Restriction map for
pFN21AE6669
(Circular) (Six-base) MAPSORT of: pFN21AE6669.seq Check: 4943 from: 1 to: 5593
pFN21AE6669 5593 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3403 278
Size: 53 83 186 936 1867 2468
Fragments arranged by size:
2468 1867 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2936 1937
Size: 999 4594
Acc65I G'GTAC_C
Cuts at: 2914 2914
Size: 5593
AclI AA'CG_TT
Cuts at: 1563 3720 4093 1563
Size: 2157 373 3063
Fragments arranged by size:
3063 2157 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3656 5040 5308 1632
Size: 2024 1384 268 1917
Fragments arranged by size:
2024 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5396
AflIII A'CryG_T
Cuts at: 1203 4492 5469 1203
Size: 3289 977 1327
Fragments arranged by size:
3289 1327 977
AgeI A'CCGG_T
Cuts at: 4424 4424
Size: 5593
AhdI GACnn_n'nnGTC
Cuts at: 2613 4322 2613
Size: 1709 3884
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5593
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5593
AlwNI CAG_nnn'CTG
Cuts at: 2332 3336 4908 2332
Size: 1004 1572 3017
Fragments arranged by size:
3017 1572 1004
ApaLI G'TGCA_C
Cuts at: 3650 4806 3650
Size: 1156 4437
ApoI r'AATT_y
Cuts at: 1493 2399 2902 3128 3280 5389 1493
Size: 906 503 226 152 2109 1697
Fragments arranged by size:
2109 1697 906 503 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4147 160
Size: 872 3115 1606
Fragments arranged by size:
3115 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5593
AvaI C'yCGr_G
Cuts at: 1870 1943 2018 2147 2918 1870
Size: 73 75 129 771 4545
Fragments arranged by size:
4545 771 129 75 73
BamHI G'GATC_C
Cuts at: 2923 3290 2923
Size: 367 5226
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2914 4369 618
Size: 325 217 627 1127 1455 1842
Fragments arranged by size:
1842 1455 1127 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2019 2148 2237 2912 1391
Size: 628 129 89 675 4072
Fragments arranged by size:
4072 675 628 129 89
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4622
BbvCI CC'TCA_GC
Cuts at: 2104 2104
Size: 5593
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3784 3818 1903
Size: 34 1847 34 3678
Fragments arranged by size:
3678 1847 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3485 4695 3485
Size: 1210 4383
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5593
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2061 4204 136
Size: 107 122 71 631 605 389 2143 1525
Fragments arranged by size:
2143 1525 631 605 389 122 107 71
BglII A'GATC_T
Cuts at: 5588 5588
Size: 5593
BlpI GC'TnA_GC
Cuts at: 2346 3010 2346
Size: 664 4929
Bme1580I G_kGCm'C
Cuts at: 1790 3654 4810 1790
Size: 1864 1156 2573
Fragments arranged by size:
2573 1864 1156
BmgBI CAC'GTC
Cuts at: 1318 2284 1318
Size: 966 4627
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3324 4282 442
Size: 416 53 83 399 24 1907 958 1753
Fragments arranged by size:
1907 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5593
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2221 2639 4253 1239
Size: 982 418 1614 2579
Fragments arranged by size:
2579 1614 982 418
Bpu10I CC'TnA_GC
Cuts at: 2104 2325 2104
Size: 221 5372
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3718 4583 4881 3718
Size: 865 298 4430
Fragments arranged by size:
4430 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2539 4256 882
Size: 617 1040 1717 2219
Fragments arranged by size:
2219 1717 1040 617
BsaAI yAC'GTr
Cuts at: 493 1204 3384 493
Size: 711 2180 2702
Fragments arranged by size:
2702 2180 711
BsaBI GATnn'nnATC
Cuts at: 5587 5587
Size: 5593
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3400 3782 4417 275
Size: 53 83 186 936 1867 382 635 1451
Fragments arranged by size:
1867 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4026 4424 4698 4845 5256 4026
Size: 398 274 147 411 4363
Fragments arranged by size:
4363 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5368
Fragments arranged by size:
5368 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2387 2444 1186
Size: 1201 57 4335
Fragments arranged by size:
4335 1201 57
BseYI C'CCAG_C
Cuts at: 4796 5292 4796
Size: 496 5097
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2825 1163
Size: 573 1089 3931
Fragments arranged by size:
3931 1089 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2322 3043 3804 4832 1540
Size: 275 186 321 721 761 1028 2301
Fragments arranged by size:
2301 1028 761 721 321 275 186
BsiHKAI G_wGCw'C
Cuts at: 2148 2237 3654 3739 4810 2148
Size: 89 1417 85 1071 2931
Fragments arranged by size:
2931 1417 1071 89 85
BsmI GAATG_Cn'
Cuts at: 2014 2501 3104 3197 2014
Size: 487 603 93 4410
Fragments arranged by size:
4410 603 487 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2019 2148 2237 2912 3654 3739 4810
Size: 399 229 129 89 675 742 85 1071
Cuts at: 4810 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 675 399 229 129 89
85
BspEI T'CCGG_A
Cuts at: 5256 5256
Size: 5593
BspHI T'CATG_A
Cuts at: 3482 5212 3482
Size: 1730 3863
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2445 2948 844
Size: 1601 503 3489
Fragments arranged by size:
3489 1601 503
BsrBI CCG'CTC
Cuts at: 1394 1823 2324 2485 3480 1394
Size: 429 501 161 995 3507
Fragments arranged by size:
3507 995 501 429 161
BsrDI GCAATG_nn'
Cuts at: 65 1248 4088 4262 65
Size: 1183 2840 174 1396
Fragments arranged by size:
2840 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2060 4237 4424 1700
Size: 110 250 2177 187 2869
Fragments arranged by size:
2869 2177 250 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5593
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5593
BssSI C'ACGA_G
Cuts at: 2369 3653 4665 2369
Size: 1284 1012 3297
Fragments arranged by size:
3297 1284 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5593
BstBI TT'CG_AA
Cuts at: 4397 4397
Size: 5593
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5593
BstXI CCAn_nnnn'nTGG
Cuts at: 2646 2646
Size: 5593
BstYI r'GATC_y
Cuts at: 1972 2865 2923 3290 3675 3692 5144 5230 5242
Size: 893 58 367 385 17 1452 86 12
Cuts at: 5242 5588 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 893 385 367 346 86 58
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5041
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4854
Fragments arranged by size:
4854 683 56
BtsI GCAGTG_nn'
Cuts at: 2233 2842 3109 3903 3923 2233
Size: 609 267 794 20 3903
Fragments arranged by size:
3903 794 609 267 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5593
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5558
DraI TTT'AAA
Cuts at: 2897 2897
Size: 5593
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4600 809
Size: 507 3284 1802
Fragments arranged by size:
3284 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2053 2319 3040
Size: 54 1425 50 275 18 223 266 721
Cuts at: 3040 3929 5558 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 721 275 266 223 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2319 3040 1537
Size: 275 507 721 4090
Fragments arranged by size:
4090 721 507 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2462 2475 2571 2856 3523 1353
Size: 1109 13 96 285 667 3423
Fragments arranged by size:
3423 1109 667 285 96 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4180 4554 4700 387
Size: 1064 138 2591 374 146 1280
Fragments arranged by size:
2591 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2146 2235 2146
Size: 89 5504
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2221 2639 3656 4253 5040 5308 1239
Size: 393 589 418 1017 597 787 268 1524
Fragments arranged by size:
1524 1017 787 597 589 418 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2663 2712 2663
Size: 49 5544
EcoRI G'AATT_C
Cuts at: 2399 2902 3280 2399
Size: 503 378 4712
Fragments arranged by size:
4712 503 378
FspI TGC'GCA
Cuts at: 4099 4099
Size: 5593
HaeII r_GCGC'y
Cuts at: 1678 4740 1678
Size: 3062 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2599 2631 4234 4266 4308
Size: 32 135 32 1027 32 1603 32 42
Cuts at: 4308 4340 1373
Size: 32 2626
Fragments arranged by size:
2626 1603 1027 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2937 3183 669
Size: 988 281 999 246 3079
Fragments arranged by size:
3079 999 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2692 748
Size: 1944 3649
HpaI GTT'AAC
Cuts at: 3183 3183
Size: 5593
KpnI G_GTAC'C
Cuts at: 2918 2918
Size: 5593
MfeI C'AATT_G
Cuts at: 3192 3192
Size: 5593
MluI A'CGCG_T
Cuts at: 5469 5469
Size: 5593
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2454 4707 4891 1305
Size: 1149 2253 184 2007
Fragments arranged by size:
2253 2007 1149 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3771
Fragments arranged by size:
3771 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2644 3551 3910 4069 518
Size: 648 1478 907 359 159 2042
Fragments arranged by size:
2042 1478 907 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2465 2508 3009 3686 4834 5079 1606
Size: 859 43 501 677 1148 245 2120
Fragments arranged by size:
2120 1148 859 677 501 245 43
NaeI GCC'GGC
Cuts at: 2062 2062
Size: 5593
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5041
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5593
NgoMIV G'CCGG_C
Cuts at: 2060 2060
Size: 5593
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5593
NotI GC'GGCC_GC
Cuts at: 2319 3040 2319
Size: 721 4872
NspI r_CATG'y
Cuts at: 1132 1229 2381 2951 4496 1132
Size: 97 1152 570 1545 2229
Fragments arranged by size:
2229 1545 1152 570 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5593
PciI A'CATG_T
Cuts at: 4492 4492
Size: 5593
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2206 1108
Size: 1098 4495
PmeI GTTT'AAAC
Cuts at: 2897 2897
Size: 5593
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3662 3694 5175 5207 3662
Size: 32 1481 32 4048
Fragments arranged by size:
4048 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2663 2712 2663
Size: 49 5544
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5593
PsiI TTA'TAA
Cuts at: 3163 3163
Size: 5593
PstI C_TGCA'G
Cuts at: 830 2228 2945 830
Size: 1398 717 3478
Fragments arranged by size:
3478 1398 717
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5593
PvuII CAG'CTG
Cuts at: 2465 2465
Size: 5593
SacI G_AGCT'C
Cuts at: 2148 2237 2148
Size: 89 5504
SalI G'TCGA_C
Cuts at: 1936 2935 1936
Size: 999 4594
SapI GCTCTTCn'nnn_
Cuts at: 2475 2571 2475
Size: 96 5497
SbfI CC_TGCA'GG
Cuts at: 2945 2945
Size: 5593
ScaI AGT'ACT
Cuts at: 3841 5553 3841
Size: 1712 3881
SexAI A'CCwGG_T
Cuts at: 2665 2665
Size: 5593
SfcI C'TryA_G
Cuts at: 826 1045 2224 2941 4076 4757 4948 826
Size: 219 1179 717 1135 681 191 1471
Fragments arranged by size:
1471 1179 1135 717 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5593
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5593
SmaI CCC'GGG
Cuts at: 1872 2920 1872
Size: 1048 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3697 4598 4860 820
Size: 197 926 1754 901 262 1553
Fragments arranged by size:
1754 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3384 493
Size: 2891 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5593
SphI G_CATG'C
Cuts at: 1132 2951 1132
Size: 1819 3774
SspI AAT'ATT
Cuts at: 5 52 2876 3517 5
Size: 47 2824 641 2081
Fragments arranged by size:
2824 2081 641 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5593
StyI C'CwwG_G
Cuts at: 513 1065 2715 3032 513
Size: 552 1650 317 3074
Fragments arranged by size:
3074 1650 552 317
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3620 3637 3790 3975 4420 325
Size: 1255 2040 17 153 185 445 1498
Fragments arranged by size:
2040 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3839 5551 96
Size: 274 80 33 51 1443 1862 1712 138
Fragments arranged by size:
1862 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5553 5585 5553
Size: 32 5561
Tth111I GACn'n_nGTC
Cuts at: 1316 2308 2667 1316
Size: 992 359 4242
Fragments arranged by size:
4242 992 359
XbaI T'CTAG_A
Cuts at: 2929 2929
Size: 5593
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2203 2705 2203
Size: 502 5091
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5593
XmaI C'CCGG_G
Cuts at: 1870 2918 1870
Size: 1048 4545
XmnI GAAnn'nnTTC
Cuts at: 3722 3722
Size: 5593
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3401 276
Size: 53 83 186 936 1867 2468
Fragments arranged by size:
2468 1867 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SfiI SfoI SrfI SwaI