 2174
Fragments arranged by size:
2174 1539 1071 829 742 399 131 85
BspEI T'CCGG_A
Cuts at: 6633 6633
Size: 6970
BspHI T'CATG_A
Cuts at: 3121 4859 6589 3121
Size: 1738 1730 3502
Fragments arranged by size:
3502 1738 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4325 844
Size: 3481 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4857 1394
Size: 429 3034 3507
Fragments arranged by size:
3507 3034 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5465 5639 65
Size: 1183 4217 174 1396
Fragments arranged by size:
4217 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4257 5614 5801 1700
Size: 110 2447 1357 187 2869
Fragments arranged by size:
2869 2447 1357 187 110
BsrGI T'GTAC_A
Cuts at: 96 3107 96
Size: 3011 3959
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6970
BssSI C'ACGA_G
Cuts at: 5030 6042 5030
Size: 1012 5958
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2775 794
Size: 1981 4989
BstBI TT'CG_AA
Cuts at: 5774 5774
Size: 6970
BstEII G'GTnAC_C
Cuts at: 1184 2557 3847 1184
Size: 1373 1290 4307
Fragments arranged by size:
4307 1373 1290
BstXI CCAn_nnnn'nTGG
Cuts at: 2151 2627 3050 2151
Size: 476 423 6071
Fragments arranged by size:
6071 476 423
BstYI r'GATC_y
Cuts at: 1972 2744 3341 4300 4667 5052 5069 6521 6607
Size: 772 597 959 367 385 17 1452 86
Cuts at: 6607 6619 6965 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 959 772 597 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6418
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6231
Fragments arranged by size:
6231 683 56
BtsI GCAGTG_nn'
Cuts at: 2947 3061 3430 4486 5280 5300 2947
Size: 114 369 1056 794 20 4617
Fragments arranged by size:
4617 1056 794 369 114 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6970
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6935
DraI TTT'AAA
Cuts at: 3901 4274 3901
Size: 373 6597
DraIII CAC_nnn'GTG
Cuts at: 3566 3566
Size: 6970
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4145 5977 809
Size: 507 2829 1832 1802
Fragments arranged by size:
2829 1832 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4417 5306 6935
Size: 54 1425 50 275 18 2587 889 1629
Cuts at: 6935 8
Size: 43
Fragments arranged by size:
2587 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4417 1537
Size: 275 2605 4090
Fragments arranged by size:
4090 2605 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2199 4223 4900 1353
Size: 846 2024 677 3423
Fragments arranged by size:
3423 2024 846 677
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5557 5931 6077 387
Size: 1064 138 3968 374 146 1280
Fragments arranged by size:
3968 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2740 3273 3425 3465 4067 4071 4142
Size: 393 1108 533 152 40 602 4 71
Cuts at: 4142 5033 5630 6417 6685 1239
Size: 891 597 787 268 1524
Fragments arranged by size:
1524 1108 891 787 602 597 533 393
268 152 71 40 4
EcoNI CCTnn'n_nnAGG
Cuts at: 3527 3527
Size: 6970
EcoO109I rG'GnC_Cy
Cuts at: 2633 2633
Size: 6970
EcoRI G'AATT_C
Cuts at: 2377 4279 4657 2377
Size: 1902 378 4690
Fragments arranged by size:
4690 1902 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3596 3596
Size: 6970
FspI TGC'GCA
Cuts at: 5476 5476
Size: 6970
HaeII r_GCGC'y
Cuts at: 1678 6117 1678
Size: 4439 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2420 2452 2515 2547 2696
Size: 32 135 32 848 32 63 32 149
Cuts at: 2696 2728 2950 2982 3065 3097 3181 3213 3326
Size: 32 222 32 83 32 84 32 113
Cuts at: 3326 3358 5611 5643 5685 5717 1373
Size: 32 2253 32 42 32 2626
Fragments arranged by size:
2626 2253 848 222 149 135 113 84
83 63 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4314 4560 669
Size: 988 281 2376 246 3079
Fragments arranged by size:
3079 2376 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6970
HpaI GTT'AAC
Cuts at: 4560 4560
Size: 6970
KpnI G_GTAC'C
Cuts at: 4295 4295
Size: 6970
MfeI C'AATT_G
Cuts at: 4569 4569
Size: 6970
MluI A'CGCG_T
Cuts at: 6846 6846
Size: 6970
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2680 6084 6268 1305
Size: 1375 3404 184 2007
Fragments arranged by size:
3404 2007 1375 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5148
Fragments arranged by size:
5148 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2232 2552 2625 3770 3956 4928 5287
Size: 648 1066 320 73 1145 186 972 359
Cuts at: 5287 5446 518
Size: 159 2042
Fragments arranged by size:
2042 1145 1066 972 648 359 320 186
159 73
MspA1I CmG'CkG
Cuts at: 1606 3499 3844 4386 5063 6211 6456 1606
Size: 1893 345 542 677 1148 245 2120
Fragments arranged by size:
2120 1893 1148 677 542 345 245
NaeI GCC'GGC
Cuts at: 4259 4259
Size: 6970
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6418
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6970
NgoMIV G'CCGG_C
Cuts at: 4257 4257
Size: 6970
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6970
NotI GC'GGCC_GC
Cuts at: 4417 4417
Size: 6970
NspI r_CATG'y
Cuts at: 1132 1229 3709 4216 4328 5873 1132
Size: 97 2480 507 112 1545 2229
Fragments arranged by size:
2480 2229 1545 507 112 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6970
PciI A'CATG_T
Cuts at: 5869 5869
Size: 6970
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6970
PmeI GTTT'AAAC
Cuts at: 4274 4274
Size: 6970
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5039 5071 6552 6584 5039
Size: 32 1481 32 5425
Fragments arranged by size:
5425 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2633 2633
Size: 6970
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6970
PsiI TTA'TAA
Cuts at: 4540 4540
Size: 6970
PstI C_TGCA'G
Cuts at: 830 2399 4322 830
Size: 1569 1923 3478
Fragments arranged by size:
3478 1923 1569
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6970
PvuII CAG'CTG
Cuts at: 3499 3844 3499
Size: 345 6625
SalI G'TCGA_C
Cuts at: 1936 4312 1936
Size: 2376 4594
SanDI GG'GwC_CC
Cuts at: 2633 2633
Size: 6970
SapI GCTCTTCn'nnn_
Cuts at: 2199 2199
Size: 6970
SbfI CC_TGCA'GG
Cuts at: 4322 4322
Size: 6970
ScaI AGT'ACT
Cuts at: 2275 4163 5218 6930 2275
Size: 1888 1055 1712 2315
Fragments arranged by size:
2315 1888 1712 1055
SexAI A'CCwGG_T
Cuts at: 3759 3759
Size: 6970
SfcI C'TryA_G
Cuts at: 826 1045 2395 4318 5453 6134 6325 826
Size: 219 1350 1923 1135 681 191 1471
Fragments arranged by size:
1923 1471 1350 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6970
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6970
SmaI CCC'GGG
Cuts at: 1872 4297 1872
Size: 2425 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3347 5074 5975 6237 820
Size: 197 926 1404 1727 901 262 1553
Fragments arranged by size:
1727 1553 1404 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4761 493
Size: 4268 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6970
SphI G_CATG'C
Cuts at: 1132 4328 1132
Size: 3196 3774
SspI AAT'ATT
Cuts at: 5 52 4894 5
Size: 47 4842 2081
Fragments arranged by size:
4842 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6970
StyI C'CwwG_G
Cuts at: 513 1065 2474 2605 3414 3580 3997 4409 513
Size: 552 1409 131 809 166 417 412 3074
Fragments arranged by size:
3074 1409 809 552 417 412 166 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4997 5014 5167 5352 5797 325
Size: 1255 3417 17 153 185 445 1498
Fragments arranged by size:
3417 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2273 3107 4161
Size: 274 80 33 51 1443 296 834 1054
Cuts at: 4161 5216 6928 96
Size: 1055 1712 138
Fragments arranged by size:
1712 1443 1055 1054 834 296 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2303 2335 4137 4169 6930 6962 2303
Size: 32 1802 32 2761 32 2311
Fragments arranged by size:
2761 2311 1802 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3761 1316
Size: 2445 4525
XbaI T'CTAG_A
Cuts at: 4306 4306
Size: 6970
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2251 2251
Size: 6970
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6970
XmaI C'CCGG_G
Cuts at: 1870 4295 1870
Size: 2425 4545
XmnI GAAnn'nnTTC
Cuts at: 3979 4080 5099 3979
Size: 101 1019 5850
Fragments arranged by size:
5850 1019 101
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4778 276
Size: 53 83 186 936 3244 2468
Fragments arranged by size:
3244 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SfiI
SfoI SrfI SwaI