Restriction map for
pFN21AE6708
(Circular) (Six-base) MAPSORT of: pFN21AE6708.seq Check: 2368 from: 1 to: 6922
pFN21AE6708 6922 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3228 3370 3228
Size: 142 6780
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4732 278
Size: 53 83 186 936 3196 2468
Fragments arranged by size:
3196 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4265 1937
Size: 2328 4594
Acc65I G'GTAC_C
Cuts at: 4243 4243
Size: 6922
AclI AA'CG_TT
Cuts at: 1563 5049 5422 1563
Size: 3486 373 3063
Fragments arranged by size:
3486 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2507 2774 3218 3740 4985 6369 6637 1632
Size: 875 267 444 522 1245 1384 268 1917
Fragments arranged by size:
1917 1384 1245 875 522 444 268 267
AfeI AGC'GCT
Cuts at: 3467 3467
Size: 6922
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6725
AflIII A'CryG_T
Cuts at: 1203 2706 3499 5821 6798 1203
Size: 1503 793 2322 977 1327
Fragments arranged by size:
2322 1503 1327 977 793
AgeI A'CCGG_T
Cuts at: 5753 5753
Size: 6922
AhdI GACnn_n'nnGTC
Cuts at: 5651 5651
Size: 6922
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6922
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6922
AlwNI CAG_nnn'CTG
Cuts at: 3208 4665 6237 3208
Size: 1457 1572 3893
Fragments arranged by size:
3893 1572 1457
ApaI G_GGCC'C
Cuts at: 3163 3163
Size: 6922
ApaLI G'TGCA_C
Cuts at: 4979 6135 4979
Size: 1156 5766
ApoI r'AATT_y
Cuts at: 1493 4231 4457 4609 6718 1493
Size: 2738 226 152 2109 1697
Fragments arranged by size:
2738 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5476 160
Size: 872 4444 1606
Fragments arranged by size:
4444 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6922
AvaI C'yCGr_G
Cuts at: 1870 1943 2234 3258 4247 1870
Size: 73 291 1024 989 4545
Fragments arranged by size:
4545 1024 989 291 73
BamHI G'GATC_C
Cuts at: 2098 4252 4619 2098
Size: 2154 367 4401
Fragments arranged by size:
4401 2154 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2041 2632 2656 3020 4243
Size: 325 217 627 254 591 24 364 1223
Cuts at: 4243 5698 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1223 627 591 364 325 254
217 24
BanII G_rGCy'C
Cuts at: 1391 2227 3163 3495 3963 4241 1391
Size: 836 936 332 468 278 4072
Fragments arranged by size:
4072 936 836 468 332 278
BbeI G_GCGC'C
Cuts at: 2045 2045
Size: 6922
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3183 928
Size: 971 1284 4667
Fragments arranged by size:
4667 1284 971
BbvCI CC'TCA_GC
Cuts at: 3093 3647 3093
Size: 554 6368
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5113 5147 1903
Size: 34 3176 34 3678
Fragments arranged by size:
3678 3176 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4814 6024 4814
Size: 1210 5712
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6922
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5533 136
Size: 107 122 71 631 605 3861 1525
Fragments arranged by size:
3861 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6917 6917
Size: 6922
BlpI GC'TnA_GC
Cuts at: 3957 4339 3957
Size: 382 6540
Bme1580I G_kGCm'C
Cuts at: 1790 3148 3163 4983 6139 1790
Size: 1358 15 1820 1156 2573
Fragments arranged by size:
2573 1820 1358 1156 15
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6922
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4653 5611 442
Size: 416 53 83 399 24 3236 958 1753
Fragments arranged by size:
3236 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6922
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2182 2426 2780 2798 3023 3273 3422 3620
Size: 943 244 354 18 225 250 149 198
Cuts at: 3620 3731 3902 5582 1239
Size: 111 171 1680 2579
Fragments arranged by size:
2579 1680 943 354 250 244 225 198
171 149 111 18
Bpu10I CC'TnA_GC
Cuts at: 3093 3647 3093
Size: 554 6368
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2360 2723 3287 5047 5912 6210 2360
Size: 363 564 1760 865 298 3072
Fragments arranged by size:
3072 1760 865 564 363 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3566 5585 882
Size: 617 2067 2019 2219
Fragments arranged by size:
2219 2067 2019 617
BsaAI yAC'GTr
Cuts at: 493 1204 3799 4713 493
Size: 711 2595 914 2702
Fragments arranged by size:
2702 2595 914 711
BsaBI GATnn'nnATC
Cuts at: 6916 6916
Size: 6922
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2042 3168 4729
Size: 53 83 186 936 476 33 1126 1561
Cuts at: 4729 5111 5746 275
Size: 382 635 1451
Fragments arranged by size:
1561 1451 1126 936 635 476 382 186
83 53 33
BsaWI w'CCGG_w
Cuts at: 2604 5355 5753 6027 6174 6585 2604
Size: 2751 398 274 147 411 2941
Fragments arranged by size:
2941 2751 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2180 2210 2996 3026 3271
Size: 30 165 30 777 30 786 30 245
Cuts at: 3271 3301 1178
Size: 30 4799
Fragments arranged by size:
4799 786 777 245 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2038 2759 2825 3434 3719 4169 4181 1186
Size: 852 721 66 609 285 450 12 3927
Fragments arranged by size:
3927 852 721 609 450 285 66 12
BseYI C'CCAG_C
Cuts at: 2903 6125 6621 2903
Size: 3222 496 3204
Fragments arranged by size:
3222 3204 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2152 3200 3752 3860 1163
Size: 573 416 1048 552 108 4225
Fragments arranged by size:
4225 1048 573 552 416 108
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2038 2975 4372 5133 6161 1540
Size: 275 186 37 937 1397 761 1028 2301
Fragments arranged by size:
2301 1397 1028 937 761 275 186 37
BsiHKAI G_wGCw'C
Cuts at: 3444 3963 4983 5068 6139 3444
Size: 519 1020 85 1071 4227
Fragments arranged by size:
4227 1071 1020 519 85
BsmI GAATG_Cn'
Cuts at: 4433 4526 4433
Size: 93 6829
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2227 3148 3163 3444 3495 3963 4241
Size: 399 437 921 15 281 51 468 278
Cuts at: 4241 4983 5068 6139 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 921 742 468 437 399 281
278 85 51 15
BspEI T'CCGG_A
Cuts at: 2604 6585 2604
Size: 3981 2941
BspHI T'CATG_A
Cuts at: 4811 6541 4811
Size: 1730 5192
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3222 3228 3370 4277 844
Size: 2378 6 142 907 3489
Fragments arranged by size:
3489 2378 907 142 6
BsrBI CCG'CTC
Cuts at: 1394 1823 4809 1394
Size: 429 2986 3507
Fragments arranged by size:
3507 2986 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2914 5417 5591 65
Size: 1183 1666 2503 174 1396
Fragments arranged by size:
2503 1666 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5566 5753 1700
Size: 110 3756 187 2869
Fragments arranged by size:
3756 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2187 2424 96
Size: 2091 237 4594
Fragments arranged by size:
4594 2091 237
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6922
BssSI C'ACGA_G
Cuts at: 3147 4982 5994 3147
Size: 1835 1012 4075
Fragments arranged by size:
4075 1835 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2473 794
Size: 1679 5243
BstBI TT'CG_AA
Cuts at: 5726 5726
Size: 6922
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6922
BstXI CCAn_nnnn'nTGG
Cuts at: 2128 3899 4123 2128
Size: 1771 224 4927
Fragments arranged by size:
4927 1771 224
BstYI r'GATC_y
Cuts at: 1972 2098 2607 2880 3231 4252 4619 5004 5021
Size: 126 509 273 351 1021 367 385 17
Cuts at: 5021 6473 6559 6571 6917 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1021 509 385 367 351 346
273 126 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3185 3185
Size: 6922
BtgI C'CryG_G
Cuts at: 513 1065 2004 2032 2081 2264 2500 3930 513
Size: 552 939 28 49 183 236 1430 3505
Fragments arranged by size:
3505 1430 939 552 236 183 49 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2364 3225 3666 487
Size: 683 56 1138 861 441 3743
Fragments arranged by size:
3743 1138 861 683 441 56
BtsI GCAGTG_nn'
Cuts at: 3209 3311 3585 4438 5232 5252 3209
Size: 102 274 853 794 20 4879
Fragments arranged by size:
4879 853 794 274 102 20
ClaI AT'CG_AT
Cuts at: 1554 3861 1554
Size: 2307 4615
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6887
DraI TTT'AAA
Cuts at: 4226 4226
Size: 6922
DraIII CAC_nnn'GTG
Cuts at: 2185 2185
Size: 6922
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4067 5929 809
Size: 507 2751 1862 1802
Fragments arranged by size:
2751 1862 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2035 2566 2784
Size: 54 1425 50 275 18 205 531 218
Cuts at: 2784 2972 3360 3426 3915 4017 4369 5258 6887
Size: 188 388 66 489 102 352 889 1629
Cuts at: 6887 8
Size: 43
Fragments arranged by size:
1629 1425 889 531 489 388 352 275
218 205 188 102 66 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2035 2972 4369 1537
Size: 275 223 937 1397 4090
Fragments arranged by size:
4090 1397 937 275 223
EarI CTCTTCn'nnn_
Cuts at: 1353 2338 2627 2702 3612 4211 4852 1353
Size: 985 289 75 910 599 641 3423
Fragments arranged by size:
3423 985 910 641 599 289 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2498 2615 5509 5883 6029 387
Size: 1064 138 909 117 2894 374 146 1280
Fragments arranged by size:
2894 1280 1064 909 374 146 138 117
EcoICRI GAG'CTC
Cuts at: 3961 3961
Size: 6922
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2182 2426 2507 2774 2780 2798 3023
Size: 393 550 244 81 267 6 18 225
Cuts at: 3023 3218 3273 3422 3620 3731 3740 3902 4985
Size: 195 55 149 198 111 9 162 1083
Cuts at: 4985 5582 6369 6637 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1083 787 597 550 393 268 267
244 225 198 195 162 149 111 81
55 18 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 3224 4070 3224
Size: 846 6076
EcoO109I rG'GnC_Cy
Cuts at: 2300 3160 2300
Size: 860 6062
EcoRI G'AATT_C
Cuts at: 4231 4609 4231
Size: 378 6544
FspI TGC'GCA
Cuts at: 5428 5428
Size: 6922
HaeII r_GCGC'y
Cuts at: 1678 2045 2264 2850 3469 6069 1678
Size: 367 219 586 619 2600 2531
Fragments arranged by size:
2600 2531 619 586 367 219
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2717 2749 2996 3028 3269
Size: 32 135 32 1145 32 247 32 241
Cuts at: 3269 3301 3644 3676 5563 5595 5637 5669 1373
Size: 32 343 32 1887 32 42 32 2626
Fragments arranged by size:
2626 1887 1145 343 247 241 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4054 4266 4512 669
Size: 988 281 2116 212 246 3079
Fragments arranged by size:
3079 2116 988 281 246 212
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6922
HpaI GTT'AAC
Cuts at: 4512 4512
Size: 6922
KasI G'GCGC_C
Cuts at: 2041 2041
Size: 6922
KpnI G_GTAC'C
Cuts at: 4247 4247
Size: 6922
MfeI C'AATT_G
Cuts at: 4521 4521
Size: 6922
MluI A'CGCG_T
Cuts at: 6798 6798
Size: 6922
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6036 6220 1305
Size: 4731 184 2007
Fragments arranged by size:
4731 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2786 10
Size: 54 1425 343 954 4146
Fragments arranged by size:
4146 1425 954 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4880 5239 5398 518
Size: 648 3714 359 159 2042
Fragments arranged by size:
3714 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2034 2083 2115 2470 2980 3196 3880 4338
Size: 428 49 32 355 510 216 684 458
Cuts at: 4338 5015 6163 6408 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 684 677 510 458 428 355
245 216 49 32
NarI GG'CG_CC
Cuts at: 2042 2042
Size: 6922
NcoI C'CATG_G
Cuts at: 513 1065 2004 2264 2500 513
Size: 552 939 260 236 4935
Fragments arranged by size:
4935 939 552 260 236
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6922
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6922
NotI GC'GGCC_GC
Cuts at: 4369 4369
Size: 6922
NruI TCG'CGA
Cuts at: 2349 2349
Size: 6922
NspI r_CATG'y
Cuts at: 1132 1229 2548 2710 3157 3928 4280 5825 1132
Size: 97 1319 162 447 771 352 1545 2229
Fragments arranged by size:
2229 1545 1319 771 447 352 162 97
PasI CC'CwG_GG
Cuts at: 1348 2128 2461 3163 1348
Size: 780 333 702 5107
Fragments arranged by size:
5107 780 702 333
PciI A'CATG_T
Cuts at: 2706 5821 2706
Size: 3115 3807
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6922
PfoI T'CCnGG_A
Cuts at: 2796 2883 3273 2796
Size: 87 390 6445
Fragments arranged by size:
6445 390 87
PmeI GTTT'AAAC
Cuts at: 4226 4226
Size: 6922
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4991 5023 6504 6536 4991
Size: 32 1481 32 5377
Fragments arranged by size:
5377 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2300 2300
Size: 6922
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6922
PsiI TTA'TAA
Cuts at: 4492 4492
Size: 6922
PspOMI G'GGCC_C
Cuts at: 3159 3159
Size: 6922
PstI C_TGCA'G
Cuts at: 830 3132 3318 3417 3462 3567 3672 4274 830
Size: 2302 186 99 45 105 105 602 3478
Fragments arranged by size:
3478 2302 602 186 105 105 99 45
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6922
PvuII CAG'CTG
Cuts at: 2115 2980 3196 3880 2115
Size: 865 216 684 5157
Fragments arranged by size:
5157 865 684 216
SacI G_AGCT'C
Cuts at: 3963 3963
Size: 6922
SacII CC_GC'GG
Cuts at: 2035 2084 2035
Size: 49 6873
SalI G'TCGA_C
Cuts at: 1936 4264 1936
Size: 2328 4594
SapI GCTCTTCn'nnn_
Cuts at: 2702 4211 2702
Size: 1509 5413
SbfI CC_TGCA'GG
Cuts at: 4274 4274
Size: 6922
ScaI AGT'ACT
Cuts at: 5170 6882 5170
Size: 1712 5210
SfcI C'TryA_G
Cuts at: 826 1045 3008 3128 3314 3413 3458 3563 3668
Size: 219 1963 120 186 99 45 105 105
Cuts at: 3668 3739 4270 5405 6086 6277 826
Size: 71 531 1135 681 191 1471
Fragments arranged by size:
1963 1471 1135 681 531 219 191 186
120 105 105 99 71 45
SfoI GGC'GCC
Cuts at: 2043 2043
Size: 6922
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6922
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6922
SmaI CCC'GGG
Cuts at: 1872 3260 4249 1872
Size: 1388 989 4545
Fragments arranged by size:
4545 1388 989
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2375 2738 3302 5026 5927 6189
Size: 197 926 432 363 564 1724 901 262
Cuts at: 6189 820
Size: 1553
Fragments arranged by size:
1724 1553 926 901 564 432 363 262
197
SnaBI TAC'GTA
Cuts at: 493 4713 493
Size: 4220 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6922
SphI G_CATG'C
Cuts at: 1132 2548 3157 3928 4280 1132
Size: 1416 609 771 352 3774
Fragments arranged by size:
3774 1416 771 609 352
SspI AAT'ATT
Cuts at: 5 52 4846 5
Size: 47 4794 2081
Fragments arranged by size:
4794 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6922
StyI C'CwwG_G
Cuts at: 513 1065 2004 2264 2500 2838 3693 4361 513
Size: 552 939 260 236 338 855 668 3074
Fragments arranged by size:
3074 939 855 668 552 338 260 236
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3545 4949 4966 5119 5304 5749 325
Size: 1255 1965 1404 17 153 185 445 1498
Fragments arranged by size:
1965 1498 1404 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2187 2424 5168
Size: 274 80 33 51 1443 210 237 2744
Cuts at: 5168 6880 96
Size: 1712 138
Fragments arranged by size:
2744 1712 1443 274 237 210 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3271 3303 6882 6914 3271
Size: 32 3579 32 3279
Fragments arranged by size:
3579 3279 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2731 3557 4067 1316
Size: 1415 826 510 4171
Fragments arranged by size:
4171 1415 826 510
XbaI T'CTAG_A
Cuts at: 4258 4258
Size: 6922
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2812 4120 2812
Size: 1308 5614
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6922
XmaI C'CCGG_G
Cuts at: 1870 3258 4247 1870
Size: 1388 989 4545
Fragments arranged by size:
4545 1388 989
XmnI GAAnn'nnTTC
Cuts at: 2696 4101 5051 2696
Size: 1405 950 4567
Fragments arranged by size:
4567 1405 950
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4730 276
Size: 53 83 186 936 3196 2468
Fragments arranged by size:
3196 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NruI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV NsiI
PacI PmlI PspXI PsrI RsrII SanDI SexAI SfiI
SrfI SwaI