Restriction map for
pFN21AE6667
(Circular) (Six-base) MAPSORT of: pFN21AE6667.seq Check: 1264 from: 1 to: 5599
pFN21AE6667 5599 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3409 278
Size: 53 83 186 936 1873 2468
Fragments arranged by size:
2468 1873 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2942 1937
Size: 1005 4594
Acc65I G'GTAC_C
Cuts at: 2920 2920
Size: 5599
AclI AA'CG_TT
Cuts at: 1563 3726 4099 1563
Size: 2163 373 3063
Fragments arranged by size:
3063 2163 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2583 3662 5046 5314 1632
Size: 951 1079 1384 268 1917
Fragments arranged by size:
1917 1384 1079 951 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5402
AflIII A'CryG_T
Cuts at: 1203 4498 5475 1203
Size: 3295 977 1327
Fragments arranged by size:
3295 1327 977
AgeI A'CCGG_T
Cuts at: 4430 4430
Size: 5599
AhdI GACnn_n'nnGTC
Cuts at: 4328 4328
Size: 5599
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5599
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5599
AlwNI CAG_nnn'CTG
Cuts at: 3342 4914 3342
Size: 1572 4027
ApaLI G'TGCA_C
Cuts at: 3656 4812 3656
Size: 1156 4443
ApoI r'AATT_y
Cuts at: 1493 2195 2908 3134 3286 5395 1493
Size: 702 713 226 152 2109 1697
Fragments arranged by size:
2109 1697 713 702 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4153 160
Size: 872 3121 1606
Fragments arranged by size:
3121 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5599
AvaI C'yCGr_G
Cuts at: 1870 1943 2924 1870
Size: 73 981 4545
Fragments arranged by size:
4545 981 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2131 2164 2131
Size: 33 5566
BamHI G'GATC_C
Cuts at: 2929 3296 2929
Size: 367 5232
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2871 2920 4375 618
Size: 325 217 627 1084 49 1455 1842
Fragments arranged by size:
1842 1455 1084 627 325 217 49
BanII G_rGCy'C
Cuts at: 1391 2136 2551 2918 1391
Size: 745 415 367 4072
Fragments arranged by size:
4072 745 415 367
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2436 928
Size: 971 537 4091
Fragments arranged by size:
4091 971 537
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2785 2819 3790 3824 1903
Size: 34 848 34 971 34 3678
Fragments arranged by size:
3678 971 848 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2658 3491 4701 2658
Size: 833 1210 3556
Fragments arranged by size:
3556 1210 833
BclI T'GATC_A
Cuts at: 1548 2331 1548
Size: 783 4816
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4210 136
Size: 107 122 71 631 605 2538 1525
Fragments arranged by size:
2538 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5594 5594
Size: 5599
BlpI GC'TnA_GC
Cuts at: 3016 3016
Size: 5599
Bme1580I G_kGCm'C
Cuts at: 1790 3660 4816 1790
Size: 1870 1156 2573
Fragments arranged by size:
2573 1870 1156
BmgBI CAC'GTC
Cuts at: 1318 2626 1318
Size: 1308 4291
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3330 4288 442
Size: 416 53 83 399 24 1913 958 1753
Fragments arranged by size:
1913 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5599
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2764 2764
Size: 5599
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2541 2565 4259 1239
Size: 1302 24 1694 2579
Fragments arranged by size:
2579 1694 1302 24
Bpu10I CC'TnA_GC
Cuts at: 2706 2706
Size: 5599
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2868 3724 4589 4887 2868
Size: 856 865 298 3580
Fragments arranged by size:
3580 865 856 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4262 882
Size: 617 2763 2219
Fragments arranged by size:
2763 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3390 493
Size: 711 2186 2702
Fragments arranged by size:
2702 2186 711
BsaBI GATnn'nnATC
Cuts at: 5593 5593
Size: 5599
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3406 3788 4423 275
Size: 53 83 186 936 1873 382 635 1451
Fragments arranged by size:
1873 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4032 4430 4704 4851 5262 4032
Size: 398 274 147 411 4369
Fragments arranged by size:
4369 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5374
Fragments arranged by size:
5374 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2438 1186
Size: 1252 4347
BseYI C'CCAG_C
Cuts at: 4802 5298 4802
Size: 496 5103
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2151 1163
Size: 573 415 4611
Fragments arranged by size:
4611 573 415
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3049 3810 4838 1540
Size: 275 186 1048 761 1028 2301
Fragments arranged by size:
2301 1048 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3660 3745 4816 3660
Size: 85 1071 4443
Fragments arranged by size:
4443 1071 85
BsmI GAATG_Cn'
Cuts at: 3110 3203 3110
Size: 93 5506
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2136 2551 2918 3660 3745 4816 1391
Size: 399 346 415 367 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 415 399 367 346 85
BspEI T'CCGG_A
Cuts at: 5262 5262
Size: 5599
BspHI T'CATG_A
Cuts at: 3488 5218 3488
Size: 1730 3869
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2954 844
Size: 2110 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3486 1394
Size: 429 1663 3507
Fragments arranged by size:
3507 1663 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4094 4268 65
Size: 1183 2846 174 1396
Fragments arranged by size:
2846 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4243 4430 1700
Size: 110 2433 187 2869
Fragments arranged by size:
2869 2433 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5599
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5599
BssSI C'ACGA_G
Cuts at: 3659 4671 3659
Size: 1012 4587
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5599
BstBI TT'CG_AA
Cuts at: 4403 4403
Size: 5599
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5599
BstYI r'GATC_y
Cuts at: 1972 2929 3296 3681 3698 5150 5236 5248 5594
Size: 957 367 385 17 1452 86 12 346
Cuts at: 5594 1972
Size: 1977
Fragments arranged by size:
1977 1452 957 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5047
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4860
Fragments arranged by size:
4860 683 56
BtsI GCAGTG_nn'
Cuts at: 2284 2370 3115 3909 3929 2284
Size: 86 745 794 20 3954
Fragments arranged by size:
3954 794 745 86 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5599
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5564
DraI TTT'AAA
Cuts at: 2903 2903
Size: 5599
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4606 809
Size: 507 3290 1802
Fragments arranged by size:
3290 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3046 3935 5564
Size: 54 1425 50 275 18 1216 889 1629
Cuts at: 5564 8
Size: 43
Fragments arranged by size:
1629 1425 1216 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3046 1537
Size: 275 1234 4090
Fragments arranged by size:
4090 1234 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2101 2106 3529 1353
Size: 748 5 1423 3423
Fragments arranged by size:
3423 1423 748 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4186 4560 4706 387
Size: 1064 138 2597 374 146 1280
Fragments arranged by size:
2597 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2541 2565 2583 3662 4259 5046 5314
Size: 393 909 24 18 1079 597 787 268
Cuts at: 5314 1239
Size: 1524
Fragments arranged by size:
1524 1079 909 787 597 393 268 24
18
EcoNI CCTnn'n_nnAGG
Cuts at: 2410 2410
Size: 5599
EcoO109I rG'GnC_Cy
Cuts at: 2254 2254
Size: 5599
EcoRI G'AATT_C
Cuts at: 2908 3286 2908
Size: 378 5221
FspI TGC'GCA
Cuts at: 4105 4105
Size: 5599
HaeII r_GCGC'y
Cuts at: 1678 2400 4746 1678
Size: 722 2346 2531
Fragments arranged by size:
2531 2346 722
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2072 2104 2555 2587 4240
Size: 32 135 32 500 32 451 32 1653
Cuts at: 4240 4272 4314 4346 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1653 500 451 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2943 3189 669
Size: 988 281 1005 246 3079
Fragments arranged by size:
3079 1005 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5599
HpaI GTT'AAC
Cuts at: 3189 3189
Size: 5599
KpnI G_GTAC'C
Cuts at: 2924 2924
Size: 5599
MfeI C'AATT_G
Cuts at: 3198 3198
Size: 5599
MluI A'CGCG_T
Cuts at: 5475 5475
Size: 5599
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2748 2874 4713 4897 1305
Size: 1443 126 1839 184 2007
Fragments arranged by size:
2007 1839 1443 184 126
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3777
Fragments arranged by size:
3777 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2718 2798 3557 3916 4075 518
Size: 648 1552 80 759 359 159 2042
Fragments arranged by size:
2042 1552 759 648 359 159 80
MspA1I CmG'CkG
Cuts at: 1606 3015 3692 4840 5085 1606
Size: 1409 677 1148 245 2120
Fragments arranged by size:
2120 1409 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5047
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5599
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5599
NotI GC'GGCC_GC
Cuts at: 3046 3046
Size: 5599
NspI r_CATG'y
Cuts at: 1132 1229 2476 2957 4502 1132
Size: 97 1247 481 1545 2229
Fragments arranged by size:
2229 1545 1247 481 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5599
PciI A'CATG_T
Cuts at: 4498 4498
Size: 5599
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5599
PmeI GTTT'AAAC
Cuts at: 2903 2903
Size: 5599
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3668 3700 5181 5213 3668
Size: 32 1481 32 4054
Fragments arranged by size:
4054 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2254 2254
Size: 5599
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5599
PsiI TTA'TAA
Cuts at: 3169 3169
Size: 5599
PstI C_TGCA'G
Cuts at: 830 2951 830
Size: 2121 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5599
SalI G'TCGA_C
Cuts at: 1936 2941 1936
Size: 1005 4594
SbfI CC_TGCA'GG
Cuts at: 2951 2951
Size: 5599
ScaI AGT'ACT
Cuts at: 3847 5559 3847
Size: 1712 3887
SexAI A'CCwGG_T
Cuts at: 2895 2895
Size: 5599
SfcI C'TryA_G
Cuts at: 826 1045 2947 4082 4763 4954 826
Size: 219 1902 1135 681 191 1471
Fragments arranged by size:
1902 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5599
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5599
SmaI CCC'GGG
Cuts at: 1872 2926 1872
Size: 1054 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2883 3703 4604 4866 820
Size: 197 926 940 820 901 262 1553
Fragments arranged by size:
1553 940 926 901 820 262 197
SnaBI TAC'GTA
Cuts at: 493 3390 493
Size: 2897 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5599
SphI G_CATG'C
Cuts at: 1132 2957 1132
Size: 1825 3774
SspI AAT'ATT
Cuts at: 5 52 3523 5
Size: 47 3471 2081
Fragments arranged by size:
3471 2081 47
StuI AGG'CCT
Cuts at: 1517 2826 1517
Size: 1309 4290
StyI C'CwwG_G
Cuts at: 513 1065 3038 513
Size: 552 1973 3074
Fragments arranged by size:
3074 1973 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3626 3643 3796 3981 4426 325
Size: 1255 2046 17 153 185 445 1498
Fragments arranged by size:
2046 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3845 5557 96
Size: 274 80 33 51 1443 1868 1712 138
Fragments arranged by size:
1868 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5559 5591 5559
Size: 32 5567
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5599
XbaI T'CTAG_A
Cuts at: 2935 2935
Size: 5599
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5599
XmaI C'CCGG_G
Cuts at: 1870 2924 1870
Size: 1054 4545
XmnI GAAnn'nnTTC
Cuts at: 2492 3728 2492
Size: 1236 4363
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3407 276
Size: 53 83 186 936 1873 2468
Fragments arranged by size:
2468 1873 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI XcmI