Restriction map for
pFN21AE6673
(Circular) (Six-base) MAPSORT of: pFN21AE6673.seq Check: 81 from: 1 to: 5632
pFN21AE6673 5632 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2350 2356 2350
Size: 6 5626
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3442 278
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2975 1937
Size: 1038 4594
Acc65I G'GTAC_C
Cuts at: 2953 2953
Size: 5632
AclI AA'CG_TT
Cuts at: 1563 3759 4132 1563
Size: 2196 373 3063
Fragments arranged by size:
3063 2196 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2186 3695 5079 5347 1632
Size: 554 1509 1384 268 1917
Fragments arranged by size:
1917 1509 1384 554 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5435
AflIII A'CryG_T
Cuts at: 1203 4531 5508 1203
Size: 3328 977 1327
Fragments arranged by size:
3328 1327 977
AgeI A'CCGG_T
Cuts at: 4463 4463
Size: 5632
AhdI GACnn_n'nnGTC
Cuts at: 4361 4361
Size: 5632
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5632
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5632
AlwNI CAG_nnn'CTG
Cuts at: 3375 4947 3375
Size: 1572 4060
ApaI G_GGCC'C
Cuts at: 2033 2033
Size: 5632
ApaLI G'TGCA_C
Cuts at: 3689 4845 3689
Size: 1156 4476
ApoI r'AATT_y
Cuts at: 1493 2461 2941 3167 3319 5428 1493
Size: 968 480 226 152 2109 1697
Fragments arranged by size:
2109 1697 968 480 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4186 160
Size: 872 3154 1606
Fragments arranged by size:
3154 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
AvaI C'yCGr_G
Cuts at: 1870 1943 2675 2857 2881 2957 1870
Size: 73 732 182 24 76 4545
Fragments arranged by size:
4545 732 182 76 73 24
BamHI G'GATC_C
Cuts at: 2962 3329 2962
Size: 367 5265
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2250 2516 2765 2861 2953
Size: 325 217 627 463 266 249 96 92
Cuts at: 2953 4408 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 463 325 266 249 217
96 92
BanII G_rGCy'C
Cuts at: 1391 2012 2033 2106 2216 2854 2951 1391
Size: 621 21 73 110 638 97 4072
Fragments arranged by size:
4072 638 621 110 97 73 21
BbeI G_GCGC'C
Cuts at: 2769 2769
Size: 5632
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2779 928
Size: 971 880 3781
Fragments arranged by size:
3781 971 880
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2098 2132 3823 3857 1903
Size: 34 161 34 1691 34 3678
Fragments arranged by size:
3678 1691 161 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3524 4734 3524
Size: 1210 4422
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5632
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2541 2661 2832
Size: 107 122 71 631 605 869 120 171
Cuts at: 2832 2849 4243 136
Size: 17 1394 1525
Fragments arranged by size:
1525 1394 869 631 605 171 122 120
107 71 17
BglII A'GATC_T
Cuts at: 5627 5627
Size: 5632
BlpI GC'TnA_GC
Cuts at: 3049 3049
Size: 5632
Bme1580I G_kGCm'C
Cuts at: 1790 2033 2519 2639 2864 3693 4849 1790
Size: 243 486 120 225 829 1156 2573
Fragments arranged by size:
2573 1156 829 486 243 225 120
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5632
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3363 4321 442
Size: 416 53 83 399 24 1946 958 1753
Fragments arranged by size:
1946 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5632
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2612 4292 1239
Size: 1373 1680 2579
Fragments arranged by size:
2579 1680 1373
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3757 4622 4920 3757
Size: 865 298 4469
Fragments arranged by size:
4469 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4295 882
Size: 617 2796 2219
Fragments arranged by size:
2796 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3423 493
Size: 711 2219 2702
Fragments arranged by size:
2702 2219 711
BsaBI GATnn'nnATC
Cuts at: 5626 5626
Size: 5632
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2433 2766 3439 3821
Size: 53 83 186 936 900 333 673 382
Cuts at: 3821 4456 275
Size: 635 1451
Fragments arranged by size:
1451 936 900 673 635 382 333 186
83 53
BsaWI w'CCGG_w
Cuts at: 4065 4463 4737 4884 5295 4065
Size: 398 274 147 411 4402
Fragments arranged by size:
4402 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2717 2747 1178
Size: 30 165 30 1314 30 4063
Fragments arranged by size:
4063 1314 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2722 2933 1186
Size: 1536 211 3885
Fragments arranged by size:
3885 1536 211
BseYI C'CCAG_C
Cuts at: 2387 2707 4835 5331 2387
Size: 320 2128 496 2688
Fragments arranged by size:
2688 2128 496 320
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2384 1163
Size: 573 648 4411
Fragments arranged by size:
4411 648 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2052 3082 3843 4871 1540
Size: 275 186 51 1030 761 1028 2301
Fragments arranged by size:
2301 1030 1028 761 275 186 51
BsiHKAI G_wGCw'C
Cuts at: 2012 2106 3693 3778 4849 2012
Size: 94 1587 85 1071 2795
Fragments arranged by size:
2795 1587 1071 94 85
BsiWI C'GTAC_G
Cuts at: 2037 2037
Size: 5632
BsmI GAATG_Cn'
Cuts at: 3143 3236 3143
Size: 93 5539
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2033 2106 2216 2519 2639 2854
Size: 399 222 21 73 110 303 120 215
Cuts at: 2854 2864 2951 3693 3778 4849 1391
Size: 10 87 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 303 222 215 120
110 87 85 73 21 10
BspEI T'CCGG_A
Cuts at: 5295 5295
Size: 5632
BspHI T'CATG_A
Cuts at: 2193 3521 5251 2193
Size: 1328 1730 2574
Fragments arranged by size:
2574 1730 1328
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2350 2356 2987 844
Size: 1506 6 631 3489
Fragments arranged by size:
3489 1506 631 6
BsrBI CCG'CTC
Cuts at: 1394 1823 2235 2418 2458 2778 3519 1394
Size: 429 412 183 40 320 741 3507
Fragments arranged by size:
3507 741 429 412 320 183 40
BsrDI GCAATG_nn'
Cuts at: 65 1248 4127 4301 65
Size: 1183 2879 174 1396
Fragments arranged by size:
2879 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2064 2129 2799 2864 2924 4276 4463
Size: 110 254 65 670 65 60 1352 187
Cuts at: 4463 1700
Size: 2869
Fragments arranged by size:
2869 1352 670 254 187 110 65 65
60
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5632
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5632
BssSI C'ACGA_G
Cuts at: 2560 3692 4704 2560
Size: 1132 1012 3488
Fragments arranged by size:
3488 1132 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5632
BstBI TT'CG_AA
Cuts at: 4436 4436
Size: 5632
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5632
BstXI CCAn_nnnn'nTGG
Cuts at: 2659 2659
Size: 5632
BstYI r'GATC_y
Cuts at: 1972 2962 3329 3714 3731 5183 5269 5281 5627
Size: 990 367 385 17 1452 86 12 346
Cuts at: 5627 1972
Size: 1977
Fragments arranged by size:
1977 1452 990 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2286 2604 513
Size: 552 1221 318 3541
Fragments arranged by size:
3541 1221 552 318
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4893
Fragments arranged by size:
4893 683 56
BtsI GCAGTG_nn'
Cuts at: 3148 3942 3962 3148
Size: 794 20 4818
Fragments arranged by size:
4818 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5632
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5597
DraI TTT'AAA
Cuts at: 2936 2936
Size: 5632
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2432 4639 809
Size: 507 1116 2207 1802
Fragments arranged by size:
2207 1802 1116 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 2488 2779
Size: 54 1425 50 275 18 219 439 291
Cuts at: 2779 2841 3079 3968 5597 8
Size: 62 238 889 1629 43
Fragments arranged by size:
1629 1425 889 439 291 275 238 219
62 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2049 3079 1537
Size: 275 237 1030 4090
Fragments arranged by size:
4090 1030 275 237
EarI CTCTTCn'nnn_
Cuts at: 1353 2379 3562 1353
Size: 1026 1183 3423
Fragments arranged by size:
3423 1183 1026
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4219 4593 4739 387
Size: 1064 138 2630 374 146 1280
Fragments arranged by size:
2630 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2010 2104 2010
Size: 94 5538
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2186 2612 3695 4292 5079 5347 1239
Size: 393 554 426 1083 597 787 268 1524
Fragments arranged by size:
1524 1083 787 597 554 426 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2321 2321
Size: 5632
EcoO109I rG'GnC_Cy
Cuts at: 2029 2030 2161 2679 2890 2029
Size: 1 131 518 211 4771
Fragments arranged by size:
4771 518 211 131 1
EcoRI G'AATT_C
Cuts at: 2941 3319 2941
Size: 378 5254
FspI TGC'GCA
Cuts at: 4138 4138
Size: 5632
HaeII r_GCGC'y
Cuts at: 1678 2769 4779 1678
Size: 1091 2010 2531
Fragments arranged by size:
2531 2010 1091
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2554 2586 2717 2749 4273
Size: 32 135 32 982 32 131 32 1524
Cuts at: 4273 4305 4347 4379 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1524 982 135 131 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2976 3222 669
Size: 988 281 1038 246 3079
Fragments arranged by size:
3079 1038 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5632
HpaI GTT'AAC
Cuts at: 3222 3222
Size: 5632
KasI G'GCGC_C
Cuts at: 2765 2765
Size: 5632
KpnI G_GTAC'C
Cuts at: 2957 2957
Size: 5632
MfeI C'AATT_G
Cuts at: 3231 3231
Size: 5632
MluI A'CGCG_T
Cuts at: 5508 5508
Size: 5632
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2295 4746 4930 1305
Size: 990 2451 184 2007
Fragments arranged by size:
2451 2007 990 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2490 10
Size: 54 1425 343 658 3152
Fragments arranged by size:
3152 1425 658 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2192 2574 2694 3590 3949 4108 518
Size: 648 1026 382 120 896 359 159 2042
Fragments arranged by size:
2042 1026 896 648 382 359 159 120
MspA1I CmG'CkG
Cuts at: 1606 3048 3725 4873 5118 1606
Size: 1442 677 1148 245 2120
Fragments arranged by size:
2120 1442 1148 677 245
NaeI GCC'GGC
Cuts at: 2801 2926 2801
Size: 125 5507
NarI GG'CG_CC
Cuts at: 2766 2766
Size: 5632
NcoI C'CATG_G
Cuts at: 513 1065 2286 2604 513
Size: 552 1221 318 3541
Fragments arranged by size:
3541 1221 552 318
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5632
NgoMIV G'CCGG_C
Cuts at: 2799 2924 2799
Size: 125 5507
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5632
NotI GC'GGCC_GC
Cuts at: 3079 3079
Size: 5632
NspI r_CATG'y
Cuts at: 1132 1229 2990 4535 1132
Size: 97 1761 1545 2229
Fragments arranged by size:
2229 1761 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5632
PciI A'CATG_T
Cuts at: 4531 4531
Size: 5632
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5632
PmeI GTTT'AAAC
Cuts at: 2936 2936
Size: 5632
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3701 3733 5214 5246 3701
Size: 32 1481 32 4087
Fragments arranged by size:
4087 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2679 2890 2679
Size: 211 5421
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5632
PsiI TTA'TAA
Cuts at: 3202 3202
Size: 5632
PspOMI G'GGCC_C
Cuts at: 2029 2029
Size: 5632
PstI C_TGCA'G
Cuts at: 830 2415 2511 2748 2984 830
Size: 1585 96 237 236 3478
Fragments arranged by size:
3478 1585 237 236 96
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5632
SacI G_AGCT'C
Cuts at: 2012 2106 2012
Size: 94 5538
SalI G'TCGA_C
Cuts at: 1936 2974 1936
Size: 1038 4594
SanDI GG'GwC_CC
Cuts at: 2679 2679
Size: 5632
SbfI CC_TGCA'GG
Cuts at: 2984 2984
Size: 5632
ScaI AGT'ACT
Cuts at: 2441 2822 3880 5592 2441
Size: 381 1058 1712 2481
Fragments arranged by size:
2481 1712 1058 381
SfcI C'TryA_G
Cuts at: 826 1045 2204 2411 2507 2744 2980 4115 4796
Size: 219 1159 207 96 237 236 1135 681
Cuts at: 4796 4987 826
Size: 191 1471
Fragments arranged by size:
1471 1159 1135 681 237 236 219 207
191 96
SfoI GGC'GCC
Cuts at: 2767 2767
Size: 5632
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5632
SgrAI Cr'CCGG_yG
Cuts at: 1700 2864 1700
Size: 1164 4468
SmaI CCC'GGG
Cuts at: 1872 2677 2883 2959 1872
Size: 805 206 76 4545
Fragments arranged by size:
4545 805 206 76
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3736 4637 4899 820
Size: 197 926 1793 901 262 1553
Fragments arranged by size:
1793 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3423 493
Size: 2930 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5632
SphI G_CATG'C
Cuts at: 1132 2990 1132
Size: 1858 3774
SspI AAT'ATT
Cuts at: 5 52 3556 5
Size: 47 3504 2081
Fragments arranged by size:
3504 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5632
StyI C'CwwG_G
Cuts at: 513 1065 2286 2604 3071 513
Size: 552 1221 318 467 3074
Fragments arranged by size:
3074 1221 552 467 318
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3659 3676 3829 4014 4459 325
Size: 1255 2079 17 153 185 445 1498
Fragments arranged by size:
2079 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2439 2820 3878
Size: 274 80 33 51 1443 462 381 1058
Cuts at: 3878 5590 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1058 462 381 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5592 5624 5592
Size: 32 5600
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5632
XbaI T'CTAG_A
Cuts at: 2968 2968
Size: 5632
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5632
XmaI C'CCGG_G
Cuts at: 1870 2675 2881 2957 1870
Size: 805 206 76 4545
Fragments arranged by size:
4545 805 206 76
XmnI GAAnn'nnTTC
Cuts at: 3761 3761
Size: 5632
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3440 276
Size: 53 83 186 936 1906 2468
Fragments arranged by size:
2468 1906 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SacI SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI
PvuII RsrII SacII SapI SexAI SfiI SrfI SwaI
XcmI