Restriction map for
pFN21AE6703
(Circular) (Six-base) MAPSORT of: pFN21AE6703.seq Check: 1291 from: 1 to: 7351
pFN21AE6703 7351 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5161 278
Size: 53 83 186 936 3625 2468
Fragments arranged by size:
3625 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4694 1937
Size: 2757 4594
Acc65I G'GTAC_C
Cuts at: 4672 4672
Size: 7351
AclI AA'CG_TT
Cuts at: 1563 4336 5478 5851 1563
Size: 2773 1142 373 3063
Fragments arranged by size:
3063 2773 1142 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3033 3792 4255 5414 6798 7066 1632
Size: 1401 759 463 1159 1384 268 1917
Fragments arranged by size:
1917 1401 1384 1159 759 463 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7154
AflIII A'CryG_T
Cuts at: 1203 6250 7227 1203
Size: 5047 977 1327
Fragments arranged by size:
5047 1327 977
AgeI A'CCGG_T
Cuts at: 6182 6182
Size: 7351
AhdI GACnn_n'nnGTC
Cuts at: 6080 6080
Size: 7351
AleI CACnn'nnGTG
Cuts at: 1166 2708 1166
Size: 1542 5809
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7351
AlwNI CAG_nnn'CTG
Cuts at: 3245 5094 6666 3245
Size: 1849 1572 3930
Fragments arranged by size:
3930 1849 1572
ApaI G_GGCC'C
Cuts at: 4294 4294
Size: 7351
ApaLI G'TGCA_C
Cuts at: 2711 5408 6564 2711
Size: 2697 1156 3498
Fragments arranged by size:
3498 2697 1156
ApoI r'AATT_y
Cuts at: 1493 2307 2511 2783 2847 2856 3800 3858 4094
Size: 814 204 272 64 9 944 58 236
Cuts at: 4094 4660 4886 5038 7147 1493
Size: 566 226 152 2109 1697
Fragments arranged by size:
2109 1697 944 814 566 272 236 226
204 152 64 58 9
AseI AT'TA_AT
Cuts at: 160 1032 4250 5905 160
Size: 872 3218 1655 1606
Fragments arranged by size:
3218 1655 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7351
AvaI C'yCGr_G
Cuts at: 1870 1943 2403 4676 1870
Size: 73 460 2273 4545
Fragments arranged by size:
4545 2273 460 73
BamHI G'GATC_C
Cuts at: 2605 4073 4681 5048 2605
Size: 1468 608 367 4908
Fragments arranged by size:
4908 1468 608 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2191 3581 4103 4672 6127
Size: 325 217 627 404 1390 522 569 1455
Cuts at: 6127 618
Size: 1842
Fragments arranged by size:
1842 1455 1390 627 569 522 404 325
217
BanII G_rGCy'C
Cuts at: 1391 4294 4670 1391
Size: 2903 376 4072
Fragments arranged by size:
4072 2903 376
BbeI G_GCGC'C
Cuts at: 4107 4107
Size: 7351
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6380
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5542 5576 1903
Size: 34 3605 34 3678
Fragments arranged by size:
3678 3605 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4223 4319 5243 6453 4223
Size: 96 924 1210 5121
Fragments arranged by size:
5121 1210 924 96
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7351
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5962 136
Size: 107 122 71 631 605 4290 1525
Fragments arranged by size:
4290 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4530 7346 4530
Size: 2816 4535
BlpI GC'TnA_GC
Cuts at: 4768 4768
Size: 7351
Bme1580I G_kGCm'C
Cuts at: 1790 2715 4294 5412 6568 1790
Size: 925 1579 1118 1156 2573
Fragments arranged by size:
2573 1579 1156 1118 925
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7351
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2829 3191 4289
Size: 416 53 83 399 24 1412 362 1098
Cuts at: 4289 5082 6040 442
Size: 793 958 1753
Fragments arranged by size:
1753 1412 1098 958 793 416 399 362
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2726 1056
Size: 1670 5681
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4250 4250
Size: 7351
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2149 2775 3657 3837 6011 1239
Size: 910 626 882 180 2174 2579
Fragments arranged by size:
2579 2174 910 882 626 180
Bpu10I CC'TnA_GC
Cuts at: 2187 2187
Size: 7351
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5476 6341 6639 5476
Size: 865 298 6188
Fragments arranged by size:
6188 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6014 882
Size: 617 4515 2219
Fragments arranged by size:
4515 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2521 3616 3628 4628 5142 493
Size: 711 1317 1095 12 1000 514 2702
Fragments arranged by size:
2702 1317 1095 1000 711 514 12
BsaBI GATnn'nnATC
Cuts at: 3299 7345 3299
Size: 4046 3305
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3257 4104 5158 5540
Size: 53 83 186 936 1724 847 1054 382
Cuts at: 5540 6175 275
Size: 635 1451
Fragments arranged by size:
1724 1451 1054 936 847 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5784 6182 6456 6603 7014 5784
Size: 398 274 147 411 6121
Fragments arranged by size:
6121 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7126
Fragments arranged by size:
7126 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2081 3500 3840 1186
Size: 895 1419 340 4697
Fragments arranged by size:
4697 1419 895 340
BseYI C'CCAG_C
Cuts at: 2032 4286 6554 7050 2032
Size: 2254 2268 496 2333
Fragments arranged by size:
2333 2268 2254 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3998 1163
Size: 573 2262 4516
Fragments arranged by size:
4516 2262 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4801 5562 6590 1540
Size: 275 186 2800 761 1028 2301
Fragments arranged by size:
2800 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2715 5412 5497 6568 2715
Size: 2697 85 1071 3498
Fragments arranged by size:
3498 2697 1071 85
BsmI GAATG_Cn'
Cuts at: 3374 4862 4955 3374
Size: 1488 93 5770
Fragments arranged by size:
5770 1488 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2715 4294 4670 5412 5497 6568 1391
Size: 399 925 1579 376 742 85 1071 2174
Fragments arranged by size:
2174 1579 1071 925 742 399 376 85
BspEI T'CCGG_A
Cuts at: 7014 7014
Size: 7351
BspHI T'CATG_A
Cuts at: 3916 5240 6970 3916
Size: 1324 1730 4297
Fragments arranged by size:
4297 1730 1324
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3818 4706 844
Size: 2974 888 3489
Fragments arranged by size:
3489 2974 888
BsrBI CCG'CTC
Cuts at: 1394 1823 2119 5238 1394
Size: 429 296 3119 3507
Fragments arranged by size:
3507 3119 429 296
BsrDI GCAATG_nn'
Cuts at: 65 1248 2022 5846 6020 65
Size: 1183 774 3824 174 1396
Fragments arranged by size:
3824 1396 1183 774 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2501 3525 5995 6182 1700
Size: 110 691 1024 2470 187 2869
Fragments arranged by size:
2869 2470 1024 691 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7351
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7351
BssSI C'ACGA_G
Cuts at: 5411 6423 5411
Size: 1012 6339
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4597 794
Size: 3803 3548
BstBI TT'CG_AA
Cuts at: 6155 6155
Size: 7351
BstEII G'GTnAC_C
Cuts at: 1184 4346 1184
Size: 3162 4189
BstXI CCAn_nnnn'nTGG
Cuts at: 2480 2575 3817 2480
Size: 95 1242 6014
Fragments arranged by size:
6014 1242 95
BstYI r'GATC_y
Cuts at: 1972 2365 2605 3533 4073 4308 4382 4530 4681
Size: 393 240 928 540 235 74 148 151
Cuts at: 4681 5048 5433 5450 6902 6988 7000 7346 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 928 540 393 385 367 346
240 235 151 148 86 74 17 12
Bsu36I CC'TnA_GG
Cuts at: 3111 3972 4539 3111
Size: 861 567 5923
Fragments arranged by size:
5923 861 567
BtgI C'CryG_G
Cuts at: 513 1065 2004 3661 4210 513
Size: 552 939 1657 549 3654
Fragments arranged by size:
3654 1657 939 552 549
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6612
Fragments arranged by size:
6612 683 56
BtsI GCAGTG_nn'
Cuts at: 4867 5661 5681 4867
Size: 794 20 6537
Fragments arranged by size:
6537 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7351
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7316
DraI TTT'AAA
Cuts at: 3414 4000 4111 4655 3414
Size: 586 111 544 6110
Fragments arranged by size:
6110 586 544 111
DraIII CAC_nnn'GTG
Cuts at: 3743 3745 4305 3743
Size: 2 560 6789
Fragments arranged by size:
6789 560 2
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6358 809
Size: 507 5042 1802
Fragments arranged by size:
5042 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3523 4389 4798
Size: 54 1425 50 275 18 1693 866 409
Cuts at: 4798 5687 7316 8
Size: 889 1629 43
Fragments arranged by size:
1693 1629 1425 889 866 409 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4798 1537
Size: 275 2986 4090
Fragments arranged by size:
4090 2986 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2210 3333 3840 5281 1353
Size: 857 1123 507 1441 3423
Fragments arranged by size:
3423 1441 1123 857 507
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5938 6312 6458 387
Size: 1064 138 4349 374 146 1280
Fragments arranged by size:
4349 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2149 2775 3033 3657 3792 3837 4255
Size: 393 517 626 258 624 135 45 418
Cuts at: 4255 5414 6011 6798 7066 1239
Size: 1159 597 787 268 1524
Fragments arranged by size:
1524 1159 787 626 624 597 517 418
393 268 258 135 45
EcoNI CCTnn'n_nnAGG
Cuts at: 2955 2955
Size: 7351
EcoO109I rG'GnC_Cy
Cuts at: 3830 4290 4291 3830
Size: 460 1 6890
Fragments arranged by size:
6890 460 1
EcoRI G'AATT_C
Cuts at: 2856 4660 5038 2856
Size: 1804 378 5169
Fragments arranged by size:
5169 1804 378
EcoRV GAT'ATC
Cuts at: 2670 4267 2670
Size: 1597 5754
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4634 4634
Size: 7351
FspI TGC'GCA
Cuts at: 5857 5857
Size: 7351
HaeII r_GCGC'y
Cuts at: 1678 4107 6498 1678
Size: 2429 2391 2531
Fragments arranged by size:
2531 2429 2391
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2209 2241 2319 2351 2732
Size: 32 135 32 637 32 78 32 381
Cuts at: 2732 2764 3525 3557 4522 4554 5992 6024 6066
Size: 32 761 32 965 32 1438 32 42
Cuts at: 6066 6098 1373
Size: 32 2626
Fragments arranged by size:
2626 1438 965 761 637 381 135 78
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4695 4941 669
Size: 988 281 2757 246 3079
Fragments arranged by size:
3079 2757 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7351
HpaI GTT'AAC
Cuts at: 4941 4941
Size: 7351
KasI G'GCGC_C
Cuts at: 4103 4103
Size: 7351
KpnI G_GTAC'C
Cuts at: 4676 4676
Size: 7351
MfeI C'AATT_G
Cuts at: 4950 4950
Size: 7351
MluI A'CGCG_T
Cuts at: 7227 7227
Size: 7351
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2428 2898 6465 6649 1305
Size: 1123 470 3567 184 2007
Fragments arranged by size:
3567 2007 1123 470 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4391 10
Size: 54 1425 343 2559 2970
Fragments arranged by size:
2970 2559 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2210 2600 2708 5309 5668 5827 518
Size: 648 1044 390 108 2601 359 159 2042
Fragments arranged by size:
2601 2042 1044 648 390 359 159 108
MspA1I CmG'CkG
Cuts at: 1606 2032 2753 4767 5444 6592 6837 1606
Size: 426 721 2014 677 1148 245 2120
Fragments arranged by size:
2120 2014 1148 721 677 426 245
NaeI GCC'GGC
Cuts at: 3527 3527
Size: 7351
NarI GG'CG_CC
Cuts at: 4104 4104
Size: 7351
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5860
Fragments arranged by size:
5860 939 552
NdeI CA'TA_TG
Cuts at: 387 4519 387
Size: 4132 3219
NgoMIV G'CCGG_C
Cuts at: 3525 3525
Size: 7351
NheI G'CTAG_C
Cuts at: 1052 2722 1052
Size: 1670 5681
NotI GC'GGCC_GC
Cuts at: 4798 4798
Size: 7351
NspI r_CATG'y
Cuts at: 1132 1229 4709 6254 1132
Size: 97 3480 1545 2229
Fragments arranged by size:
3480 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7351
PciI A'CATG_T
Cuts at: 6250 6250
Size: 7351
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7351
PfoI T'CCnGG_A
Cuts at: 2126 3634 4449 2126
Size: 1508 815 5028
Fragments arranged by size:
5028 1508 815
PmeI GTTT'AAAC
Cuts at: 4655 4655
Size: 7351
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5420 5452 6933 6965 5420
Size: 32 1481 32 5806
Fragments arranged by size:
5806 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3830 3830
Size: 7351
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7351
PsiI TTA'TAA
Cuts at: 3710 4921 3710
Size: 1211 6140
PspOMI G'GGCC_C
Cuts at: 4290 4290
Size: 7351
PstI C_TGCA'G
Cuts at: 830 4703 830
Size: 3873 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7351
PvuII CAG'CTG
Cuts at: 2032 2753 2032
Size: 721 6630
SalI G'TCGA_C
Cuts at: 1936 4693 1936
Size: 2757 4594
SbfI CC_TGCA'GG
Cuts at: 4703 4703
Size: 7351
ScaI AGT'ACT
Cuts at: 3877 5599 7311 3877
Size: 1722 1712 3917
Fragments arranged by size:
3917 1722 1712
SfcI C'TryA_G
Cuts at: 826 1045 3161 3923 4699 5834 6515 6706 826
Size: 219 2116 762 776 1135 681 191 1471
Fragments arranged by size:
2116 1471 1135 776 762 681 219 191
SfoI GGC'GCC
Cuts at: 4105 4105
Size: 7351
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7351
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7351
SmaI CCC'GGG
Cuts at: 1872 4678 1872
Size: 2806 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2403 5455 6356 6618 820
Size: 197 926 460 3052 901 262 1553
Fragments arranged by size:
3052 1553 926 901 460 262 197
SnaBI TAC'GTA
Cuts at: 493 3616 3628 5142 493
Size: 3123 12 1514 2702
Fragments arranged by size:
3123 2702 1514 12
SpeI A'CTAG_T
Cuts at: 152 2582 152
Size: 2430 4921
SphI G_CATG'C
Cuts at: 1132 4709 1132
Size: 3577 3774
SspI AAT'ATT
Cuts at: 5 52 2074 5275 5
Size: 47 2022 3201 2081
Fragments arranged by size:
3201 2081 2022 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7351
StyI C'CwwG_G
Cuts at: 513 1065 2004 2473 4618 4790 513
Size: 552 939 469 2145 172 3074
Fragments arranged by size:
3074 2145 939 552 469 172
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2024 5378 5395 5548 5733 6178 325
Size: 1255 444 3354 17 153 185 445 1498
Fragments arranged by size:
3354 1498 1255 445 444 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3875 5597 7309
Size: 274 80 33 51 1443 1898 1722 1712
Cuts at: 7309 96
Size: 138
Fragments arranged by size:
1898 1722 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3731 3763 4202 4234 7311 7343 3731
Size: 32 439 32 3077 32 3739
Fragments arranged by size:
3739 3077 439 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7351
XbaI T'CTAG_A
Cuts at: 4687 4687
Size: 7351
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2599 4302 2599
Size: 1703 5648
XhoI C'TCGA_G
Cuts at: 1943 2403 1943
Size: 460 6891
XmaI C'CCGG_G
Cuts at: 1870 4676 1870
Size: 2806 4545
XmnI GAAnn'nnTTC
Cuts at: 2398 2455 2895 3048 5480 2398
Size: 57 440 153 2432 4269
Fragments arranged by size:
4269 2432 440 153 57
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5159 276
Size: 53 83 186 936 3625 2468
Fragments arranged by size:
3625 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BsiWI BsmBI BstZ17I EcoICRI FseI FspAI NruI NsiI
PacI PmlI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SrfI SwaI