Restriction map for
pFN21AE6714
(Circular) (Six-base) MAPSORT of: pFN21AE6714.seq Check: 5288 from: 1 to: 8257
pFN21AE6714 8257 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2314 2314
Size: 8257
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6067 278
Size: 53 83 186 936 4531 2468
Fragments arranged by size:
4531 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5600 1937
Size: 3663 4594
Acc65I G'GTAC_C
Cuts at: 5578 5578
Size: 8257
AclI AA'CG_TT
Cuts at: 1563 5556 6384 6757 1563
Size: 3993 828 373 3063
Fragments arranged by size:
3993 3063 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2849 2906 3979 4839 6320 7704 7972 1632
Size: 1217 57 1073 860 1481 1384 268 1917
Fragments arranged by size:
1917 1481 1384 1217 1073 860 268 57
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8060
AflIII A'CryG_T
Cuts at: 1203 7156 8133 1203
Size: 5953 977 1327
Fragments arranged by size:
5953 1327 977
AgeI A'CCGG_T
Cuts at: 7088 7088
Size: 8257
AhdI GACnn_n'nnGTC
Cuts at: 6986 6986
Size: 8257
AleI CACnn'nnGTG
Cuts at: 1166 2057 3428 3626 3662 5075 1166
Size: 891 1371 198 36 1413 4348
Fragments arranged by size:
4348 1413 1371 891 198 36
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3104 5057 1184
Size: 1920 1953 4384
Fragments arranged by size:
4384 1953 1920
AlwNI CAG_nnn'CTG
Cuts at: 2726 3221 4863 5438 5548 6000 7572 2726
Size: 495 1642 575 110 452 1572 3411
Fragments arranged by size:
3411 1642 1572 575 495 452 110
ApaI G_GGCC'C
Cuts at: 4680 4680
Size: 8257
ApaLI G'TGCA_C
Cuts at: 6314 7470 6314
Size: 1156 7101
ApoI r'AATT_y
Cuts at: 1493 4148 4526 4853 5566 5792 5944 8053 1493
Size: 2655 378 327 713 226 152 2109 1697
Fragments arranged by size:
2655 2109 1697 713 378 327 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6811 160
Size: 872 5779 1606
Fragments arranged by size:
5779 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8257
AvaI C'yCGr_G
Cuts at: 1870 1943 4983 5458 5582 1870
Size: 73 3040 475 124 4545
Fragments arranged by size:
4545 3040 475 124 73
AvrII C'CTAG_G
Cuts at: 4373 4373
Size: 8257
BamHI G'GATC_C
Cuts at: 2429 3055 3886 4600 5587 5954 2429
Size: 626 831 714 987 367 4732
Fragments arranged by size:
4732 987 831 714 626 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2358 2708 3342 4717 5197
Size: 325 217 627 571 350 634 1375 480
Cuts at: 5197 5578 7033 618
Size: 381 1455 1842
Fragments arranged by size:
1842 1455 1375 634 627 571 480 381
350 325 217
BanII G_rGCy'C
Cuts at: 1391 2162 2465 3516 3732 3849 4680 5576 1391
Size: 771 303 1051 216 117 831 896 4072
Fragments arranged by size:
4072 1051 896 831 771 303 216 117
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3795 4185 4509 4827 928
Size: 971 1896 390 324 318 4358
Fragments arranged by size:
4358 1896 971 390 324 318
BbvCI CC'TCA_GC
Cuts at: 3092 4554 4662 3092
Size: 1462 108 6687
Fragments arranged by size:
6687 1462 108
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6448 6482 1903
Size: 34 4511 34 3678
Fragments arranged by size:
4511 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6149 7359 6149
Size: 1210 7047
BclI T'GATC_A
Cuts at: 1548 5050 5550 1548
Size: 3502 500 4255
Fragments arranged by size:
4255 3502 500
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2631 2723 2814
Size: 107 122 71 631 605 959 92 91
Cuts at: 2814 6868 136
Size: 4054 1525
Fragments arranged by size:
4054 1525 959 631 605 122 107 92
91 71
BglII A'GATC_T
Cuts at: 8252 8252
Size: 8257
BlpI GC'TnA_GC
Cuts at: 2488 5674 2488
Size: 3186 5071
Bme1580I G_kGCm'C
Cuts at: 1790 2266 2713 2810 3578 4680 4931 5457 6318
Size: 476 447 97 768 1102 251 526 861
Cuts at: 6318 7474 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1102 861 768 526 476 447
251 97
BmgBI CAC'GTC
Cuts at: 1318 3289 1318
Size: 1971 6286
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2080 2517 3209
Size: 416 53 83 399 24 663 437 692
Cuts at: 3209 4719 5222 5413 5988 6946 442
Size: 1510 503 191 575 958 1753
Fragments arranged by size:
1753 1510 958 692 663 575 503 437
416 399 191 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8257
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3000 4027 4998 5363 3000
Size: 1027 971 365 5894
Fragments arranged by size:
5894 1027 971 365
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2174 2479 2497 3827 4226 4364 5042 5299
Size: 935 305 18 1330 399 138 678 257
Cuts at: 5299 6917 1239
Size: 1618 2579
Fragments arranged by size:
2579 1618 1330 935 678 399 305 257
138 18
Bpu10I CC'TnA_GC
Cuts at: 3092 4554 4662 3092
Size: 1462 108 6687
Fragments arranged by size:
6687 1462 108
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6382 7247 7545 6382
Size: 865 298 7094
Fragments arranged by size:
7094 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2475 3746 6920 882
Size: 617 976 1271 3174 2219
Fragments arranged by size:
3174 2219 1271 976 617
BsaAI yAC'GTr
Cuts at: 493 1204 6048 493
Size: 711 4844 2702
Fragments arranged by size:
4844 2702 711
BsaBI GATnn'nnATC
Cuts at: 8251 8251
Size: 8257
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2229 2750 4548 6064
Size: 53 83 186 936 696 521 1798 1516
Cuts at: 6064 6446 7081 275
Size: 382 635 1451
Fragments arranged by size:
1798 1516 1451 936 696 635 521 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 4281 4740 6690 7088 7362 7509 7920 4281
Size: 459 1950 398 274 147 411 4618
Fragments arranged by size:
4618 1950 459 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3386 3416 5015 5045 1178
Size: 30 165 30 1983 30 1599 30 4390
Fragments arranged by size:
4390 1983 1599 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4145 4234 4679 4990 5302 5476 1186
Size: 2959 89 445 311 312 174 3967
Fragments arranged by size:
3967 2959 445 312 311 174 89
BseYI C'CCAG_C
Cuts at: 2123 2713 2969 3116 3149 3174 4490 5413 7460
Size: 590 256 147 33 25 1316 923 2047
Cuts at: 7460 7956 2123
Size: 496 2424
Fragments arranged by size:
2424 2047 1316 923 590 496 256 147
33 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2578 3336 3377 3424 3463 4749 5053
Size: 573 842 758 41 47 39 1286 304
Cuts at: 5053 1163
Size: 4367
Fragments arranged by size:
4367 1286 842 758 573 304 47 41
39
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5284 5707 6468 7496 1540
Size: 275 186 3283 423 761 1028 2301
Fragments arranged by size:
3283 2301 1028 761 423 275 186
BsiHKAI G_wGCw'C
Cuts at: 2489 3621 4080 4982 6318 6403 7474 2489
Size: 1132 459 902 1336 85 1071 3272
Fragments arranged by size:
3272 1336 1132 1071 902 459 85
BsiWI C'GTAC_G
Cuts at: 2407 2407
Size: 8257
BsmI GAATG_Cn'
Cuts at: 2647 3268 5768 5861 2647
Size: 621 2500 93 5043
Fragments arranged by size:
5043 2500 621 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2162 2266 2465 2489 2713 2810 3516
Size: 399 372 104 199 24 224 97 706
Cuts at: 3516 3578 3621 3732 3849 4080 4680 4931 4982
Size: 62 43 111 117 231 600 251 51
Cuts at: 4982 5457 5576 6318 6403 7474 1391
Size: 475 119 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 706 600 475 399 372
251 231 224 199 119 117 111 104
97 85 62 51 43 24
BspEI T'CCGG_A
Cuts at: 7920 7920
Size: 8257
BspHI T'CATG_A
Cuts at: 5322 6146 7876 5322
Size: 824 1730 5703
Fragments arranged by size:
5703 1730 824
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2314 2630 5612 844
Size: 1470 316 2982 3489
Fragments arranged by size:
3489 2982 1470 316
BsrBI CCG'CTC
Cuts at: 1394 1823 2221 6144 1394
Size: 429 398 3923 3507
Fragments arranged by size:
3923 3507 429 398
BsrDI GCAATG_nn'
Cuts at: 65 1248 3047 4678 5338 6752 6926 65
Size: 1183 1799 1631 660 1414 174 1396
Fragments arranged by size:
1799 1631 1414 1396 1183 660 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5283 6901 7088 1700
Size: 110 3473 1618 187 2869
Fragments arranged by size:
3473 2869 1618 187 110
BsrGI T'GTAC_A
Cuts at: 96 2603 3534 96
Size: 2507 931 4819
Fragments arranged by size:
4819 2507 931
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8257
BssSI C'ACGA_G
Cuts at: 6317 7329 6317
Size: 1012 7245
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4257 794
Size: 3463 4794
BstBI TT'CG_AA
Cuts at: 7061 7061
Size: 8257
BstEII G'GTnAC_C
Cuts at: 1184 4006 1184
Size: 2822 5435
BstXI CCAn_nnnn'nTGG
Cuts at: 3407 3430 3739 5225 3407
Size: 23 309 1486 6439
Fragments arranged by size:
6439 1486 309 23
BstYI r'GATC_y
Cuts at: 1972 2429 3055 3396 3886 4311 4600 5587 5954
Size: 457 626 341 490 425 289 987 367
Cuts at: 5954 6339 6356 7808 7894 7906 8252 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 987 626 490 457 425 385
367 346 341 289 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2719 2719
Size: 8257
BtgI C'CryG_G
Cuts at: 513 1065 2004 2332 2465 3747 3795 4604 513
Size: 552 939 328 133 1282 48 809 4166
Fragments arranged by size:
4166 1282 939 809 552 328 133 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7518
Fragments arranged by size:
7518 683 56
BtsI GCAGTG_nn'
Cuts at: 2566 2584 3412 4515 5041 5773 6567 6587 2566
Size: 18 828 1103 526 732 794 20 4236
Fragments arranged by size:
4236 1103 828 794 732 526 20 18
ClaI AT'CG_AT
Cuts at: 1554 4765 1554
Size: 3211 5046
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5017 5052 529
Size: 35 4453 35 3734
Fragments arranged by size:
4453 3734 35 35
DraI TTT'AAA
Cuts at: 5561 5561
Size: 8257
DraIII CAC_nnn'GTG
Cuts at: 4727 4727
Size: 8257
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7264 809
Size: 507 5948 1802
Fragments arranged by size:
5948 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2320 2383 3432
Size: 54 1425 50 275 18 490 63 1049
Cuts at: 3432 3526 4638 4838 5262 5281 5488 5704 6593
Size: 94 1112 200 424 19 207 216 889
Cuts at: 6593 8222 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1112 1049 889 490 424 275
216 207 200 94 63 54 50 43
19 18
EagI C'GGCC_G
Cuts at: 1537 1812 5281 5704 1537
Size: 275 3469 423 4090
Fragments arranged by size:
4090 3469 423 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2092 2568 4707 5321 5443 6187 1353
Size: 739 476 2139 614 122 744 3423
Fragments arranged by size:
3423 2139 744 739 614 476 122
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4954 6844 7218 7364 387
Size: 1064 138 3365 1890 374 146 1280
Fragments arranged by size:
3365 1890 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2174 2479 2497 2849 2906 3827 3979
Size: 393 542 305 18 352 57 921 152
Cuts at: 3979 4226 4364 4839 5042 5299 6320 6917 7704
Size: 247 138 475 203 257 1021 597 787
Cuts at: 7704 7972 1239
Size: 268 1524
Fragments arranged by size:
1524 1021 921 787 597 542 475 393
352 305 268 257 247 203 152 138
57 18
EcoNI CCTnn'n_nnAGG
Cuts at: 5078 5430 5461 5078
Size: 352 31 7874
Fragments arranged by size:
7874 352 31
EcoO109I rG'GnC_Cy
Cuts at: 4275 4377 4409 4419 4860 5251 4275
Size: 102 32 10 441 391 7281
Fragments arranged by size:
7281 441 391 102 32 10
EcoRI G'AATT_C
Cuts at: 4853 5566 5944 4853
Size: 713 378 7166
Fragments arranged by size:
7166 713 378
FspI TGC'GCA
Cuts at: 6763 6763
Size: 8257
HaeII r_GCGC'y
Cuts at: 1678 5497 7404 1678
Size: 3819 1907 2531
Fragments arranged by size:
3819 2531 1907
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2534 2566 2778 2810 3703
Size: 32 135 32 962 32 212 32 893
Cuts at: 3703 3735 3800 3832 3990 4022 4067 4099 4433
Size: 32 65 32 158 32 45 32 334
Cuts at: 4433 4448 4465 4480 5285 5297 5317 5329 6898
Size: 15 17 15 805 12 20 12 1569
Cuts at: 6898 6930 6972 7004 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1569 962 893 805 334 212 158
135 65 45 42 32 32 32 32
32 32 32 32 32 32 20 17
15 15 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3652 5146 5167 5601 5847 669
Size: 988 281 1714 1494 21 434 246 3079
Fragments arranged by size:
3079 1714 1494 988 434 281 246 21
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8257
HpaI GTT'AAC
Cuts at: 5847 5847
Size: 8257
KpnI G_GTAC'C
Cuts at: 5582 5582
Size: 8257
MfeI C'AATT_G
Cuts at: 4542 5856 4542
Size: 1314 6943
MluI A'CGCG_T
Cuts at: 8133 8133
Size: 8257
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3701 4392 4565 7371 7555 1305
Size: 2396 691 173 2806 184 2007
Fragments arranged by size:
2806 2396 2007 691 184 173
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2322 2385 3434 3528 4640
Size: 54 1425 343 490 63 1049 94 1112
Cuts at: 4640 4840 5490 10
Size: 200 650 2777
Fragments arranged by size:
2777 1425 1112 1049 650 490 343 200
94 63 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2057 2939 3428 3626 3662 5075 5223
Size: 648 891 882 489 198 36 1413 148
Cuts at: 5223 6215 6574 6733 518
Size: 992 359 159 2042
Fragments arranged by size:
2042 1413 992 891 882 648 489 359
198 159 148 36
MspA1I CmG'CkG
Cuts at: 1606 2377 2492 3178 3447 3454 3914 4661 5012
Size: 771 115 686 269 7 460 747 351
Cuts at: 5012 5100 5673 6350 7498 7743 1606
Size: 88 573 677 1148 245 2120
Fragments arranged by size:
2120 1148 771 747 686 677 573 460
351 269 245 115 88 7
NcoI C'CATG_G
Cuts at: 513 1065 2004 2332 2465 3747 3795 513
Size: 552 939 328 133 1282 48 4975
Fragments arranged by size:
4975 1282 939 552 328 133 48
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8257
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8257
NotI GC'GGCC_GC
Cuts at: 5704 5704
Size: 8257
NspI r_CATG'y
Cuts at: 1132 1229 2665 3982 4710 5615 7160 1132
Size: 97 1436 1317 728 905 1545 2229
Fragments arranged by size:
2229 1545 1436 1317 905 728 97
PasI CC'CwG_GG
Cuts at: 1348 3769 5092 1348
Size: 2421 1323 4513
Fragments arranged by size:
4513 2421 1323
PciI A'CATG_T
Cuts at: 7156 7156
Size: 8257
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2022 2298 1108
Size: 914 276 7067
Fragments arranged by size:
7067 914 276
PfoI T'CCnGG_A
Cuts at: 2043 2701 3818 3882 2043
Size: 658 1117 64 6418
Fragments arranged by size:
6418 1117 658 64
PmeI GTTT'AAAC
Cuts at: 5561 5561
Size: 8257
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6326 6358 7839 7871 6326
Size: 32 1481 32 6712
Fragments arranged by size:
6712 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 5251 5251
Size: 8257
PshAI GACnn'nnGTC
Cuts at: 1613 2510 4061 1613
Size: 897 1551 5809
Fragments arranged by size:
5809 1551 897
PsiI TTA'TAA
Cuts at: 5827 5827
Size: 8257
PspOMI G'GGCC_C
Cuts at: 4676 4676
Size: 8257
PspXI vC'TCGA_Gb
Cuts at: 5458 5458
Size: 8257
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3965 3997 3965
Size: 32 8225
PstI C_TGCA'G
Cuts at: 830 3105 3642 5434 5609 830
Size: 2275 537 1792 175 3478
Fragments arranged by size:
3478 2275 1792 537 175
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8257
PvuII CAG'CTG
Cuts at: 2492 3178 3447 3914 4661 5012 5100 2492
Size: 686 269 467 747 351 88 5649
Fragments arranged by size:
5649 747 686 467 351 269 88
SalI G'TCGA_C
Cuts at: 1936 5599 1936
Size: 3663 4594
SapI GCTCTTCn'nnn_
Cuts at: 5321 5321
Size: 8257
SbfI CC_TGCA'GG
Cuts at: 5609 5609
Size: 8257
ScaI AGT'ACT
Cuts at: 3125 6505 8217 3125
Size: 3380 1712 3165
Fragments arranged by size:
3380 3165 1712
SexAI A'CCwGG_T
Cuts at: 5139 5139
Size: 8257
SfcI C'TryA_G
Cuts at: 826 1045 3101 3638 3829 5430 5605 6740 7421
Size: 219 2056 537 191 1601 175 1135 681
Cuts at: 7421 7612 826
Size: 191 1471
Fragments arranged by size:
2056 1601 1471 1135 681 537 219 191
191 175
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8257
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8257
SmaI CCC'GGG
Cuts at: 1872 5584 1872
Size: 3712 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5458 6361 7262 7524 820
Size: 197 926 3515 903 901 262 1553
Fragments arranged by size:
3515 1553 926 903 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6048 493
Size: 5555 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8257
SphI G_CATG'C
Cuts at: 1132 5615 1132
Size: 4483 3774
SspI AAT'ATT
Cuts at: 5 52 3898 6181 5
Size: 47 3846 2283 2081
Fragments arranged by size:
3846 2283 2081 47
StuI AGG'CCT
Cuts at: 1517 2021 1517
Size: 504 7753
StyI C'CwwG_G
Cuts at: 513 1065 2004 2016 2332 2465 3165 3426 3435
Size: 552 939 12 316 133 700 261 9
Cuts at: 3435 3747 3795 4373 4646 5202 5265 5443 5696
Size: 312 48 578 273 556 63 178 253
Cuts at: 5696 513
Size: 3074
Fragments arranged by size:
3074 939 700 578 556 552 316 312
273 261 253 178 133 63 48 12
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2014 2099 4738 6284 6301 6454 6639
Size: 1255 434 85 2639 1546 17 153 185
Cuts at: 6639 7084 325
Size: 445 1498
Fragments arranged by size:
2639 1546 1498 1255 445 434 185 153
85 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2603 2860 3123
Size: 274 80 33 51 1443 626 257 263
Cuts at: 3123 3534 5184 6503 8215 96
Size: 411 1650 1319 1712 138
Fragments arranged by size:
1712 1650 1443 1319 626 411 274 263
257 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3386 3418 3535 3567 5015 5047 8217 8249 3386
Size: 32 117 32 1448 32 3170 32 3394
Fragments arranged by size:
3394 3170 1448 117 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4397 1316
Size: 3081 5176
XbaI T'CTAG_A
Cuts at: 5593 5593
Size: 8257
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2330 2472 3146 3802 5498 2330
Size: 142 674 656 1696 5089
Fragments arranged by size:
5089 1696 674 656 142
XhoI C'TCGA_G
Cuts at: 1943 5458 1943
Size: 3515 4742
XmaI C'CCGG_G
Cuts at: 1870 5582 1870
Size: 3712 4545
XmnI GAAnn'nnTTC
Cuts at: 6386 6386
Size: 8257
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6065 276
Size: 53 83 186 936 4531 2468
Fragments arranged by size:
4531 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BfrBI BsmBI BstZ17I
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI RsrII SacI SacII
SanDI SfiI SfoI SrfI SwaI