Restriction map for
pFN21AE6713
(Circular) (Six-base) MAPSORT of: pFN21AE6713.seq Check: 297 from: 1 to: 6868
pFN21AE6713 6868 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2340 4678 278
Size: 53 83 186 936 804 2338 2468
Fragments arranged by size:
2468 2338 936 804 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4211 1937
Size: 2274 4594
Acc65I G'GTAC_C
Cuts at: 2709 3759 4189 2709
Size: 1050 430 5388
Fragments arranged by size:
5388 1050 430
AclI AA'CG_TT
Cuts at: 1563 4995 5368 1563
Size: 3432 373 3063
Fragments arranged by size:
3432 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3676 3739 4931 6315 6583 1632
Size: 2044 63 1192 1384 268 1917
Fragments arranged by size:
2044 1917 1384 1192 268 63
AfeI AGC'GCT
Cuts at: 2984 2984
Size: 6868
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6671
AflIII A'CryG_T
Cuts at: 1203 2167 5767 6744 1203
Size: 964 3600 977 1327
Fragments arranged by size:
3600 1327 977 964
AgeI A'CCGG_T
Cuts at: 5699 5699
Size: 6868
AhdI GACnn_n'nnGTC
Cuts at: 5597 5597
Size: 6868
AleI CACnn'nnGTG
Cuts at: 1166 2348 2774 3305 1166
Size: 1182 426 531 4729
Fragments arranged by size:
4729 1182 531 426
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6868
AlwNI CAG_nnn'CTG
Cuts at: 2742 2935 3181 3360 3418 4611 6183 2742
Size: 193 246 179 58 1193 1572 3427
Fragments arranged by size:
3427 1572 1193 246 193 179 58
ApaI G_GGCC'C
Cuts at: 2137 2650 3495 2137
Size: 513 845 5510
Fragments arranged by size:
5510 845 513
ApaLI G'TGCA_C
Cuts at: 3239 4925 6081 3239
Size: 1686 1156 4026
Fragments arranged by size:
4026 1686 1156
ApoI r'AATT_y
Cuts at: 1493 3065 4079 4177 4403 4555 6664 1493
Size: 1572 1014 98 226 152 2109 1697
Fragments arranged by size:
2109 1697 1572 1014 226 152 98
AseI AT'TA_AT
Cuts at: 160 1032 5422 160
Size: 872 4390 1606
Fragments arranged by size:
4390 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6868
AvaI C'yCGr_G
Cuts at: 1870 1943 2013 2025 2137 2218 2231 2917 3774
Size: 73 70 12 112 81 13 686 857
Cuts at: 3774 3977 4161 4193 1870
Size: 203 184 32 4545
Fragments arranged by size:
4545 857 686 203 184 112 81 73
70 32 13 12
BamHI G'GATC_C
Cuts at: 2511 4198 4565 2511
Size: 1687 367 4814
Fragments arranged by size:
4814 1687 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2019 2452 2709 3661 3759
Size: 325 217 627 232 433 257 952 98
Cuts at: 3759 3868 4189 5644 618
Size: 109 321 1455 1842
Fragments arranged by size:
1842 1455 952 627 433 325 321 257
232 217 109 98
BanII G_rGCy'C
Cuts at: 1391 2137 2292 2643 2650 2853 3129 3495 3966
Size: 746 155 351 7 203 276 366 471
Cuts at: 3966 4157 4187 1391
Size: 191 30 4072
Fragments arranged by size:
4072 746 471 366 351 276 203 191
155 30 7
BbeI G_GCGC'C
Cuts at: 2023 2023
Size: 6868
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3207 3547 928
Size: 971 1308 340 4249
Fragments arranged by size:
4249 1308 971 340
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5059 5093 1903
Size: 34 50 34 3038 34 3678
Fragments arranged by size:
3678 3038 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4760 5970 4760
Size: 1210 5658
BclI T'GATC_A
Cuts at: 1548 2904 1548
Size: 1356 5512
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2017 2122 5479
Size: 107 122 71 631 605 345 105 3357
Cuts at: 5479 136
Size: 1525
Fragments arranged by size:
3357 1525 631 605 345 122 107 105
71
BglII A'GATC_T
Cuts at: 6863 6863
Size: 6868
BlpI GC'TnA_GC
Cuts at: 2580 3162 3408 4285 2580
Size: 582 246 877 5163
Fragments arranged by size:
5163 877 582 246
Bme1580I G_kGCm'C
Cuts at: 1790 2137 2650 3140 3243 3495 3743 4929 6085
Size: 347 513 490 103 252 248 1186 1156
Cuts at: 6085 1790
Size: 2573
Fragments arranged by size:
2573 1186 1156 513 490 347 252 248
103
BmgBI CAC'GTC
Cuts at: 1318 3463 1318
Size: 2145 4723
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2559 4599 5557
Size: 416 53 83 399 24 1142 2040 958
Cuts at: 5557 442
Size: 1753
Fragments arranged by size:
2040 1753 1142 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6868
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2311 2447 3116 4021 4046 5528 1239
Size: 1072 136 669 905 25 1482 2579
Fragments arranged by size:
2579 1482 1072 905 669 136 25
Bpu10I CC'TnA_GC
Cuts at: 2855 3816 2855
Size: 961 5907
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2477 2993 4055 4993 5858 6156 2477
Size: 516 1062 938 865 298 3189
Fragments arranged by size:
3189 1062 938 865 516 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2208 2509 3668 5531 882
Size: 617 709 301 1159 1863 2219
Fragments arranged by size:
2219 1863 1159 709 617 301
BsaAI yAC'GTr
Cuts at: 493 1204 2168 3862 4659 493
Size: 711 964 1694 797 2702
Fragments arranged by size:
2702 1694 964 797 711
BsaBI GATnn'nnATC
Cuts at: 6862 6862
Size: 6868
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2020 2337 2406 2999
Size: 53 83 186 936 487 317 69 593
Cuts at: 2999 4675 5057 5692 275
Size: 1676 382 635 1451
Fragments arranged by size:
1676 1451 936 635 593 487 382 317
186 83 69 53
BsaWI w'CCGG_w
Cuts at: 5301 5699 5973 6120 6531 5301
Size: 398 274 147 411 5638
Fragments arranged by size:
5638 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2231 2261 2373 2403 2810
Size: 30 165 30 828 30 112 30 407
Cuts at: 2810 2840 1178
Size: 30 5206
Fragments arranged by size:
5206 828 407 165 112 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6868
BseYI C'CCAG_C
Cuts at: 2032 2814 3140 3312 3625 3631 4132 6071 6567
Size: 782 326 172 313 6 501 1939 496
Cuts at: 6567 2032
Size: 2333
Fragments arranged by size:
2333 1939 782 501 496 326 313 172
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2369 2948 2975 3373 1163
Size: 573 633 579 27 398 4658
Fragments arranged by size:
4658 633 579 573 398 27
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2682 4318 5079 6107 1540
Size: 275 186 681 1636 761 1028 2301
Fragments arranged by size:
2301 1636 1028 761 681 275 186
BsiHKAI G_wGCw'C
Cuts at: 2292 2643 2853 2916 3129 3243 3966 4929 5014
Size: 351 210 63 213 114 723 963 85
Cuts at: 5014 6085 2292
Size: 1071 3075
Fragments arranged by size:
3075 1071 963 723 351 213 210 114
85 63
BsmI GAATG_Cn'
Cuts at: 4379 4472 4379
Size: 93 6775
BsmBI CGTCTCn'nnnn_
Cuts at: 2328 2423 2328
Size: 95 6773
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2137 2292 2643 2650 2853 2916 3129
Size: 399 347 155 351 7 203 63 213
Cuts at: 3129 3140 3243 3495 3743 3966 4157 4187 4929
Size: 11 103 252 248 223 191 30 742
Cuts at: 4929 5014 6085 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 351 347 252 248
223 213 203 191 155 103 85 63
30 11 7
BspEI T'CCGG_A
Cuts at: 6531 6531
Size: 6868
BspHI T'CATG_A
Cuts at: 4757 6487 4757
Size: 1730 5138
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3120 4223 844
Size: 2276 1103 3489
Fragments arranged by size:
3489 2276 1103
BsrBI CCG'CTC
Cuts at: 1394 1823 2129 2158 2247 4035 4755 1394
Size: 429 306 29 89 1788 720 3507
Fragments arranged by size:
3507 1788 720 429 306 89 29
BsrDI GCAATG_nn'
Cuts at: 65 1248 3842 5363 5537 65
Size: 1183 2594 1521 174 1396
Fragments arranged by size:
2594 1521 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2225 2796 3327 3639 3664 5512 5699
Size: 110 415 571 531 312 25 1848 187
Cuts at: 5699 1700
Size: 2869
Fragments arranged by size:
2869 1848 571 531 415 312 187 110
25
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6868
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6868
BssSI C'ACGA_G
Cuts at: 3764 3873 4928 5940 3764
Size: 109 1055 1012 4692
Fragments arranged by size:
4692 1055 1012 109
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2935 3247 3418 794
Size: 2141 312 171 4244
Fragments arranged by size:
4244 2141 312 171
BstBI TT'CG_AA
Cuts at: 5672 5672
Size: 6868
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6868
BstXI CCAn_nnnn'nTGG
Cuts at: 2875 3379 2875
Size: 504 6364
BstYI r'GATC_y
Cuts at: 1972 2511 4198 4565 4950 4967 6419 6505 6517
Size: 539 1687 367 385 17 1452 86 12
Cuts at: 6517 6863 1972
Size: 346 1977
Fragments arranged by size:
1977 1687 1452 539 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3605 3605
Size: 6868
BtgI C'CryG_G
Cuts at: 513 1065 2409 3321 4105 513
Size: 552 1344 912 784 3276
Fragments arranged by size:
3276 1344 912 784 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6129
Fragments arranged by size:
6129 683 56
BtsI GCAGTG_nn'
Cuts at: 2381 4384 5178 5198 2381
Size: 2003 794 20 4051
Fragments arranged by size:
4051 2003 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6868
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6833
DraI TTT'AAA
Cuts at: 4172 4172
Size: 6868
DraIII CAC_nnn'GTG
Cuts at: 2398 3158 3247 3937 2398
Size: 760 89 690 5329
Fragments arranged by size:
5329 760 690 89
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2612 5875 809
Size: 507 1296 3263 1802
Fragments arranged by size:
3263 1802 1296 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2301 2412 2976
Size: 54 1425 50 275 18 471 111 564
Cuts at: 2976 3843 4315 5204 6833 8
Size: 867 472 889 1629 43
Fragments arranged by size:
1629 1425 889 867 564 472 471 275
111 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4315 1537
Size: 275 2503 4090
Fragments arranged by size:
4090 2503 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2369 2441 4798 1353
Size: 1016 72 2357 3423
Fragments arranged by size:
3423 2357 1016 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5455 5829 5975 387
Size: 1064 138 3866 374 146 1280
Fragments arranged by size:
3866 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2290 2641 2851 3127 3964 2290
Size: 351 210 276 837 5194
Fragments arranged by size:
5194 837 351 276 210
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2311 2447 3116 3676 3739 4021 4046
Size: 393 679 136 669 560 63 282 25
Cuts at: 4046 4931 5528 6315 6583 1239
Size: 885 597 787 268 1524
Fragments arranged by size:
1524 885 787 679 669 597 560 393
282 268 136 63 25
EcoNI CCTnn'n_nnAGG
Cuts at: 4161 4161
Size: 6868
EcoO109I rG'GnC_Cy
Cuts at: 2092 2133 2134 2647 2739 3178 3491 3492 3538
Size: 41 1 513 92 439 313 1 46
Cuts at: 3538 3609 3820 4066 2092
Size: 71 211 246 4894
Fragments arranged by size:
4894 513 439 313 246 211 92 71
46 41 1 1
EcoRI G'AATT_C
Cuts at: 3065 4079 4177 4555 3065
Size: 1014 98 378 5378
Fragments arranged by size:
5378 1014 378 98
FseI GG_CCGG'CC
Cuts at: 2229 2229
Size: 6868
FspI TGC'GCA
Cuts at: 5374 5374
Size: 6868
HaeII r_GCGC'y
Cuts at: 1678 2023 2033 2110 2986 6015 1678
Size: 345 10 77 876 3029 2531
Fragments arranged by size:
3029 2531 876 345 77 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2325 2357 5509 5541 5583
Size: 32 135 32 753 32 3152 32 42
Cuts at: 5583 5615 1373
Size: 32 2626
Fragments arranged by size:
3152 2626 753 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3508 4212 4458 669
Size: 988 281 1570 704 246 3079
Fragments arranged by size:
3079 1570 988 704 281 246
HindIII A'AGCT_T
Cuts at: 748 3038 748
Size: 2290 4578
HpaI GTT'AAC
Cuts at: 4458 4458
Size: 6868
KasI G'GCGC_C
Cuts at: 2019 2019
Size: 6868
KpnI G_GTAC'C
Cuts at: 2713 3763 4193 2713
Size: 1050 430 5388
Fragments arranged by size:
5388 1050 430
MfeI C'AATT_G
Cuts at: 4467 4467
Size: 6868
MluI A'CGCG_T
Cuts at: 6744 6744
Size: 6868
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2274 3321 5982 6166 1305
Size: 969 1047 2661 184 2007
Fragments arranged by size:
2661 2007 1047 969 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2303 2414 10
Size: 54 1425 343 471 111 4464
Fragments arranged by size:
4464 1425 471 343 111 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2348 2447 2774 3305 4826 5185 5344
Size: 648 1182 99 327 531 1521 359 159
Cuts at: 5344 518
Size: 2042
Fragments arranged by size:
2042 1521 1182 648 531 359 327 159
99
MspA1I CmG'CkG
Cuts at: 1606 2959 3316 3625 3804 4284 4961 6109 6354
Size: 1353 357 309 179 480 677 1148 245
Cuts at: 6354 1606
Size: 2120
Fragments arranged by size:
2120 1353 1148 677 480 357 309 245
179
NaeI GCC'GGC
Cuts at: 2227 3641 3666 2227
Size: 1414 25 5429
Fragments arranged by size:
5429 1414 25
NarI GG'CG_CC
Cuts at: 2020 2020
Size: 6868
NcoI C'CATG_G
Cuts at: 513 1065 3321 4105 513
Size: 552 2256 784 3276
Fragments arranged by size:
3276 2256 784 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6868
NgoMIV G'CCGG_C
Cuts at: 2225 3639 3664 2225
Size: 1414 25 5429
Fragments arranged by size:
5429 1414 25
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6868
NotI GC'GGCC_GC
Cuts at: 4315 4315
Size: 6868
NspI r_CATG'y
Cuts at: 1132 1229 2300 3062 3254 4226 5771 1132
Size: 97 1071 762 192 972 1545 2229
Fragments arranged by size:
2229 1545 1071 972 762 192 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6868
PciI A'CATG_T
Cuts at: 5767 5767
Size: 6868
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3937 1108
Size: 2829 4039
PfoI T'CCnGG_A
Cuts at: 2424 2507 2601 3093 2424
Size: 83 94 492 6199
Fragments arranged by size:
6199 492 94 83
PmeI GTTT'AAAC
Cuts at: 4172 4172
Size: 6868
PmlI CAC'GTG
Cuts at: 2168 2168
Size: 6868
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4937 4969 6450 6482 4937
Size: 32 1481 32 5323
Fragments arranged by size:
5323 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2092 2092
Size: 6868
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6868
PsiI TTA'TAA
Cuts at: 4438 4438
Size: 6868
PspOMI G'GGCC_C
Cuts at: 2133 2646 3491 2133
Size: 513 845 5510
Fragments arranged by size:
5510 845 513
PstI C_TGCA'G
Cuts at: 830 3336 4143 4220 830
Size: 2506 807 77 3478
Fragments arranged by size:
3478 2506 807 77
PvuI CG_AT'CG
Cuts at: 2001 2682 2001
Size: 681 6187
PvuII CAG'CTG
Cuts at: 2959 3316 3625 2959
Size: 357 309 6202
Fragments arranged by size:
6202 357 309
SacI G_AGCT'C
Cuts at: 2292 2643 2853 3129 3966 2292
Size: 351 210 276 837 5194
Fragments arranged by size:
5194 837 351 276 210
SalI G'TCGA_C
Cuts at: 1936 4210 1936
Size: 2274 4594
SapI GCTCTTCn'nnn_
Cuts at: 2369 2441 2369
Size: 72 6796
SbfI CC_TGCA'GG
Cuts at: 4220 4220
Size: 6868
ScaI AGT'ACT
Cuts at: 5116 6828 5116
Size: 1712 5156
SexAI A'CCwGG_T
Cuts at: 3154 3154
Size: 6868
SfcI C'TryA_G
Cuts at: 826 1045 2368 3332 4139 4216 5351 6032 6223
Size: 219 1323 964 807 77 1135 681 191
Cuts at: 6223 826
Size: 1471
Fragments arranged by size:
1471 1323 1135 964 807 681 219 191
77
SfoI GGC'GCC
Cuts at: 2021 2021
Size: 6868
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6868
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6868
SmaI CCC'GGG
Cuts at: 1872 2027 2139 2220 2233 2919 4195 1872
Size: 155 112 81 13 686 1276 4545
Fragments arranged by size:
4545 1276 686 155 112 81 13
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2492 3008 4070 4972 5873 6135
Size: 197 926 549 516 1062 902 901 262
Cuts at: 6135 820
Size: 1553
Fragments arranged by size:
1553 1062 926 902 901 549 516 262
197
SnaBI TAC'GTA
Cuts at: 493 4659 493
Size: 4166 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6868
SphI G_CATG'C
Cuts at: 1132 4226 1132
Size: 3094 3774
SspI AAT'ATT
Cuts at: 5 52 4792 5
Size: 47 4740 2081
Fragments arranged by size:
4740 2081 47
StuI AGG'CCT
Cuts at: 1517 3434 3983 1517
Size: 1917 549 4402
Fragments arranged by size:
4402 1917 549
StyI C'CwwG_G
Cuts at: 513 1065 2751 3321 3528 3708 4105 4307 513
Size: 552 1686 570 207 180 397 202 3074
Fragments arranged by size:
3074 1686 570 552 397 207 202 180
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2672 3802 4895 4912 5065 5250 5695
Size: 1255 1092 1130 1093 17 153 185 445
Cuts at: 5695 325
Size: 1498
Fragments arranged by size:
1498 1255 1130 1093 1092 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5114 6826 96
Size: 274 80 33 51 1443 3137 1712 138
Fragments arranged by size:
3137 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2084 2116 2231 2263 2373 2405 2810 2842 3335
Size: 32 115 32 110 32 405 32 493
Cuts at: 3335 3367 6828 6860 2084
Size: 32 3461 32 2092
Fragments arranged by size:
3461 2092 493 405 115 110 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6868
XbaI T'CTAG_A
Cuts at: 4204 4204
Size: 6868
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2422 3152 3622 2422
Size: 730 470 5668
Fragments arranged by size:
5668 730 470
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6868
XmaI C'CCGG_G
Cuts at: 1870 2025 2137 2218 2231 2917 4193 1870
Size: 155 112 81 13 686 1276 4545
Fragments arranged by size:
4545 1276 686 155 112 81 13
XmnI GAAnn'nnTTC
Cuts at: 4997 4997
Size: 6868
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2338 4676 276
Size: 53 83 186 936 804 2338 2468
Fragments arranged by size:
2468 2338 936 804 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BstZ17I EcoRV FalI FspAI NruI NsiI PacI
PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI