Restriction map for
pFN21AE6704
(Circular) (Six-base) MAPSORT of: pFN21AE6704.seq Check: 5011 from: 1 to: 7888
pFN21AE6704 7888 bp
With 182 enzymes: SgfI *
June 19, 2019 18:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3287 3477 5698 278
Size: 53 83 186 936 1751 190 2221 2468
Fragments arranged by size:
2468 2221 1751 936 190 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5231 1937
Size: 3294 4594
Acc65I G'GTAC_C
Cuts at: 5209 5209
Size: 7888
AclI AA'CG_TT
Cuts at: 1563 6015 6388 1563
Size: 4452 373 3063
Fragments arranged by size:
4452 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2099 2794 3829 3986 4220 4271 4613 5951
Size: 467 695 1035 157 234 51 342 1338
Cuts at: 5951 7335 7603 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1338 1035 695 467 342 268
234 157 51
AfeI AGC'GCT
Cuts at: 3971 5151 3971
Size: 1180 6708
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7691
AflIII A'CryG_T
Cuts at: 1203 3648 6787 7764 1203
Size: 2445 3139 977 1327
Fragments arranged by size:
3139 2445 1327 977
AgeI A'CCGG_T
Cuts at: 2469 6719 2469
Size: 4250 3638
AhdI GACnn_n'nnGTC
Cuts at: 6617 6617
Size: 7888
AleI CACnn'nnGTG
Cuts at: 1166 3302 1166
Size: 2136 5752
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2693 4385 1184
Size: 1509 1692 4687
Fragments arranged by size:
4687 1692 1509
AlwNI CAG_nnn'CTG
Cuts at: 2533 2817 3256 3892 3991 4243 4555 4660 4756
Size: 284 439 636 99 252 312 105 96
Cuts at: 4756 4927 5631 7203 2533
Size: 171 704 1572 3218
Fragments arranged by size:
3218 1572 704 636 439 312 284 252
171 105 99 96
ApaI G_GGCC'C
Cuts at: 3352 3352
Size: 7888
ApaLI G'TGCA_C
Cuts at: 4132 5945 7101 4132
Size: 1813 1156 4919
Fragments arranged by size:
4919 1813 1156
ApoI r'AATT_y
Cuts at: 1493 2411 3560 4315 5197 5423 5575 7684 1493
Size: 918 1149 755 882 226 152 2109 1697
Fragments arranged by size:
2109 1697 1149 918 882 755 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6442 160
Size: 872 5410 1606
Fragments arranged by size:
5410 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7888
AvaI C'yCGr_G
Cuts at: 1870 1943 2063 2624 2861 4700 5213 1870
Size: 73 120 561 237 1839 513 4545
Fragments arranged by size:
4545 1839 561 513 237 120 73
AvrII C'CTAG_G
Cuts at: 2443 5032 2443
Size: 2589 5299
BamHI G'GATC_C
Cuts at: 3786 5218 5585 3786
Size: 1432 367 6089
Fragments arranged by size:
6089 1432 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2934 4004 4257 4475 4560
Size: 325 217 627 1147 1070 253 218 85
Cuts at: 4560 5209 6664 618
Size: 649 1455 1842
Fragments arranged by size:
1842 1455 1147 1070 649 627 325 253
218 217 85
BanII G_rGCy'C
Cuts at: 1391 2829 2850 2901 3352 3411 3833 4784 5207
Size: 1438 21 51 451 59 422 951 423
Cuts at: 5207 1391
Size: 4072
Fragments arranged by size:
4072 1438 951 451 423 422 59 51
21
BbeI G_GCGC'C
Cuts at: 4008 4008
Size: 7888
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2433 3157 3477 4035 928
Size: 971 534 724 320 558 4781
Fragments arranged by size:
4781 971 724 558 534 320
BbvCI CC'TCA_GC
Cuts at: 2462 4890 2462
Size: 2428 5460
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5040 5074 6079 6113 1903
Size: 34 50 34 3019 34 1005 34 3678
Fragments arranged by size:
3678 3019 1005 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2287 5780 6990 2287
Size: 3493 1210 3185
Fragments arranged by size:
3493 3185 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7888
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4779 5079 6499
Size: 107 122 71 631 605 3107 300 1420
Cuts at: 6499 136
Size: 1525
Fragments arranged by size:
3107 1525 1420 631 605 300 122 107
71
BglII A'GATC_T
Cuts at: 4596 7883 4596
Size: 3287 4601
BlpI GC'TnA_GC
Cuts at: 4273 5069 5305 4273
Size: 796 236 6856
Fragments arranged by size:
6856 796 236
Bme1580I G_kGCm'C
Cuts at: 1790 3352 4136 5949 7105 1790
Size: 1562 784 1813 1156 2573
Fragments arranged by size:
2573 1813 1562 1156 784
BmgBI CAC'GTC
Cuts at: 1318 3625 1318
Size: 2307 5581
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2713 4390 4559
Size: 416 53 83 399 24 1296 1677 169
Cuts at: 4559 4879 5619 6577 442
Size: 320 740 958 1753
Fragments arranged by size:
1753 1677 1296 958 740 416 399 320
169 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2580 1056
Size: 1524 6364
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2238 4306 4651 2238
Size: 2068 345 5475
Fragments arranged by size:
5475 2068 345
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2233 2825 3086 3230 3278 3344 3749 4252
Size: 994 592 261 144 48 66 405 503
Cuts at: 4252 4457 4541 4733 4799 4904 6548 1239
Size: 205 84 192 66 105 1644 2579
Fragments arranged by size:
2579 1644 994 592 503 405 261 205
192 144 105 84 66 66 48
Bpu10I CC'TnA_GC
Cuts at: 2462 2823 3006 3893 4676 4890 2462
Size: 361 183 887 783 214 5460
Fragments arranged by size:
5460 887 783 361 214 183
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2885 3568 4867 6013 6878 7176 2885
Size: 683 1299 1146 865 298 3597
Fragments arranged by size:
3597 1299 1146 865 683 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2588 3707 3757 3955 6551 882
Size: 617 1089 1119 50 198 2596 2219
Fragments arranged by size:
2596 2219 1119 1089 617 198 50
BsaAI yAC'GTr
Cuts at: 493 1204 3175 4740 5679 493
Size: 711 1971 1565 939 2702
Fragments arranged by size:
2702 1971 1565 939 711
BsaBI GATnn'nnATC
Cuts at: 7882 7882
Size: 7888
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3284 3474 4005 4101
Size: 53 83 186 936 1751 190 531 96
Cuts at: 4101 5695 6077 6712 275
Size: 1594 382 635 1451
Fragments arranged by size:
1751 1594 1451 936 635 531 382 190
186 96 83 53
BsaWI w'CCGG_w
Cuts at: 2469 3447 6321 6719 6993 7140 7551 2469
Size: 978 2874 398 274 147 411 2806
Fragments arranged by size:
2874 2806 978 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3860 3890 3914 3935 3944
Size: 30 165 30 2457 30 24 21 9
Cuts at: 3944 3965 1178
Size: 21 5101
Fragments arranged by size:
5101 2457 165 30 30 30 24 21
21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2219 2630 2963 3449 3725 3884 3887 3943
Size: 1033 411 333 486 276 159 3 56
Cuts at: 3943 1186
Size: 5131
Fragments arranged by size:
5131 1033 486 411 333 276 159 56
3
BseYI C'CCAG_C
Cuts at: 2116 2175 2828 2943 3225 4690 4777 7091 7587
Size: 59 653 115 282 1465 87 2314 496
Cuts at: 7587 2116
Size: 2417
Fragments arranged by size:
2417 2314 1465 653 496 282 115 87
59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3977 4091 4420 1163
Size: 573 2241 114 329 4631
Fragments arranged by size:
4631 2241 573 329 114
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5338 6099 7127 1540
Size: 275 186 3337 761 1028 2301
Fragments arranged by size:
3337 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2502 2850 2901 3072 3392 3945 4046 4136 4473
Size: 348 51 171 320 553 101 90 337
Cuts at: 4473 4815 5949 6034 7105 2502
Size: 342 1134 85 1071 3285
Fragments arranged by size:
3285 1134 1071 553 348 342 337 320
171 101 90 85 51
BsmI GAATG_Cn'
Cuts at: 2792 5399 5492 2792
Size: 2607 93 5188
Fragments arranged by size:
5188 2607 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3481 3481
Size: 7888
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2502 2829 2850 2901 3072 3352 3392
Size: 399 712 327 21 51 171 280 40
Cuts at: 3392 3411 3833 3945 4046 4136 4473 4784 4815
Size: 19 422 112 101 90 337 311 31
Cuts at: 4815 5207 5949 6034 7105 1391
Size: 392 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 712 422 399 392 337
327 311 280 171 112 101 90 85
51 40 31 21 19
BspEI T'CCGG_A
Cuts at: 3447 7551 3447
Size: 4104 3784
BspHI T'CATG_A
Cuts at: 5777 7507 5777
Size: 1730 6158
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2506 3248 3327 5243 844
Size: 1662 742 79 1916 3489
Fragments arranged by size:
3489 1916 1662 742 79
BsrBI CCG'CTC
Cuts at: 1394 1823 2239 5775 1394
Size: 429 416 3536 3507
Fragments arranged by size:
3536 3507 429 416
BsrDI GCAATG_nn'
Cuts at: 65 1248 2440 3924 6383 6557 65
Size: 1183 1192 1484 2459 174 1396
Fragments arranged by size:
2459 1484 1396 1192 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2156 2469 4773 6532 6719 1700
Size: 110 346 313 2304 1759 187 2869
Fragments arranged by size:
2869 2304 1759 346 313 187 110
BsrGI T'GTAC_A
Cuts at: 96 3306 96
Size: 3210 4678
BssHII G'CGCG_C
Cuts at: 1925 2438 2832 1925
Size: 513 394 6981
Fragments arranged by size:
6981 513 394
BssSI C'ACGA_G
Cuts at: 5948 6960 5948
Size: 1012 6876
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7888
BstBI TT'CG_AA
Cuts at: 6692 6692
Size: 7888
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7888
BstXI CCAn_nnnn'nTGG
Cuts at: 2053 3043 3082 2053
Size: 990 39 6859
Fragments arranged by size:
6859 990 39
BstYI r'GATC_y
Cuts at: 1972 2130 2374 3087 3786 3861 4330 4596 5218
Size: 158 244 713 699 75 469 266 622
Cuts at: 5218 5585 5970 5987 7439 7525 7537 7883 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 713 699 622 469 385 367
346 266 244 158 86 75 17 12
Bsu36I CC'TnA_GG
Cuts at: 2922 4244 4535 4727 2922
Size: 1322 291 192 6083
Fragments arranged by size:
6083 1322 291 192
BtgI C'CryG_G
Cuts at: 513 1065 2562 2640 2781 2961 3036 513
Size: 552 1497 78 141 180 75 5365
Fragments arranged by size:
5365 1497 552 180 141 78 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7149
Fragments arranged by size:
7149 683 56
BtsI GCAGTG_nn'
Cuts at: 3297 3309 3788 4608 5404 6198 6218 3297
Size: 12 479 820 796 794 20 4967
Fragments arranged by size:
4967 820 796 794 479 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7888
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7853
DraI TTT'AAA
Cuts at: 5192 5192
Size: 7888
DraIII CAC_nnn'GTG
Cuts at: 2306 2692 3204 2306
Size: 386 512 6990
Fragments arranged by size:
6990 512 386
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5045 6895 809
Size: 507 3729 1850 1802
Fragments arranged by size:
3729 1850 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5335 6224 7853
Size: 54 1425 50 275 18 3505 889 1629
Cuts at: 7853 8
Size: 43
Fragments arranged by size:
3505 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5335 1537
Size: 275 3523 4090
Fragments arranged by size:
4090 3523 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2726 2750 3200 3728 4247 5818 1353
Size: 1373 24 450 528 519 1571 3423
Fragments arranged by size:
3423 1571 1373 528 519 450 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2723 6475 6849 6995 387
Size: 1064 138 1134 3752 374 146 1280
Fragments arranged by size:
3752 1280 1134 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2848 2899 2848
Size: 51 7837
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2099 2233 2794 2825 3086 3230 3278
Size: 393 467 134 561 31 261 144 48
Cuts at: 3278 3344 3749 3829 3986 4220 4252 4271 4457
Size: 66 405 80 157 234 32 19 186
Cuts at: 4457 4541 4613 4733 4799 4904 5951 6548 7335
Size: 84 72 120 66 105 1047 597 787
Cuts at: 7335 7603 1239
Size: 268 1524
Fragments arranged by size:
1524 1047 787 597 561 467 405 393
268 261 234 186 157 144 134 120
105 84 80 72 66 66 48 32
31 19
EcoNI CCTnn'n_nnAGG
Cuts at: 3431 3590 3707 3824 3431
Size: 159 117 117 7495
Fragments arranged by size:
7495 159 117 117
EcoO109I rG'GnC_Cy
Cuts at: 2254 2281 2927 3348 3349 3442 3889 4285 4381
Size: 27 646 421 1 93 447 396 96
Cuts at: 4381 4696 4723 4873 2254
Size: 315 27 150 5269
Fragments arranged by size:
5269 646 447 421 396 315 150 96
93 27 27 1
EcoRI G'AATT_C
Cuts at: 3560 5197 5575 3560
Size: 1637 378 5873
Fragments arranged by size:
5873 1637 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2372 3139 2372
Size: 767 7121
FspI TGC'GCA
Cuts at: 6394 6394
Size: 7888
HaeII r_GCGC'y
Cuts at: 1678 2623 2869 3973 3984 4008 5153 7035 1678
Size: 945 246 1104 11 24 1145 1882 2531
Fragments arranged by size:
2531 1882 1145 1104 945 246 24 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4414 4446 6529 6561 6603
Size: 32 135 32 2842 32 2083 32 42
Cuts at: 6603 6635 1373
Size: 32 2626
Fragments arranged by size:
2842 2626 2083 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2334 2458 3639 5232 5478 669
Size: 988 281 396 124 1181 1593 246 3079
Fragments arranged by size:
3079 1593 1181 988 396 281 246 124
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7888
HpaI GTT'AAC
Cuts at: 5478 5478
Size: 7888
KasI G'GCGC_C
Cuts at: 4004 4004
Size: 7888
KpnI G_GTAC'C
Cuts at: 5213 5213
Size: 7888
MfeI C'AATT_G
Cuts at: 5487 5487
Size: 7888
MluI A'CGCG_T
Cuts at: 3648 7764 3648
Size: 4116 3772
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4330 4479 5074 7002 7186 1305
Size: 3025 149 595 1928 184 2007
Fragments arranged by size:
3025 2007 1928 595 184 149
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6066
Fragments arranged by size:
6066 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3302 3767 3999 5846 6205 6364 518
Size: 648 2136 465 232 1847 359 159 2042
Fragments arranged by size:
2136 2042 1847 648 465 359 232 159
MspA1I CmG'CkG
Cuts at: 1606 2179 2814 3256 3849 4124 4435 5304 5981
Size: 573 635 442 593 275 311 869 677
Cuts at: 5981 7129 7374 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 869 677 635 593 573 442
311 275 245
NaeI GCC'GGC
Cuts at: 4775 4775
Size: 7888
NarI GG'CG_CC
Cuts at: 4005 4005
Size: 7888
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7336
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7888
NgoMIV G'CCGG_C
Cuts at: 4773 4773
Size: 7888
NheI G'CTAG_C
Cuts at: 1052 2576 1052
Size: 1524 6364
NotI GC'GGCC_GC
Cuts at: 5335 5335
Size: 7888
NspI r_CATG'y
Cuts at: 1132 1229 2340 2797 3083 3313 5246 6791 1132
Size: 97 1111 457 286 230 1933 1545 2229
Fragments arranged by size:
2229 1933 1545 1111 457 286 230 97
PasI CC'CwG_GG
Cuts at: 1348 2136 4177 4984 1348
Size: 788 2041 807 4252
Fragments arranged by size:
4252 2041 807 788
PciI A'CATG_T
Cuts at: 6787 6787
Size: 7888
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2306 1108
Size: 1198 6690
PfoI T'CCnGG_A
Cuts at: 2841 3287 3555 3585 3864 4833 2841
Size: 446 268 30 279 969 5896
Fragments arranged by size:
5896 969 446 279 268 30
PmeI GTTT'AAAC
Cuts at: 5192 5192
Size: 7888
PmlI CAC'GTG
Cuts at: 4740 4740
Size: 7888
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5957 5989 7470 7502 5957
Size: 32 1481 32 6343
Fragments arranged by size:
6343 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2254 2281 2927 3442 4285 4696 2254
Size: 27 646 515 843 411 5446
Fragments arranged by size:
5446 843 646 515 411 27
PshAI GACnn'nnGTC
Cuts at: 1613 3283 1613
Size: 1670 6218
PsiI TTA'TAA
Cuts at: 5458 5458
Size: 7888
PspOMI G'GGCC_C
Cuts at: 3348 3348
Size: 7888
PspXI vC'TCGA_Gb
Cuts at: 2063 2063
Size: 7888
PstI C_TGCA'G
Cuts at: 830 2762 3304 3825 3977 4297 4410 4574 4916
Size: 1932 542 521 152 320 113 164 342
Cuts at: 4916 5240 830
Size: 324 3478
Fragments arranged by size:
3478 1932 542 521 342 324 320 164
152 113
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7888
PvuII CAG'CTG
Cuts at: 2179 2814 3256 3849 4435 2179
Size: 635 442 593 586 5632
Fragments arranged by size:
5632 635 593 586 442
SacI G_AGCT'C
Cuts at: 2850 2901 2850
Size: 51 7837
SalI G'TCGA_C
Cuts at: 1936 5230 1936
Size: 3294 4594
SanDI GG'GwC_CC
Cuts at: 2927 2927
Size: 7888
SapI GCTCTTCn'nnn_
Cuts at: 2726 2726
Size: 7888
SbfI CC_TGCA'GG
Cuts at: 5240 5240
Size: 7888
ScaI AGT'ACT
Cuts at: 6136 7848 6136
Size: 1712 6176
SexAI A'CCwGG_T
Cuts at: 2283 2302 2283
Size: 19 7869
SfcI C'TryA_G
Cuts at: 826 1045 2389 2758 3300 3532 3821 3973 4012
Size: 219 1344 369 542 232 289 152 39
Cuts at: 4012 4105 4293 4406 4570 4912 5236 6371 7052
Size: 93 188 113 164 342 324 1135 681
Cuts at: 7052 7243 826
Size: 191 1471
Fragments arranged by size:
1471 1344 1135 681 542 369 342 324
289 232 219 191 188 164 152 113
93 39
SfoI GGC'GCC
Cuts at: 4006 4006
Size: 7888
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7888
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7888
SmaI CCC'GGG
Cuts at: 1872 5215 1872
Size: 3343 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2063 2900 3547 4846 5992 6893
Size: 197 926 120 837 647 1299 1146 901
Cuts at: 6893 7155 820
Size: 262 1553
Fragments arranged by size:
1553 1299 1146 926 901 837 647 262
197 120
SnaBI TAC'GTA
Cuts at: 493 5679 493
Size: 5186 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7888
SphI G_CATG'C
Cuts at: 1132 2797 5246 1132
Size: 1665 2449 3774
Fragments arranged by size:
3774 2449 1665
SspI AAT'ATT
Cuts at: 5 52 5812 5
Size: 47 5760 2081
Fragments arranged by size:
5760 2081 47
StuI AGG'CCT
Cuts at: 1517 2210 1517
Size: 693 7195
StyI C'CwwG_G
Cuts at: 513 1065 2443 2725 3393 3834 4956 5032 5327
Size: 552 1378 282 668 441 1122 76 295
Cuts at: 5327 513
Size: 3074
Fragments arranged by size:
3074 1378 1122 668 552 441 295 282
76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2204 3217 3385 3656 4578 5021 5915
Size: 1255 624 1013 168 271 922 443 894
Cuts at: 5915 5932 6085 6270 6715 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1013 922 894 624 445 443
271 185 168 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3306 3576 6134
Size: 274 80 33 51 1443 1329 270 2558
Cuts at: 6134 7846 96
Size: 1712 138
Fragments arranged by size:
2558 1712 1443 1329 274 270 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3935 3967 7848 7880 3935
Size: 32 3881 32 3943
Fragments arranged by size:
3943 3881 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2399 1316
Size: 1083 6805
XbaI T'CTAG_A
Cuts at: 5224 5224
Size: 7888
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2208 3724 4486 2208
Size: 1516 762 5610
Fragments arranged by size:
5610 1516 762
XhoI C'TCGA_G
Cuts at: 1943 2063 1943
Size: 120 7768
XmaI C'CCGG_G
Cuts at: 1870 5213 1870
Size: 3343 4545
XmnI GAAnn'nnTTC
Cuts at: 2084 2720 3500 6017 2084
Size: 636 780 2517 3955
Fragments arranged by size:
3955 2517 780 636
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3285 3475 5696 276
Size: 53 83 186 936 1751 190 2221 2468
Fragments arranged by size:
2468 2221 1751 936 190 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BfrBI BsiWI BstZ17I EcoRV
FseI FspAI NruI NsiI PacI PsrI RsrII SacII
SfiI SrfI SwaI