Restriction map for
pFN21AE6684
(Circular) (Six-base) MAPSORT of: pFN21AE6684.seq Check: 3107 from: 1 to: 5725
pFN21AE6684 5725 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3535 278
Size: 53 83 186 936 1999 2468
Fragments arranged by size:
2468 1999 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3068 1937
Size: 1131 4594
Acc65I G'GTAC_C
Cuts at: 3046 3046
Size: 5725
AclI AA'CG_TT
Cuts at: 1563 2343 3852 4225 1563
Size: 780 1509 373 3063
Fragments arranged by size:
3063 1509 780 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2099 3788 5172 5440 1632
Size: 467 1689 1384 268 1917
Fragments arranged by size:
1917 1689 1384 467 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5528
AflIII A'CryG_T
Cuts at: 1203 4624 5601 1203
Size: 3421 977 1327
Fragments arranged by size:
3421 1327 977
AgeI A'CCGG_T
Cuts at: 4556 4556
Size: 5725
AhdI GACnn_n'nnGTC
Cuts at: 4454 4454
Size: 5725
AleI CACnn'nnGTG
Cuts at: 1166 2151 2924 1166
Size: 985 773 3967
Fragments arranged by size:
3967 985 773
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5725
AlwNI CAG_nnn'CTG
Cuts at: 2128 2999 3468 5040 2128
Size: 871 469 1572 2813
Fragments arranged by size:
2813 1572 871 469
ApaLI G'TGCA_C
Cuts at: 3782 4938 3782
Size: 1156 4569
ApoI r'AATT_y
Cuts at: 1493 3034 3260 3412 5521 1493
Size: 1541 226 152 2109 1697
Fragments arranged by size:
2109 1697 1541 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4279 160
Size: 872 3247 1606
Fragments arranged by size:
3247 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
AvaI C'yCGr_G
Cuts at: 1870 1943 3050 1870
Size: 73 1107 4545
Fragments arranged by size:
4545 1107 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2560 2593 2560
Size: 33 5692
BamHI G'GATC_C
Cuts at: 3055 3422 3055
Size: 367 5358
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3046 4501 618
Size: 325 217 627 1259 1455 1842
Fragments arranged by size:
1842 1455 1259 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2019 3044 1391
Size: 628 1025 4072
Fragments arranged by size:
4072 1025 628
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2388 2727 928
Size: 971 489 339 3926
Fragments arranged by size:
3926 971 489 339
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2546 2580 3916 3950 1903
Size: 34 50 34 525 34 1336 34 3678
Fragments arranged by size:
3678 1336 525 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2824 3617 4827 2824
Size: 793 1210 3722
Fragments arranged by size:
3722 1210 793
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5725
BfrBI ATG'CAT
Cuts at: 2459 2625 2459
Size: 166 5559
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4336 136
Size: 107 122 71 631 605 2664 1525
Fragments arranged by size:
2664 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5720 5720
Size: 5725
BlpI GC'TnA_GC
Cuts at: 3142 3142
Size: 5725
Bme1580I G_kGCm'C
Cuts at: 1790 3786 4942 1790
Size: 1996 1156 2573
Fragments arranged by size:
2573 1996 1156
BmgBI CAC'GTC
Cuts at: 1318 2153 1318
Size: 835 4890
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3009 3456 4414
Size: 416 53 83 399 24 1592 447 958
Cuts at: 4414 442
Size: 1753
Fragments arranged by size:
1753 1592 958 447 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5725
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2003 4385 1239
Size: 764 2382 2579
Fragments arranged by size:
2579 2382 764
Bpu10I CC'TnA_GC
Cuts at: 2141 2141
Size: 5725
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3850 4715 5013 3850
Size: 865 298 4562
Fragments arranged by size:
4562 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2165 2209 4388 882
Size: 617 666 44 2179 2219
Fragments arranged by size:
2219 2179 666 617 44
BsaAI yAC'GTr
Cuts at: 493 1204 3516 493
Size: 711 2312 2702
Fragments arranged by size:
2702 2312 711
BsaBI GATnn'nnATC
Cuts at: 5719 5719
Size: 5725
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3532 3914 4549 275
Size: 53 83 186 936 1999 382 635 1451
Fragments arranged by size:
1999 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4158 4556 4830 4977 5388 4158
Size: 398 274 147 411 4495
Fragments arranged by size:
4495 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2777 2807 1178
Size: 30 165 30 1374 30 4096
Fragments arranged by size:
4096 1374 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2552 1186
Size: 1366 4359
BseYI C'CCAG_C
Cuts at: 2311 3007 4928 5424 2311
Size: 696 1921 496 2612
Fragments arranged by size:
2612 1921 696 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5152
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3175 3936 4964 1540
Size: 275 186 1174 761 1028 2301
Fragments arranged by size:
2301 1174 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2019 3786 3871 4942 2019
Size: 1767 85 1071 2802
Fragments arranged by size:
2802 1767 1071 85
BsmI GAATG_Cn'
Cuts at: 2627 3236 3329 2627
Size: 609 93 5023
Fragments arranged by size:
5023 609 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2019 3044 3786 3871 4942 1391
Size: 399 229 1025 742 85 1071 2174
Fragments arranged by size:
2174 1071 1025 742 399 229 85
BspEI T'CCGG_A
Cuts at: 5388 5388
Size: 5725
BspHI T'CATG_A
Cuts at: 3614 5344 3614
Size: 1730 3995
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3080 844
Size: 2236 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3612 1394
Size: 429 1789 3507
Fragments arranged by size:
3507 1789 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4220 4394 65
Size: 1183 2972 174 1396
Fragments arranged by size:
2972 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4369 4556 1700
Size: 110 2559 187 2869
Fragments arranged by size:
2869 2559 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5725
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5725
BssSI C'ACGA_G
Cuts at: 3785 4797 3785
Size: 1012 4713
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5725
BstBI TT'CG_AA
Cuts at: 4529 4529
Size: 5725
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5725
BstXI CCAn_nnnn'nTGG
Cuts at: 2153 2153
Size: 5725
BstYI r'GATC_y
Cuts at: 1972 3055 3422 3807 3824 5276 5362 5374 5720
Size: 1083 367 385 17 1452 86 12 346
Cuts at: 5720 1972
Size: 1977
Fragments arranged by size:
1977 1452 1083 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2535 2744 2535
Size: 209 5516
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5173
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4986
Fragments arranged by size:
4986 683 56
BtsI GCAGTG_nn'
Cuts at: 3241 4035 4055 3241
Size: 794 20 4911
Fragments arranged by size:
4911 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5725
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2181 2216 529
Size: 35 1617 35 4038
Fragments arranged by size:
4038 1617 35 35
DraI TTT'AAA
Cuts at: 3029 3029
Size: 5725
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2510 4732 809
Size: 507 1194 2222 1802
Fragments arranged by size:
2222 1802 1194 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2469 3172 4061
Size: 54 1425 50 275 18 639 703 889
Cuts at: 4061 5690 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 703 639 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3172 1537
Size: 275 1360 4090
Fragments arranged by size:
4090 1360 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2099 2357 3655 1353
Size: 746 258 1298 3423
Fragments arranged by size:
3423 1298 746 258
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4312 4686 4832 387
Size: 1064 138 2723 374 146 1280
Fragments arranged by size:
2723 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2017 2017
Size: 5725
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2003 2099 3788 4385 5172 5440 1239
Size: 393 371 96 1689 597 787 268 1524
Fragments arranged by size:
1689 1524 787 597 393 371 268 96
EcoO109I rG'GnC_Cy
Cuts at: 2675 2968 2675
Size: 293 5432
EcoRI G'AATT_C
Cuts at: 3034 3412 3034
Size: 378 5347
FspI TGC'GCA
Cuts at: 2556 4231 2556
Size: 1675 4050
HaeII r_GCGC'y
Cuts at: 1678 2815 4872 1678
Size: 1137 2057 2531
Fragments arranged by size:
2531 2057 1137
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2501 2533 4366 4398 4440
Size: 32 135 32 929 32 1833 32 42
Cuts at: 4440 4472 1373
Size: 32 2626
Fragments arranged by size:
2626 1833 929 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3069 3315 669
Size: 988 281 1131 246 3079
Fragments arranged by size:
3079 1131 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2842 748
Size: 2094 3631
HpaI GTT'AAC
Cuts at: 3315 3315
Size: 5725
KpnI G_GTAC'C
Cuts at: 3050 3050
Size: 5725
MfeI C'AATT_G
Cuts at: 3324 3324
Size: 5725
MluI A'CGCG_T
Cuts at: 5601 5601
Size: 5725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4839 5023 1305
Size: 3534 184 2007
Fragments arranged by size:
3534 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2471 10
Size: 54 1425 343 639 3264
Fragments arranged by size:
3264 1425 639 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2151 2288 2498 2924 3683 4042 4201
Size: 648 985 137 210 426 759 359 159
Cuts at: 4201 518
Size: 2042
Fragments arranged by size:
2042 985 759 648 426 359 210 159
137
MspA1I CmG'CkG
Cuts at: 1606 2088 3141 3818 4966 5211 1606
Size: 482 1053 677 1148 245 2120
Fragments arranged by size:
2120 1148 1053 677 482 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5173
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5725
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5725
NotI GC'GGCC_GC
Cuts at: 3172 3172
Size: 5725
NsiI A_TGCA'T
Cuts at: 2461 2627 2461
Size: 166 5559
NspI r_CATG'y
Cuts at: 1132 1229 3083 4628 1132
Size: 97 1854 1545 2229
Fragments arranged by size:
2229 1854 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5725
PciI A'CATG_T
Cuts at: 4624 4624
Size: 5725
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2128 2478 1108
Size: 1020 350 4355
Fragments arranged by size:
4355 1020 350
PfoI T'CCnGG_A
Cuts at: 2727 2727
Size: 5725
PmeI GTTT'AAAC
Cuts at: 3029 3029
Size: 5725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3794 3826 5307 5339 3794
Size: 32 1481 32 4180
Fragments arranged by size:
4180 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2968 2968
Size: 5725
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5725
PsiI TTA'TAA
Cuts at: 3295 3295
Size: 5725
PstI C_TGCA'G
Cuts at: 830 3077 830
Size: 2247 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5725
PvuII CAG'CTG
Cuts at: 2088 2088
Size: 5725
SacI G_AGCT'C
Cuts at: 2019 2019
Size: 5725
SalI G'TCGA_C
Cuts at: 1936 3067 1936
Size: 1131 4594
SbfI CC_TGCA'GG
Cuts at: 3077 3077
Size: 5725
ScaI AGT'ACT
Cuts at: 3973 5685 3973
Size: 1712 4013
SfcI C'TryA_G
Cuts at: 826 1045 3073 4208 4889 5080 826
Size: 219 2028 1135 681 191 1471
Fragments arranged by size:
2028 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5725
SmaI CCC'GGG
Cuts at: 1872 3052 1872
Size: 1180 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3829 4730 4992 820
Size: 197 926 1886 901 262 1553
Fragments arranged by size:
1886 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3516 493
Size: 3023 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5725
SphI G_CATG'C
Cuts at: 1132 3083 1132
Size: 1951 3774
SspI AAT'ATT
Cuts at: 5 52 2586 3649 5
Size: 47 2534 1063 2081
Fragments arranged by size:
2534 2081 1063 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5725
StyI C'CwwG_G
Cuts at: 513 1065 2671 3164 513
Size: 552 1606 493 3074
Fragments arranged by size:
3074 1606 552 493
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3752 3769 3922 4107 4552 325
Size: 1255 2172 17 153 185 445 1498
Fragments arranged by size:
2172 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3971 5683 96
Size: 274 80 33 51 1443 1994 1712 138
Fragments arranged by size:
1994 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2473 2505 5685 5717 2473
Size: 32 3180 32 2481
Fragments arranged by size:
3180 2481 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5725
XbaI T'CTAG_A
Cuts at: 2167 3061 2167
Size: 894 4831
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2397 2397
Size: 5725
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5725
XmaI C'CCGG_G
Cuts at: 1870 3050 1870
Size: 1180 4545
XmnI GAAnn'nnTTC
Cuts at: 3854 3854
Size: 5725
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3533 276
Size: 53 83 186 936 1999 2468
Fragments arranged by size:
2468 1999 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI