Restriction map for
pFN21AE6676
(Circular) (Six-base) MAPSORT of: pFN21AE6676.seq Check: 5871 from: 1 to: 5563
pFN21AE6676 5563 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3373 278
Size: 53 83 186 936 1837 2468
Fragments arranged by size:
2468 1837 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2906 1937
Size: 969 4594
Acc65I G'GTAC_C
Cuts at: 2884 2884
Size: 5563
AclI AA'CG_TT
Cuts at: 1563 3690 4063 1563
Size: 2127 373 3063
Fragments arranged by size:
3063 2127 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3626 5010 5278 1632
Size: 1994 1384 268 1917
Fragments arranged by size:
1994 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5366
AflIII A'CryG_T
Cuts at: 1203 2210 2601 2634 4462 5439 1203
Size: 1007 391 33 1828 977 1327
Fragments arranged by size:
1828 1327 1007 977 391 33
AgeI A'CCGG_T
Cuts at: 4394 4394
Size: 5563
AhdI GACnn_n'nnGTC
Cuts at: 4292 4292
Size: 5563
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5563
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5563
AlwNI CAG_nnn'CTG
Cuts at: 2590 2845 3306 4878 2590
Size: 255 461 1572 3275
Fragments arranged by size:
3275 1572 461 255
ApaLI G'TGCA_C
Cuts at: 3620 4776 3620
Size: 1156 4407
ApoI r'AATT_y
Cuts at: 1493 2872 3098 3250 5359 1493
Size: 1379 226 152 2109 1697
Fragments arranged by size:
2109 1697 1379 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2739 4117 160
Size: 872 1707 1378 1606
Fragments arranged by size:
1707 1606 1378 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5563
AvaI C'yCGr_G
Cuts at: 1870 1943 2888 1870
Size: 73 945 4545
Fragments arranged by size:
4545 945 73
BamHI G'GATC_C
Cuts at: 2070 2893 3260 2070
Size: 823 367 4373
Fragments arranged by size:
4373 823 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2884 4339 618
Size: 325 217 627 1097 1455 1842
Fragments arranged by size:
1842 1455 1097 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2257 2882 1391
Size: 866 625 4072
Fragments arranged by size:
4072 866 625
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2095 2182 2768 928
Size: 971 196 87 586 3723
Fragments arranged by size:
3723 971 586 196 87
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3754 3788 1903
Size: 34 1817 34 3678
Fragments arranged by size:
3678 1817 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3455 4665 3455
Size: 1210 4353
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5563
BfrBI ATG'CAT
Cuts at: 2060 2060
Size: 5563
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2288 4174 136
Size: 107 122 71 631 605 616 1886 1525
Fragments arranged by size:
1886 1525 631 616 605 122 107 71
BglII A'GATC_T
Cuts at: 5558 5558
Size: 5563
BlpI GC'TnA_GC
Cuts at: 2980 2980
Size: 5563
Bme1580I G_kGCm'C
Cuts at: 1790 2030 3624 4780 1790
Size: 240 1594 1156 2573
Fragments arranged by size:
2573 1594 1156 240
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5563
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2668 3294 4252
Size: 416 53 83 399 24 1251 626 958
Cuts at: 4252 442
Size: 1753
Fragments arranged by size:
1753 1251 958 626 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5563
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2522 4223 1239
Size: 1283 1701 2579
Fragments arranged by size:
2579 1701 1283
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3688 4553 4851 3688
Size: 865 298 4400
Fragments arranged by size:
4400 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4226 882
Size: 617 2727 2219
Fragments arranged by size:
2727 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3354 493
Size: 711 2150 2702
Fragments arranged by size:
2702 2150 711
BsaBI GATnn'nnATC
Cuts at: 5557 5557
Size: 5563
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2115 3370 3752 4387
Size: 53 83 186 936 582 1255 382 635
Cuts at: 4387 275
Size: 1451
Fragments arranged by size:
1451 1255 936 635 582 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2388 2782 3996 4394 4668 4815 5226 2388
Size: 394 1214 398 274 147 411 2725
Fragments arranged by size:
2725 1214 411 398 394 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5338
Fragments arranged by size:
5338 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5563
BseYI C'CCAG_C
Cuts at: 2260 4766 5262 2260
Size: 2506 496 2561
Fragments arranged by size:
2561 2506 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2235 1163
Size: 573 499 4491
Fragments arranged by size:
4491 573 499
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3013 3774 4802 1540
Size: 275 186 1012 761 1028 2301
Fragments arranged by size:
2301 1028 1012 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2237 2513 3624 3709 4780 2237
Size: 276 1111 85 1071 3020
Fragments arranged by size:
3020 1111 1071 276 85
BsmI GAATG_Cn'
Cuts at: 2158 3074 3167 2158
Size: 916 93 4554
Fragments arranged by size:
4554 916 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2237 2257 2513 2882 3624 3709
Size: 399 240 207 20 256 369 742 85
Cuts at: 3709 4780 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 369 256 240 207
85 20
BspEI T'CCGG_A
Cuts at: 2388 5226 2388
Size: 2838 2725
BspHI T'CATG_A
Cuts at: 3452 5182 3452
Size: 1730 3833
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2531 2918 844
Size: 1687 387 3489
Fragments arranged by size:
3489 1687 387
BsrBI CCG'CTC
Cuts at: 1394 1823 2076 3450 1394
Size: 429 253 1374 3507
Fragments arranged by size:
3507 1374 429 253
BsrDI GCAATG_nn'
Cuts at: 65 1248 4058 4232 65
Size: 1183 2810 174 1396
Fragments arranged by size:
2810 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4207 4394 1700
Size: 110 2397 187 2869
Fragments arranged by size:
2869 2397 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5563
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5563
BssSI C'ACGA_G
Cuts at: 3623 4635 3623
Size: 1012 4551
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2845 794
Size: 2051 3512
BstBI TT'CG_AA
Cuts at: 4367 4367
Size: 5563
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5563
BstXI CCAn_nnnn'nTGG
Cuts at: 2086 2086
Size: 5563
BstYI r'GATC_y
Cuts at: 1972 2070 2893 3260 3645 3662 5114 5200 5212
Size: 98 823 367 385 17 1452 86 12
Cuts at: 5212 5558 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 823 385 367 346 98 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5011
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2034 487
Size: 683 56 808 4016
Fragments arranged by size:
4016 808 683 56
BtsI GCAGTG_nn'
Cuts at: 2608 3079 3873 3893 2608
Size: 471 794 20 4278
Fragments arranged by size:
4278 794 471 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5563
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2275 2310 529
Size: 35 1711 35 3782
Fragments arranged by size:
3782 1711 35 35
DraI TTT'AAA
Cuts at: 2867 2867
Size: 5563
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2669 4570 809
Size: 507 1353 1901 1802
Fragments arranged by size:
1901 1802 1353 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2175 2205 2296
Size: 54 1425 50 275 18 345 30 91
Cuts at: 2296 2470 3010 3899 5528 8
Size: 174 540 889 1629 43
Fragments arranged by size:
1629 1425 889 540 345 275 174 91
54 50 43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 3010 1537
Size: 275 1198 4090
Fragments arranged by size:
4090 1198 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2149 2849 3493 1353
Size: 796 700 644 3423
Fragments arranged by size:
3423 796 700 644
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4150 4524 4670 387
Size: 1064 138 2561 374 146 1280
Fragments arranged by size:
2561 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2522 3626 4223 5010 5278 1239
Size: 393 890 1104 597 787 268 1524
Fragments arranged by size:
1524 1104 890 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2197 2266 2197
Size: 69 5494
EcoRI G'AATT_C
Cuts at: 2872 3250 2872
Size: 378 5185
FspI TGC'GCA
Cuts at: 4069 4069
Size: 5563
HaeII r_GCGC'y
Cuts at: 1678 4710 1678
Size: 3032 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2045 2077 2172 2204 4204
Size: 32 135 32 473 32 95 32 2000
Cuts at: 4204 4236 4278 4310 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2000 473 135 95 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2907 3153 669
Size: 988 281 969 246 3079
Fragments arranged by size:
3079 988 969 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5563
HpaI GTT'AAC
Cuts at: 3153 3153
Size: 5563
KpnI G_GTAC'C
Cuts at: 2888 2888
Size: 5563
MfeI C'AATT_G
Cuts at: 3162 3162
Size: 5563
MluI A'CGCG_T
Cuts at: 2210 5439 2210
Size: 3229 2334
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2400 2692 4677 4861 1305
Size: 1095 292 1985 184 2007
Fragments arranged by size:
2007 1985 1095 292 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2177 2298 2472 10
Size: 54 1425 343 345 121 174 3101
Fragments arranged by size:
3101 1425 345 343 174 121 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2084 3521 3880 4039 518
Size: 648 918 1437 359 159 2042
Fragments arranged by size:
2042 1437 918 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2137 2842 2979 3656 4804 5049 1606
Size: 531 705 137 677 1148 245 2120
Fragments arranged by size:
2120 1148 705 677 531 245 137
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5011
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5563
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5563
NotI GC'GGCC_GC
Cuts at: 3010 3010
Size: 5563
NsiI A_TGCA'T
Cuts at: 2062 2062
Size: 5563
NspI r_CATG'y
Cuts at: 1132 1229 2282 2605 2638 2921 4466 1132
Size: 97 1053 323 33 283 1545 2229
Fragments arranged by size:
2229 1545 1053 323 283 97 33
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5563
PciI A'CATG_T
Cuts at: 2601 2634 4462 2601
Size: 33 1828 3702
Fragments arranged by size:
3702 1828 33
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5563
PfoI T'CCnGG_A
Cuts at: 2499 2531 2499
Size: 32 5531
PmeI GTTT'AAAC
Cuts at: 2867 2867
Size: 5563
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3632 3664 5145 5177 3632
Size: 32 1481 32 4018
Fragments arranged by size:
4018 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2197 2266 2197
Size: 69 5494
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5563
PsiI TTA'TAA
Cuts at: 3133 3133
Size: 5563
PstI C_TGCA'G
Cuts at: 830 2915 830
Size: 2085 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5563
PvuII CAG'CTG
Cuts at: 2137 2842 2137
Size: 705 4858
SalI G'TCGA_C
Cuts at: 1936 2905 1936
Size: 969 4594
SanDI GG'GwC_CC
Cuts at: 2266 2266
Size: 5563
SbfI CC_TGCA'GG
Cuts at: 2915 2915
Size: 5563
ScaI AGT'ACT
Cuts at: 3811 5523 3811
Size: 1712 3851
SfcI C'TryA_G
Cuts at: 826 1045 2911 4046 4727 4918 826
Size: 219 1866 1135 681 191 1471
Fragments arranged by size:
1866 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5563
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5563
SmaI CCC'GGG
Cuts at: 1872 2890 1872
Size: 1018 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3667 4568 4830 820
Size: 197 926 1724 901 262 1553
Fragments arranged by size:
1724 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3354 493
Size: 2861 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5563
SphI G_CATG'C
Cuts at: 1132 2282 2921 1132
Size: 1150 639 3774
Fragments arranged by size:
3774 1150 639
SspI AAT'ATT
Cuts at: 5 52 3487 5
Size: 47 3435 2081
Fragments arranged by size:
3435 2081 47
StuI AGG'CCT
Cuts at: 1517 2247 1517
Size: 730 4833
StyI C'CwwG_G
Cuts at: 513 1065 3002 513
Size: 552 1937 3074
Fragments arranged by size:
3074 1937 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2632 3590 3607 3760 3945 4390 325
Size: 1255 1052 958 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1052 958 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3809 5521 96
Size: 274 80 33 51 1443 1832 1712 138
Fragments arranged by size:
1832 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2123 2155 5523 5555 2123
Size: 32 3368 32 2131
Fragments arranged by size:
3368 2131 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5563
XbaI T'CTAG_A
Cuts at: 2899 2899
Size: 5563
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2497 2497
Size: 5563
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5563
XmaI C'CCGG_G
Cuts at: 1870 2888 1870
Size: 1018 4545
XmnI GAAnn'nnTTC
Cuts at: 2189 3692 2189
Size: 1503 4060
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3371 276
Size: 53 83 186 936 1837 2468
Fragments arranged by size:
2468 1837 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SapI SexAI SfiI SfoI SrfI
SwaI