Restriction map for
pFN21AE6680
(Circular) (Six-base) MAPSORT of: pFN21AE6680.seq Check: 3825 from: 1 to: 5719
pFN21AE6680 5719 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2037 2232 2922 3529
Size: 53 83 186 936 501 195 690 607
Cuts at: 3529 278
Size: 2468
Fragments arranged by size:
2468 936 690 607 501 195 186 83
53
AccI GT'mk_AC
Cuts at: 1937 2778 3062 1937
Size: 841 284 4594
Fragments arranged by size:
4594 841 284
Acc65I G'GTAC_C
Cuts at: 3040 3040
Size: 5719
AclI AA'CG_TT
Cuts at: 1563 3846 4219 1563
Size: 2283 373 3063
Fragments arranged by size:
3063 2283 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3011 3782 5166 5434 1632
Size: 1379 771 1384 268 1917
Fragments arranged by size:
1917 1384 1379 771 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5522
AflIII A'CryG_T
Cuts at: 1203 2256 2279 4618 5595 1203
Size: 1053 23 2339 977 1327
Fragments arranged by size:
2339 1327 1053 977 23
AgeI A'CCGG_T
Cuts at: 4550 4550
Size: 5719
AhdI GACnn_n'nnGTC
Cuts at: 4448 4448
Size: 5719
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5719
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5719
AlwNI CAG_nnn'CTG
Cuts at: 3462 5034 3462
Size: 1572 4147
ApaI G_GGCC'C
Cuts at: 2734 2734
Size: 5719
ApaLI G'TGCA_C
Cuts at: 3776 4932 3776
Size: 1156 4563
ApoI r'AATT_y
Cuts at: 1493 3028 3254 3406 5515 1493
Size: 1535 226 152 2109 1697
Fragments arranged by size:
2109 1697 1535 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4273 160
Size: 872 3241 1606
Fragments arranged by size:
3241 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
AvaI C'yCGr_G
Cuts at: 1870 1943 2981 3044 1870
Size: 73 1038 63 4545
Fragments arranged by size:
4545 1038 73 63
BamHI G'GATC_C
Cuts at: 2963 3049 3416 2963
Size: 86 367 5266
Fragments arranged by size:
5266 367 86
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2017 2947 3040 4495 618
Size: 325 217 627 230 930 93 1455 1842
Fragments arranged by size:
1842 1455 930 627 325 230 217 93
BanII G_rGCy'C
Cuts at: 1391 2734 2747 2819 3038 1391
Size: 1343 13 72 219 4072
Fragments arranged by size:
4072 1343 219 72 13
BbeI G_GCGC'C
Cuts at: 2021 2951 2021
Size: 930 4789
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4748
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3910 3944 1903
Size: 34 1973 34 3678
Fragments arranged by size:
3678 1973 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3611 4821 3611
Size: 1210 4509
BclI T'GATC_A
Cuts at: 1548 2209 1548
Size: 661 5058
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4330 136
Size: 107 122 71 631 605 2658 1525
Fragments arranged by size:
2658 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5714 5714
Size: 5719
BlpI GC'TnA_GC
Cuts at: 3136 3136
Size: 5719
Bme1580I G_kGCm'C
Cuts at: 1790 2734 3780 4936 1790
Size: 944 1046 1156 2573
Fragments arranged by size:
2573 1156 1046 944
BmgBI CAC'GTC
Cuts at: 1318 2977 1318
Size: 1659 4060
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2907 3450 4408
Size: 416 53 83 399 24 1490 543 958
Cuts at: 4408 442
Size: 1753
Fragments arranged by size:
1753 1490 958 543 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5719
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3025 3025
Size: 5719
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4379 1239
Size: 3140 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3844 4709 5007 3844
Size: 865 298 4556
Fragments arranged by size:
4556 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4382 882
Size: 617 2883 2219
Fragments arranged by size:
2883 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2280 3510 493
Size: 711 1076 1230 2702
Fragments arranged by size:
2702 1230 1076 711
BsaBI GATnn'nnATC
Cuts at: 2763 5713 2763
Size: 2950 2769
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2018 2034 2229 2919
Size: 53 83 186 936 485 16 195 690
Cuts at: 2919 2948 3526 3908 4543 275
Size: 29 578 382 635 1451
Fragments arranged by size:
1451 936 690 635 578 485 382 195
186 83 53 29 16
BsaWI w'CCGG_w
Cuts at: 2326 4152 4550 4824 4971 5382 2326
Size: 1826 398 274 147 411 2663
Fragments arranged by size:
2663 1826 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2219 2249 2897 2927 1178
Size: 30 165 30 816 30 648 30 3970
Fragments arranged by size:
3970 816 648 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 1186
Size: 892 4827
BseYI C'CCAG_C
Cuts at: 2100 2879 4922 5418 2100
Size: 779 2043 496 2401
Fragments arranged by size:
2401 2043 779 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2104 1163
Size: 573 368 4778
Fragments arranged by size:
4778 573 368
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3169 3930 4958 1540
Size: 275 186 1168 761 1028 2301
Fragments arranged by size:
2301 1168 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2982 3780 3865 4936 2982
Size: 798 85 1071 3765
Fragments arranged by size:
3765 1071 798 85
BsmI GAATG_Cn'
Cuts at: 2852 3230 3323 2852
Size: 378 93 5248
Fragments arranged by size:
5248 378 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2734 2747 2819 2982 3038 3780 3865
Size: 399 944 13 72 163 56 742 85
Cuts at: 3865 4936 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 944 742 399 163 85 72
56 13
BspEI T'CCGG_A
Cuts at: 5382 5382
Size: 5719
BspHI T'CATG_A
Cuts at: 3608 5338 3608
Size: 1730 3989
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3074 844
Size: 2230 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3606 1394
Size: 429 1783 3507
Fragments arranged by size:
3507 1783 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2181 4214 4388 65
Size: 1183 933 2033 174 1396
Fragments arranged by size:
2033 1396 1183 933 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2492 2950 4363 4550 1700
Size: 110 682 458 1413 187 2869
Fragments arranged by size:
2869 1413 682 458 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5719
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5719
BssSI C'ACGA_G
Cuts at: 2288 3779 4791 2288
Size: 1491 1012 3216
Fragments arranged by size:
3216 1491 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5719
BstBI TT'CG_AA
Cuts at: 4523 4523
Size: 5719
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5719
BstXI CCAn_nnnn'nTGG
Cuts at: 2245 2320 2524 2245
Size: 75 204 5440
Fragments arranged by size:
5440 204 75
BstYI r'GATC_y
Cuts at: 1972 2372 2963 3049 3416 3801 3818 5270 5356
Size: 400 591 86 367 385 17 1452 86
Cuts at: 5356 5368 5714 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 591 400 385 367 346 86
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2080 2535 2748 2856 513
Size: 552 939 76 455 213 108 3376
Fragments arranged by size:
3376 939 552 455 213 108 76
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2149 2750 487
Size: 683 56 923 601 3456
Fragments arranged by size:
3456 923 683 601 56
BtsI GCAGTG_nn'
Cuts at: 2518 3235 4029 4049 2518
Size: 717 794 20 4188
Fragments arranged by size:
4188 794 717 20
ClaI AT'CG_AT
Cuts at: 1554 2385 1554
Size: 831 4888
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5684
DraI TTT'AAA
Cuts at: 3023 3023
Size: 5719
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2033 2546 2672 4726 809
Size: 507 717 513 126 2054 1802
Fragments arranged by size:
2054 1802 717 513 507 126
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2571 2889 3166
Size: 54 1425 50 275 18 741 318 277
Cuts at: 3166 4055 5684 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 741 318 277 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3166 1537
Size: 275 1354 4090
Fragments arranged by size:
4090 1354 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2146 2807 3649 1353
Size: 793 661 842 3423
Fragments arranged by size:
3423 842 793 661
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4306 4680 4826 387
Size: 1064 138 2717 374 146 1280
Fragments arranged by size:
2717 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3011 3782 4379 5166 5434 1239
Size: 393 1379 771 597 787 268 1524
Fragments arranged by size:
1524 1379 787 771 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2642 2642
Size: 5719
EcoO109I rG'GnC_Cy
Cuts at: 2730 2730
Size: 5719
EcoRI G'AATT_C
Cuts at: 3028 3406 3028
Size: 378 5341
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2287 2287
Size: 5719
FspI TGC'GCA
Cuts at: 2153 4225 2153
Size: 2072 3647
HaeII r_GCGC'y
Cuts at: 1678 2021 2951 4866 1678
Size: 343 930 1915 2531
Fragments arranged by size:
2531 1915 930 343
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2194 2219 2226 2251 4360
Size: 32 135 32 622 25 7 25 2109
Cuts at: 4360 4392 4434 4466 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2109 622 135 42 32 32 32
32 25 25 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2038 2097 2551 3063 3309 669
Size: 988 281 100 59 454 512 246 3079
Fragments arranged by size:
3079 988 512 454 281 246 100 59
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5719
HpaI GTT'AAC
Cuts at: 3309 3309
Size: 5719
KasI G'GCGC_C
Cuts at: 2017 2947 2017
Size: 930 4789
KpnI G_GTAC'C
Cuts at: 3044 3044
Size: 5719
MfeI C'AATT_G
Cuts at: 3318 3318
Size: 5719
MluI A'CGCG_T
Cuts at: 5595 5595
Size: 5719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2262 2383 2403 4833 5017 1305
Size: 957 121 20 2430 184 2007
Fragments arranged by size:
2430 2007 957 184 121 20
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2891 10
Size: 54 1425 343 1059 2838
Fragments arranged by size:
2838 1425 1059 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2175 2318 2591 3677 4036 4195 518
Size: 648 1009 143 273 1086 359 159 2042
Fragments arranged by size:
2042 1086 1009 648 359 273 159 143
MspA1I CmG'CkG
Cuts at: 1606 2082 2104 3135 3812 4960 5205 1606
Size: 476 22 1031 677 1148 245 2120
Fragments arranged by size:
2120 1148 1031 677 476 245 22
NaeI GCC'GGC
Cuts at: 2494 2494
Size: 5719
NarI GG'CG_CC
Cuts at: 2018 2948 2018
Size: 930 4789
NcoI C'CATG_G
Cuts at: 513 1065 2004 2535 2748 2856 513
Size: 552 939 531 213 108 3376
Fragments arranged by size:
3376 939 552 531 213 108
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5719
NgoMIV G'CCGG_C
Cuts at: 2492 2492
Size: 5719
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5719
NotI GC'GGCC_GC
Cuts at: 3166 3166
Size: 5719
NspI r_CATG'y
Cuts at: 1132 1229 2260 3077 4622 1132
Size: 97 1031 817 1545 2229
Fragments arranged by size:
2229 1545 1031 817 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5719
PciI A'CATG_T
Cuts at: 2256 4618 2256
Size: 2362 3357
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5719
PmeI GTTT'AAAC
Cuts at: 3023 3023
Size: 5719
PmlI CAC'GTG
Cuts at: 2280 2280
Size: 5719
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3788 3820 5301 5333 3788
Size: 32 1481 32 4174
Fragments arranged by size:
4174 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5719
PsiI TTA'TAA
Cuts at: 3289 3289
Size: 5719
PspOMI G'GGCC_C
Cuts at: 2730 2730
Size: 5719
PspXI vC'TCGA_Gb
Cuts at: 2981 2981
Size: 5719
PstI C_TGCA'G
Cuts at: 830 3071 830
Size: 2241 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5719
PvuII CAG'CTG
Cuts at: 2104 2104
Size: 5719
SacII CC_GC'GG
Cuts at: 2083 2083
Size: 5719
SalI G'TCGA_C
Cuts at: 1936 3061 1936
Size: 1125 4594
SapI GCTCTTCn'nnn_
Cuts at: 2807 2807
Size: 5719
SbfI CC_TGCA'GG
Cuts at: 3071 3071
Size: 5719
ScaI AGT'ACT
Cuts at: 3967 5679 3967
Size: 1712 4007
SexAI A'CCwGG_T
Cuts at: 2244 2481 2244
Size: 237 5482
SfcI C'TryA_G
Cuts at: 826 1045 2422 2632 3067 4202 4883 5074 826
Size: 219 1377 210 435 1135 681 191 1471
Fragments arranged by size:
1471 1377 1135 681 435 219 210 191
SfoI GGC'GCC
Cuts at: 2019 2949 2019
Size: 930 4789
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5719
SgrAI Cr'CCGG_yG
Cuts at: 1700 2950 1700
Size: 1250 4469
SmaI CCC'GGG
Cuts at: 1872 3046 1872
Size: 1174 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2981 3823 4724 4986 820
Size: 197 926 1038 842 901 262 1553
Fragments arranged by size:
1553 1038 926 901 842 262 197
SnaBI TAC'GTA
Cuts at: 493 3510 493
Size: 3017 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5719
SphI G_CATG'C
Cuts at: 1132 3077 1132
Size: 1945 3774
SspI AAT'ATT
Cuts at: 5 52 3643 5
Size: 47 3591 2081
Fragments arranged by size:
3591 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5719
StyI C'CwwG_G
Cuts at: 513 1065 2004 2535 2748 2856 3158 513
Size: 552 939 531 213 108 302 3074
Fragments arranged by size:
3074 939 552 531 302 213 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3746 3763 3916 4101 4546 325
Size: 1255 2166 17 153 185 445 1498
Fragments arranged by size:
2166 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2682 3965 5677
Size: 274 80 33 51 1443 705 1283 1712
Cuts at: 5677 96
Size: 138
Fragments arranged by size:
1712 1443 1283 705 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5679 5711 5679
Size: 32 5687
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5719
XbaI T'CTAG_A
Cuts at: 3055 3055
Size: 5719
XhoI C'TCGA_G
Cuts at: 1943 2981 1943
Size: 1038 4681
XmaI C'CCGG_G
Cuts at: 1870 3044 1870
Size: 1174 4545
XmnI GAAnn'nnTTC
Cuts at: 2699 3848 2699
Size: 1149 4570
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2035 2230 2920 3527
Size: 53 83 186 936 501 195 690 607
Cuts at: 3527 276
Size: 2468
Fragments arranged by size:
2468 936 690 607 501 195 186 83
53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FseI FspAI NruI NsiI PacI PfoI PpuMI PsrI
RsrII SacI SanDI SfiI SrfI SwaI XcmI