Restriction map for
pFN21AE6702
(Circular) (Six-base) MAPSORT of: pFN21AE6702.seq Check: 1217 from: 1 to: 6979
pFN21AE6702 6979 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3547 3547
Size: 6979
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4789 278
Size: 53 83 186 936 3253 2468
Fragments arranged by size:
3253 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3711 4322 1937
Size: 1774 611 4594
Fragments arranged by size:
4594 1774 611
Acc65I G'GTAC_C
Cuts at: 4300 4300
Size: 6979
AclI AA'CG_TT
Cuts at: 1563 3876 5106 5479 1563
Size: 2313 1230 373 3063
Fragments arranged by size:
3063 2313 1230 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3383 3707 3782 3788 3995 4088 5042 6426
Size: 1751 324 75 6 207 93 954 1384
Cuts at: 6426 6694 1632
Size: 268 1917
Fragments arranged by size:
1917 1751 1384 954 324 268 207 93
75 6
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6782
AflIII A'CryG_T
Cuts at: 1203 5878 6855 1203
Size: 4675 977 1327
Fragments arranged by size:
4675 1327 977
AgeI A'CCGG_T
Cuts at: 5810 5810
Size: 6979
AhdI GACnn_n'nnGTC
Cuts at: 5708 5708
Size: 6979
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6979
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4212 1184
Size: 3028 3951
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2631 2663 2631
Size: 32 6947
AlwNI CAG_nnn'CTG
Cuts at: 2047 2875 3142 4722 6294 2047
Size: 828 267 1580 1572 2732
Fragments arranged by size:
2732 1580 1572 828 267
ApaLI G'TGCA_C
Cuts at: 5036 6192 5036
Size: 1156 5823
ApoI r'AATT_y
Cuts at: 1493 4288 4514 4666 6775 1493
Size: 2795 226 152 2109 1697
Fragments arranged by size:
2795 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3837 5533 160
Size: 872 2805 1696 1606
Fragments arranged by size:
2805 1696 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6979
AvaI C'yCGr_G
Cuts at: 1870 1943 2199 2541 2891 4237 4304 1870
Size: 73 256 342 350 1346 67 4545
Fragments arranged by size:
4545 1346 350 342 256 73 67
AvrII C'CTAG_G
Cuts at: 2156 2156
Size: 6979
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2237 2270 2237
Size: 33 6946
BamHI G'GATC_C
Cuts at: 4309 4676 4309
Size: 367 6612
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2798 2995 3559 3821 4028
Size: 325 217 627 1011 197 564 262 207
Cuts at: 4028 4300 5755 618
Size: 272 1455 1842
Fragments arranged by size:
1842 1455 1011 627 564 325 272 262
217 207 197
BanII G_rGCy'C
Cuts at: 1391 2150 3905 4298 1391
Size: 759 1755 393 4072
Fragments arranged by size:
4072 1755 759 393
BbeI G_GCGC'C
Cuts at: 3825 3825
Size: 6979
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2386 2674 3622 3864 3951 928
Size: 971 487 288 948 242 87 3956
Fragments arranged by size:
3956 971 948 487 288 242 87
BbvCI CC'TCA_GC
Cuts at: 3374 3993 3374
Size: 619 6360
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5170 5204 1903
Size: 34 3233 34 3678
Fragments arranged by size:
3678 3233 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2265 4871 6081 2265
Size: 2606 1210 3163
Fragments arranged by size:
3163 2606 1210
BclI T'GATC_A
Cuts at: 1548 2514 1548
Size: 966 6013
BfrBI ATG'CAT
Cuts at: 4172 4172
Size: 6979
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2345 2495 3416
Size: 107 122 71 631 605 673 150 921
Cuts at: 3416 5590 136
Size: 2174 1525
Fragments arranged by size:
2174 1525 921 673 631 605 150 122
107 71
BglII A'GATC_T
Cuts at: 3096 6974 3096
Size: 3878 3101
BlpI GC'TnA_GC
Cuts at: 2149 3383 4396 2149
Size: 1234 1013 4732
Fragments arranged by size:
4732 1234 1013
Bme1580I G_kGCm'C
Cuts at: 1790 3000 4033 4161 5040 6196 1790
Size: 1210 1033 128 879 1156 2573
Fragments arranged by size:
2573 1210 1156 1033 879 128
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6979
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2996 3098 4710
Size: 416 53 83 399 24 1579 102 1612
Cuts at: 4710 5668 442
Size: 958 1753
Fragments arranged by size:
1753 1612 1579 958 416 399 102 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6979
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2783 3279 5639 1239
Size: 1544 496 2360 2579
Fragments arranged by size:
2579 2360 1544 496
Bpu10I CC'TnA_GC
Cuts at: 3374 3904 3993 3374
Size: 530 89 6360
Fragments arranged by size:
6360 530 89
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2681 3728 4043 4169 5104 5969 6267 2681
Size: 1047 315 126 935 865 298 3393
Fragments arranged by size:
3393 1047 935 865 315 298 126
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5642 882
Size: 617 4143 2219
Fragments arranged by size:
4143 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4770 493
Size: 711 3566 2702
Fragments arranged by size:
3566 2702 711
BsaBI GATnn'nnATC
Cuts at: 6973 6973
Size: 6979
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3822 4786 5168 5803
Size: 53 83 186 936 2289 964 382 635
Cuts at: 5803 275
Size: 1451
Fragments arranged by size:
2289 1451 964 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3522 5412 5810 6084 6231 6642 3522
Size: 1890 398 274 147 411 3859
Fragments arranged by size:
3859 1890 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3380 3410 1178
Size: 30 165 30 1977 30 4747
Fragments arranged by size:
4747 1977 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2144 3275 3628 3800 3880 1186
Size: 958 1131 353 172 80 4285
Fragments arranged by size:
4285 1131 958 353 172 80
BseYI C'CCAG_C
Cuts at: 2134 2142 2596 2822 3018 4154 6182 6678 2134
Size: 8 454 226 196 1136 2028 496 2435
Fragments arranged by size:
2435 2028 1136 496 454 226 196 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2696 1163
Size: 573 960 5446
Fragments arranged by size:
5446 960 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2105 4429 5190 6218 1540
Size: 275 186 104 2324 761 1028 2301
Fragments arranged by size:
2324 2301 1028 761 275 186 104
BsiHKAI G_wGCw'C
Cuts at: 2192 2200 2835 3236 3905 5040 5125 6196 2192
Size: 8 635 401 669 1135 85 1071 2975
Fragments arranged by size:
2975 1135 1071 669 635 401 85 8
BsmI GAATG_Cn'
Cuts at: 4490 4583 4490
Size: 93 6886
BsmBI CGTCTCn'nnnn_
Cuts at: 2692 2692
Size: 6979
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2150 2192 2200 2835 3000 3236 3905
Size: 399 360 42 8 635 165 236 669
Cuts at: 3905 4033 4161 4298 5040 5125 6196 1391
Size: 128 128 137 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 669 635 399 360 236
165 137 128 128 85 42 8
BspEI T'CCGG_A
Cuts at: 3522 6642 3522
Size: 3120 3859
BspHI T'CATG_A
Cuts at: 4868 6598 4868
Size: 1730 5249
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2082 2668 3241 3330 3547 4334 844
Size: 1238 586 573 89 217 787 3489
Fragments arranged by size:
3489 1238 787 586 573 217 89
BsrBI CCG'CTC
Cuts at: 1394 1823 3636 4866 1394
Size: 429 1813 1230 3507
Fragments arranged by size:
3507 1813 1230 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2073 3075 5474 5648 65
Size: 1183 825 1002 2399 174 1396
Fragments arranged by size:
2399 1396 1183 1002 825 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5623 5810 1700
Size: 110 3813 187 2869
Fragments arranged by size:
3813 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4110 96
Size: 4014 2965
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6979
BssSI C'ACGA_G
Cuts at: 2834 3035 3512 5039 6051 2834
Size: 201 477 1527 1012 3762
Fragments arranged by size:
3762 1527 1012 477 201
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2875 794
Size: 2081 4898
BstBI TT'CG_AA
Cuts at: 5783 5783
Size: 6979
BstEII G'GTnAC_C
Cuts at: 1184 2244 2279 2623 3439 4267 1184
Size: 1060 35 344 816 828 3896
Fragments arranged by size:
3896 1060 828 816 344 35
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 3061 3088 2011
Size: 1050 27 5902
Fragments arranged by size:
5902 1050 27
BstYI r'GATC_y
Cuts at: 1972 3096 3421 4309 4676 5061 5078 6530 6616
Size: 1124 325 888 367 385 17 1452 86
Cuts at: 6616 6628 6974 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1124 888 385 367 346 325
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3107 3107
Size: 6979
BtgI C'CryG_G
Cuts at: 513 1065 2004 2433 2802 513
Size: 552 939 429 369 4690
Fragments arranged by size:
4690 939 552 429 369
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3040 487
Size: 683 56 1814 4426
Fragments arranged by size:
4426 1814 683 56
BtsI GCAGTG_nn'
Cuts at: 2045 2192 2476 2808 4495 5289 5309 2045
Size: 147 284 332 1687 794 20 3715
Fragments arranged by size:
3715 1687 794 332 284 147 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6979
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3018 3053 529
Size: 35 2454 35 4455
Fragments arranged by size:
4455 2454 35 35
DraI TTT'AAA
Cuts at: 4283 4283
Size: 6979
DraIII CAC_nnn'GTG
Cuts at: 2567 4053 2567
Size: 1486 5493
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5986 809
Size: 507 4670 1802
Fragments arranged by size:
4670 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2102 2304 3450
Size: 54 1425 50 275 18 272 202 1146
Cuts at: 3450 3642 4426 5315 6944 8
Size: 192 784 889 1629 43
Fragments arranged by size:
1629 1425 1146 889 784 275 272 202
192 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2102 4426 1537
Size: 275 290 2324 4090
Fragments arranged by size:
4090 2324 290 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2588 3274 3317 4909 1353
Size: 1235 686 43 1592 3423
Fragments arranged by size:
3423 1592 1235 686 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2030 2188 3434 5566 5940 6086
Size: 1064 138 441 158 1246 2132 374 146
Cuts at: 6086 387
Size: 1280
Fragments arranged by size:
2132 1280 1246 1064 441 374 158 146
138
EcoICRI GAG'CTC
Cuts at: 3903 3903
Size: 6979
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2783 3279 3383 3707 3782 3788 3995
Size: 393 1151 496 104 324 75 6 207
Cuts at: 3995 4088 5042 5639 6426 6694 1239
Size: 93 954 597 787 268 1524
Fragments arranged by size:
1524 1151 954 787 597 496 393 324
268 207 104 93 75 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2585 3224 3326 3404 3554 2585
Size: 639 102 78 150 6010
Fragments arranged by size:
6010 639 150 102 78
EcoO109I rG'GnC_Cy
Cuts at: 2160 2439 3955 3979 2160
Size: 279 1516 24 5160
Fragments arranged by size:
5160 1516 279 24
EcoRI G'AATT_C
Cuts at: 4288 4666 4288
Size: 378 6601
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4210 4210
Size: 6979
FspI TGC'GCA
Cuts at: 5485 5485
Size: 6979
HaeII r_GCGC'y
Cuts at: 1678 2143 3048 3825 6126 1678
Size: 465 905 777 2301 2531
Fragments arranged by size:
2531 2301 905 777 465
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2273 2305 3380 3412 5620
Size: 32 135 32 701 32 1075 32 2208
Cuts at: 5620 5652 5694 5726 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2208 1075 701 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3712 3844 4323 4569 669
Size: 988 281 1774 132 479 246 3079
Fragments arranged by size:
3079 1774 988 479 281 246 132
HindIII A'AGCT_T
Cuts at: 748 3791 748
Size: 3043 3936
HpaI GTT'AAC
Cuts at: 4569 4569
Size: 6979
KasI G'GCGC_C
Cuts at: 3821 3821
Size: 6979
KpnI G_GTAC'C
Cuts at: 4304 4304
Size: 6979
MfeI C'AATT_G
Cuts at: 4578 4578
Size: 6979
MluI A'CGCG_T
Cuts at: 6855 6855
Size: 6979
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2245 4255 6093 6277 1305
Size: 940 2010 1838 184 2007
Fragments arranged by size:
2010 2007 1838 940 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2306 10
Size: 54 1425 343 474 4683
Fragments arranged by size:
4683 1425 474 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2432 2456 3059 3353 4937 5296 5455
Size: 648 1266 24 603 294 1584 359 159
Cuts at: 5455 518
Size: 2042
Fragments arranged by size:
2042 1584 1266 648 603 359 294 159
24
MspA1I CmG'CkG
Cuts at: 1606 2107 2666 2672 2816 2875 3022 3760 4395
Size: 501 559 6 144 59 147 738 635
Cuts at: 4395 5072 6220 6465 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 738 677 635 559 501 245
147 144 59 6
NarI GG'CG_CC
Cuts at: 3822 3822
Size: 6979
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5488
Fragments arranged by size:
5488 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6979
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6979
NotI GC'GGCC_GC
Cuts at: 4426 4426
Size: 6979
NsiI A_TGCA'T
Cuts at: 4174 4174
Size: 6979
NspI r_CATG'y
Cuts at: 1132 1229 3373 3394 4337 5882 1132
Size: 97 2144 21 943 1545 2229
Fragments arranged by size:
2229 2144 1545 943 97 21
PasI CC'CwG_GG
Cuts at: 1348 3151 3982 1348
Size: 1803 831 4345
Fragments arranged by size:
4345 1803 831
PciI A'CATG_T
Cuts at: 5878 5878
Size: 6979
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3142 1108
Size: 2034 4945
PfoI T'CCnGG_A
Cuts at: 4017 4089 4017
Size: 72 6907
PmeI GTTT'AAAC
Cuts at: 4283 4283
Size: 6979
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5048 5080 6561 6593 5048
Size: 32 1481 32 5434
Fragments arranged by size:
5434 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2160 2439 2160
Size: 279 6700
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6979
PsiI TTA'TAA
Cuts at: 4549 4549
Size: 6979
PstI C_TGCA'G
Cuts at: 830 2671 2815 2868 2989 3780 4331 830
Size: 1841 144 53 121 791 551 3478
Fragments arranged by size:
3478 1841 791 551 144 121 53
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6979
PvuII CAG'CTG
Cuts at: 2666 2672 2816 2875 3022 3760 2666
Size: 6 144 59 147 738 5885
Fragments arranged by size:
5885 738 147 144 59 6
SacI G_AGCT'C
Cuts at: 3905 3905
Size: 6979
SalI G'TCGA_C
Cuts at: 1936 3710 4321 1936
Size: 1774 611 4594
Fragments arranged by size:
4594 1774 611
SbfI CC_TGCA'GG
Cuts at: 2989 4331 2989
Size: 1342 5637
ScaI AGT'ACT
Cuts at: 3596 5227 6939 3596
Size: 1631 1712 3636
Fragments arranged by size:
3636 1712 1631
SexAI A'CCwGG_T
Cuts at: 2177 2177
Size: 6979
SfcI C'TryA_G
Cuts at: 826 1045 2374 2667 2811 2864 2985 3673 3776
Size: 219 1329 293 144 53 121 688 103
Cuts at: 3776 4327 5462 6143 6334 826
Size: 551 1135 681 191 1471
Fragments arranged by size:
1471 1329 1135 688 681 551 293 219
191 144 121 103 53
SfiI GGCCn_nnn'nGGCC
Cuts at: 2495 2495
Size: 6979
SfoI GGC'GCC
Cuts at: 3823 3823
Size: 6979
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6979
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6979
SmaI CCC'GGG
Cuts at: 1872 2543 2893 4306 1872
Size: 671 350 1413 4545
Fragments arranged by size:
4545 1413 671 350
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2696 3743 4058 4148 5083 5984
Size: 197 926 753 1047 315 90 935 901
Cuts at: 5984 6246 820
Size: 262 1553
Fragments arranged by size:
1553 1047 935 926 901 753 315 262
197 90
SnaBI TAC'GTA
Cuts at: 493 4770 493
Size: 4277 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6979
SphI G_CATG'C
Cuts at: 1132 3373 4337 1132
Size: 2241 964 3774
Fragments arranged by size:
3774 2241 964
SspI AAT'ATT
Cuts at: 5 52 4075 4903 5
Size: 47 4023 828 2081
Fragments arranged by size:
4023 2081 828 47
StuI AGG'CCT
Cuts at: 1517 2498 4243 1517
Size: 981 1745 4253
Fragments arranged by size:
4253 1745 981
StyI C'CwwG_G
Cuts at: 513 1065 2004 2156 3327 3475 3606 3816 3951
Size: 552 939 152 1171 148 131 210 135
Cuts at: 3951 4418 513
Size: 467 3074
Fragments arranged by size:
3074 1171 939 552 467 210 152 148
135 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2044 2267 3032 4016 4255 5006 5023
Size: 1255 464 223 765 984 239 751 17
Cuts at: 5023 5176 5361 5806 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 984 765 751 464 445 239
223 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3594 3651 4110
Size: 274 80 33 51 1443 1617 57 459
Cuts at: 4110 5225 6937 96
Size: 1115 1712 138
Fragments arranged by size:
1712 1617 1443 1115 459 274 138 80
57 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2553 2585 4037 4069 6939 6971 2553
Size: 32 1452 32 2870 32 2561
Fragments arranged by size:
2870 2561 1452 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6979
XbaI T'CTAG_A
Cuts at: 4315 4315
Size: 6979
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2792 3334 4151 2792
Size: 542 817 5620
Fragments arranged by size:
5620 817 542
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6979
XmaI C'CCGG_G
Cuts at: 1870 2541 2891 4304 1870
Size: 671 350 1413 4545
Fragments arranged by size:
4545 1413 671 350
XmnI GAAnn'nnTTC
Cuts at: 3098 3629 4076 5108 3098
Size: 531 447 1032 4969
Fragments arranged by size:
4969 1032 531 447
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4787 276
Size: 53 83 186 936 3253 2468
Fragments arranged by size:
3253 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BplI BsiWI BstZ17I EcoRV FseI
FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SrfI SwaI