Restriction map for
pFN21AE6709
(Circular) (Six-base) MAPSORT of: pFN21AE6709.seq Check: 6432 from: 1 to: 6844
pFN21AE6709 6844 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4654 278
Size: 53 83 186 936 3118 2468
Fragments arranged by size:
3118 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4187 1937
Size: 2250 4594
Acc65I G'GTAC_C
Cuts at: 4165 4165
Size: 6844
AclI AA'CG_TT
Cuts at: 1563 4971 5344 1563
Size: 3408 373 3063
Fragments arranged by size:
3408 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3303 3713 4040 4161 4907 6291 6559 1632
Size: 1671 410 327 121 746 1384 268 1917
Fragments arranged by size:
1917 1671 1384 746 410 327 268 121
AfeI AGC'GCT
Cuts at: 3989 3989
Size: 6844
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6647
AflIII A'CryG_T
Cuts at: 1203 3538 5743 6720 1203
Size: 2335 2205 977 1327
Fragments arranged by size:
2335 2205 1327 977
AgeI A'CCGG_T
Cuts at: 5675 5675
Size: 6844
AhdI GACnn_n'nnGTC
Cuts at: 5573 5573
Size: 6844
AleI CACnn'nnGTG
Cuts at: 1166 2468 1166
Size: 1302 5542
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4019 1184
Size: 2835 4009
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2662 2694 2662
Size: 32 6812
AlwNI CAG_nnn'CTG
Cuts at: 2951 3661 4587 6159 2951
Size: 710 926 1572 3636
Fragments arranged by size:
3636 1572 926 710
ApaI G_GGCC'C
Cuts at: 2104 2310 2104
Size: 206 6638
ApaLI G'TGCA_C
Cuts at: 4901 6057 4901
Size: 1156 5688
ApoI r'AATT_y
Cuts at: 1493 2811 3052 3784 3821 4153 4379 4531 6640
Size: 1318 241 732 37 332 226 152 2109
Cuts at: 6640 1493
Size: 1697
Fragments arranged by size:
2109 1697 1318 732 332 241 226 152
37
AseI AT'TA_AT
Cuts at: 160 1032 5398 160
Size: 872 4366 1606
Fragments arranged by size:
4366 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6844
AvaI C'yCGr_G
Cuts at: 1870 1943 4169 1870
Size: 73 2226 4545
Fragments arranged by size:
4545 2226 73
BamHI G'GATC_C
Cuts at: 2094 3961 4174 4541 2094
Size: 1867 213 367 4397
Fragments arranged by size:
4397 1867 367 213
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2135 2441 3555 4165 5620
Size: 325 217 627 348 306 1114 610 1455
Cuts at: 5620 618
Size: 1842
Fragments arranged by size:
1842 1455 1114 627 610 348 325 306
217
BanII G_rGCy'C
Cuts at: 1391 2104 2310 2337 2920 3126 4032 4163 1391
Size: 713 206 27 583 206 906 131 4072
Fragments arranged by size:
4072 906 713 583 206 206 131 27
BbeI G_GCGC'C
Cuts at: 2139 2139
Size: 6844
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5873
BbvCI CC'TCA_GC
Cuts at: 3573 3573
Size: 6844
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5035 5069 1903
Size: 34 50 34 3014 34 3678
Fragments arranged by size:
3678 3014 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4736 5946 4736
Size: 1210 5634
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6844
BfrBI ATG'CAT
Cuts at: 3162 3508 3162
Size: 346 6498
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2070 2366 5455
Size: 107 122 71 631 605 398 296 3089
Cuts at: 5455 136
Size: 1525
Fragments arranged by size:
3089 1525 631 605 398 296 122 107
71
BglII A'GATC_T
Cuts at: 2872 6839 2872
Size: 3967 2877
BlpI GC'TnA_GC
Cuts at: 2861 4261 2861
Size: 1400 5444
Bme1580I G_kGCm'C
Cuts at: 1790 2104 2224 2310 3560 4093 4905 6061 1790
Size: 314 120 86 1250 533 812 1156 2573
Fragments arranged by size:
2573 1250 1156 812 533 314 120 86
BmgBI CAC'GTC
Cuts at: 1318 3541 1318
Size: 2223 4621
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3395 3555 4575
Size: 416 53 83 399 24 1978 160 1020
Cuts at: 4575 5533 442
Size: 958 1753
Fragments arranged by size:
1978 1753 1020 958 416 399 160 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6844
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5504 1239
Size: 4265 2579
Bpu10I CC'TnA_GC
Cuts at: 2211 2394 3573 2211
Size: 183 1179 5482
Fragments arranged by size:
5482 1179 183
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2278 3421 3788 4969 5834 6132 2278
Size: 1143 367 1181 865 298 2990
Fragments arranged by size:
2990 1181 1143 865 367 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2130 2768 5507 882
Size: 617 631 638 2739 2219
Fragments arranged by size:
2739 2219 638 631 617
BsaAI yAC'GTr
Cuts at: 493 1204 4635 493
Size: 711 3431 2702
Fragments arranged by size:
3431 2702 711
BsaBI GATnn'nnATC
Cuts at: 2478 6838 2478
Size: 4360 2484
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2136 4651 5033 5668
Size: 53 83 186 936 603 2515 382 635
Cuts at: 5668 275
Size: 1451
Fragments arranged by size:
2515 1451 936 635 603 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5277 5675 5949 6096 6507 5277
Size: 398 274 147 411 5614
Fragments arranged by size:
5614 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6619
Fragments arranged by size:
6619 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6844
BseYI C'CCAG_C
Cuts at: 2023 2738 3125 6047 6543 2023
Size: 715 387 2922 496 2324
Fragments arranged by size:
2922 2324 715 496 387
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3123 1163
Size: 573 1387 4884
Fragments arranged by size:
4884 1387 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3927 4294 5055 6083 1540
Size: 275 186 1926 367 761 1028 2301
Fragments arranged by size:
2301 1926 1028 761 367 275 186
BsiHKAI G_wGCw'C
Cuts at: 2337 4905 4990 6061 2337
Size: 2568 85 1071 3120
Fragments arranged by size:
3120 2568 1071 85
BsmI GAATG_Cn'
Cuts at: 2402 3508 4355 4448 2402
Size: 1106 847 93 4798
Fragments arranged by size:
4798 1106 847 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2104 2224 2310 2337 2920 3126 3560
Size: 399 314 120 86 27 583 206 434
Cuts at: 3560 4032 4093 4163 4905 4990 6061 1391
Size: 472 61 70 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 583 472 434 399 314
206 120 86 85 70 61 27
BspEI T'CCGG_A
Cuts at: 6507 6507
Size: 6844
BspHI T'CATG_A
Cuts at: 3274 4733 6463 3274
Size: 1459 1730 3655
Fragments arranged by size:
3655 1730 1459
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4199 844
Size: 3355 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3923 4731 1394
Size: 429 2100 808 3507
Fragments arranged by size:
3507 2100 808 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5339 5513 65
Size: 1183 4091 174 1396
Fragments arranged by size:
4091 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5488 5675 1700
Size: 110 3678 187 2869
Fragments arranged by size:
3678 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6844
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6844
BssSI C'ACGA_G
Cuts at: 3878 4904 5916 3878
Size: 1026 1012 4806
Fragments arranged by size:
4806 1026 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3514 794
Size: 2720 4124
BstBI TT'CG_AA
Cuts at: 4119 5648 4119
Size: 1529 5315
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6844
BstXI CCAn_nnnn'nTGG
Cuts at: 4039 4039
Size: 6844
BstYI r'GATC_y
Cuts at: 1972 2094 2872 3961 4108 4174 4541 4926 4943
Size: 122 778 1089 147 66 367 385 17
Cuts at: 4943 6395 6481 6493 6839 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1089 778 385 367 346 147
122 86 66 17 12
Bsu36I CC'TnA_GG
Cuts at: 3779 3779
Size: 6844
BtgI C'CryG_G
Cuts at: 513 1065 2130 2445 3361 3763 3933 4012 513
Size: 552 1065 315 916 402 170 79 3345
Fragments arranged by size:
3345 1065 916 552 402 315 170 79
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6105
Fragments arranged by size:
6105 683 56
BtsI GCAGTG_nn'
Cuts at: 2625 3659 4360 5154 5174 2625
Size: 1034 701 794 20 4295
Fragments arranged by size:
4295 1034 794 701 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6844
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2529 2564 2871 2906 529
Size: 35 1965 35 307 35 4467
Fragments arranged by size:
4467 1965 307 35 35 35
DraI TTT'AAA
Cuts at: 4148 4148
Size: 6844
DraIII CAC_nnn'GTG
Cuts at: 3880 3880
Size: 6844
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5851 809
Size: 507 4535 1802
Fragments arranged by size:
4535 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2358 4291 5180
Size: 54 1425 50 275 18 528 1933 889
Cuts at: 5180 6809 8
Size: 1629 43
Fragments arranged by size:
1933 1629 1425 889 528 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4291 1537
Size: 275 2479 4090
Fragments arranged by size:
4090 2479 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4774 1353
Size: 3421 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5431 5805 5951 387
Size: 1064 138 3842 374 146 1280
Fragments arranged by size:
3842 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2335 2335
Size: 6844
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3303 3713 4040 4161 4907 5504 6291
Size: 393 1671 410 327 121 746 597 787
Cuts at: 6291 6559 1239
Size: 268 1524
Fragments arranged by size:
1671 1524 787 746 597 410 393 327
268 121
EcoNI CCTnn'n_nnAGG
Cuts at: 3228 3228
Size: 6844
EcoO109I rG'GnC_Cy
Cuts at: 2307 3073 2307
Size: 766 6078
EcoRI G'AATT_C
Cuts at: 3784 4153 4531 3784
Size: 369 378 6097
Fragments arranged by size:
6097 378 369
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2719 4070 2719
Size: 1351 5493
FspI TGC'GCA
Cuts at: 5350 5350
Size: 6844
HaeII r_GCGC'y
Cuts at: 1678 2139 2191 3991 5991 1678
Size: 461 52 1800 2000 2531
Fragments arranged by size:
2531 2000 1800 461 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2321 2353 3463 3495 5485
Size: 32 135 32 749 32 1110 32 1990
Cuts at: 5485 5517 5559 5591 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1990 1110 749 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3625 4188 4434 669
Size: 988 281 1687 563 246 3079
Fragments arranged by size:
3079 1687 988 563 281 246
HindIII A'AGCT_T
Cuts at: 748 2424 748
Size: 1676 5168
HpaI GTT'AAC
Cuts at: 3625 4434 3625
Size: 809 6035
KasI G'GCGC_C
Cuts at: 2135 2135
Size: 6844
KpnI G_GTAC'C
Cuts at: 4169 4169
Size: 6844
MfeI C'AATT_G
Cuts at: 2837 4443 2837
Size: 1606 5238
MluI A'CGCG_T
Cuts at: 6720 6720
Size: 6844
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1996 3473 4125 5958 6142 1305
Size: 691 1477 652 1833 184 2007
Fragments arranged by size:
2007 1833 1477 691 652 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5022
Fragments arranged by size:
5022 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2468 4037 4802 5161 5320 518
Size: 648 1302 1569 765 359 159 2042
Fragments arranged by size:
2042 1569 1302 765 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2121 3716 3862 4260 4937 6085 6330 1606
Size: 515 1595 146 398 677 1148 245 2120
Fragments arranged by size:
2120 1595 1148 677 515 398 245 146
NarI GG'CG_CC
Cuts at: 2136 2136
Size: 6844
NcoI C'CATG_G
Cuts at: 513 1065 2445 3763 3933 513
Size: 552 1380 1318 170 3424
Fragments arranged by size:
3424 1380 1318 552 170
NdeI CA'TA_TG
Cuts at: 387 3838 387
Size: 3451 3393
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6844
NotI GC'GGCC_GC
Cuts at: 4291 4291
Size: 6844
NsiI A_TGCA'T
Cuts at: 3164 3510 3164
Size: 346 6498
NspI r_CATG'y
Cuts at: 1132 1229 2292 3162 3503 4202 5747 1132
Size: 97 1063 870 341 699 1545 2229
Fragments arranged by size:
2229 1545 1063 870 699 341 97
PasI CC'CwG_GG
Cuts at: 1348 2158 3069 1348
Size: 810 911 5123
Fragments arranged by size:
5123 911 810
PciI A'CATG_T
Cuts at: 5743 5743
Size: 6844
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3493 3880 3939 1108
Size: 2385 387 59 4013
Fragments arranged by size:
4013 2385 387 59
PmeI GTTT'AAAC
Cuts at: 4148 4148
Size: 6844
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4913 4945 6426 6458 4913
Size: 32 1481 32 5299
Fragments arranged by size:
5299 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3073 3073
Size: 6844
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6844
PsiI TTA'TAA
Cuts at: 2808 4414 2808
Size: 1606 5238
PspOMI G'GGCC_C
Cuts at: 2100 2306 2100
Size: 206 6638
PstI C_TGCA'G
Cuts at: 830 3715 4196 830
Size: 2885 481 3478
Fragments arranged by size:
3478 2885 481
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6844
PvuII CAG'CTG
Cuts at: 2121 3716 3862 2121
Size: 1595 146 5103
Fragments arranged by size:
5103 1595 146
SacI G_AGCT'C
Cuts at: 2337 2337
Size: 6844
SalI G'TCGA_C
Cuts at: 1936 4186 1936
Size: 2250 4594
SanDI GG'GwC_CC
Cuts at: 3073 3073
Size: 6844
SbfI CC_TGCA'GG
Cuts at: 4196 4196
Size: 6844
ScaI AGT'ACT
Cuts at: 3599 5092 6804 3599
Size: 1493 1712 3639
Fragments arranged by size:
3639 1712 1493
SexAI A'CCwGG_T
Cuts at: 3551 3551
Size: 6844
SfcI C'TryA_G
Cuts at: 826 1045 3085 3711 3956 4192 5327 6008 6199
Size: 219 2040 626 245 236 1135 681 191
Cuts at: 6199 826
Size: 1471
Fragments arranged by size:
2040 1471 1135 681 626 245 236 219
191
SfoI GGC'GCC
Cuts at: 2137 2137
Size: 6844
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6844
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6844
SmaI CCC'GGG
Cuts at: 1872 4171 1872
Size: 2299 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2257 3436 3803 4948 5849 6111
Size: 197 926 314 1179 367 1145 901 262
Cuts at: 6111 820
Size: 1553
Fragments arranged by size:
1553 1179 1145 926 901 367 314 262
197
SnaBI TAC'GTA
Cuts at: 493 4635 493
Size: 4142 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6844
SphI G_CATG'C
Cuts at: 1132 3162 4202 1132
Size: 2030 1040 3774
Fragments arranged by size:
3774 2030 1040
SspI AAT'ATT
Cuts at: 5 52 3118 3178 4768 5
Size: 47 3066 60 1590 2081
Fragments arranged by size:
3066 2081 1590 60 47
StuI AGG'CCT
Cuts at: 1517 3171 1517
Size: 1654 5190
StyI C'CwwG_G
Cuts at: 513 1065 2445 2923 3763 3933 4283 513
Size: 552 1380 478 840 170 350 3074
Fragments arranged by size:
3074 1380 840 552 478 350 170
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4871 4888 5041 5226 5671 325
Size: 1255 3291 17 153 185 445 1498
Fragments arranged by size:
3291 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2654 3597 5090
Size: 274 80 33 51 1443 677 943 1493
Cuts at: 5090 6802 96
Size: 1712 138
Fragments arranged by size:
1712 1493 1443 943 677 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6804 6836 6804
Size: 32 6812
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6844
XbaI T'CTAG_A
Cuts at: 2593 4180 2593
Size: 1587 5257
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3542 3852 3542
Size: 310 6534
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6844
XmaI C'CCGG_G
Cuts at: 1870 4169 1870
Size: 2299 4545
XmnI GAAnn'nnTTC
Cuts at: 2232 2715 4973 2232
Size: 483 2258 4103
Fragments arranged by size:
4103 2258 483
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4652 276
Size: 53 83 186 936 3118 2468
Fragments arranged by size:
3118 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BplI BsiWI BsmBI BstZ17I
EcoRV FseI FspAI NaeI NgoMIV NruI PacI PfoI
PmlI PspXI PsrI RsrII SacII SapI SfiI SrfI
SwaI