Restriction map for
pFN21AE6661
(Circular) (Six-base) MAPSORT of: pFN21AE6661.seq Check: 3108 from: 1 to: 5527
pFN21AE6661 5527 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2070 2070
Size: 5527
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3337 278
Size: 53 83 186 936 1801 2468
Fragments arranged by size:
2468 1801 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2870 1937
Size: 933 4594
Acc65I G'GTAC_C
Cuts at: 2492 2848 2492
Size: 356 5171
AclI AA'CG_TT
Cuts at: 1563 3654 4027 1563
Size: 2091 373 3063
Fragments arranged by size:
3063 2091 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2678 2691 3590 4974 5242 1632
Size: 1046 13 899 1384 268 1917
Fragments arranged by size:
1917 1384 1046 899 268 13
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5330
AflIII A'CryG_T
Cuts at: 1203 4426 5403 1203
Size: 3223 977 1327
Fragments arranged by size:
3223 1327 977
AgeI A'CCGG_T
Cuts at: 4358 4358
Size: 5527
AhdI GACnn_n'nnGTC
Cuts at: 4256 4256
Size: 5527
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5527
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5527
AlwNI CAG_nnn'CTG
Cuts at: 3270 4842 3270
Size: 1572 3955
ApaI G_GGCC'C
Cuts at: 2050 2050
Size: 5527
ApaLI G'TGCA_C
Cuts at: 3584 4740 3584
Size: 1156 4371
ApoI r'AATT_y
Cuts at: 1493 2836 3062 3214 5323 1493
Size: 1343 226 152 2109 1697
Fragments arranged by size:
2109 1697 1343 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4081 160
Size: 872 3049 1606
Fragments arranged by size:
3049 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5527
AvaI C'yCGr_G
Cuts at: 1870 1943 2852 1870
Size: 73 909 4545
Fragments arranged by size:
4545 909 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2513 2546 2513
Size: 33 5494
BamHI G'GATC_C
Cuts at: 2857 3224 2857
Size: 367 5160
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2009 2492 2731 2848 4303
Size: 325 217 627 222 483 239 117 1455
Cuts at: 4303 618
Size: 1842
Fragments arranged by size:
1842 1455 627 483 325 239 222 217
117
BanII G_rGCy'C
Cuts at: 1391 2050 2846 1391
Size: 659 796 4072
Fragments arranged by size:
4072 796 659
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4556
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3718 3752 1903
Size: 34 1781 34 3678
Fragments arranged by size:
3678 1781 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2153 3419 4629 2153
Size: 1266 1210 3051
Fragments arranged by size:
3051 1266 1210
BclI T'GATC_A
Cuts at: 1548 2379 1548
Size: 831 4696
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2740 4138 136
Size: 107 122 71 631 605 1068 1398 1525
Fragments arranged by size:
1525 1398 1068 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5522 5522
Size: 5527
BlpI GC'TnA_GC
Cuts at: 2944 2944
Size: 5527
Bme1580I G_kGCm'C
Cuts at: 1790 2012 2050 2111 2542 3588 4744 1790
Size: 222 38 61 431 1046 1156 2573
Fragments arranged by size:
2573 1156 1046 431 222 61 38
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5527
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2095 2452 3258
Size: 416 53 83 399 24 678 357 806
Cuts at: 3258 4216 442
Size: 958 1753
Fragments arranged by size:
1753 958 806 678 416 399 357 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5527
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4187 1239
Size: 2948 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3652 4517 4815 3652
Size: 865 298 4364
Fragments arranged by size:
4364 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4190 882
Size: 617 2691 2219
Fragments arranged by size:
2691 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3318 493
Size: 711 2114 2702
Fragments arranged by size:
2702 2114 711
BsaBI GATnn'nnATC
Cuts at: 5521 5521
Size: 5527
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3334 3716 4351 275
Size: 53 83 186 936 1801 382 635 1451
Fragments arranged by size:
1801 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3960 4358 4632 4779 5190 3960
Size: 398 274 147 411 4297
Fragments arranged by size:
4297 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5302
Fragments arranged by size:
5302 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2141 1186
Size: 955 4572
BseYI C'CCAG_C
Cuts at: 4730 5226 4730
Size: 496 5031
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2042 2349 1163
Size: 573 306 307 4341
Fragments arranged by size:
4341 573 307 306
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2977 3738 4766 1540
Size: 275 186 976 761 1028 2301
Fragments arranged by size:
2301 1028 976 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3588 3673 4744 3588
Size: 85 1071 4371
Fragments arranged by size:
4371 1071 85
BsmI GAATG_Cn'
Cuts at: 2222 2703 3038 3131 2222
Size: 481 335 93 4618
Fragments arranged by size:
4618 481 335 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2050 2111 2542 2846 3588 3673
Size: 399 222 38 61 431 304 742 85
Cuts at: 3673 4744 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 431 399 304 222 85
61 38
BspEI T'CCGG_A
Cuts at: 5190 5190
Size: 5527
BspHI T'CATG_A
Cuts at: 2134 3416 5146 2134
Size: 1282 1730 2515
Fragments arranged by size:
2515 1730 1282
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2070 2085 2882 844
Size: 1226 15 797 3489
Fragments arranged by size:
3489 1226 797 15
BsrBI CCG'CTC
Cuts at: 1394 1823 3414 1394
Size: 429 1591 3507
Fragments arranged by size:
3507 1591 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2382 4022 4196 65
Size: 1183 1134 1640 174 1396
Fragments arranged by size:
1640 1396 1183 1134 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4171 4358 1700
Size: 110 2361 187 2869
Fragments arranged by size:
2869 2361 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5527
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5527
BssSI C'ACGA_G
Cuts at: 2723 3587 4599 2723
Size: 864 1012 3651
Fragments arranged by size:
3651 1012 864
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5527
BstBI TT'CG_AA
Cuts at: 4331 4331
Size: 5527
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5527
BstYI r'GATC_y
Cuts at: 1972 2857 3224 3609 3626 5078 5164 5176 5522
Size: 885 367 385 17 1452 86 12 346
Cuts at: 5522 1972
Size: 1977
Fragments arranged by size:
1977 1452 885 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4036
Fragments arranged by size:
4036 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4788
Fragments arranged by size:
4788 683 56
BtsI GCAGTG_nn'
Cuts at: 3043 3837 3857 3043
Size: 794 20 4713
Fragments arranged by size:
4713 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5527
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2583 2618 529
Size: 35 2019 35 3438
Fragments arranged by size:
3438 2019 35 35
DraI TTT'AAA
Cuts at: 2831 2831
Size: 5527
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4534 809
Size: 507 3218 1802
Fragments arranged by size:
3218 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2974 3863
Size: 54 1425 50 275 18 195 949 889
Cuts at: 3863 5492 8
Size: 1629 43
Fragments arranged by size:
1629 1425 949 889 275 195 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2974 1537
Size: 275 1162 4090
Fragments arranged by size:
4090 1162 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2314 2697 3457 1353
Size: 961 383 760 3423
Fragments arranged by size:
3423 961 760 383
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4114 4488 4634 387
Size: 1064 138 2525 374 146 1280
Fragments arranged by size:
2525 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2678 2691 3590 4187 4974 5242 1239
Size: 393 1046 13 899 597 787 268 1524
Fragments arranged by size:
1524 1046 899 787 597 393 268 13
EcoRI G'AATT_C
Cuts at: 2836 3214 2836
Size: 378 5149
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2588 2681 2708 2588
Size: 93 27 5407
Fragments arranged by size:
5407 93 27
FspI TGC'GCA
Cuts at: 4033 4033
Size: 5527
HaeII r_GCGC'y
Cuts at: 1678 2036 4674 1678
Size: 358 2638 2531
Fragments arranged by size:
2638 2531 358
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2766 2798 4168 4200 4242
Size: 32 135 32 1194 32 1370 32 42
Cuts at: 4242 4274 1373
Size: 32 2626
Fragments arranged by size:
2626 1370 1194 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2335 2640 2871 3117 669
Size: 988 281 397 305 231 246 3079
Fragments arranged by size:
3079 988 397 305 281 246 231
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5527
HpaI GTT'AAC
Cuts at: 3117 3117
Size: 5527
KpnI G_GTAC'C
Cuts at: 2496 2852 2496
Size: 356 5171
MfeI C'AATT_G
Cuts at: 3126 3126
Size: 5527
MluI A'CGCG_T
Cuts at: 5403 5403
Size: 5527
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2499 4641 4825 1305
Size: 1194 2142 184 2007
Fragments arranged by size:
2142 2007 1194 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3705
Fragments arranged by size:
3705 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3485 3844 4003 518
Size: 648 2319 359 159 2042
Fragments arranged by size:
2319 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2368 2527 2635 2737 2943 3620 4768 5013
Size: 762 159 108 102 206 677 1148 245
Cuts at: 5013 1606
Size: 2120
Fragments arranged by size:
2120 1148 762 677 245 206 159 108
102
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4036
Fragments arranged by size:
4036 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5527
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5527
NotI GC'GGCC_GC
Cuts at: 2974 2974
Size: 5527
NspI r_CATG'y
Cuts at: 1132 1229 2885 4430 1132
Size: 97 1656 1545 2229
Fragments arranged by size:
2229 1656 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5527
PciI A'CATG_T
Cuts at: 4426 4426
Size: 5527
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5527
PmeI GTTT'AAAC
Cuts at: 2831 2831
Size: 5527
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3596 3628 5109 5141 3596
Size: 32 1481 32 3982
Fragments arranged by size:
3982 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5527
PsiI TTA'TAA
Cuts at: 3097 3097
Size: 5527
PspOMI G'GGCC_C
Cuts at: 2046 2046
Size: 5527
PstI C_TGCA'G
Cuts at: 830 2067 2879 830
Size: 1237 812 3478
Fragments arranged by size:
3478 1237 812
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5527
PvuII CAG'CTG
Cuts at: 2368 2527 2635 2368
Size: 159 108 5260
Fragments arranged by size:
5260 159 108
SalI G'TCGA_C
Cuts at: 1936 2869 1936
Size: 933 4594
SapI GCTCTTCn'nnn_
Cuts at: 2697 2697
Size: 5527
SbfI CC_TGCA'GG
Cuts at: 2879 2879
Size: 5527
ScaI AGT'ACT
Cuts at: 3775 5487 3775
Size: 1712 3815
SfcI C'TryA_G
Cuts at: 826 1045 2063 2875 4010 4691 4882 826
Size: 219 1018 812 1135 681 191 1471
Fragments arranged by size:
1471 1135 1018 812 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5527
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5527
SmaI CCC'GGG
Cuts at: 1872 2854 1872
Size: 982 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3631 4532 4794 820
Size: 197 926 1688 901 262 1553
Fragments arranged by size:
1688 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3318 493
Size: 2825 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5527
SphI G_CATG'C
Cuts at: 1132 2885 1132
Size: 1753 3774
SspI AAT'ATT
Cuts at: 5 52 3451 5
Size: 47 3399 2081
Fragments arranged by size:
3399 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5527
StyI C'CwwG_G
Cuts at: 513 1065 2004 2028 2353 2421 2966 513
Size: 552 939 24 325 68 545 3074
Fragments arranged by size:
3074 939 552 545 325 68 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2719 2746 3554 3571 3724 3909 4354
Size: 1255 1139 27 808 17 153 185 445
Cuts at: 4354 325
Size: 1498
Fragments arranged by size:
1498 1255 1139 808 445 185 153 27
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3773 5485 96
Size: 274 80 33 51 1443 1796 1712 138
Fragments arranged by size:
1796 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5487 5519 5487
Size: 32 5495
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5527
XbaI T'CTAG_A
Cuts at: 2503 2863 2503
Size: 360 5167
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2035 2035
Size: 5527
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5527
XmaI C'CCGG_G
Cuts at: 1870 2852 1870
Size: 982 4545
XmnI GAAnn'nnTTC
Cuts at: 2691 2703 2821 3656 2691
Size: 12 118 835 4562
Fragments arranged by size:
4562 835 118 12
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3335 276
Size: 53 83 186 936 1801 2468
Fragments arranged by size:
2468 1801 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI