Restriction map for
pFN21AE6692
(Circular) (Six-base) MAPSORT of: pFN21AE6692.seq Check: 1770 from: 1 to: 5692
pFN21AE6692 5692 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2766 3502 278
Size: 53 83 186 936 1230 736 2468
Fragments arranged by size:
2468 1230 936 736 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3035 1937
Size: 1098 4594
Acc65I G'GTAC_C
Cuts at: 3013 3013
Size: 5692
AclI AA'CG_TT
Cuts at: 1563 3819 4192 1563
Size: 2256 373 3063
Fragments arranged by size:
3063 2256 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3755 5139 5407 1632
Size: 2123 1384 268 1917
Fragments arranged by size:
2123 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5495
AflIII A'CryG_T
Cuts at: 1203 2271 2730 2934 4591 5568 1203
Size: 1068 459 204 1657 977 1327
Fragments arranged by size:
1657 1327 1068 977 459 204
AgeI A'CCGG_T
Cuts at: 2802 4523 2802
Size: 1721 3971
AhdI GACnn_n'nnGTC
Cuts at: 4421 4421
Size: 5692
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5692
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5692
AlwNI CAG_nnn'CTG
Cuts at: 2830 2851 3435 5007 2830
Size: 21 584 1572 3515
Fragments arranged by size:
3515 1572 584 21
ApaI G_GGCC'C
Cuts at: 2263 2263
Size: 5692
ApaLI G'TGCA_C
Cuts at: 2678 3749 4905 2678
Size: 1071 1156 3465
Fragments arranged by size:
3465 1156 1071
ApoI r'AATT_y
Cuts at: 1493 3001 3227 3379 5488 1493
Size: 1508 226 152 2109 1697
Fragments arranged by size:
2109 1697 1508 226 152
AscI GG'CGCG_CC
Cuts at: 2323 2323
Size: 5692
AseI AT'TA_AT
Cuts at: 160 1032 4246 160
Size: 872 3214 1606
Fragments arranged by size:
3214 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
AvaI C'yCGr_G
Cuts at: 1870 1943 2111 2263 2563 3017 1870
Size: 73 168 152 300 454 4545
Fragments arranged by size:
4545 454 300 168 152 73
BamHI G'GATC_C
Cuts at: 3022 3389 3022
Size: 367 5325
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2309 2741 2752 3013 4468
Size: 325 217 627 522 432 11 261 1455
Cuts at: 4468 618
Size: 1842
Fragments arranged by size:
1842 1455 627 522 432 325 261 217
11
BanII G_rGCy'C
Cuts at: 1391 2112 2263 2529 3011 1391
Size: 721 151 266 482 4072
Fragments arranged by size:
4072 721 482 266 151
BbeI G_GCGC'C
Cuts at: 2313 2745 2756 2313
Size: 432 11 5249
Fragments arranged by size:
5249 432 11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4721
BbvCI CC'TCA_GC
Cuts at: 2693 2693
Size: 5692
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3883 3917 1903
Size: 34 1946 34 3678
Fragments arranged by size:
3678 1946 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3584 4794 3584
Size: 1210 4482
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5692
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2180 2423 2590
Size: 107 122 71 631 605 508 243 167
Cuts at: 2590 2750 4303 136
Size: 160 1553 1525
Fragments arranged by size:
1553 1525 631 605 508 243 167 160
122 107 71
BglII A'GATC_T
Cuts at: 5687 5687
Size: 5692
BlpI GC'TnA_GC
Cuts at: 3109 3109
Size: 5692
Bme1580I G_kGCm'C
Cuts at: 1790 2057 2263 2297 2682 3753 4909 1790
Size: 267 206 34 385 1071 1156 2573
Fragments arranged by size:
2573 1156 1071 385 267 206 34
BmgBI CAC'GTC
Cuts at: 1318 2272 2731 1318
Size: 954 459 4279
Fragments arranged by size:
4279 954 459
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2560 3423 4381
Size: 416 53 83 399 24 1143 863 958
Cuts at: 4381 442
Size: 1753
Fragments arranged by size:
1753 1143 958 863 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2351 2873 2885 4352 1239
Size: 1112 522 12 1467 2579
Fragments arranged by size:
2579 1467 1112 522 12
Bpu10I CC'TnA_GC
Cuts at: 2693 2693
Size: 5692
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2447 3817 4682 4980 2447
Size: 1370 865 298 3159
Fragments arranged by size:
3159 1370 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4355 882
Size: 617 2856 2219
Fragments arranged by size:
2856 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2241 2443 2659 2935 3483 493
Size: 711 1037 202 216 276 548 2702
Fragments arranged by size:
2702 1037 711 548 276 216 202
BsaBI GATnn'nnATC
Cuts at: 5686 5686
Size: 5692
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2310 2385 2742 2753
Size: 53 83 186 936 777 75 357 11
Cuts at: 2753 2763 3499 3881 4516 275
Size: 10 736 382 635 1451
Fragments arranged by size:
1451 936 777 736 635 382 357 186
83 75 53 11 10
BsaWI w'CCGG_w
Cuts at: 2802 4125 4523 4797 4944 5355 2802
Size: 1323 398 274 147 411 3139
Fragments arranged by size:
3139 1323 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2462 2492 1178
Size: 30 165 30 1059 30 4378
Fragments arranged by size:
4378 1059 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2330 1186
Size: 1144 4548
BseYI C'CCAG_C
Cuts at: 2644 4895 5391 2644
Size: 2251 496 2945
Fragments arranged by size:
2945 2251 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2936 1163
Size: 573 1200 3919
Fragments arranged by size:
3919 1200 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2106 2301 2575 2670 3142 3903
Size: 275 186 105 195 274 95 472 761
Cuts at: 3903 4931 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 472 275 274 195 186
105 95
BsiHKAI G_wGCw'C
Cuts at: 2442 2463 2664 2682 2778 3753 3838 4909 2442
Size: 21 201 18 96 975 85 1071 3225
Fragments arranged by size:
3225 1071 975 201 96 85 21 18
BsmI GAATG_Cn'
Cuts at: 3203 3296 3203
Size: 93 5599
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 2112 2263 2297 2442 2463 2529
Size: 399 267 55 151 34 145 21 66
Cuts at: 2529 2664 2682 2778 3011 3753 3838 4909 1391
Size: 135 18 96 233 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 267 233 151 145
135 96 85 66 55 34 21 18
BspEI T'CCGG_A
Cuts at: 5355 5355
Size: 5692
BspHI T'CATG_A
Cuts at: 3581 5311 3581
Size: 1730 3962
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2367 2640 2694 2715 3047 844
Size: 1523 273 54 21 332 3489
Fragments arranged by size:
3489 1523 332 273 54 21
BsrBI CCG'CTC
Cuts at: 1394 1823 2320 2335 3579 1394
Size: 429 497 15 1244 3507
Fragments arranged by size:
3507 1244 497 429 15
BsrDI GCAATG_nn'
Cuts at: 65 1248 4187 4361 65
Size: 1183 2939 174 1396
Fragments arranged by size:
2939 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2300 2755 2802 4336 4523 1700
Size: 110 490 455 47 1534 187 2869
Fragments arranged by size:
2869 1534 490 455 187 110 47
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5692
BssHII G'CGCG_C
Cuts at: 1925 2093 2162 2164 2199 2279 2323 2515 2531
Size: 168 69 2 35 80 44 192 16
Cuts at: 2531 2710 2885 1925
Size: 179 175 4732
Fragments arranged by size:
4732 192 179 175 168 80 69 44
35 16 2
BssSI C'ACGA_G
Cuts at: 3752 4764 3752
Size: 1012 4680
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2686 794
Size: 1892 3800
BstBI TT'CG_AA
Cuts at: 4496 4496
Size: 5692
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5692
BstXI CCAn_nnnn'nTGG
Cuts at: 2413 2413
Size: 5692
BstYI r'GATC_y
Cuts at: 1972 3022 3389 3774 3791 5243 5329 5341 5687
Size: 1050 367 385 17 1452 86 12 346
Cuts at: 5687 1972
Size: 1977
Fragments arranged by size:
1977 1452 1050 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2289 2569 2809 513
Size: 552 1224 280 240 3396
Fragments arranged by size:
3396 1224 552 280 240
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2141 487
Size: 683 56 915 4038
Fragments arranged by size:
4038 915 683 56
BtsI GCAGTG_nn'
Cuts at: 2190 2969 3208 4002 4022 2190
Size: 779 239 794 20 3860
Fragments arranged by size:
3860 794 779 239 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5692
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5657
DraI TTT'AAA
Cuts at: 2996 2996
Size: 5692
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4699 809
Size: 507 3383 1802
Fragments arranged by size:
3383 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2103 2193 2298
Size: 54 1425 50 275 18 273 90 105
Cuts at: 2298 2403 2415 2505 2572 2667 3139 4028 5657
Size: 105 12 90 67 95 472 889 1629
Cuts at: 5657 8
Size: 43
Fragments arranged by size:
1629 1425 889 472 275 273 105 105
95 90 90 67 54 50 43 18
12
EagI C'GGCC_G
Cuts at: 1537 1812 2103 2298 2572 2667 3139 1537
Size: 275 291 195 274 95 472 4090
Fragments arranged by size:
4090 472 291 275 274 195 95
EarI CTCTTCn'nnn_
Cuts at: 1353 3622 1353
Size: 2269 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2483 4279 4653 4799 387
Size: 1064 138 894 1796 374 146 1280
Fragments arranged by size:
1796 1280 1064 894 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2351 2873 2885 3755 4352 5139 5407
Size: 393 719 522 12 870 597 787 268
Cuts at: 5407 1239
Size: 1524
Fragments arranged by size:
1524 870 787 719 597 522 393 268
12
EcoO109I rG'GnC_Cy
Cuts at: 2116 2259 2260 2356 2116
Size: 143 1 96 5452
Fragments arranged by size:
5452 143 96 1
EcoRI G'AATT_C
Cuts at: 3001 3379 3001
Size: 378 5314
FseI GG_CCGG'CC
Cuts at: 2304 2304
Size: 5692
FspI TGC'GCA
Cuts at: 4198 4198
Size: 5692
HaeII r_GCGC'y
Cuts at: 1678 2045 2066 2081 2221 2313 2596 2745 2756
Size: 367 21 15 140 92 283 149 11
Cuts at: 2756 4839 1678
Size: 2083 2531
Fragments arranged by size:
2531 2083 367 283 149 140 92 21
15 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2462 2494 2717 2749 4333
Size: 32 135 32 890 32 223 32 1584
Cuts at: 4333 4365 4407 4439 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1584 890 223 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3036 3282 669
Size: 988 281 1098 246 3079
Fragments arranged by size:
3079 1098 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5692
HpaI GTT'AAC
Cuts at: 3282 3282
Size: 5692
KasI G'GCGC_C
Cuts at: 2309 2741 2752 2309
Size: 432 11 5249
Fragments arranged by size:
5249 432 11
KpnI G_GTAC'C
Cuts at: 3017 3017
Size: 5692
MfeI C'AATT_G
Cuts at: 3291 3291
Size: 5692
MluI A'CGCG_T
Cuts at: 5568 5568
Size: 5692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4806 4990 1305
Size: 3501 184 2007
Fragments arranged by size:
3501 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2195 2417 10
Size: 54 1425 343 363 222 3285
Fragments arranged by size:
3285 1425 363 343 222 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3650 4009 4168 518
Size: 648 2484 359 159 2042
Fragments arranged by size:
2484 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2571 2770 2811 2920 3108 3785 4933 5178
Size: 965 199 41 109 188 677 1148 245
Cuts at: 5178 1606
Size: 2120
Fragments arranged by size:
2120 1148 965 677 245 199 188 109
41
NaeI GCC'GGC
Cuts at: 2302 2302
Size: 5692
NarI GG'CG_CC
Cuts at: 2310 2742 2753 2310
Size: 432 11 5249
Fragments arranged by size:
5249 432 11
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5140
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5692
NgoMIV G'CCGG_C
Cuts at: 2300 2300
Size: 5692
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5692
NotI GC'GGCC_GC
Cuts at: 2572 3139 2572
Size: 567 5125
NspI r_CATG'y
Cuts at: 1132 1229 2863 3050 4595 1132
Size: 97 1634 187 1545 2229
Fragments arranged by size:
2229 1634 1545 187 97
PasI CC'CwG_GG
Cuts at: 1348 2121 1348
Size: 773 4919
PciI A'CATG_T
Cuts at: 4591 4591
Size: 5692
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2830 1108
Size: 1722 3970
PmeI GTTT'AAAC
Cuts at: 2996 2996
Size: 5692
PmlI CAC'GTG
Cuts at: 2241 2443 2241
Size: 202 5490
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3761 3793 5274 5306 3761
Size: 32 1481 32 4147
Fragments arranged by size:
4147 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2116 2356 2116
Size: 240 5452
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5692
PsiI TTA'TAA
Cuts at: 3262 3262
Size: 5692
PspOMI G'GGCC_C
Cuts at: 2259 2259
Size: 5692
PstI C_TGCA'G
Cuts at: 830 2819 3044 830
Size: 1989 225 3478
Fragments arranged by size:
3478 1989 225
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5692
PvuII CAG'CTG
Cuts at: 2920 2920
Size: 5692
SacII CC_GC'GG
Cuts at: 2572 2812 2572
Size: 240 5452
SalI G'TCGA_C
Cuts at: 1936 3034 1936
Size: 1098 4594
SanDI GG'GwC_CC
Cuts at: 2116 2116
Size: 5692
SbfI CC_TGCA'GG
Cuts at: 3044 3044
Size: 5692
ScaI AGT'ACT
Cuts at: 3940 5652 3940
Size: 1712 3980
SexAI A'CCwGG_T
Cuts at: 2673 2829 2673
Size: 156 5536
SfcI C'TryA_G
Cuts at: 826 1045 2815 3040 4175 4856 5047 826
Size: 219 1770 225 1135 681 191 1471
Fragments arranged by size:
1770 1471 1135 681 225 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2423 2423
Size: 5692
SfoI GGC'GCC
Cuts at: 2311 2743 2754 2311
Size: 432 11 5249
Fragments arranged by size:
5249 432 11
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
SgrAI Cr'CCGG_yG
Cuts at: 1700 2755 1700
Size: 1055 4637
SmaI CCC'GGG
Cuts at: 1872 2113 2265 3019 1872
Size: 241 152 754 4545
Fragments arranged by size:
4545 754 241 152
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2462 3796 4697 4959 820
Size: 197 926 519 1334 901 262 1553
Fragments arranged by size:
1553 1334 926 901 519 262 197
SnaBI TAC'GTA
Cuts at: 493 3483 493
Size: 2990 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5692
SphI G_CATG'C
Cuts at: 1132 3050 1132
Size: 1918 3774
SspI AAT'ATT
Cuts at: 5 52 3616 5
Size: 47 3564 2081
Fragments arranged by size:
3564 2081 47
StuI AGG'CCT
Cuts at: 1517 2049 1517
Size: 532 5160
StyI C'CwwG_G
Cuts at: 513 1065 2406 3131 513
Size: 552 1341 725 3074
Fragments arranged by size:
3074 1341 725 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3719 3736 3889 4074 4519 325
Size: 1255 2139 17 153 185 445 1498
Fragments arranged by size:
2139 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3938 5650 96
Size: 274 80 33 51 1443 1961 1712 138
Fragments arranged by size:
1961 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5652 5684 5652
Size: 32 5660
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5692
XbaI T'CTAG_A
Cuts at: 3028 3028
Size: 5692
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5692
XmaI C'CCGG_G
Cuts at: 1870 2111 2263 3017 1870
Size: 241 152 754 4545
Fragments arranged by size:
4545 754 241 152
XmnI GAAnn'nnTTC
Cuts at: 3821 3821
Size: 5692
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2764 3500 276
Size: 53 83 186 936 1230 736 2468
Fragments arranged by size:
2468 1230 936 736 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacII SalI SanDI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FspAI NruI NsiI PacI PfoI PspXI PsrI RsrII
SacI SapI SrfI SwaI XcmI