Restriction map for
pFN21AE6712
(Circular) (Six-base) MAPSORT of: pFN21AE6712.seq Check: 7182 from: 1 to: 7507
pFN21AE6712 7507 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4390 4751 4390
Size: 361 7146
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5317 278
Size: 53 83 186 936 3781 2468
Fragments arranged by size:
3781 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4695 4850 1937
Size: 2758 155 4594
Fragments arranged by size:
4594 2758 155
Acc65I G'GTAC_C
Cuts at: 3752 4828 3752
Size: 1076 6431
AclI AA'CG_TT
Cuts at: 1563 5634 6007 1563
Size: 4071 373 3063
Fragments arranged by size:
4071 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4038 4343 5570 6954 7222 1632
Size: 2406 305 1227 1384 268 1917
Fragments arranged by size:
2406 1917 1384 1227 305 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7310
AflIII A'CryG_T
Cuts at: 1203 6406 7383 1203
Size: 5203 977 1327
Fragments arranged by size:
5203 1327 977
AgeI A'CCGG_T
Cuts at: 6338 6338
Size: 7507
AhdI GACnn_n'nnGTC
Cuts at: 6236 6236
Size: 7507
AleI CACnn'nnGTG
Cuts at: 1166 2789 3071 1166
Size: 1623 282 5602
Fragments arranged by size:
5602 1623 282
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7507
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2075 2107 2075
Size: 32 7475
AlwNI CAG_nnn'CTG
Cuts at: 5250 6822 5250
Size: 1572 5935
ApaI G_GGCC'C
Cuts at: 4224 4224
Size: 7507
ApaLI G'TGCA_C
Cuts at: 5564 6720 5564
Size: 1156 6351
ApoI r'AATT_y
Cuts at: 1493 3599 4816 5042 5194 7303 1493
Size: 2106 1217 226 152 2109 1697
Fragments arranged by size:
2109 2106 1697 1217 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6061 160
Size: 872 5029 1606
Fragments arranged by size:
5029 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7507
AvaI C'yCGr_G
Cuts at: 1870 1943 2471 3089 3291 3419 3747 4023 4053
Size: 73 528 618 202 128 328 276 30
Cuts at: 4053 4832 1870
Size: 779 4545
Fragments arranged by size:
4545 779 618 528 328 276 202 128
73 30
BamHI G'GATC_C
Cuts at: 3839 4837 5204 3839
Size: 998 367 6142
Fragments arranged by size:
6142 998 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2075 2987 3632 3752 4484
Size: 325 217 627 288 912 645 120 732
Cuts at: 4484 4517 4616 4622 4828 6283 618
Size: 33 99 6 206 1455 1842
Fragments arranged by size:
1842 1455 912 732 645 627 325 288
217 206 120 99 33 6
BanII G_rGCy'C
Cuts at: 1391 3427 3731 4192 4202 4224 4601 4826 1391
Size: 2036 304 461 10 22 377 225 4072
Fragments arranged by size:
4072 2036 461 377 304 225 22 10
BbeI G_GCGC'C
Cuts at: 2991 2991
Size: 7507
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2725 928
Size: 971 826 5710
Fragments arranged by size:
5710 971 826
BbvCI CC'TCA_GC
Cuts at: 3278 3757 3278
Size: 479 7028
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2137 2171 3455 3489 5698 5732 1903
Size: 34 200 34 1284 34 2209 34 3678
Fragments arranged by size:
3678 2209 1284 200 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5399 6609 5399
Size: 1210 6297
BclI T'GATC_A
Cuts at: 1548 3945 1548
Size: 2397 5110
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6118 136
Size: 107 122 71 631 605 4446 1525
Fragments arranged by size:
4446 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7502 7502
Size: 7507
BlpI GC'TnA_GC
Cuts at: 2525 4924 2525
Size: 2399 5108
Bme1580I G_kGCm'C
Cuts at: 1790 3576 3635 3810 4224 4677 5568 6724 1790
Size: 1786 59 175 414 453 891 1156 2573
Fragments arranged by size:
2573 1786 1156 891 453 414 175 59
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7507
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3077 4648 5238
Size: 416 53 83 399 24 1660 1571 590
Cuts at: 5238 6196 442
Size: 958 1753
Fragments arranged by size:
1753 1660 1571 958 590 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2223 1056
Size: 1167 6340
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4054 4440 4054
Size: 386 7121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2041 2235 2348 2372 2618 2786 6167 1239
Size: 802 194 113 24 246 168 3381 2579
Fragments arranged by size:
3381 2579 802 246 194 168 113 24
Bpu10I CC'TnA_GC
Cuts at: 3278 3757 3278
Size: 479 7028
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3197 5632 6497 6795 3197
Size: 2435 865 298 3909
Fragments arranged by size:
3909 2435 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2243 3191 4170 4471 6170 882
Size: 617 744 948 979 301 1699 2219
Fragments arranged by size:
2219 1699 979 948 744 617 301
BsaAI yAC'GTr
Cuts at: 493 1204 4672 5298 493
Size: 711 3468 626 2702
Fragments arranged by size:
3468 2702 711 626
BsaBI GATnn'nnATC
Cuts at: 2966 7501 2966
Size: 4535 2972
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2988 3297 3459 4239
Size: 53 83 186 936 1455 309 162 780
Cuts at: 4239 4571 5314 5696 6331 275
Size: 332 743 382 635 1451
Fragments arranged by size:
1455 1451 936 780 743 635 382 332
309 186 162 83 53
BsaWI w'CCGG_w
Cuts at: 2252 4384 5940 6338 6612 6759 7170 2252
Size: 2132 1556 398 274 147 411 2589
Fragments arranged by size:
2589 2132 1556 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2579 2609 3171 3201 1178
Size: 30 165 30 1176 30 562 30 5484
Fragments arranged by size:
5484 1176 562 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2038 2303 1186
Size: 852 265 6390
Fragments arranged by size:
6390 852 265
BseYI C'CCAG_C
Cuts at: 2668 3789 4756 6710 7206 2668
Size: 1121 967 1954 496 2969
Fragments arranged by size:
2969 1954 1121 967 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2576 3261 3857 4356 4431 1163
Size: 573 840 685 596 499 75 4239
Fragments arranged by size:
4239 840 685 596 573 499 75
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2275 4957 5718 6746 1540
Size: 275 186 274 2682 761 1028 2301
Fragments arranged by size:
2682 2301 1028 761 275 274 186
BsiHKAI G_wGCw'C
Cuts at: 3007 3731 4202 4601 5568 5653 6724 3007
Size: 724 471 399 967 85 1071 3790
Fragments arranged by size:
3790 1071 967 724 471 399 85
BsmI GAATG_Cn'
Cuts at: 3475 5018 5111 3475
Size: 1543 93 5871
Fragments arranged by size:
5871 1543 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3288 3288
Size: 7507
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3007 3427 3576 3635 3731 3810 4192
Size: 399 1217 420 149 59 96 79 382
Cuts at: 4192 4202 4224 4601 4677 4826 5568 5653 6724
Size: 10 22 377 76 149 742 85 1071
Cuts at: 6724 1391
Size: 2174
Fragments arranged by size:
2174 1217 1071 742 420 399 382 377
149 149 96 85 79 76 59 22
10
BspEI T'CCGG_A
Cuts at: 7170 7170
Size: 7507
BspHI T'CATG_A
Cuts at: 5396 7126 5396
Size: 1730 5777
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2187 3176 4390 4751 4862 844
Size: 1343 989 1214 361 111 3489
Fragments arranged by size:
3489 1343 1214 989 361 111
BsrBI CCG'CTC
Cuts at: 1394 1823 2028 2470 5394 1394
Size: 429 205 442 2924 3507
Fragments arranged by size:
3507 2924 442 429 205
BsrDI GCAATG_nn'
Cuts at: 65 1248 3007 3691 4717 4774 6002 6176 65
Size: 1183 1759 684 1026 57 1228 174 1396
Fragments arranged by size:
1759 1396 1228 1183 1026 684 174 57
BsrFI r'CCGG_y
Cuts at: 1700 1810 2870 4514 4619 6151 6338 1700
Size: 110 1060 1644 105 1532 187 2869
Fragments arranged by size:
2869 1644 1532 1060 187 110 105
BsrGI T'GTAC_A
Cuts at: 96 3075 96
Size: 2979 4528
BssHII G'CGCG_C
Cuts at: 1925 2478 2602 2756 1925
Size: 553 124 154 6676
Fragments arranged by size:
6676 553 154 124
BssSI C'ACGA_G
Cuts at: 5567 6579 5567
Size: 1012 6495
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4672 794
Size: 3878 3629
BstBI TT'CG_AA
Cuts at: 6311 6311
Size: 7507
BstEII G'GTnAC_C
Cuts at: 1184 3163 1184
Size: 1979 5528
BstXI CCAn_nnnn'nTGG
Cuts at: 2019 4348 4567 2019
Size: 2329 219 4959
Fragments arranged by size:
4959 2329 219
BstYI r'GATC_y
Cuts at: 1972 2112 2173 2812 3839 4837 5204 5589 5606
Size: 140 61 639 1027 998 367 385 17
Cuts at: 5606 7058 7144 7156 7502 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1027 998 639 385 367 346
140 86 61 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2094 2445 4293 4626 513
Size: 552 939 90 351 1848 333 3394
Fragments arranged by size:
3394 1848 939 552 351 333 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3100 3639 4521 487
Size: 683 56 1874 539 882 3473
Fragments arranged by size:
3473 1874 882 683 539 56
BtsI GCAGTG_nn'
Cuts at: 2303 3978 5023 5817 5837 2303
Size: 1675 1045 794 20 3973
Fragments arranged by size:
3973 1675 1045 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7507
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7472
DraI TTT'AAA
Cuts at: 4811 4811
Size: 7507
DraIII CAC_nnn'GTG
Cuts at: 3772 3772
Size: 7507
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6514 809
Size: 507 5198 1802
Fragments arranged by size:
5198 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3716 3763 4350
Size: 54 1425 50 275 18 1886 47 587
Cuts at: 4350 4954 5843 7472 8
Size: 604 889 1629 43
Fragments arranged by size:
1886 1629 1425 889 604 587 275 54
50 47 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4954 1537
Size: 275 3142 4090
Fragments arranged by size:
4090 3142 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5437 1353
Size: 4084 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2668 4564 6094 6468 6614 387
Size: 1064 138 1079 1896 1530 374 146 1280
Fragments arranged by size:
1896 1530 1280 1079 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3729 4200 4599 3729
Size: 471 399 6637
Fragments arranged by size:
6637 471 399
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2041 2235 2348 2372 2618 2786 4038
Size: 393 409 194 113 24 246 168 1252
Cuts at: 4038 4343 5570 6167 6954 7222 1239
Size: 305 1227 597 787 268 1524
Fragments arranged by size:
1524 1252 1227 787 597 409 393 305
268 246 194 168 113 24
EcoNI CCTnn'n_nnAGG
Cuts at: 3717 3717
Size: 7507
EcoO109I rG'GnC_Cy
Cuts at: 2664 2825 3451 3703 4220 4221 4753 2664
Size: 161 626 252 517 1 532 5418
Fragments arranged by size:
5418 626 532 517 252 161 1
EcoRI G'AATT_C
Cuts at: 4816 5194 4816
Size: 378 7129
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2523 3238 2523
Size: 715 6792
FspI TGC'GCA
Cuts at: 6013 6013
Size: 7507
HaeII r_GCGC'y
Cuts at: 1678 2991 4422 4641 6654 1678
Size: 1313 1431 219 2013 2531
Fragments arranged by size:
2531 2013 1431 1313 219
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2656 2688 2762 2794 3730
Size: 32 135 32 1084 32 74 32 936
Cuts at: 3730 3762 4366 4398 4583 4615 6148 6180 6222
Size: 32 604 32 185 32 1533 32 42
Cuts at: 6222 6254 1373
Size: 32 2626
Fragments arranged by size:
2626 1533 1084 936 604 185 135 74
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4851 5097 669
Size: 988 281 2913 246 3079
Fragments arranged by size:
3079 2913 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7507
HpaI GTT'AAC
Cuts at: 5097 5097
Size: 7507
KasI G'GCGC_C
Cuts at: 2987 2987
Size: 7507
KpnI G_GTAC'C
Cuts at: 3756 4832 3756
Size: 1076 6431
MfeI C'AATT_G
Cuts at: 5106 5106
Size: 7507
MluI A'CGCG_T
Cuts at: 7383 7383
Size: 7507
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2577 2634 3372 6621 6805 1305
Size: 1272 57 738 3249 184 2007
Fragments arranged by size:
3249 2007 1272 738 184 57
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3765 4352 10
Size: 54 1425 343 1933 587 3165
Fragments arranged by size:
3165 1933 1425 587 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2702 2789 3071 3659 4547 5465 5824
Size: 648 1536 87 282 588 888 918 359
Cuts at: 5824 5983 518
Size: 159 2042
Fragments arranged by size:
2042 1536 918 888 648 588 359 282
159 87
MspA1I CmG'CkG
Cuts at: 1606 2350 3761 4318 4491 4733 4923 5600 6748
Size: 744 1411 557 173 242 190 677 1148
Cuts at: 6748 6993 1606
Size: 245 2120
Fragments arranged by size:
2120 1411 1148 744 677 557 245 242
190 173
NaeI GCC'GGC
Cuts at: 2872 4621 2872
Size: 1749 5758
NarI GG'CG_CC
Cuts at: 2988 2988
Size: 7507
NcoI C'CATG_G
Cuts at: 513 1065 2004 4626 513
Size: 552 939 2622 3394
Fragments arranged by size:
3394 2622 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7507
NgoMIV G'CCGG_C
Cuts at: 2870 4619 2870
Size: 1749 5758
NheI G'CTAG_C
Cuts at: 1052 2219 1052
Size: 1167 6340
NotI GC'GGCC_GC
Cuts at: 4954 4954
Size: 7507
NruI TCG'CGA
Cuts at: 2159 2159
Size: 7507
NspI r_CATG'y
Cuts at: 1132 1229 4865 6410 1132
Size: 97 3636 1545 2229
Fragments arranged by size:
3636 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 4606 1348
Size: 3258 4249
PciI A'CATG_T
Cuts at: 6406 6406
Size: 7507
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4763 1108
Size: 3655 3852
PfoI T'CCnGG_A
Cuts at: 2115 2379 2763 4426 2115
Size: 264 384 1663 5196
Fragments arranged by size:
5196 1663 384 264
PmeI GTTT'AAAC
Cuts at: 4811 4811
Size: 7507
PmlI CAC'GTG
Cuts at: 4672 4672
Size: 7507
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5576 5608 7089 7121 5576
Size: 32 1481 32 5962
Fragments arranged by size:
5962 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2664 3451 3703 2664
Size: 787 252 6468
Fragments arranged by size:
6468 787 252
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7507
PsiI TTA'TAA
Cuts at: 5077 5077
Size: 7507
PspOMI G'GGCC_C
Cuts at: 4220 4220
Size: 7507
PspXI vC'TCGA_Gb
Cuts at: 3747 3747
Size: 7507
PstI C_TGCA'G
Cuts at: 830 4668 4859 830
Size: 3838 191 3478
Fragments arranged by size:
3838 3478 191
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7507
PvuII CAG'CTG
Cuts at: 3761 4733 3761
Size: 972 6535
SacI G_AGCT'C
Cuts at: 3731 4202 4601 3731
Size: 471 399 6637
Fragments arranged by size:
6637 471 399
SalI G'TCGA_C
Cuts at: 1936 4849 1936
Size: 2913 4594
SanDI GG'GwC_CC
Cuts at: 2664 3451 2664
Size: 787 6720
SbfI CC_TGCA'GG
Cuts at: 4859 4859
Size: 7507
ScaI AGT'ACT
Cuts at: 3892 5755 7467 3892
Size: 1863 1712 3932
Fragments arranged by size:
3932 1863 1712
SexAI A'CCwGG_T
Cuts at: 4330 4330
Size: 7507
SfcI C'TryA_G
Cuts at: 826 1045 3826 4393 4664 4855 5990 6671 6862
Size: 219 2781 567 271 191 1135 681 191
Cuts at: 6862 826
Size: 1471
Fragments arranged by size:
2781 1471 1135 681 567 271 219 191
191
SfoI GGC'GCC
Cuts at: 2989 2989
Size: 7507
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7507
SgrAI Cr'CCGG_yG
Cuts at: 1700 2870 1700
Size: 1170 6337
SmaI CCC'GGG
Cuts at: 1872 3421 4055 4834 1872
Size: 1549 634 779 4545
Fragments arranged by size:
4545 1549 779 634
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2471 3212 3747 5611 6512 6774
Size: 197 926 528 741 535 1864 901 262
Cuts at: 6774 820
Size: 1553
Fragments arranged by size:
1864 1553 926 901 741 535 528 262
197
SnaBI TAC'GTA
Cuts at: 493 5298 493
Size: 4805 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7507
SphI G_CATG'C
Cuts at: 1132 4865 1132
Size: 3733 3774
SspI AAT'ATT
Cuts at: 5 52 5431 5
Size: 47 5379 2081
Fragments arranged by size:
5379 2081 47
StuI AGG'CCT
Cuts at: 1517 2018 4254 1517
Size: 501 2236 4770
Fragments arranged by size:
4770 2236 501
StyI C'CwwG_G
Cuts at: 513 1065 2004 2164 4626 4946 513
Size: 552 939 160 2462 320 3074
Fragments arranged by size:
3074 2462 939 552 320 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2628 3937 4397 5534 5551 5704 5889
Size: 1255 1048 1309 460 1137 17 153 185
Cuts at: 5889 6334 325
Size: 445 1498
Fragments arranged by size:
1498 1309 1255 1137 1048 460 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3075 3890 5753
Size: 274 80 33 51 1443 1098 815 1863
Cuts at: 5753 7465 96
Size: 1712 138
Fragments arranged by size:
1863 1712 1443 1098 815 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3171 3203 7467 7499 3171
Size: 32 4264 32 3179
Fragments arranged by size:
4264 3179 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7507
XbaI T'CTAG_A
Cuts at: 4172 4843 4172
Size: 671 6836
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3244 3244
Size: 7507
XhoI C'TCGA_G
Cuts at: 1943 2471 3747 1943
Size: 528 1276 5703
Fragments arranged by size:
5703 1276 528
XmaI C'CCGG_G
Cuts at: 1870 3419 4053 4832 1870
Size: 1549 634 779 4545
Fragments arranged by size:
4545 1549 779 634
XmnI GAAnn'nnTTC
Cuts at: 2135 3221 3307 5636 2135
Size: 1086 86 2329 4006
Fragments arranged by size:
4006 2329 1086 86
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5315 276
Size: 53 83 186 936 3781 2468
Fragments arranged by size:
3781 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SalI SanDI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I
EcoRV FseI FspAI NsiI PacI PsrI RsrII SacII
SapI SfiI SrfI SwaI