Restriction map for
pFN21AE6668
(Circular) (Six-base) MAPSORT of: pFN21AE6668.seq Check: 1266 from: 1 to: 5593
pFN21AE6668 5593 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2690 3403 278
Size: 53 83 186 936 1154 713 2468
Fragments arranged by size:
2468 1154 936 713 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2936 1937
Size: 999 4594
Acc65I G'GTAC_C
Cuts at: 2465 2914 2465
Size: 449 5144
AclI AA'CG_TT
Cuts at: 1563 3720 4093 1563
Size: 2157 373 3063
Fragments arranged by size:
3063 2157 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3656 5040 5308 1632
Size: 2024 1384 268 1917
Fragments arranged by size:
2024 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5396
AflIII A'CryG_T
Cuts at: 1203 4492 5469 1203
Size: 3289 977 1327
Fragments arranged by size:
3289 1327 977
AgeI A'CCGG_T
Cuts at: 4424 4424
Size: 5593
AhdI GACnn_n'nnGTC
Cuts at: 4322 4322
Size: 5593
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5593
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5593
AlwNI CAG_nnn'CTG
Cuts at: 2347 3336 4908 2347
Size: 989 1572 3032
Fragments arranged by size:
3032 1572 989
ApaLI G'TGCA_C
Cuts at: 3650 4806 3650
Size: 1156 4437
ApoI r'AATT_y
Cuts at: 1493 2616 2902 3128 3280 5389 1493
Size: 1123 286 226 152 2109 1697
Fragments arranged by size:
2109 1697 1123 286 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4147 160
Size: 872 3115 1606
Fragments arranged by size:
3115 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5593
AvaI C'yCGr_G
Cuts at: 1870 1943 2918 1870
Size: 73 975 4545
Fragments arranged by size:
4545 975 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2449 2482 2449
Size: 33 5560
BamHI G'GATC_C
Cuts at: 2923 3290 2923
Size: 367 5226
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2465 2914 4369 618
Size: 325 217 627 678 449 1455 1842
Fragments arranged by size:
1842 1455 678 627 449 325 217
BanII G_rGCy'C
Cuts at: 1391 2263 2912 1391
Size: 872 649 4072
Fragments arranged by size:
4072 872 649
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4622
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2600 2634 2872 2906 3784 3818 1903
Size: 34 663 34 238 34 878 34 3678
Fragments arranged by size:
3678 878 663 238 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2310 3485 4695 2310
Size: 1175 1210 3208
Fragments arranged by size:
3208 1210 1175
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5593
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4204 136
Size: 107 122 71 631 605 2532 1525
Fragments arranged by size:
2532 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5588 5588
Size: 5593
BlpI GC'TnA_GC
Cuts at: 3010 3010
Size: 5593
Bme1580I G_kGCm'C
Cuts at: 1790 3654 4810 1790
Size: 1864 1156 2573
Fragments arranged by size:
2573 1864 1156
BmgBI CAC'GTC
Cuts at: 1318 2206 1318
Size: 888 4705
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3324 4282 442
Size: 416 53 83 399 24 1907 958 1753
Fragments arranged by size:
1907 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5593
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2110 4253 1239
Size: 871 2143 2579
Fragments arranged by size:
2579 2143 871
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3718 4583 4881 3718
Size: 865 298 4430
Fragments arranged by size:
4430 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4256 882
Size: 617 2757 2219
Fragments arranged by size:
2757 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2533 3384 493
Size: 711 1329 851 2702
Fragments arranged by size:
2702 1329 851 711
BsaBI GATnn'nnATC
Cuts at: 5587 5587
Size: 5593
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2687 3400 3782 4417
Size: 53 83 186 936 1154 713 382 635
Cuts at: 4417 275
Size: 1451
Fragments arranged by size:
1451 1154 936 713 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4026 4424 4698 4845 5256 4026
Size: 398 274 147 411 4363
Fragments arranged by size:
4363 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5368
Fragments arranged by size:
5368 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2120 1186
Size: 934 4659
BseYI C'CCAG_C
Cuts at: 4796 5292 4796
Size: 496 5097
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2122 1163
Size: 573 386 4634
Fragments arranged by size:
4634 573 386
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3043 3804 4832 1540
Size: 275 186 1042 761 1028 2301
Fragments arranged by size:
2301 1042 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3654 3739 4810 3654
Size: 85 1071 4437
Fragments arranged by size:
4437 1071 85
BsmI GAATG_Cn'
Cuts at: 3104 3197 3104
Size: 93 5500
BsmBI CGTCTCn'nnnn_
Cuts at: 2678 2678
Size: 5593
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2263 2912 3654 3739 4810 1391
Size: 399 473 649 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 649 473 399 85
BspEI T'CCGG_A
Cuts at: 5256 5256
Size: 5593
BspHI T'CATG_A
Cuts at: 2772 3482 5212 2772
Size: 710 1730 3153
Fragments arranged by size:
3153 1730 710
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2948 844
Size: 2104 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3480 1394
Size: 429 1657 3507
Fragments arranged by size:
3507 1657 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2307 4088 4262 65
Size: 1183 1059 1781 174 1396
Fragments arranged by size:
1781 1396 1183 1059 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4237 4424 1700
Size: 110 2427 187 2869
Fragments arranged by size:
2869 2427 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5593
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5593
BssSI C'ACGA_G
Cuts at: 3653 4665 3653
Size: 1012 4581
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5593
BstBI TT'CG_AA
Cuts at: 4397 4397
Size: 5593
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5593
BstXI CCAn_nnnn'nTGG
Cuts at: 2279 2279
Size: 5593
BstYI r'GATC_y
Cuts at: 1972 2097 2923 3290 3675 3692 5144 5230 5242
Size: 125 826 367 385 17 1452 86 12
Cuts at: 5242 5588 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 826 385 367 346 125 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2312 513
Size: 552 939 308 3794
Fragments arranged by size:
3794 939 552 308
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4854
Fragments arranged by size:
4854 683 56
BtsI GCAGTG_nn'
Cuts at: 3109 3903 3923 3109
Size: 794 20 4779
Fragments arranged by size:
4779 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5593
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5558
DraI TTT'AAA
Cuts at: 2897 2897
Size: 5593
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4600 809
Size: 507 3284 1802
Fragments arranged by size:
3284 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2264 3040 3929
Size: 54 1425 50 275 18 434 776 889
Cuts at: 3929 5558 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 776 434 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3040 1537
Size: 275 1228 4090
Fragments arranged by size:
4090 1228 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2469 2579 2585 3523 1353
Size: 1116 110 6 938 3423
Fragments arranged by size:
3423 1116 938 110 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4180 4554 4700 387
Size: 1064 138 2591 374 146 1280
Fragments arranged by size:
2591 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2110 3656 4253 5040 5308 1239
Size: 393 478 1546 597 787 268 1524
Fragments arranged by size:
1546 1524 787 597 478 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2151 2151
Size: 5593
EcoO109I rG'GnC_Cy
Cuts at: 2058 2602 2058
Size: 544 5049
EcoRI G'AATT_C
Cuts at: 2902 3280 2902
Size: 378 5215
FspI TGC'GCA
Cuts at: 4099 4099
Size: 5593
HaeII r_GCGC'y
Cuts at: 1678 4740 1678
Size: 3062 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2214 2246 4234 4266 4308
Size: 32 135 32 642 32 1988 32 42
Cuts at: 4308 4340 1373
Size: 32 2626
Fragments arranged by size:
2626 1988 642 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2937 3183 669
Size: 988 281 999 246 3079
Fragments arranged by size:
3079 999 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5593
HpaI GTT'AAC
Cuts at: 3183 3183
Size: 5593
KpnI G_GTAC'C
Cuts at: 2469 2918 2469
Size: 449 5144
MfeI C'AATT_G
Cuts at: 3192 3192
Size: 5593
MluI A'CGCG_T
Cuts at: 5469 5469
Size: 5593
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2257 4707 4891 1305
Size: 952 2450 184 2007
Fragments arranged by size:
2450 2007 952 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3771
Fragments arranged by size:
3771 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3551 3910 4069 518
Size: 648 2385 359 159 2042
Fragments arranged by size:
2385 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2344 3009 3686 4834 5079 1606
Size: 738 665 677 1148 245 2120
Fragments arranged by size:
2120 1148 738 677 665 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4102
Fragments arranged by size:
4102 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5593
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5593
NotI GC'GGCC_GC
Cuts at: 3040 3040
Size: 5593
NspI r_CATG'y
Cuts at: 1132 1229 2452 2951 4496 1132
Size: 97 1223 499 1545 2229
Fragments arranged by size:
2229 1545 1223 499 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5593
PciI A'CATG_T
Cuts at: 4492 4492
Size: 5593
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5593
PfoI T'CCnGG_A
Cuts at: 2679 2679
Size: 5593
PmeI GTTT'AAAC
Cuts at: 2897 2897
Size: 5593
PmlI CAC'GTG
Cuts at: 2533 2533
Size: 5593
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3662 3694 5175 5207 3662
Size: 32 1481 32 4048
Fragments arranged by size:
4048 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2058 2602 2058
Size: 544 5049
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5593
PsiI TTA'TAA
Cuts at: 3163 3163
Size: 5593
PstI C_TGCA'G
Cuts at: 830 2070 2945 830
Size: 1240 875 3478
Fragments arranged by size:
3478 1240 875
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5593
PvuII CAG'CTG
Cuts at: 2344 2344
Size: 5593
SalI G'TCGA_C
Cuts at: 1936 2935 1936
Size: 999 4594
SanDI GG'GwC_CC
Cuts at: 2058 2602 2058
Size: 544 5049
SbfI CC_TGCA'GG
Cuts at: 2945 2945
Size: 5593
ScaI AGT'ACT
Cuts at: 3841 5553 3841
Size: 1712 3881
SfcI C'TryA_G
Cuts at: 826 1045 2066 2730 2845 2941 4076 4757 4948
Size: 219 1021 664 115 96 1135 681 191
Cuts at: 4948 826
Size: 1471
Fragments arranged by size:
1471 1135 1021 681 664 219 191 115
96
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5593
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5593
SmaI CCC'GGG
Cuts at: 1872 2920 1872
Size: 1048 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3697 4598 4860 820
Size: 197 926 1754 901 262 1553
Fragments arranged by size:
1754 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3384 493
Size: 2891 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5593
SphI G_CATG'C
Cuts at: 1132 2951 1132
Size: 1819 3774
SspI AAT'ATT
Cuts at: 5 52 3517 5
Size: 47 3465 2081
Fragments arranged by size:
3465 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5593
StyI C'CwwG_G
Cuts at: 513 1065 2004 3032 513
Size: 552 939 1028 3074
Fragments arranged by size:
3074 1028 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3620 3637 3790 3975 4420 325
Size: 1255 2040 17 153 185 445 1498
Fragments arranged by size:
2040 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3839 5551 96
Size: 274 80 33 51 1443 1862 1712 138
Fragments arranged by size:
1862 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2634 2666 5553 5585 2634
Size: 32 2887 32 2642
Fragments arranged by size:
2887 2642 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5593
XbaI T'CTAG_A
Cuts at: 2929 2929
Size: 5593
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2358 2410 2746 2358
Size: 52 336 5205
Fragments arranged by size:
5205 336 52
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5593
XmaI C'CCGG_G
Cuts at: 1870 2918 1870
Size: 1048 4545
XmnI GAAnn'nnTTC
Cuts at: 3722 3722
Size: 5593
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2688 3401 276
Size: 53 83 186 936 1154 713 2468
Fragments arranged by size:
2468 1154 936 713 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI
SacII SapI SexAI SfiI SfoI SrfI SwaI