Restriction map for
pFN21AE6715
(Circular) (Six-base) MAPSORT of: pFN21AE6715.seq Check: 7677 from: 1 to: 6949
pFN21AE6715 6949 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3505 3505
Size: 6949
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2492 4759 278
Size: 53 83 186 936 956 2267 2468
Fragments arranged by size:
2468 2267 956 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2160 4292 1937
Size: 223 2132 4594
Fragments arranged by size:
4594 2132 223
Acc65I G'GTAC_C
Cuts at: 4270 4270
Size: 6949
AclI AA'CG_TT
Cuts at: 1563 5076 5449 1563
Size: 3513 373 3063
Fragments arranged by size:
3513 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2693 3982 5012 6396 6664 1632
Size: 1061 1289 1030 1384 268 1917
Fragments arranged by size:
1917 1384 1289 1061 1030 268
AfeI AGC'GCT
Cuts at: 2918 2918
Size: 6949
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6752
AflIII A'CryG_T
Cuts at: 1203 2181 5848 6825 1203
Size: 978 3667 977 1327
Fragments arranged by size:
3667 1327 978 977
AgeI A'CCGG_T
Cuts at: 5780 5780
Size: 6949
AhdI GACnn_n'nnGTC
Cuts at: 2041 5678 2041
Size: 3637 3312
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6949
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2363 2549 1184
Size: 1179 186 5584
Fragments arranged by size:
5584 1179 186
AlwNI CAG_nnn'CTG
Cuts at: 3594 3967 4036 4692 6264 3594
Size: 373 69 656 1572 4279
Fragments arranged by size:
4279 1572 656 373 69
ApaLI G'TGCA_C
Cuts at: 5006 6162 5006
Size: 1156 5793
ApoI r'AATT_y
Cuts at: 1493 4258 4484 4636 6745 1493
Size: 2765 226 152 2109 1697
Fragments arranged by size:
2765 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5503 160
Size: 872 4471 1606
Fragments arranged by size:
4471 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6949
AvaI C'yCGr_G
Cuts at: 1870 1943 3177 3377 3508 3678 3701 3938 4274
Size: 73 1234 200 131 170 23 237 336
Cuts at: 4274 1870
Size: 4545
Fragments arranged by size:
4545 1234 336 237 200 170 131 73
23
BamHI G'GATC_C
Cuts at: 4279 4646 4279
Size: 367 6582
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2192 2961 2996 3395 3638
Size: 325 217 627 405 769 35 399 243
Cuts at: 3638 3905 4270 5725 618
Size: 267 365 1455 1842
Fragments arranged by size:
1842 1455 769 627 405 399 365 325
267 243 217 35
BanII G_rGCy'C
Cuts at: 1391 2732 2931 3377 3620 4023 4068 4158 4175
Size: 1341 199 446 243 403 45 90 17
Cuts at: 4175 4268 1391
Size: 93 4072
Fragments arranged by size:
4072 1341 446 403 243 199 93 90
45 17
BbeI G_GCGC'C
Cuts at: 2965 3909 2965
Size: 944 6005
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5978
BbvCI CC'TCA_GC
Cuts at: 2811 3860 2811
Size: 1049 5900
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5140 5174 1903
Size: 34 3203 34 3678
Fragments arranged by size:
3678 3203 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4841 6051 4841
Size: 1210 5739
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6949
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2106 2552 2822
Size: 107 122 71 631 605 434 446 270
Cuts at: 2822 3107 3792 5560 136
Size: 285 685 1768 1525
Fragments arranged by size:
1768 1525 685 631 605 446 434 285
270 122 107 71
BglII A'GATC_T
Cuts at: 6944 6944
Size: 6949
BlpI GC'TnA_GC
Cuts at: 3114 3827 4174 4366 3114
Size: 713 347 192 5697
Fragments arranged by size:
5697 713 347 192
Bme1580I G_kGCm'C
Cuts at: 1790 2195 3398 3643 5010 6166 1790
Size: 405 1203 245 1367 1156 2573
Fragments arranged by size:
2573 1367 1203 1156 405 245
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6949
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4680 5638 442
Size: 416 53 83 399 24 3263 958 1753
Fragments arranged by size:
3263 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6949
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2392 2392
Size: 6949
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2249 2356 3314 3581 3604 3781 3827 5609
Size: 1010 107 958 267 23 177 46 1782
Cuts at: 5609 1239
Size: 2579
Fragments arranged by size:
2579 1782 1010 958 267 177 107 46
23
Bpu10I CC'TnA_GC
Cuts at: 2811 3860 2811
Size: 1049 5900
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2609 2915 4142 5074 5939 6237 2609
Size: 306 1227 932 865 298 3321
Fragments arranged by size:
3321 1227 932 865 306 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2363 2855 5612 882
Size: 617 864 492 2757 2219
Fragments arranged by size:
2757 2219 864 617 492
BsaAI yAC'GTr
Cuts at: 493 1204 2184 2869 3753 4740 493
Size: 711 980 685 884 987 2702
Fragments arranged by size:
2702 987 980 884 711 685
BsaBI GATnn'nnATC
Cuts at: 6943 6943
Size: 6949
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2088 2157 2433 2489
Size: 53 83 186 936 555 69 276 56
Cuts at: 2489 2544 2847 2962 3906 4756 5138 5773 275
Size: 55 303 115 944 850 382 635 1451
Fragments arranged by size:
1451 944 936 850 635 555 382 303
276 186 115 83 69 56 55 53
BsaWI w'CCGG_w
Cuts at: 3837 5382 5780 6054 6201 6612 3837
Size: 1545 398 274 147 411 4174
Fragments arranged by size:
4174 1545 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2597 2627 2771 2801 2879
Size: 30 165 30 1194 30 144 30 78
Cuts at: 2879 2909 2999 3029 1178
Size: 30 90 30 5098
Fragments arranged by size:
5098 1194 165 144 90 78 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3020 3239 3545 3617 3833 4000 4154 1186
Size: 1834 219 306 72 216 167 154 3981
Fragments arranged by size:
3981 1834 306 219 216 167 154 72
BseYI C'CCAG_C
Cuts at: 2058 2152 2171 3594 4051 6152 6648 2058
Size: 94 19 1423 457 2101 496 2359
Fragments arranged by size:
2359 2101 1423 496 457 94 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2077 3980 4049 1163
Size: 573 341 1903 69 4063
Fragments arranged by size:
4063 1903 573 341 69
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2574 4399 5160 6188 1540
Size: 275 186 573 1825 761 1028 2301
Fragments arranged by size:
2301 1825 1028 761 573 275 186
BsiHKAI G_wGCw'C
Cuts at: 2517 2598 2732 2759 4158 5010 5095 6166 2517
Size: 81 134 27 1399 852 85 1071 3300
Fragments arranged by size:
3300 1399 1071 852 134 85 81 27
BsmI GAATG_Cn'
Cuts at: 4460 4553 4460
Size: 93 6856
BsmBI CGTCTCn'nnnn_
Cuts at: 3863 3863
Size: 6949
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2195 2517 2598 2732 2759 2931 3377
Size: 399 405 322 81 134 27 172 446
Cuts at: 3377 3398 3620 3643 4023 4068 4158 4175 4268
Size: 21 222 23 380 45 90 17 93
Cuts at: 4268 5010 5095 6166 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 446 405 399 380 322
222 172 134 93 90 85 81 45
27 23 21 17
BspEI T'CCGG_A
Cuts at: 6612 6612
Size: 6949
BspHI T'CATG_A
Cuts at: 4838 6568 4838
Size: 1730 5219
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2010 3505 4304 844
Size: 1166 1495 799 3489
Fragments arranged by size:
3489 1495 1166 799
BsrBI CCG'CTC
Cuts at: 1394 1823 2475 3118 3535 3724 4836 1394
Size: 429 652 643 417 189 1112 3507
Fragments arranged by size:
3507 1112 652 643 429 417 189
BsrDI GCAATG_nn'
Cuts at: 65 1248 5444 5618 65
Size: 1183 4196 174 1396
Fragments arranged by size:
4196 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2096 2123 2561 2958 4003 5593 5780
Size: 110 286 27 438 397 1045 1590 187
Cuts at: 5780 1700
Size: 2869
Fragments arranged by size:
2869 1590 1045 438 397 286 187 110
27
BsrGI T'GTAC_A
Cuts at: 96 2250 96
Size: 2154 4795
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6949
BssSI C'ACGA_G
Cuts at: 2128 2597 3659 5009 6021 2128
Size: 469 1062 1350 1012 3056
Fragments arranged by size:
3056 1350 1062 1012 469
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3967 794
Size: 3173 3776
BstBI TT'CG_AA
Cuts at: 5753 5753
Size: 6949
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6949
BstXI CCAn_nnnn'nTGG
Cuts at: 2800 3805 2800
Size: 1005 5944
BstYI r'GATC_y
Cuts at: 1972 2619 3078 4279 4646 5031 5048 6500 6586
Size: 647 459 1201 367 385 17 1452 86
Cuts at: 6586 6598 6944 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1201 647 459 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2483 2483
Size: 6949
BtgI C'CryG_G
Cuts at: 513 1065 2083 2137 2215 2463 2581 2769 2965
Size: 552 1018 54 78 248 118 188 196
Cuts at: 2965 3456 3716 4014 513
Size: 491 260 298 3448
Fragments arranged by size:
3448 1018 552 491 298 260 248 196
188 118 78 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6210
Fragments arranged by size:
6210 683 56
BtsI GCAGTG_nn'
Cuts at: 3246 4465 5259 5279 3246
Size: 1219 794 20 4916
Fragments arranged by size:
4916 1219 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6949
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6914
DraI TTT'AAA
Cuts at: 4253 4253
Size: 6949
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5956 809
Size: 507 4640 1802
Fragments arranged by size:
4640 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2080 2460 2559
Size: 54 1425 50 275 18 250 380 99
Cuts at: 2559 2571 2706 2772 3459 4396 5285 6914 8
Size: 12 135 66 687 937 889 1629 43
Fragments arranged by size:
1629 1425 937 889 687 380 275 250
135 99 66 54 50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2571 4396 1537
Size: 275 759 1825 4090
Fragments arranged by size:
4090 1825 759 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4879 1353
Size: 3526 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3742 5536 5910 6056 387
Size: 1064 138 2153 1794 374 146 1280
Fragments arranged by size:
2153 1794 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2730 4156 2730
Size: 1426 5523
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2249 2356 2693 3314 3581 3604 3781
Size: 393 617 107 337 621 267 23 177
Cuts at: 3781 3827 3982 5012 5609 6396 6664 1239
Size: 46 155 1030 597 787 268 1524
Fragments arranged by size:
1524 1030 787 621 617 597 393 337
268 267 177 155 107 46 23
EcoNI CCTnn'n_nnAGG
Cuts at: 2804 3365 3859 2804
Size: 561 494 5894
Fragments arranged by size:
5894 561 494
EcoO109I rG'GnC_Cy
Cuts at: 2043 2331 2388 3165 2043
Size: 288 57 777 5827
Fragments arranged by size:
5827 777 288 57
EcoRI G'AATT_C
Cuts at: 4258 4636 4258
Size: 378 6571
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4012 4012
Size: 6949
FseI GG_CCGG'CC
Cuts at: 4007 4007
Size: 6949
FspI TGC'GCA
Cuts at: 2507 2681 5455 2507
Size: 174 2774 4001
Fragments arranged by size:
4001 2774 174
HaeII r_GCGC'y
Cuts at: 1678 2215 2287 2534 2656 2920 2965 3909 4181
Size: 537 72 247 122 264 45 944 272
Cuts at: 4181 6096 1678
Size: 1915 2531
Fragments arranged by size:
2531 1915 944 537 272 264 247 122
72 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2027 2059 2771 2803 2879
Size: 32 135 32 455 32 712 32 76
Cuts at: 2879 2911 3329 3361 4145 4177 5590 5622 5664
Size: 32 418 32 784 32 1413 32 42
Cuts at: 5664 5696 1373
Size: 32 2626
Fragments arranged by size:
2626 1413 784 712 455 418 135 76
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4293 4539 669
Size: 988 281 2355 246 3079
Fragments arranged by size:
3079 2355 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6949
HpaI GTT'AAC
Cuts at: 4539 4539
Size: 6949
KasI G'GCGC_C
Cuts at: 2961 3905 2961
Size: 944 6005
KpnI G_GTAC'C
Cuts at: 4274 4274
Size: 6949
MfeI C'AATT_G
Cuts at: 4548 4548
Size: 6949
MluI A'CGCG_T
Cuts at: 6825 6825
Size: 6949
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2667 3432 4158 6063 6247 1305
Size: 1362 765 726 1905 184 2007
Fragments arranged by size:
2007 1905 1362 765 726 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2708 10
Size: 54 1425 343 876 4251
Fragments arranged by size:
4251 1425 876 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2082 3338 3551 4907 5266 5425 518
Size: 648 916 1256 213 1356 359 159 2042
Fragments arranged by size:
2042 1356 1256 916 648 359 213 159
MspA1I CmG'CkG
Cuts at: 1606 2175 2465 2583 3113 3458 3594 3636 3718
Size: 569 290 118 530 345 136 42 82
Cuts at: 3718 4016 4365 5042 6190 6435 1606
Size: 298 349 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 569 530 349 345 298
290 245 136 118 82 42
NaeI GCC'GGC
Cuts at: 2563 4005 2563
Size: 1442 5507
NarI GG'CG_CC
Cuts at: 2962 3906 2962
Size: 944 6005
NcoI C'CATG_G
Cuts at: 513 1065 2083 2965 513
Size: 552 1018 882 4497
Fragments arranged by size:
4497 1018 882 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6949
NgoMIV G'CCGG_C
Cuts at: 2561 4003 2561
Size: 1442 5507
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6949
NotI GC'GGCC_GC
Cuts at: 4396 4396
Size: 6949
NspI r_CATG'y
Cuts at: 1132 1229 2419 4307 5852 1132
Size: 97 1190 1888 1545 2229
Fragments arranged by size:
2229 1888 1545 1190 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6949
PciI A'CATG_T
Cuts at: 5848 5848
Size: 6949
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6949
PfoI T'CCnGG_A
Cuts at: 2988 3161 3558 2988
Size: 173 397 6379
Fragments arranged by size:
6379 397 173
PmeI GTTT'AAAC
Cuts at: 4253 4253
Size: 6949
PmlI CAC'GTG
Cuts at: 3753 3753
Size: 6949
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5018 5050 6531 6563 5018
Size: 32 1481 32 5404
Fragments arranged by size:
5404 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2043 2331 2388 3165 2043
Size: 288 57 777 5827
Fragments arranged by size:
5827 777 288 57
PshAI GACnn'nnGTC
Cuts at: 1613 3970 1613
Size: 2357 4592
PsiI TTA'TAA
Cuts at: 4519 4519
Size: 6949
PspXI vC'TCGA_Gb
Cuts at: 3938 3938
Size: 6949
PstI C_TGCA'G
Cuts at: 830 2352 3366 3438 3816 4301 830
Size: 1522 1014 72 378 485 3478
Fragments arranged by size:
3478 1522 1014 485 378 72
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6949
PvuII CAG'CTG
Cuts at: 3594 3594
Size: 6949
SacI G_AGCT'C
Cuts at: 2732 4158 2732
Size: 1426 5523
SacII CC_GC'GG
Cuts at: 2466 2584 3459 3719 4017 2466
Size: 118 875 260 298 5398
Fragments arranged by size:
5398 875 298 260 118
SalI G'TCGA_C
Cuts at: 1936 4291 1936
Size: 2355 4594
SbfI CC_TGCA'GG
Cuts at: 4301 4301
Size: 6949
ScaI AGT'ACT
Cuts at: 5197 6909 5197
Size: 1712 5237
SfcI C'TryA_G
Cuts at: 826 1045 2348 3362 3434 3812 3892 4297 5432
Size: 219 1303 1014 72 378 80 405 1135
Cuts at: 5432 6113 6304 826
Size: 681 191 1471
Fragments arranged by size:
1471 1303 1135 1014 681 405 378 219
191 80 72
SfiI GGCCn_nnn'nGGCC
Cuts at: 2106 2106
Size: 6949
SfoI GGC'GCC
Cuts at: 2963 3907 2963
Size: 944 6005
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6949
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6949
SmaI CCC'GGG
Cuts at: 1872 3703 4276 1872
Size: 1831 573 4545
Fragments arranged by size:
4545 1831 573
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2624 2930 3508 3938 4157 5053
Size: 197 926 681 306 578 430 219 896
Cuts at: 5053 5954 6216 820
Size: 901 262 1553
Fragments arranged by size:
1553 926 901 896 681 578 430 306
262 219 197
SnaBI TAC'GTA
Cuts at: 493 4740 493
Size: 4247 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6949
SphI G_CATG'C
Cuts at: 1132 2419 4307 1132
Size: 1287 1888 3774
Fragments arranged by size:
3774 1888 1287
SspI AAT'ATT
Cuts at: 5 52 4873 5
Size: 47 4821 2081
Fragments arranged by size:
4821 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6949
StyI C'CwwG_G
Cuts at: 513 1065 2083 2103 2110 2965 3000 3975 4388
Size: 552 1018 20 7 855 35 975 413
Cuts at: 4388 513
Size: 3074
Fragments arranged by size:
3074 1018 975 855 552 413 35 20
7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2236 2381 4976 4993 5146 5331 5776
Size: 1255 656 145 2595 17 153 185 445
Cuts at: 5776 325
Size: 1498
Fragments arranged by size:
2595 1498 1255 656 445 185 153 145
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2250 3015 5195
Size: 274 80 33 51 1443 273 765 2180
Cuts at: 5195 6907 96
Size: 1712 138
Fragments arranged by size:
2180 1712 1443 765 274 273 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2597 2629 6909 6941 2597
Size: 32 4280 32 2605
Fragments arranged by size:
4280 2605 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3733 1316
Size: 2417 4532
XbaI T'CTAG_A
Cuts at: 4126 4285 4126
Size: 159 6790
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2408 2701 2797 2408
Size: 293 96 6560
Fragments arranged by size:
6560 293 96
XhoI C'TCGA_G
Cuts at: 1943 3508 3938 1943
Size: 1565 430 4954
Fragments arranged by size:
4954 1565 430
XmaI C'CCGG_G
Cuts at: 1870 3701 4274 1870
Size: 1831 573 4545
Fragments arranged by size:
4545 1831 573
XmnI GAAnn'nnTTC
Cuts at: 5078 5078
Size: 6949
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2490 4757 276
Size: 53 83 186 936 956 2267 2468
Fragments arranged by size:
2468 2267 956 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I
DraIII EcoRV FspAI NruI NsiI PacI PspOMI PsrI
RsrII SanDI SapI SexAI SrfI SwaI