Restriction map for
pFN21AE6701
(Circular) (Six-base) MAPSORT of: pFN21AE6701.seq Check: 9480 from: 1 to: 6628
pFN21AE6701 6628 bp
With 182 enzymes: SgfI *
March 13, 2015 14:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4438 278
Size: 53 83 186 936 2902 2468
Fragments arranged by size:
2902 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3971 1937
Size: 2034 4594
Acc65I G'GTAC_C
Cuts at: 3949 3949
Size: 6628
AclI AA'CG_TT
Cuts at: 1563 4755 5128 1563
Size: 3192 373 3063
Fragments arranged by size:
3192 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2039 2072 2421 2771 2803 2922 3410 3489
Size: 407 33 349 350 32 119 488 79
Cuts at: 3489 3848 4691 6075 6343 1632
Size: 359 843 1384 268 1917
Fragments arranged by size:
1917 1384 843 488 407 359 350 349
268 119 79 33 32
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6431
AflIII A'CryG_T
Cuts at: 1203 5527 6504 1203
Size: 4324 977 1327
Fragments arranged by size:
4324 1327 977
AgeI A'CCGG_T
Cuts at: 5459 5459
Size: 6628
AhdI GACnn_n'nnGTC
Cuts at: 5357 5357
Size: 6628
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6628
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2937 3422 1184
Size: 1753 485 4390
Fragments arranged by size:
4390 1753 485
AlwNI CAG_nnn'CTG
Cuts at: 2050 2917 3816 4371 5943 2050
Size: 867 899 555 1572 2735
Fragments arranged by size:
2735 1572 899 867 555
ApaLI G'TGCA_C
Cuts at: 4685 5841 4685
Size: 1156 5472
ApoI r'AATT_y
Cuts at: 1493 2787 2895 3302 3764 3937 4163 4315 6424
Size: 1294 108 407 462 173 226 152 2109
Cuts at: 6424 1493
Size: 1697
Fragments arranged by size:
2109 1697 1294 462 407 226 173 152
108
AseI AT'TA_AT
Cuts at: 160 1032 5182 160
Size: 872 4150 1606
Fragments arranged by size:
4150 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6628
AvaI C'yCGr_G
Cuts at: 1870 1943 3953 1870
Size: 73 2010 4545
Fragments arranged by size:
4545 2010 73
BamHI G'GATC_C
Cuts at: 3958 4325 3958
Size: 367 6261
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3949 5404 618
Size: 325 217 627 2162 1455 1842
Fragments arranged by size:
2162 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3279 3927 3947 1391
Size: 1888 648 20 4072
Fragments arranged by size:
4072 1888 648 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2440 2809 2872 3253 928
Size: 971 541 369 63 381 4303
Fragments arranged by size:
4303 971 541 381 369 63
BbvCI CC'TCA_GC
Cuts at: 3579 3579
Size: 6628
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4819 4853 1903
Size: 34 2882 34 3678
Fragments arranged by size:
3678 2882 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4520 5730 4520
Size: 1210 5418
BclI T'GATC_A
Cuts at: 1548 3772 1548
Size: 2224 4404
BfrBI ATG'CAT
Cuts at: 2064 2064
Size: 6628
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5239 136
Size: 107 122 71 631 605 3567 1525
Fragments arranged by size:
3567 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2440 6623 2440
Size: 4183 2445
BlpI GC'TnA_GC
Cuts at: 4045 4045
Size: 6628
Bme1580I G_kGCm'C
Cuts at: 1790 2244 2579 3192 4689 5845 1790
Size: 454 335 613 1497 1156 2573
Fragments arranged by size:
2573 1497 1156 613 454 335
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6628
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2246 3154 4359
Size: 416 53 83 399 24 829 908 1205
Cuts at: 4359 5317 442
Size: 958 1753
Fragments arranged by size:
1753 1205 958 908 829 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6628
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2908 3608 5288 1239
Size: 1669 700 1680 2579
Fragments arranged by size:
2579 1680 1669 700
Bpu10I CC'TnA_GC
Cuts at: 3115 3485 3579 3115
Size: 370 94 6164
Fragments arranged by size:
6164 370 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4753 5618 5916 4753
Size: 865 298 5465
Fragments arranged by size:
5465 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5291 882
Size: 617 3792 2219
Fragments arranged by size:
3792 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4419 493
Size: 711 3215 2702
Fragments arranged by size:
3215 2702 711
BsaBI GATnn'nnATC
Cuts at: 3096 6622 3096
Size: 3526 3102
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4435 4817 5452 275
Size: 53 83 186 936 2902 382 635 1451
Fragments arranged by size:
2902 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5061 5459 5733 5880 6291 5061
Size: 398 274 147 411 5398
Fragments arranged by size:
5398 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2430 2460 3581 3611 1178
Size: 30 165 30 1027 30 1121 30 4195
Fragments arranged by size:
4195 1121 1027 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2004 2645 2732 1186
Size: 818 641 87 5082
Fragments arranged by size:
5082 818 641 87
BseYI C'CCAG_C
Cuts at: 2357 3338 3809 5831 6327 2357
Size: 981 471 2022 496 2658
Fragments arranged by size:
2658 2022 981 496 471
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6055
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4078 4839 5867 1540
Size: 275 186 2077 761 1028 2301
Fragments arranged by size:
2301 2077 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3279 3927 4689 4774 5845 3279
Size: 648 762 85 1071 4062
Fragments arranged by size:
4062 1071 762 648 85
BsmI GAATG_Cn'
Cuts at: 2066 2488 4139 4232 2066
Size: 422 1651 93 4462
Fragments arranged by size:
4462 1651 422 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2244 2579 3192 3279 3927 3947 4689
Size: 399 454 335 613 87 648 20 742
Cuts at: 4689 4774 5845 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 648 613 454 399 335
87 85 20
BspEI T'CCGG_A
Cuts at: 6291 6291
Size: 6628
BspHI T'CATG_A
Cuts at: 4517 6247 4517
Size: 1730 4898
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3231 3778 3849 3983 844
Size: 2387 547 71 134 3489
Fragments arranged by size:
3489 2387 547 134 71
BsrBI CCG'CTC
Cuts at: 1394 1823 3114 4515 1394
Size: 429 1291 1401 3507
Fragments arranged by size:
3507 1401 1291 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3034 5123 5297 65
Size: 1183 1786 2089 174 1396
Fragments arranged by size:
2089 1786 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5272 5459 1700
Size: 110 3462 187 2869
Fragments arranged by size:
3462 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2658 96
Size: 2562 4066
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6628
BssSI C'ACGA_G
Cuts at: 3534 4688 5700 3534
Size: 1154 1012 4462
Fragments arranged by size:
4462 1154 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6628
BstBI TT'CG_AA
Cuts at: 5432 5432
Size: 6628
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6628
BstYI r'GATC_y
Cuts at: 1972 2440 3958 4325 4710 4727 6179 6265 6277
Size: 468 1518 367 385 17 1452 86 12
Cuts at: 6277 6623 1972
Size: 346 1977
Fragments arranged by size:
1977 1518 1452 468 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3180 3180
Size: 6628
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5137
Fragments arranged by size:
5137 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3924 487
Size: 683 56 2698 3191
Fragments arranged by size:
3191 2698 683 56
BtsI GCAGTG_nn'
Cuts at: 3206 4144 4938 4958 3206
Size: 938 794 20 4876
Fragments arranged by size:
4876 938 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6628
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3337 3372 529
Size: 35 2773 35 3785
Fragments arranged by size:
3785 2773 35 35
DraI TTT'AAA
Cuts at: 3932 3932
Size: 6628
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5635 809
Size: 507 4319 1802
Fragments arranged by size:
4319 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3049 4075 4964
Size: 54 1425 50 275 18 1219 1026 889
Cuts at: 4964 6593 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1219 1026 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4075 1537
Size: 275 2263 4090
Fragments arranged by size:
4090 2263 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2030 2898 3284 4558 1353
Size: 677 868 386 1274 3423
Fragments arranged by size:
3423 1274 868 677 386
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3296 5215 5589 5735 387
Size: 1064 138 1707 1919 374 146 1280
Fragments arranged by size:
1919 1707 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3277 3925 3277
Size: 648 5980
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2072 2421 2771 2803 2908 2922
Size: 393 407 33 349 350 32 105 14
Cuts at: 2922 3410 3489 3608 3848 4691 5288 6075 6343
Size: 488 79 119 240 843 597 787 268
Cuts at: 6343 1239
Size: 1524
Fragments arranged by size:
1524 843 787 597 488 407 393 350
349 268 240 119 105 79 33 32
14
EcoNI CCTnn'n_nnAGG
Cuts at: 3392 3392
Size: 6628
EcoO109I rG'GnC_Cy
Cuts at: 2009 2332 2530 3220 3583 2009
Size: 323 198 690 363 5054
Fragments arranged by size:
5054 690 363 323 198
EcoRI G'AATT_C
Cuts at: 3937 4315 3937
Size: 378 6250
FspI TGC'GCA
Cuts at: 3227 5134 3227
Size: 1907 4721
HaeII r_GCGC'y
Cuts at: 1678 5775 1678
Size: 4097 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2430 2462 3050 3082 3815
Size: 32 135 32 858 32 588 32 733
Cuts at: 3815 3847 5269 5301 5343 5375 1373
Size: 32 1422 32 42 32 2626
Fragments arranged by size:
2626 1422 858 733 588 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2596 3972 4218 669
Size: 988 281 658 1376 246 3079
Fragments arranged by size:
3079 1376 988 658 281 246
HindIII A'AGCT_T
Cuts at: 748 2499 748
Size: 1751 4877
HpaI GTT'AAC
Cuts at: 4218 4218
Size: 6628
KpnI G_GTAC'C
Cuts at: 3953 3953
Size: 6628
MfeI C'AATT_G
Cuts at: 4227 4227
Size: 6628
MluI A'CGCG_T
Cuts at: 6504 6504
Size: 6628
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2117 3884 5742 5926 1305
Size: 812 1767 1858 184 2007
Fragments arranged by size:
2007 1858 1767 812 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4806
Fragments arranged by size:
4806 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2465 4586 4945 5104 518
Size: 648 1299 2121 359 159 2042
Fragments arranged by size:
2121 2042 1299 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2221 4044 4721 5869 6114 1606
Size: 615 1823 677 1148 245 2120
Fragments arranged by size:
2120 1823 1148 677 615 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5137
Fragments arranged by size:
5137 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6628
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6628
NotI GC'GGCC_GC
Cuts at: 4075 4075
Size: 6628
NsiI A_TGCA'T
Cuts at: 2066 2066
Size: 6628
NspI r_CATG'y
Cuts at: 1132 1229 2602 3508 3986 5531 1132
Size: 97 1373 906 478 1545 2229
Fragments arranged by size:
2229 1545 1373 906 478 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6628
PciI A'CATG_T
Cuts at: 5527 5527
Size: 6628
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6628
PfoI T'CCnGG_A
Cuts at: 2140 3327 2140
Size: 1187 5441
PmeI GTTT'AAAC
Cuts at: 3932 3932
Size: 6628
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4697 4729 6210 6242 4697
Size: 32 1481 32 5083
Fragments arranged by size:
5083 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2009 2332 3220 3583 2009
Size: 323 888 363 5054
Fragments arranged by size:
5054 888 363 323
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6628
PsiI TTA'TAA
Cuts at: 4198 4198
Size: 6628
PstI C_TGCA'G
Cuts at: 830 3153 3213 3980 830
Size: 2323 60 767 3478
Fragments arranged by size:
3478 2323 767 60
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6628
PvuII CAG'CTG
Cuts at: 2221 2221
Size: 6628
SacI G_AGCT'C
Cuts at: 3279 3927 3279
Size: 648 5980
SalI G'TCGA_C
Cuts at: 1936 3970 1936
Size: 2034 4594
SanDI GG'GwC_CC
Cuts at: 2332 2332
Size: 6628
SapI GCTCTTCn'nnn_
Cuts at: 2030 3284 2030
Size: 1254 5374
SbfI CC_TGCA'GG
Cuts at: 3980 3980
Size: 6628
ScaI AGT'ACT
Cuts at: 4876 6588 4876
Size: 1712 4916
SfcI C'TryA_G
Cuts at: 826 1045 3149 3209 3712 3976 5111 5792 5983
Size: 219 2104 60 503 264 1135 681 191
Cuts at: 5983 826
Size: 1471
Fragments arranged by size:
2104 1471 1135 681 503 264 219 191
60
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6628
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6628
SmaI CCC'GGG
Cuts at: 1872 3955 1872
Size: 2083 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4732 5633 5895 820
Size: 197 926 2789 901 262 1553
Fragments arranged by size:
2789 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4419 493
Size: 3926 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6628
SphI G_CATG'C
Cuts at: 1132 3986 1132
Size: 2854 3774
SspI AAT'ATT
Cuts at: 5 52 4552 5
Size: 47 4500 2081
Fragments arranged by size:
4500 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6628
StyI C'CwwG_G
Cuts at: 513 1065 2004 2227 2859 4067 513
Size: 552 939 223 632 1208 3074
Fragments arranged by size:
3074 1208 939 632 552 223
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3713 4655 4672 4825 5010 5455 325
Size: 1255 2133 942 17 153 185 445 1498
Fragments arranged by size:
2133 1498 1255 942 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2637 2658 3162
Size: 274 80 33 51 1443 660 21 504
Cuts at: 3162 3742 4874 6586 96
Size: 580 1132 1712 138
Fragments arranged by size:
1712 1443 1132 660 580 504 274 138
80 51 33 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2136 2168 3715 3747 6588 6620 2136
Size: 32 1547 32 2841 32 2144
Fragments arranged by size:
2841 2144 1547 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6628
XbaI T'CTAG_A
Cuts at: 3964 3964
Size: 6628
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3335 3335
Size: 6628
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6628
XmaI C'CCGG_G
Cuts at: 1870 3953 1870
Size: 2083 4545
XmnI GAAnn'nnTTC
Cuts at: 2024 4757 2024
Size: 2733 3895
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4436 276
Size: 53 83 186 936 2902 2468
Fragments arranged by size:
2902 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI
SfoI SrfI SwaI