Restriction map for
pFN21AE6696
(Circular) (Six-base) MAPSORT of: pFN21AE6696.seq Check: 5898 from: 1 to: 5905
pFN21AE6696 5905 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2853 3715 278
Size: 53 83 186 936 1317 862 2468
Fragments arranged by size:
2468 1317 936 862 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3248 1937
Size: 1311 4594
Acc65I G'GTAC_C
Cuts at: 3226 3226
Size: 5905
AclI AA'CG_TT
Cuts at: 1563 4032 4405 1563
Size: 2469 373 3063
Fragments arranged by size:
3063 2469 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2151 2732 3968 5352 5620 1632
Size: 519 581 1236 1384 268 1917
Fragments arranged by size:
1917 1384 1236 581 519 268
AfeI AGC'GCT
Cuts at: 3149 3149
Size: 5905
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5708
AflIII A'CryG_T
Cuts at: 1203 2064 2924 4804 5781 1203
Size: 861 860 1880 977 1327
Fragments arranged by size:
1880 1327 977 861 860
AgeI A'CCGG_T
Cuts at: 4736 4736
Size: 5905
AhdI GACnn_n'nnGTC
Cuts at: 4634 4634
Size: 5905
AleI CACnn'nnGTG
Cuts at: 1166 3111 1166
Size: 1945 3960
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5905
AlwNI CAG_nnn'CTG
Cuts at: 2129 2746 3648 5220 2129
Size: 617 902 1572 2814
Fragments arranged by size:
2814 1572 902 617
ApaI G_GGCC'C
Cuts at: 2079 2079
Size: 5905
ApaLI G'TGCA_C
Cuts at: 3962 5118 3962
Size: 1156 4749
ApoI r'AATT_y
Cuts at: 1493 2954 3214 3440 3592 5701 1493
Size: 1461 260 226 152 2109 1697
Fragments arranged by size:
2109 1697 1461 260 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4459 160
Size: 872 3427 1606
Fragments arranged by size:
3427 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
AvaI C'yCGr_G
Cuts at: 1870 1943 2072 2115 2642 3087 3230 1870
Size: 73 129 43 527 445 143 4545
Fragments arranged by size:
4545 527 445 143 129 73 43
AvrII C'CTAG_G
Cuts at: 2272 2272
Size: 5905
BamHI G'GATC_C
Cuts at: 3235 3602 3235
Size: 367 5538
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2796 3083 3226 4681 618
Size: 325 217 627 1009 287 143 1455 1842
Fragments arranged by size:
1842 1455 1009 627 325 287 217 143
BanII G_rGCy'C
Cuts at: 1391 2079 2988 3193 3224 1391
Size: 688 909 205 31 4072
Fragments arranged by size:
4072 909 688 205 31
BbeI G_GCGC'C
Cuts at: 2800 2800
Size: 5905
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2447 2945 928
Size: 971 548 498 3888
Fragments arranged by size:
3888 971 548 498
BbvCI CC'TCA_GC
Cuts at: 2690 3199 2690
Size: 509 5396
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4096 4130 1903
Size: 34 2159 34 3678
Fragments arranged by size:
3678 2159 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3797 5007 3797
Size: 1210 4695
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5905
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2019 2083 2420
Size: 107 122 71 631 605 347 64 337
Cuts at: 2420 2837 4516 136
Size: 417 1679 1525
Fragments arranged by size:
1679 1525 631 605 417 347 337 122
107 71 64
BglII A'GATC_T
Cuts at: 2097 5900 2097
Size: 3803 2102
BlpI GC'TnA_GC
Cuts at: 3322 3322
Size: 5905
Bme1580I G_kGCm'C
Cuts at: 1790 2072 2079 2595 3088 3966 5122 1790
Size: 282 7 516 493 878 1156 2573
Fragments arranged by size:
2573 1156 878 516 493 282 7
BmgBI CAC'GTC
Cuts at: 1318 2925 1318
Size: 1607 4298
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3636 4594 442
Size: 416 53 83 399 24 2219 958 1753
Fragments arranged by size:
2219 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2303 2430 2771 3130 3164 4565 1239
Size: 1064 127 341 359 34 1401 2579
Fragments arranged by size:
2579 1401 1064 359 341 127 34
Bpu10I CC'TnA_GC
Cuts at: 2528 2690 3199 2528
Size: 162 509 5234
Fragments arranged by size:
5234 509 162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2336 2591 4030 4895 5193 2336
Size: 255 1439 865 298 3048
Fragments arranged by size:
3048 1439 865 298 255
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4568 882
Size: 617 3069 2219
Fragments arranged by size:
3069 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3696 493
Size: 711 2492 2702
Fragments arranged by size:
2702 2492 711
BsaBI GATnn'nnATC
Cuts at: 5899 5899
Size: 5905
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2663 2797 2850 3712
Size: 53 83 186 936 1130 134 53 862
Cuts at: 3712 4094 4729 275
Size: 382 635 1451
Fragments arranged by size:
1451 1130 936 862 635 382 186 134
83 53 53
BsaWI w'CCGG_w
Cuts at: 4338 4736 5010 5157 5568 4338
Size: 398 274 147 411 4675
Fragments arranged by size:
4675 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2294 2324 1178
Size: 30 165 30 891 30 4759
Fragments arranged by size:
4759 891 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2768 2978 1186
Size: 1582 210 4113
Fragments arranged by size:
4113 1582 210
BseYI C'CCAG_C
Cuts at: 5108 5604 5108
Size: 496 5409
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2299 2306 2381 1163
Size: 573 563 7 75 4687
Fragments arranged by size:
4687 573 563 75 7
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2026 2305 3186 3355 4116 5144
Size: 275 186 25 279 881 169 761 1028
Cuts at: 5144 1540
Size: 2301
Fragments arranged by size:
2301 1028 881 761 279 275 186 169
25
BsiHKAI G_wGCw'C
Cuts at: 2052 2529 2988 3966 4051 5122 2052
Size: 477 459 978 85 1071 2835
Fragments arranged by size:
2835 1071 978 477 459 85
BsmI GAATG_Cn'
Cuts at: 3416 3509 3416
Size: 93 5812
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2072 2079 2529 2595 2988 3088
Size: 399 262 20 7 450 66 393 100
Cuts at: 3088 3193 3224 3966 4051 5122 1391
Size: 105 31 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 450 399 393 262 105
100 85 66 31 20 7
BspEI T'CCGG_A
Cuts at: 5568 5568
Size: 5905
BspHI T'CATG_A
Cuts at: 3794 5524 3794
Size: 1730 4175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3260 844
Size: 2416 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2148 3792 1394
Size: 429 325 1644 3507
Fragments arranged by size:
3507 1644 429 325
BsrDI GCAATG_nn'
Cuts at: 65 1248 4400 4574 65
Size: 1183 3152 174 1396
Fragments arranged by size:
3152 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4549 4736 1700
Size: 110 2739 187 2869
Fragments arranged by size:
2869 2739 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5905
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5905
BssSI C'ACGA_G
Cuts at: 3965 4977 3965
Size: 1012 4893
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2404 2524 2746 794
Size: 1610 120 222 3953
Fragments arranged by size:
3953 1610 222 120
BstBI TT'CG_AA
Cuts at: 4709 4709
Size: 5905
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5905
BstXI CCAn_nnnn'nTGG
Cuts at: 3010 3010
Size: 5905
BstYI r'GATC_y
Cuts at: 1972 2097 2583 3235 3602 3987 4004 5456 5542
Size: 125 486 652 367 385 17 1452 86
Cuts at: 5542 5554 5900 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 652 486 385 367 346 125
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2223 2336 2888 2223
Size: 113 552 5240
Fragments arranged by size:
5240 552 113
BtgI C'CryG_G
Cuts at: 513 1065 2358 2844 513
Size: 552 1293 486 3574
Fragments arranged by size:
3574 1293 552 486
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5166
Fragments arranged by size:
5166 683 56
BtsI GCAGTG_nn'
Cuts at: 3421 4215 4235 3421
Size: 794 20 5091
Fragments arranged by size:
5091 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5905
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5870
DraI TTT'AAA
Cuts at: 3209 3209
Size: 5905
DraIII CAC_nnn'GTG
Cuts at: 2524 2524
Size: 5905
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2387 2873 4912 809
Size: 507 1071 486 2039 1802
Fragments arranged by size:
2039 1802 1071 507 486
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2761 2997
Size: 54 1425 50 275 18 225 706 236
Cuts at: 2997 3183 3352 4241 5870 8
Size: 186 169 889 1629 43
Fragments arranged by size:
1629 1425 889 706 275 236 225 186
169 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3183 3352 1537
Size: 275 1371 169 4090
Fragments arranged by size:
4090 1371 275 169
EarI CTCTTCn'nnn_
Cuts at: 1353 2677 3835 1353
Size: 1324 1158 3423
Fragments arranged by size:
3423 1324 1158
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2081 2588 2744 4492 4866 5012
Size: 1064 138 492 507 156 1748 374 146
Cuts at: 5012 387
Size: 1280
Fragments arranged by size:
1748 1280 1064 507 492 374 156 146
138
EcoICRI GAG'CTC
Cuts at: 2986 2986
Size: 5905
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2151 2303 2430 2732 2771 3130 3164
Size: 393 519 152 127 302 39 359 34
Cuts at: 3164 3968 4565 5352 5620 1239
Size: 804 597 787 268 1524
Fragments arranged by size:
1524 804 787 597 519 393 359 302
268 152 127 39 34
EcoO109I rG'GnC_Cy
Cuts at: 2126 2158 2347 2479 2126
Size: 32 189 132 5552
Fragments arranged by size:
5552 189 132 32
EcoRI G'AATT_C
Cuts at: 3214 3592 3214
Size: 378 5527
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2416 2416
Size: 5905
FspI TGC'GCA
Cuts at: 4411 4411
Size: 5905
HaeII r_GCGC'y
Cuts at: 1678 2650 2690 2800 3151 5052 1678
Size: 972 40 110 351 1901 2531
Fragments arranged by size:
2531 1901 972 351 110 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2294 2326 2894 2926 4546
Size: 32 135 32 722 32 568 32 1620
Cuts at: 4546 4578 4620 4652 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1620 722 568 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2854 3249 3495 669
Size: 988 281 916 395 246 3079
Fragments arranged by size:
3079 988 916 395 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5905
HpaI GTT'AAC
Cuts at: 3495 3495
Size: 5905
KasI G'GCGC_C
Cuts at: 2796 2796
Size: 5905
KpnI G_GTAC'C
Cuts at: 3230 3230
Size: 5905
MfeI C'AATT_G
Cuts at: 3504 3504
Size: 5905
MluI A'CGCG_T
Cuts at: 5781 5781
Size: 5905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2700 5019 5203 1305
Size: 1395 2319 184 2007
Fragments arranged by size:
2319 2007 1395 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2057 2999 10
Size: 54 1425 343 225 942 2916
Fragments arranged by size:
2916 1425 942 343 225 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2063 3111 3863 4222 4381 518
Size: 648 897 1048 752 359 159 2042
Fragments arranged by size:
2042 1048 897 752 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2493 2659 2684 3321 3998 5146 5391 1606
Size: 887 166 25 637 677 1148 245 2120
Fragments arranged by size:
2120 1148 887 677 637 245 166 25
NarI GG'CG_CC
Cuts at: 2797 2797
Size: 5905
NcoI C'CATG_G
Cuts at: 513 1065 2358 2844 513
Size: 552 1293 486 3574
Fragments arranged by size:
3574 1293 552 486
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5905
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5905
NotI GC'GGCC_GC
Cuts at: 3352 3352
Size: 5905
NspI r_CATG'y
Cuts at: 1132 1229 3263 4808 1132
Size: 97 2034 1545 2229
Fragments arranged by size:
2229 2034 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3073 1348
Size: 1725 4180
PciI A'CATG_T
Cuts at: 4804 4804
Size: 5905
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5905
PfoI T'CCnGG_A
Cuts at: 2043 2043
Size: 5905
PmeI GTTT'AAAC
Cuts at: 3209 3209
Size: 5905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3974 4006 5487 5519 3974
Size: 32 1481 32 4360
Fragments arranged by size:
4360 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2126 2479 2126
Size: 353 5552
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5905
PsiI TTA'TAA
Cuts at: 3475 3475
Size: 5905
PspOMI G'GGCC_C
Cuts at: 2075 2075
Size: 5905
PspXI vC'TCGA_Gb
Cuts at: 2642 2642
Size: 5905
PstI C_TGCA'G
Cuts at: 830 2200 3126 3257 830
Size: 1370 926 131 3478
Fragments arranged by size:
3478 1370 926 131
PvuI CG_AT'CG
Cuts at: 2001 2026 2001
Size: 25 5880
SacI G_AGCT'C
Cuts at: 2988 2988
Size: 5905
SalI G'TCGA_C
Cuts at: 1936 3247 1936
Size: 1311 4594
SbfI CC_TGCA'GG
Cuts at: 3257 3257
Size: 5905
ScaI AGT'ACT
Cuts at: 4153 5865 4153
Size: 1712 4193
SfcI C'TryA_G
Cuts at: 826 1045 2196 2326 2785 3122 3253 4388 5069
Size: 219 1151 130 459 337 131 1135 681
Cuts at: 5069 5260 826
Size: 191 1471
Fragments arranged by size:
1471 1151 1135 681 459 337 219 191
131 130
SfiI GGCCn_nnn'nGGCC
Cuts at: 2083 2083
Size: 5905
SfoI GGC'GCC
Cuts at: 2798 2798
Size: 5905
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5905
SmaI CCC'GGG
Cuts at: 1872 2074 2117 3232 1872
Size: 202 43 1115 4545
Fragments arranged by size:
4545 1115 202 43
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2351 2606 2642 4009 4910 5172
Size: 197 926 408 255 36 1367 901 262
Cuts at: 5172 820
Size: 1553
Fragments arranged by size:
1553 1367 926 901 408 262 255 197
36
SnaBI TAC'GTA
Cuts at: 493 3696 493
Size: 3203 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5905
SphI G_CATG'C
Cuts at: 1132 3263 1132
Size: 2131 3774
SspI AAT'ATT
Cuts at: 5 52 3829 5
Size: 47 3777 2081
Fragments arranged by size:
3777 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5905
StyI C'CwwG_G
Cuts at: 513 1065 2206 2272 2343 2358 2844 3344 513
Size: 552 1141 66 71 15 486 500 3074
Fragments arranged by size:
3074 1141 552 500 486 71 66 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3932 3949 4102 4287 4732 325
Size: 1255 2352 17 153 185 445 1498
Fragments arranged by size:
2352 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4151 5863 96
Size: 274 80 33 51 1443 2174 1712 138
Fragments arranged by size:
2174 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2446 2478 5865 5897 2446
Size: 32 3387 32 2454
Fragments arranged by size:
3387 2454 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5905
XbaI T'CTAG_A
Cuts at: 3241 3241
Size: 5905
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3007 3007
Size: 5905
XhoI C'TCGA_G
Cuts at: 1943 2642 1943
Size: 699 5206
XmaI C'CCGG_G
Cuts at: 1870 2072 2115 3230 1870
Size: 202 43 1115 4545
Fragments arranged by size:
4545 1115 202 43
XmnI GAAnn'nnTTC
Cuts at: 4034 4034
Size: 5905
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2851 3713 276
Size: 53 83 186 936 1317 862 2468
Fragments arranged by size:
2468 1317 936 862 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI SacI SalI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BfrBI BplI BsiWI BsmBI
BstZ17I EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PsrI PvuII RsrII SacII SanDI
SapI SexAI SrfI SwaI