Restriction map for
pFN21AE6663
(Circular) (Six-base) MAPSORT of: pFN21AE6663.seq Check: 1793 from: 1 to: 5560
pFN21AE6663 5560 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2526 2526
Size: 5560
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3370 278
Size: 53 83 186 936 1834 2468
Fragments arranged by size:
2468 1834 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2903 1937
Size: 966 4594
Acc65I G'GTAC_C
Cuts at: 2776 2881 2776
Size: 105 5455
AclI AA'CG_TT
Cuts at: 1563 3687 4060 1563
Size: 2124 373 3063
Fragments arranged by size:
3063 2124 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3623 5007 5275 1632
Size: 1991 1384 268 1917
Fragments arranged by size:
1991 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5363
AflIII A'CryG_T
Cuts at: 1203 2466 4459 5436 1203
Size: 1263 1993 977 1327
Fragments arranged by size:
1993 1327 1263 977
AgeI A'CCGG_T
Cuts at: 4391 4391
Size: 5560
AhdI GACnn_n'nnGTC
Cuts at: 4289 4289
Size: 5560
AleI CACnn'nnGTG
Cuts at: 1166 2240 2555 1166
Size: 1074 315 4171
Fragments arranged by size:
4171 1074 315
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2339 1184
Size: 1155 4405
AlwNI CAG_nnn'CTG
Cuts at: 2657 3303 4875 2657
Size: 646 1572 3342
Fragments arranged by size:
3342 1572 646
ApaLI G'TGCA_C
Cuts at: 3617 4773 3617
Size: 1156 4404
ApoI r'AATT_y
Cuts at: 1493 2869 3095 3247 5356 1493
Size: 1376 226 152 2109 1697
Fragments arranged by size:
2109 1697 1376 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4114 160
Size: 872 3082 1606
Fragments arranged by size:
3082 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5560
AvaI C'yCGr_G
Cuts at: 1870 1943 2503 2885 1870
Size: 73 560 382 4545
Fragments arranged by size:
4545 560 382 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2760 2768 2793 2801 2760
Size: 8 25 8 5519
Fragments arranged by size:
5519 25 8 8
BamHI G'GATC_C
Cuts at: 2890 3257 2890
Size: 367 5193
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2776 2810 2881 4336 618
Size: 325 217 627 989 34 71 1455 1842
Fragments arranged by size:
1842 1455 989 627 325 217 71 34
BanII G_rGCy'C
Cuts at: 1391 2285 2340 2820 2879 1391
Size: 894 55 480 59 4072
Fragments arranged by size:
4072 894 480 59 55
BbeI G_GCGC'C
Cuts at: 2814 2814
Size: 5560
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4589
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3751 3785 1903
Size: 34 1814 34 3678
Fragments arranged by size:
3678 1814 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3452 4662 3452
Size: 1210 4350
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5560
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2585 4171 136
Size: 107 122 71 631 605 913 1586 1525
Fragments arranged by size:
1586 1525 913 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5555 5555
Size: 5560
BlpI GC'TnA_GC
Cuts at: 2819 2840 2977 2819
Size: 21 137 5402
Fragments arranged by size:
5402 137 21
Bme1580I G_kGCm'C
Cuts at: 1790 2697 3621 4777 1790
Size: 907 924 1156 2573
Fragments arranged by size:
2573 1156 924 907
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5560
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2180 3291 4249
Size: 416 53 83 399 24 763 1111 958
Cuts at: 4249 442
Size: 1753
Fragments arranged by size:
1753 1111 958 763 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5560
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2304 2304
Size: 5560
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2299 2819 4220 1239
Size: 1060 520 1401 2579
Fragments arranged by size:
2579 1401 1060 520
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3685 4550 4848 3685
Size: 865 298 4397
Fragments arranged by size:
4397 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2531 4223 882
Size: 617 1032 1692 2219
Fragments arranged by size:
2219 1692 1032 617
BsaAI yAC'GTr
Cuts at: 493 1204 3351 493
Size: 711 2147 2702
Fragments arranged by size:
2702 2147 711
BsaBI GATnn'nnATC
Cuts at: 5554 5554
Size: 5560
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 2075 2811 3367
Size: 53 83 186 936 479 63 736 556
Cuts at: 3367 3749 4384 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 736 635 556 479 382 186
83 63 53
BsaWI w'CCGG_w
Cuts at: 3993 4391 4665 4812 5223 3993
Size: 398 274 147 411 4330
Fragments arranged by size:
4330 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5335
Fragments arranged by size:
5335 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2216 2460 1186
Size: 1030 244 4286
Fragments arranged by size:
4286 1030 244
BseYI C'CCAG_C
Cuts at: 2026 2146 2215 4763 5259 2026
Size: 120 69 2548 496 2327
Fragments arranged by size:
2548 2327 496 120 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2693 1163
Size: 573 957 4030
Fragments arranged by size:
4030 957 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2241 2404 3010 3771 4799 1540
Size: 275 186 240 163 606 761 1028 2301
Fragments arranged by size:
2301 1028 761 606 275 240 186 163
BsiHKAI G_wGCw'C
Cuts at: 2139 2841 3621 3706 4777 2139
Size: 702 780 85 1071 2922
Fragments arranged by size:
2922 1071 780 702 85
BsmI GAATG_Cn'
Cuts at: 3071 3164 3071
Size: 93 5467
BsmBI CGTCTCn'nnnn_
Cuts at: 2352 2491 2352
Size: 139 5421
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2139 2285 2340 2697 2820 2841 2879
Size: 399 349 146 55 357 123 21 38
Cuts at: 2879 3621 3706 4777 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 357 349 146 123
85 55 38 21
BspEI T'CCGG_A
Cuts at: 5223 5223
Size: 5560
BspHI T'CATG_A
Cuts at: 3449 5179 3449
Size: 1730 3830
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2526 2915 844
Size: 1682 389 3489
Fragments arranged by size:
3489 1682 389
BsrBI CCG'CTC
Cuts at: 1394 1823 2108 2288 3447 1394
Size: 429 285 180 1159 3507
Fragments arranged by size:
3507 1159 429 285 180
BsrDI GCAATG_nn'
Cuts at: 65 1248 2207 2604 4055 4229 65
Size: 1183 959 397 1451 174 1396
Fragments arranged by size:
1451 1396 1183 959 397 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2399 4204 4391 1700
Size: 110 589 1805 187 2869
Fragments arranged by size:
2869 1805 589 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5560
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5560
BssSI C'ACGA_G
Cuts at: 3620 4632 3620
Size: 1012 4548
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2143 794
Size: 1349 4211
BstBI TT'CG_AA
Cuts at: 4364 4364
Size: 5560
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5560
BstYI r'GATC_y
Cuts at: 1972 2113 2725 2890 3257 3642 3659 5111 5197
Size: 141 612 165 367 385 17 1452 86
Cuts at: 5197 5209 5555 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 612 385 367 346 165 141
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2199 2199
Size: 5560
BtgI C'CryG_G
Cuts at: 513 1065 2004 2713 513
Size: 552 939 709 3360
Fragments arranged by size:
3360 939 709 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4821
Fragments arranged by size:
4821 683 56
BtsI GCAGTG_nn'
Cuts at: 2610 2747 3076 3870 3890 2610
Size: 137 329 794 20 4280
Fragments arranged by size:
4280 794 329 137 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5560
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5525
DraI TTT'AAA
Cuts at: 2864 2864
Size: 5560
DraIII CAC_nnn'GTG
Cuts at: 2143 2143
Size: 5560
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2483 4567 809
Size: 507 1167 2084 1802
Fragments arranged by size:
2084 1802 1167 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2118 2238 2401
Size: 54 1425 50 275 18 288 120 163
Cuts at: 2401 3007 3896 5525 8
Size: 606 889 1629 43
Fragments arranged by size:
1629 1425 889 606 288 275 163 120
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2238 2401 3007 1537
Size: 275 426 163 606 4090
Fragments arranged by size:
4090 606 426 275 163
EarI CTCTTCn'nnn_
Cuts at: 1353 2399 3490 1353
Size: 1046 1091 3423
Fragments arranged by size:
3423 1091 1046
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2477 4147 4521 4667 387
Size: 1064 138 888 1670 374 146 1280
Fragments arranged by size:
1670 1280 1064 888 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2299 2819 3623 4220 5007 5275 1239
Size: 393 667 520 804 597 787 268 1524
Fragments arranged by size:
1524 804 787 667 597 520 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2507 2543 2507
Size: 36 5524
EcoRI G'AATT_C
Cuts at: 2869 3247 2869
Size: 378 5182
FspI TGC'GCA
Cuts at: 4066 4066
Size: 5560
HaeII r_GCGC'y
Cuts at: 1678 2814 4707 1678
Size: 1136 1893 2531
Fragments arranged by size:
2531 1893 1136
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2439 2471 4201 4233 4275
Size: 32 135 32 867 32 1730 32 42
Cuts at: 4275 4307 1373
Size: 32 2626
Fragments arranged by size:
2626 1730 867 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2904 3150 669
Size: 988 281 966 246 3079
Fragments arranged by size:
3079 988 966 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5560
HpaI GTT'AAC
Cuts at: 3150 3150
Size: 5560
KasI G'GCGC_C
Cuts at: 2810 2810
Size: 5560
KpnI G_GTAC'C
Cuts at: 2780 2885 2780
Size: 105 5455
MfeI C'AATT_G
Cuts at: 3159 3159
Size: 5560
MluI A'CGCG_T
Cuts at: 5436 5436
Size: 5560
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4674 4858 1305
Size: 3369 184 2007
Fragments arranged by size:
3369 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2120 10
Size: 54 1425 343 288 3450
Fragments arranged by size:
3450 1425 343 288 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2240 2555 2622 3518 3877 4036 518
Size: 648 1074 315 67 896 359 159 2042
Fragments arranged by size:
2042 1074 896 648 359 315 159 67
MspA1I CmG'CkG
Cuts at: 1606 2034 2182 2215 2224 2654 2976 3653 4801
Size: 428 148 33 9 430 322 677 1148
Cuts at: 4801 5046 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 430 428 322 245 148
33 9
NarI GG'CG_CC
Cuts at: 2811 2811
Size: 5560
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4069
Fragments arranged by size:
4069 939 552
NdeI CA'TA_TG
Cuts at: 387 2623 387
Size: 2236 3324
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5560
NotI GC'GGCC_GC
Cuts at: 3007 3007
Size: 5560
NspI r_CATG'y
Cuts at: 1132 1229 2918 4463 1132
Size: 97 1689 1545 2229
Fragments arranged by size:
2229 1689 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5560
PciI A'CATG_T
Cuts at: 4459 4459
Size: 5560
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5560
PfoI T'CCnGG_A
Cuts at: 2472 2472
Size: 5560
PmeI GTTT'AAAC
Cuts at: 2864 2864
Size: 5560
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3629 3661 5142 5174 3629
Size: 32 1481 32 4015
Fragments arranged by size:
4015 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2507 2543 2507
Size: 36 5524
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5560
PsiI TTA'TAA
Cuts at: 3130 3130
Size: 5560
PstI C_TGCA'G
Cuts at: 830 2754 2912 830
Size: 1924 158 3478
Fragments arranged by size:
3478 1924 158
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5560
PvuII CAG'CTG
Cuts at: 2182 2654 2182
Size: 472 5088
SalI G'TCGA_C
Cuts at: 1936 2902 1936
Size: 966 4594
SanDI GG'GwC_CC
Cuts at: 2507 2507
Size: 5560
SbfI CC_TGCA'GG
Cuts at: 2912 2912
Size: 5560
ScaI AGT'ACT
Cuts at: 3808 5520 3808
Size: 1712 3848
SfcI C'TryA_G
Cuts at: 826 1045 2177 2750 2908 4043 4724 4915 826
Size: 219 1132 573 158 1135 681 191 1471
Fragments arranged by size:
1471 1135 1132 681 573 219 191 158
SfoI GGC'GCC
Cuts at: 2812 2812
Size: 5560
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5560
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5560
SmaI CCC'GGG
Cuts at: 1872 2887 1872
Size: 1015 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3664 4565 4827 820
Size: 197 926 1721 901 262 1553
Fragments arranged by size:
1721 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3351 493
Size: 2858 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5560
SphI G_CATG'C
Cuts at: 1132 2918 1132
Size: 1786 3774
SspI AAT'ATT
Cuts at: 5 52 3484 5
Size: 47 3432 2081
Fragments arranged by size:
3432 2081 47
StuI AGG'CCT
Cuts at: 1517 2421 1517
Size: 904 4656
StyI C'CwwG_G
Cuts at: 513 1065 2004 2248 2999 513
Size: 552 939 244 751 3074
Fragments arranged by size:
3074 939 751 552 244
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2634 3587 3604 3757 3942 4387 325
Size: 1255 1054 953 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1054 953 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3806 5518 96
Size: 274 80 33 51 1443 1829 1712 138
Fragments arranged by size:
1829 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5520 5552 5520
Size: 32 5528
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5560
XbaI T'CTAG_A
Cuts at: 2896 2896
Size: 5560
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5560
XmaI C'CCGG_G
Cuts at: 1870 2885 1870
Size: 1015 4545
XmnI GAAnn'nnTTC
Cuts at: 3689 3689
Size: 5560
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3368 276
Size: 53 83 186 936 1834 2468
Fragments arranged by size:
2468 1834 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbvCI BfrBI Bpu10I
BsiWI BstXI BstZ17I EcoICRI EcoNI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SapI SexAI SfiI
SrfI SwaI XcmI