Restriction map for
pFN21AE6691
(Circular) (Six-base) MAPSORT of: pFN21AE6691.seq Check: 1240 from: 1 to: 5659
pFN21AE6691 5659 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3469 278
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2211 2883 3002 1937
Size: 274 672 119 4594
Fragments arranged by size:
4594 672 274 119
Acc65I G'GTAC_C
Cuts at: 2980 2980
Size: 5659
AclI AA'CG_TT
Cuts at: 1563 3786 4159 1563
Size: 2223 373 3063
Fragments arranged by size:
3063 2223 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2287 2679 3722 5106 5374 1632
Size: 655 392 1043 1384 268 1917
Fragments arranged by size:
1917 1384 1043 655 392 268
AflII C'TTAA_G
Cuts at: 820 1017 2569 820
Size: 197 1552 3910
Fragments arranged by size:
3910 1552 197
AflIII A'CryG_T
Cuts at: 1203 2355 4558 5535 1203
Size: 1152 2203 977 1327
Fragments arranged by size:
2203 1327 1152 977
AgeI A'CCGG_T
Cuts at: 4490 4490
Size: 5659
AhdI GACnn_n'nnGTC
Cuts at: 4388 4388
Size: 5659
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5659
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5659
AlwNI CAG_nnn'CTG
Cuts at: 3402 4974 3402
Size: 1572 4087
ApaLI G'TGCA_C
Cuts at: 3716 4872 3716
Size: 1156 4503
ApoI r'AATT_y
Cuts at: 1493 2166 2968 3194 3346 5455 1493
Size: 673 802 226 152 2109 1697
Fragments arranged by size:
2109 1697 802 673 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2337 4213 160
Size: 872 1305 1876 1606
Fragments arranged by size:
1876 1606 1305 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
AvaI C'yCGr_G
Cuts at: 1870 1943 2984 1870
Size: 73 1041 4545
Fragments arranged by size:
4545 1041 73
BamHI G'GATC_C
Cuts at: 2488 2989 3356 2488
Size: 501 367 4791
Fragments arranged by size:
4791 501 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2980 4435 618
Size: 325 217 627 1193 1455 1842
Fragments arranged by size:
1842 1455 1193 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2193 2978 1391
Size: 802 785 4072
Fragments arranged by size:
4072 802 785
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4688
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3850 3884 1903
Size: 34 50 34 1829 34 3678
Fragments arranged by size:
3678 1829 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3551 4761 3551
Size: 1210 4449
BclI T'GATC_A
Cuts at: 1548 2067 1548
Size: 519 5140
BfrBI ATG'CAT
Cuts at: 2863 2921 2863
Size: 58 5601
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4270 136
Size: 107 122 71 631 605 2598 1525
Fragments arranged by size:
2598 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5654 5654
Size: 5659
BlpI GC'TnA_GC
Cuts at: 3076 3076
Size: 5659
Bme1580I G_kGCm'C
Cuts at: 1790 3720 4876 1790
Size: 1930 1156 2573
Fragments arranged by size:
2573 1930 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5659
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3390 4348 442
Size: 416 53 83 399 24 1973 958 1753
Fragments arranged by size:
1973 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2675 1056
Size: 1619 4040
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2550 2715 4319 1239
Size: 1311 165 1604 2579
Fragments arranged by size:
2579 1604 1311 165
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3784 4649 4947 3784
Size: 865 298 4496
Fragments arranged by size:
4496 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2400 2526 4322 882
Size: 617 901 126 1796 2219
Fragments arranged by size:
2219 1796 901 617 126
BsaAI yAC'GTr
Cuts at: 493 1204 3450 493
Size: 711 2246 2702
Fragments arranged by size:
2702 2246 711
BsaBI GATnn'nnATC
Cuts at: 5653 5653
Size: 5659
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3466 3848 4483 275
Size: 53 83 186 936 1933 382 635 1451
Fragments arranged by size:
1933 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4092 4490 4764 4911 5322 4092
Size: 398 274 147 411 4429
Fragments arranged by size:
4429 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2550 2580 1178
Size: 30 165 30 1147 30 4257
Fragments arranged by size:
4257 1147 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5659
BseYI C'CCAG_C
Cuts at: 4862 5358 4862
Size: 496 5163
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5086
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3109 3870 4898 1540
Size: 275 186 1108 761 1028 2301
Fragments arranged by size:
2301 1108 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2193 3720 3805 4876 2193
Size: 1527 85 1071 2976
Fragments arranged by size:
2976 1527 1071 85
BsmI GAATG_Cn'
Cuts at: 2115 3170 3263 2115
Size: 1055 93 4511
Fragments arranged by size:
4511 1055 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2193 2978 3720 3805 4876 1391
Size: 399 403 785 742 85 1071 2174
Fragments arranged by size:
2174 1071 785 742 403 399 85
BspEI T'CCGG_A
Cuts at: 5322 5322
Size: 5659
BspHI T'CATG_A
Cuts at: 3548 5278 3548
Size: 1730 3929
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3014 844
Size: 2170 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3546 1394
Size: 429 1723 3507
Fragments arranged by size:
3507 1723 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2784 4154 4328 65
Size: 1183 1536 1370 174 1396
Fragments arranged by size:
1536 1396 1370 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4303 4490 1700
Size: 110 2493 187 2869
Fragments arranged by size:
2869 2493 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5659
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5659
BssSI C'ACGA_G
Cuts at: 3719 4731 3719
Size: 1012 4647
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2711 2720 794
Size: 1917 9 3733
Fragments arranged by size:
3733 1917 9
BstBI TT'CG_AA
Cuts at: 4463 4463
Size: 5659
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5659
BstYI r'GATC_y
Cuts at: 1972 2488 2989 3356 3741 3758 5210 5296 5308
Size: 516 501 367 385 17 1452 86 12
Cuts at: 5308 5654 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 516 501 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2212 2884 2212
Size: 672 4987
Bsu36I CC'TnA_GG
Cuts at: 2891 2891
Size: 5659
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4920
Fragments arranged by size:
4920 683 56
BtsI GCAGTG_nn'
Cuts at: 3175 3969 3989 3175
Size: 794 20 4845
Fragments arranged by size:
4845 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5659
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2428 2463 529
Size: 35 1864 35 3725
Fragments arranged by size:
3725 1864 35 35
DraI TTT'AAA
Cuts at: 2963 2963
Size: 5659
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4666 809
Size: 507 3350 1802
Fragments arranged by size:
3350 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3106 3995 5624
Size: 54 1425 50 275 18 1276 889 1629
Cuts at: 5624 8
Size: 43
Fragments arranged by size:
1629 1425 1276 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3106 1537
Size: 275 1294 4090
Fragments arranged by size:
4090 1294 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3589 1353
Size: 2236 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4246 4620 4766 387
Size: 1064 138 2657 374 146 1280
Fragments arranged by size:
2657 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2191 2191
Size: 5659
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2287 2550 2679 2715 3722 4319 5106
Size: 393 655 263 129 36 1007 597 787
Cuts at: 5106 5374 1239
Size: 268 1524
Fragments arranged by size:
1524 1007 787 655 597 393 268 263
129 36
EcoRI G'AATT_C
Cuts at: 2968 3346 2968
Size: 378 5281
EcoRV GAT'ATC
Cuts at: 2158 2655 2158
Size: 497 5162
FspI TGC'GCA
Cuts at: 4165 4165
Size: 5659
HaeII r_GCGC'y
Cuts at: 1678 4806 1678
Size: 3128 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2550 2582 2839 2871 4300
Size: 32 135 32 978 32 257 32 1429
Cuts at: 4300 4332 4374 4406 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1429 978 257 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3003 3249 669
Size: 988 281 1065 246 3079
Fragments arranged by size:
3079 1065 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2376 748
Size: 1628 4031
HpaI GTT'AAC
Cuts at: 3249 3249
Size: 5659
KpnI G_GTAC'C
Cuts at: 2984 2984
Size: 5659
MfeI C'AATT_G
Cuts at: 3258 3258
Size: 5659
MluI A'CGCG_T
Cuts at: 5535 5535
Size: 5659
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4773 4957 1305
Size: 3468 184 2007
Fragments arranged by size:
3468 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3837
Fragments arranged by size:
3837 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2118 3617 3976 4135 518
Size: 648 952 1499 359 159 2042
Fragments arranged by size:
2042 1499 952 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3075 3752 4900 5145 1606
Size: 1469 677 1148 245 2120
Fragments arranged by size:
2120 1469 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5107
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5659
NheI G'CTAG_C
Cuts at: 1052 2671 1052
Size: 1619 4040
NotI GC'GGCC_GC
Cuts at: 3106 3106
Size: 5659
NsiI A_TGCA'T
Cuts at: 2865 2923 2865
Size: 58 5601
NspI r_CATG'y
Cuts at: 1132 1229 2359 2867 3017 4562 1132
Size: 97 1130 508 150 1545 2229
Fragments arranged by size:
2229 1545 1130 508 150 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5659
PciI A'CATG_T
Cuts at: 2355 4558 2355
Size: 2203 3456
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5659
PmeI GTTT'AAAC
Cuts at: 2963 2963
Size: 5659
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3728 3760 5241 5273 3728
Size: 32 1481 32 4114
Fragments arranged by size:
4114 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5659
PsiI TTA'TAA
Cuts at: 3229 3229
Size: 5659
PstI C_TGCA'G
Cuts at: 830 2260 3011 830
Size: 1430 751 3478
Fragments arranged by size:
3478 1430 751
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5659
SacI G_AGCT'C
Cuts at: 2193 2193
Size: 5659
SalI G'TCGA_C
Cuts at: 1936 3001 1936
Size: 1065 4594
SbfI CC_TGCA'GG
Cuts at: 3011 3011
Size: 5659
ScaI AGT'ACT
Cuts at: 3907 5619 3907
Size: 1712 3947
SexAI A'CCwGG_T
Cuts at: 2796 2796
Size: 5659
SfcI C'TryA_G
Cuts at: 826 1045 2256 3007 4142 4823 5014 826
Size: 219 1211 751 1135 681 191 1471
Fragments arranged by size:
1471 1211 1135 751 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5659
SmaI CCC'GGG
Cuts at: 1872 2986 1872
Size: 1114 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2569 3763 4664 4926 820
Size: 197 926 626 1194 901 262 1553
Fragments arranged by size:
1553 1194 926 901 626 262 197
SnaBI TAC'GTA
Cuts at: 493 3450 493
Size: 2957 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5659
SphI G_CATG'C
Cuts at: 1132 3017 1132
Size: 1885 3774
SspI AAT'ATT
Cuts at: 5 52 3583 5
Size: 47 3531 2081
Fragments arranged by size:
3531 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5659
StyI C'CwwG_G
Cuts at: 513 1065 3098 513
Size: 552 2033 3074
Fragments arranged by size:
3074 2033 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3686 3703 3856 4041 4486 325
Size: 1255 2106 17 153 185 445 1498
Fragments arranged by size:
2106 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3905 5617 96
Size: 274 80 33 51 1443 1928 1712 138
Fragments arranged by size:
1928 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5619 5651 5619
Size: 32 5627
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5659
XbaI T'CTAG_A
Cuts at: 2995 2995
Size: 5659
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5659
XmaI C'CCGG_G
Cuts at: 1870 2984 1870
Size: 1114 4545
XmnI GAAnn'nnTTC
Cuts at: 3788 3788
Size: 5659
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3467 276
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI DraIII EcoNI
EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI
SwaI XcmI