Restriction map for
pFN21AE6707
(Circular) (Six-base) MAPSORT of: pFN21AE6707.seq Check: 1402 from: 1 to: 8434
pFN21AE6707 8434 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2312 2312
Size: 8434
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4839 6244 278
Size: 53 83 186 936 3303 1405 2468
Fragments arranged by size:
3303 2468 1405 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4869 4986 5394 5777 1937
Size: 2932 117 408 383 4594
Fragments arranged by size:
4594 2932 408 383 117
Acc65I G'GTAC_C
Cuts at: 5755 5755
Size: 8434
AclI AA'CG_TT
Cuts at: 1563 6561 6934 1563
Size: 4998 373 3063
Fragments arranged by size:
4998 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3932 5059 5735 6497 7881 8149 1632
Size: 2300 1127 676 762 1384 268 1917
Fragments arranged by size:
2300 1917 1384 1127 762 676 268
AfeI AGC'GCT
Cuts at: 4493 4493
Size: 8434
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8237
AflIII A'CryG_T
Cuts at: 1203 7333 8310 1203
Size: 6130 977 1327
Fragments arranged by size:
6130 1327 977
AgeI A'CCGG_T
Cuts at: 7265 7265
Size: 8434
AhdI GACnn_n'nnGTC
Cuts at: 3520 7163 3520
Size: 3643 4791
AleI CACnn'nnGTG
Cuts at: 1166 4778 1166
Size: 3612 4822
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3033 5547 1184
Size: 1849 2514 4071
Fragments arranged by size:
4071 2514 1849
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4736 4768 4736
Size: 32 8402
AlwNI CAG_nnn'CTG
Cuts at: 3179 3332 3370 4593 4888 5163 5332 5476 6177
Size: 153 38 1223 295 275 169 144 701
Cuts at: 6177 7749 3179
Size: 1572 3864
Fragments arranged by size:
3864 1572 1223 701 295 275 169 153
144 38
ApaI G_GGCC'C
Cuts at: 2160 2559 2671 3217 4830 5639 5663 2160
Size: 399 112 546 1613 809 24 4931
Fragments arranged by size:
4931 1613 809 546 399 112 24
ApaLI G'TGCA_C
Cuts at: 6491 7647 6491
Size: 1156 7278
ApoI r'AATT_y
Cuts at: 1493 2321 5743 5969 6121 8230 1493
Size: 828 3422 226 152 2109 1697
Fragments arranged by size:
3422 2109 1697 828 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6988 160
Size: 872 5956 1606
Fragments arranged by size:
5956 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8434
AvaI C'yCGr_G
Cuts at: 1870 1943 2159 2816 3021 3926 4220 4535 4933
Size: 73 216 657 205 905 294 315 398
Cuts at: 4933 5416 5539 5548 5629 5662 5759 1870
Size: 483 123 9 81 33 97 4545
Fragments arranged by size:
4545 905 657 483 398 315 294 216
205 123 97 81 73 33 9
BamHI G'GATC_C
Cuts at: 4902 5764 6131 4902
Size: 862 367 7205
Fragments arranged by size:
7205 862 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2861 3442 3593 3659 3749
Size: 325 217 627 1074 581 151 66 90
Cuts at: 3749 4030 4372 4400 4913 5141 5755 7210 618
Size: 281 342 28 513 228 614 1455 1842
Fragments arranged by size:
1842 1455 1074 627 614 581 513 342
325 281 228 217 151 90 66 28
BanII G_rGCy'C
Cuts at: 1391 2160 2204 2559 2671 3217 3377 3497 3927
Size: 769 44 355 112 546 160 120 430
Cuts at: 3927 3935 3984 4227 4830 5522 5639 5663 5753
Size: 8 49 243 603 692 117 24 90
Cuts at: 5753 1391
Size: 4072
Fragments arranged by size:
4072 769 692 603 546 430 355 243
160 120 117 112 90 49 44 24
8
BbeI G_GCGC'C
Cuts at: 3597 5145 3597
Size: 1548 6886
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2371 3085 5065 928
Size: 971 472 714 1980 4297
Fragments arranged by size:
4297 1980 971 714 472
BbvCI CC'TCA_GC
Cuts at: 3267 3267
Size: 8434
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5223 5257 6625 6659 1903
Size: 34 50 34 3202 34 1368 34 3678
Fragments arranged by size:
3678 3202 1368 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6326 7536 6326
Size: 1210 7224
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8434
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3386 4148 7045
Size: 107 122 71 631 605 1714 762 2897
Cuts at: 7045 136
Size: 1525
Fragments arranged by size:
2897 1714 1525 762 631 605 122 107
71
BglII A'GATC_T
Cuts at: 4176 8429 4176
Size: 4253 4181
BlpI GC'TnA_GC
Cuts at: 2711 3371 4805 5851 2711
Size: 660 1434 1046 5294
Fragments arranged by size:
5294 1434 1046 660
Bme1580I G_kGCm'C
Cuts at: 1790 2160 2230 2536 2559 2671 2734 3217 3447
Size: 370 70 306 23 112 63 483 230
Cuts at: 3447 3752 4272 4377 4830 5639 5663 6495 7651
Size: 305 520 105 453 809 24 832 1156
Cuts at: 7651 1790
Size: 2573
Fragments arranged by size:
2573 1156 832 809 520 483 453 370
306 305 230 112 105 70 63 24
23
BmgBI CAC'GTC
Cuts at: 1318 4609 5152 1318
Size: 3291 543 4600
Fragments arranged by size:
4600 3291 543
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2539 2611 3146
Size: 416 53 83 399 24 1122 72 535
Cuts at: 3146 3812 4112 4603 4946 6165 7123 442
Size: 666 300 491 343 1219 958 1753
Fragments arranged by size:
1753 1219 1122 958 666 535 491 416
399 343 300 83 72 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8434
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2341 3055 3817 2341
Size: 714 762 6958
Fragments arranged by size:
6958 762 714
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3201 3354 4166 4324 4373 4382 4508 4872
Size: 1962 153 812 158 49 9 126 364
Cuts at: 4872 7094 1239
Size: 2222 2579
Fragments arranged by size:
2579 2222 1962 812 364 158 153 126
49 9
Bpu10I CC'TnA_GC
Cuts at: 2387 3267 3491 3921 2387
Size: 880 224 430 6900
Fragments arranged by size:
6900 880 430 224
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6559 7424 7722 6559
Size: 865 298 7271
Fragments arranged by size:
7271 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2333 4078 5379 7097 882
Size: 617 834 1745 1301 1718 2219
Fragments arranged by size:
2219 1745 1718 1301 834 617
BsaAI yAC'GTr
Cuts at: 493 1204 2197 3427 6225 493
Size: 711 993 1230 2798 2702
Fragments arranged by size:
2798 2702 1230 993 711
BsaBI GATnn'nnATC
Cuts at: 8428 8428
Size: 8434
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3339 3594 4836 5142
Size: 53 83 186 936 1806 255 1242 306
Cuts at: 5142 5265 6241 6623 7258 275
Size: 123 976 382 635 1451
Fragments arranged by size:
1806 1451 1242 976 936 635 382 306
255 186 123 83 53
BsaWI w'CCGG_w
Cuts at: 3805 4740 6867 7265 7539 7686 8097 3805
Size: 935 2127 398 274 147 411 4142
Fragments arranged by size:
4142 2127 935 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2186 2216 2961 2991 3329
Size: 30 165 30 783 30 745 30 338
Cuts at: 3329 3359 3684 3714 4481 4511 4845 4875 1178
Size: 30 325 30 767 30 334 30 4737
Fragments arranged by size:
4737 783 767 745 338 334 325 165
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2111 2416 2756 3101 3961 4370 4685 5527
Size: 925 305 340 345 860 409 315 842
Cuts at: 5527 5744 1186
Size: 217 3876
Fragments arranged by size:
3876 925 860 842 409 345 340 315
305 217
BseYI C'CCAG_C
Cuts at: 2064 2763 2808 3259 3891 3902 4043 4162 4443
Size: 699 45 451 632 11 141 119 281
Cuts at: 4443 4457 4972 5404 5639 7637 8133 2064
Size: 14 515 432 235 1998 496 2365
Fragments arranged by size:
2365 1998 699 632 515 496 451 432
281 235 141 119 45 14 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3287 3383 5357 1163
Size: 573 1551 96 1974 4240
Fragments arranged by size:
4240 1974 1551 573 96
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4200 5207 5884 6645 7673 1540
Size: 275 186 2199 1007 677 761 1028 2301
Fragments arranged by size:
2301 2199 1028 1007 761 677 275 186
BsiHKAI G_wGCw'C
Cuts at: 2831 3377 6495 6580 7651 2831
Size: 546 3118 85 1071 3614
Fragments arranged by size:
3614 3118 1071 546 85
BsmI GAATG_Cn'
Cuts at: 2278 4670 5036 5945 6038 2278
Size: 2392 366 909 93 4674
Fragments arranged by size:
4674 2392 909 366 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3330 5110 5275 3330
Size: 1780 165 6489
Fragments arranged by size:
6489 1780 165
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2160 2204 2230 2536 2559 2671 2734
Size: 399 370 44 26 306 23 112 63
Cuts at: 2734 2831 3217 3377 3447 3497 3752 3927 3935
Size: 97 386 160 70 50 255 175 8
Cuts at: 3935 3984 4227 4272 4377 4830 5522 5639 5663
Size: 49 243 45 105 453 692 117 24
Cuts at: 5663 5753 6495 6580 7651 1391
Size: 90 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 692 453 399 386 370
306 255 243 175 160 117 112 105
97 90 85 70 63 50 49 45
44 26 24 23 8
BspEI T'CCGG_A
Cuts at: 3805 4740 8097 3805
Size: 935 3357 4142
Fragments arranged by size:
4142 3357 935
BspHI T'CATG_A
Cuts at: 4875 6323 8053 4875
Size: 1448 1730 5256
Fragments arranged by size:
5256 1730 1448
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2242 2312 2358 3667 4720 5789 844
Size: 1398 70 46 1309 1053 1069 3489
Fragments arranged by size:
3489 1398 1309 1069 1053 70 46
BsrBI CCG'CTC
Cuts at: 1394 1823 2391 4648 6321 1394
Size: 429 568 2257 1673 3507
Fragments arranged by size:
3507 2257 1673 568 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3015 3609 5322 6929 7103 65
Size: 1183 1767 594 1713 1607 174 1396
Fragments arranged by size:
1767 1713 1607 1396 1183 594 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2139 3662 5202 5363 7078 7265 1700
Size: 110 329 1523 1540 161 1715 187 2869
Fragments arranged by size:
2869 1715 1540 1523 329 187 161 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8434
BssHII G'CGCG_C
Cuts at: 1925 4968 1925
Size: 3043 5391
BssSI C'ACGA_G
Cuts at: 6494 7506 6494
Size: 1012 7422
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8434
BstBI TT'CG_AA
Cuts at: 7238 7238
Size: 8434
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8434
BstXI CCAn_nnnn'nTGG
Cuts at: 2044 3910 4474 5386 5683 2044
Size: 1866 564 912 297 4795
Fragments arranged by size:
4795 1866 912 564 297
BstYI r'GATC_y
Cuts at: 1972 2127 4176 4482 4737 4902 5764 6131 6516
Size: 155 2049 306 255 165 862 367 385
Cuts at: 6516 6533 7985 8071 8083 8429 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2049 1977 1452 862 385 367 346 306
255 165 155 86 17 12
BstZ17I GTA'TAC
Cuts at: 5395 5395
Size: 8434
Bsu36I CC'TnA_GG
Cuts at: 2877 3411 3654 2877
Size: 534 243 7657
Fragments arranged by size:
7657 534 243
BtgI C'CryG_G
Cuts at: 513 1065 3597 3775 5053 5145 5260 5592 513
Size: 552 2532 178 1278 92 115 332 3355
Fragments arranged by size:
3355 2532 1278 552 332 178 115 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4237 487
Size: 683 56 3011 4684
Fragments arranged by size:
4684 3011 683 56
BtsI GCAGTG_nn'
Cuts at: 2228 2451 2797 2933 3560 4050 4423 5471 5950
Size: 223 346 136 627 490 373 1048 479
Cuts at: 5950 6744 6764 2228
Size: 794 20 3898
Fragments arranged by size:
3898 1048 794 627 490 479 373 346
223 136 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8434
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3592 3627 529
Size: 35 3028 35 5336
Fragments arranged by size:
5336 3028 35 35
DraI TTT'AAA
Cuts at: 5738 5738
Size: 8434
DraIII CAC_nnn'GTG
Cuts at: 2188 5227 2188
Size: 3039 5395
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4070 7441 809
Size: 507 2754 3371 1802
Fragments arranged by size:
3371 2754 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 2772 2847
Size: 54 1425 50 275 18 303 639 75
Cuts at: 2847 3567 3600 3912 4197 4317 4425 4649 5204
Size: 720 33 312 285 120 108 224 555
Cuts at: 5204 5361 5881 6770 8399 8
Size: 157 520 889 1629 43
Fragments arranged by size:
1629 1425 889 720 639 555 520 312
303 285 275 224 157 120 108 75
54 50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 4197 5204 5881 1537
Size: 275 2385 1007 677 4090
Fragments arranged by size:
4090 2385 1007 677 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2253 2466 2733 3042 3296 4094 4193 4583
Size: 900 213 267 309 254 798 99 390
Cuts at: 4583 6364 1353
Size: 1781 3423
Fragments arranged by size:
3423 1781 900 798 390 309 267 254
213 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3367 3371 4591 4676 5663 7021
Size: 1064 138 1778 4 1220 85 987 1358
Cuts at: 7021 7395 7541 387
Size: 374 146 1280
Fragments arranged by size:
1778 1358 1280 1220 1064 987 374 146
138 85 4
EcoICRI GAG'CTC
Cuts at: 3375 3375
Size: 8434
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3201 3354 3932 4166 4324 4373 4382
Size: 393 1569 153 578 234 158 49 9
Cuts at: 4382 4508 4872 5059 5735 6497 7094 7881 8149
Size: 126 364 187 676 762 597 787 268
Cuts at: 8149 1239
Size: 1524
Fragments arranged by size:
1569 1524 787 762 676 597 578 393
364 268 234 187 158 153 126 49
9
EcoNI CCTnn'n_nnAGG
Cuts at: 4628 4699 5336 4628
Size: 71 637 7726
Fragments arranged by size:
7726 637 71
EcoO109I rG'GnC_Cy
Cuts at: 2476 2555 2556 2881 3213 3238 3329 3826 4827
Size: 79 1 325 332 25 91 497 1001
Cuts at: 4827 5543 5635 5636 5659 2476
Size: 716 92 1 23 5251
Fragments arranged by size:
5251 1001 716 497 332 325 92 91
79 25 23 1 1
EcoRI G'AATT_C
Cuts at: 5743 6121 5743
Size: 378 8056
FseI GG_CCGG'CC
Cuts at: 5206 5206
Size: 8434
FspI TGC'GCA
Cuts at: 3277 4124 4997 5606 6940 3277
Size: 847 873 609 1334 4771
Fragments arranged by size:
4771 1334 873 847 609
FspAI rTGC'GCAy
Cuts at: 4997 4997
Size: 8434
HaeII r_GCGC'y
Cuts at: 1678 3434 3597 4495 4544 4816 5145 5405 7581
Size: 1756 163 898 49 272 329 260 2176
Cuts at: 7581 1678
Size: 2531
Fragments arranged by size:
2531 2176 1756 898 329 272 260 163
49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2397 2429 2825 2857 3329
Size: 32 135 32 825 32 396 32 472
Cuts at: 3329 3361 3506 3538 4289 4321 4661 4693 7075
Size: 32 145 32 751 32 340 32 2382
Cuts at: 7075 7107 7149 7181 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2382 825 751 472 396 340 145
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5778 6024 669
Size: 988 281 3840 246 3079
Fragments arranged by size:
3840 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8434
HpaI GTT'AAC
Cuts at: 6024 6024
Size: 8434
KasI G'GCGC_C
Cuts at: 3593 5141 3593
Size: 1548 6886
KpnI G_GTAC'C
Cuts at: 5759 5759
Size: 8434
MfeI C'AATT_G
Cuts at: 6033 6033
Size: 8434
MluI A'CGCG_T
Cuts at: 8310 8310
Size: 8434
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3510 4779 7548 7732 1305
Size: 2205 1269 2769 184 2007
Fragments arranged by size:
2769 2205 2007 1269 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2135 2774 3569 3602 3914
Size: 54 1425 343 303 639 795 33 312
Cuts at: 3914 4427 10
Size: 513 4017
Fragments arranged by size:
4017 1425 795 639 513 343 312 303
54 33
MslI CAynn'nnrTG
Cuts at: 518 1166 4778 5022 5529 6392 6751 6910 518
Size: 648 3612 244 507 863 359 159 2042
Fragments arranged by size:
3612 2042 863 648 507 359 244 159
MspA1I CmG'CkG
Cuts at: 1606 2118 2169 3309 4016 4657 4888 5055 5509
Size: 512 51 1140 707 641 231 167 454
Cuts at: 5509 5620 5850 6527 7675 7920 1606
Size: 111 230 677 1148 245 2120
Fragments arranged by size:
2120 1148 1140 707 677 641 512 454
245 231 230 167 111 51
NaeI GCC'GGC
Cuts at: 3664 5204 3664
Size: 1540 6894
NarI GG'CG_CC
Cuts at: 3594 5142 3594
Size: 1548 6886
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7882
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8434
NgoMIV G'CCGG_C
Cuts at: 3662 5202 3662
Size: 1540 6894
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8434
NotI GC'GGCC_GC
Cuts at: 5881 5881
Size: 8434
NspI r_CATG'y
Cuts at: 1132 1229 2611 2758 3010 3276 5534 5792 7337
Size: 97 1382 147 252 266 2258 258 1545
Cuts at: 7337 1132
Size: 2229
Fragments arranged by size:
2258 2229 1545 1382 266 258 252 147
97
PasI CC'CwG_GG
Cuts at: 1348 3225 4291 4701 1348
Size: 1877 1066 410 5081
Fragments arranged by size:
5081 1877 1066 410
PciI A'CATG_T
Cuts at: 7333 7333
Size: 8434
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2770 3179 4593 1108
Size: 1662 409 1414 4949
Fragments arranged by size:
4949 1662 1414 409
PfoI T'CCnGG_A
Cuts at: 2267 2415 3318 4485 2267
Size: 148 903 1167 6216
Fragments arranged by size:
6216 1167 903 148
PmeI GTTT'AAAC
Cuts at: 5738 5738
Size: 8434
PmlI CAC'GTG
Cuts at: 2197 3427 2197
Size: 1230 7204
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3035 3067 6503 6535 8016 8048 3035
Size: 32 3436 32 1481 32 3421
Fragments arranged by size:
3436 3421 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2476 3826 5543 2476
Size: 1350 1717 5367
Fragments arranged by size:
5367 1717 1350
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8434
PsiI TTA'TAA
Cuts at: 6004 6004
Size: 8434
PspOMI G'GGCC_C
Cuts at: 2156 2555 2667 3213 4826 5635 5659 2156
Size: 399 112 546 1613 809 24 4931
Fragments arranged by size:
4931 1613 809 546 399 112 24
PspXI vC'TCGA_Gb
Cuts at: 4220 5629 4220
Size: 1409 7025
PstI C_TGCA'G
Cuts at: 830 2253 4332 5328 5786 830
Size: 1423 2079 996 458 3478
Fragments arranged by size:
3478 2079 1423 996 458
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8434
PvuII CAG'CTG
Cuts at: 4657 5509 5620 4657
Size: 852 111 7471
Fragments arranged by size:
7471 852 111
SacI G_AGCT'C
Cuts at: 3377 3377
Size: 8434
SacII CC_GC'GG
Cuts at: 5056 5056
Size: 8434
SalI G'TCGA_C
Cuts at: 1936 5776 1936
Size: 3840 4594
SanDI GG'GwC_CC
Cuts at: 3826 5543 3826
Size: 1717 6717
SbfI CC_TGCA'GG
Cuts at: 2253 5786 2253
Size: 3533 4901
ScaI AGT'ACT
Cuts at: 6682 8394 6682
Size: 1712 6722
SexAI A'CCwGG_T
Cuts at: 2055 2055
Size: 8434
SfcI C'TryA_G
Cuts at: 826 1045 2249 4328 4684 5324 5456 5782 6917
Size: 219 1204 2079 356 640 132 326 1135
Cuts at: 6917 7598 7789 826
Size: 681 191 1471
Fragments arranged by size:
2079 1471 1204 1135 681 640 356 326
219 191 132
SfoI GGC'GCC
Cuts at: 3595 5143 3595
Size: 1548 6886
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8434
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8434
SmaI CCC'GGG
Cuts at: 1872 2161 2818 5664 5761 1872
Size: 289 657 2846 97 4545
Fragments arranged by size:
4545 2846 657 289 97
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4220 5629 6538 7439 7701 820
Size: 197 926 2277 1409 909 901 262 1553
Fragments arranged by size:
2277 1553 1409 926 909 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6225 493
Size: 5732 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8434
SphI G_CATG'C
Cuts at: 1132 2611 2758 3010 3276 5534 5792 1132
Size: 1479 147 252 266 2258 258 3774
Fragments arranged by size:
3774 2258 1479 266 258 252 147
SspI AAT'ATT
Cuts at: 5 52 6358 5
Size: 47 6306 2081
Fragments arranged by size:
6306 2081 47
StuI AGG'CCT
Cuts at: 1517 2681 3416 3629 3852 1517
Size: 1164 735 213 223 6099
Fragments arranged by size:
6099 1164 735 223 213
StyI C'CwwG_G
Cuts at: 513 1065 2238 3099 3417 5127 5559 5873 513
Size: 552 1173 861 318 1710 432 314 3074
Fragments arranged by size:
3074 1710 1173 861 552 432 318 314
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2485 3238 3469 4399 5306 5689 6461
Size: 1255 905 753 231 930 907 383 772
Cuts at: 6461 6478 6631 6816 7261 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 930 907 905 772 753 445
383 231 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2460 4527 6680
Size: 274 80 33 51 1443 483 2067 2153
Cuts at: 6680 8392 96
Size: 1712 138
Fragments arranged by size:
2153 2067 1712 1443 483 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2186 2218 2961 2993 4402 4434 4481 4513 4537
Size: 32 743 32 1409 32 47 32 24
Cuts at: 4537 4569 4845 4877 8394 8426 2186
Size: 32 276 32 3517 32 2194
Fragments arranged by size:
3517 2194 1409 743 276 47 32 32
32 32 32 32 32 24
Tth111I GACn'n_nGTC
Cuts at: 1316 4838 5153 1316
Size: 3522 315 4597
Fragments arranged by size:
4597 3522 315
XbaI T'CTAG_A
Cuts at: 5770 5770
Size: 8434
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4348 4471 4348
Size: 123 8311
XhoI C'TCGA_G
Cuts at: 1943 4220 5629 1943
Size: 2277 1409 4748
Fragments arranged by size:
4748 2277 1409
XmaI C'CCGG_G
Cuts at: 1870 2159 2816 5662 5759 1870
Size: 289 657 2846 97 4545
Fragments arranged by size:
4545 2846 657 289 97
XmnI GAAnn'nnTTC
Cuts at: 6563 6563
Size: 8434
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4837 6242 276
Size: 53 83 186 936 3303 1405 2468
Fragments arranged by size:
3303 2468 1405 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI EcoRV FalI NruI
NsiI PacI PsrI RsrII SapI SfiI SrfI SwaI