Restriction map for
pFN21AE6655
(Circular) (Six-base) MAPSORT of: pFN21AE6655.seq Check: 1508 from: 1 to: 5464
pFN21AE6655 5464 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2326 2468 2326
Size: 142 5322
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3274 278
Size: 53 83 186 936 1738 2468
Fragments arranged by size:
2468 1738 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2807 1937
Size: 870 4594
Acc65I G'GTAC_C
Cuts at: 2785 2785
Size: 5464
AclI AA'CG_TT
Cuts at: 1563 3591 3964 1563
Size: 2028 373 3063
Fragments arranged by size:
3063 2028 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2204 2459 2696 3527 4911 5179 1632
Size: 572 255 237 831 1384 268 1917
Fragments arranged by size:
1917 1384 831 572 268 255 237
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5267
AflIII A'CryG_T
Cuts at: 1203 2172 4363 5340 1203
Size: 969 2191 977 1327
Fragments arranged by size:
2191 1327 977 969
AgeI A'CCGG_T
Cuts at: 4295 4295
Size: 5464
AhdI GACnn_n'nnGTC
Cuts at: 4193 4193
Size: 5464
AleI CACnn'nnGTG
Cuts at: 1166 2177 1166
Size: 1011 4453
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2561 1184
Size: 1377 4087
AlwNI CAG_nnn'CTG
Cuts at: 2701 3207 4779 2701
Size: 506 1572 3386
Fragments arranged by size:
3386 1572 506
ApaI G_GGCC'C
Cuts at: 2494 2494
Size: 5464
ApaLI G'TGCA_C
Cuts at: 3521 4677 3521
Size: 1156 4308
ApoI r'AATT_y
Cuts at: 1493 2773 2999 3151 5260 1493
Size: 1280 226 152 2109 1697
Fragments arranged by size:
2109 1697 1280 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4018 160
Size: 872 2986 1606
Fragments arranged by size:
2986 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5464
AvaI C'yCGr_G
Cuts at: 1870 1943 2444 2487 2789 1870
Size: 73 501 43 302 4545
Fragments arranged by size:
4545 501 302 73 43
BamHI G'GATC_C
Cuts at: 2643 2794 3161 2643
Size: 151 367 4946
Fragments arranged by size:
4946 367 151
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2259 2785 4240 618
Size: 325 217 627 472 526 1455 1842
Fragments arranged by size:
1842 1455 627 526 472 325 217
BanII G_rGCy'C
Cuts at: 1391 2494 2783 1391
Size: 1103 289 4072
Fragments arranged by size:
4072 1103 289
BbeI G_GCGC'C
Cuts at: 2263 2263
Size: 5464
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4493
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3655 3689 1903
Size: 34 1718 34 3678
Fragments arranged by size:
3678 1718 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3356 4566 3356
Size: 1210 4254
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5464
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2292 2577 2735
Size: 107 122 71 631 605 620 285 158
Cuts at: 2735 4075 136
Size: 1340 1525
Fragments arranged by size:
1525 1340 631 620 605 285 158 122
107 71
BglII A'GATC_T
Cuts at: 2343 5459 2343
Size: 3116 2348
BlpI GC'TnA_GC
Cuts at: 2881 2881
Size: 5464
Bme1580I G_kGCm'C
Cuts at: 1790 2494 3525 4681 1790
Size: 704 1031 1156 2573
Fragments arranged by size:
2573 1156 1031 704
BmgBI CAC'GTC
Cuts at: 1318 2175 1318
Size: 857 4607
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3195 4153 442
Size: 416 53 83 399 24 1778 958 1753
Fragments arranged by size:
1778 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5464
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2368 4124 1239
Size: 1129 1756 2579
Fragments arranged by size:
2579 1756 1129
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2456 3589 4454 4752 2456
Size: 1133 865 298 3168
Fragments arranged by size:
3168 1133 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4127 882
Size: 617 2628 2219
Fragments arranged by size:
2628 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3255 493
Size: 711 2051 2702
Fragments arranged by size:
2702 2051 711
BsaBI GATnn'nnATC
Cuts at: 5458 5458
Size: 5464
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2260 3271 3653 4288
Size: 53 83 186 936 727 1011 382 635
Cuts at: 4288 275
Size: 1451
Fragments arranged by size:
1451 1011 936 727 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3897 4295 4569 4716 5127 3897
Size: 398 274 147 411 4234
Fragments arranged by size:
4234 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2390 2411 2420 2441 1178
Size: 30 165 30 987 21 9 21 4201
Fragments arranged by size:
4201 987 165 30 30 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2126 2345 2525 1186
Size: 940 219 180 4125
Fragments arranged by size:
4125 940 219 180
BseYI C'CCAG_C
Cuts at: 4667 5163 4667
Size: 496 4968
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4891
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2316 2914 3675 4703 1540
Size: 275 186 315 598 761 1028 2301
Fragments arranged by size:
2301 1028 761 598 315 275 186
BsiHKAI G_wGCw'C
Cuts at: 2048 2550 3525 3610 4681 2048
Size: 502 975 85 1071 2831
Fragments arranged by size:
2831 1071 975 502 85
BsmI GAATG_Cn'
Cuts at: 2975 3068 2975
Size: 93 5371
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2048 2494 2550 2783 3525 3610 4681
Size: 399 258 446 56 233 742 85 1071
Cuts at: 4681 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 446 399 258 233 85
56
BspEI T'CCGG_A
Cuts at: 5127 5127
Size: 5464
BspHI T'CATG_A
Cuts at: 3353 5083 3353
Size: 1730 3734
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2326 2468 2640 2819 844
Size: 1482 142 172 179 3489
Fragments arranged by size:
3489 1482 179 172 142
BsrBI CCG'CTC
Cuts at: 1394 1823 2272 3351 1394
Size: 429 449 1079 3507
Fragments arranged by size:
3507 1079 449 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2481 2550 3959 4133 65
Size: 1183 1233 69 1409 174 1396
Fragments arranged by size:
1409 1396 1233 1183 174 69
BsrFI r'CCGG_y
Cuts at: 1700 1810 2534 4108 4295 1700
Size: 110 724 1574 187 2869
Fragments arranged by size:
2869 1574 724 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5464
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5464
BssSI C'ACGA_G
Cuts at: 2021 3524 4536 2021
Size: 1503 1012 2949
Fragments arranged by size:
2949 1503 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5464
BstBI TT'CG_AA
Cuts at: 4268 4268
Size: 5464
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5464
BstYI r'GATC_y
Cuts at: 1972 2343 2643 2794 3161 3546 3563 5015 5101
Size: 371 300 151 367 385 17 1452 86
Cuts at: 5101 5113 5459 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 371 367 346 300 151
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2560 2637 2730 513
Size: 552 939 556 77 93 3247
Fragments arranged by size:
3247 939 556 552 93 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4725
Fragments arranged by size:
4725 683 56
BtsI GCAGTG_nn'
Cuts at: 2980 3774 3794 2980
Size: 794 20 4650
Fragments arranged by size:
4650 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5464
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5429
DraI TTT'AAA
Cuts at: 2768 2768
Size: 5464
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4471 809
Size: 507 3155 1802
Fragments arranged by size:
3155 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2313 2911
Size: 54 1425 50 275 18 177 306 598
Cuts at: 2911 3800 5429 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 598 306 275 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2313 2911 1537
Size: 275 501 598 4090
Fragments arranged by size:
4090 598 501 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3394 1353
Size: 2041 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4051 4425 4571 387
Size: 1064 138 2462 374 146 1280
Fragments arranged by size:
2462 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2204 2368 2459 2696 3527 4124 4911
Size: 393 572 164 91 237 831 597 787
Cuts at: 4911 5179 1239
Size: 268 1524
Fragments arranged by size:
1524 831 787 597 572 393 268 237
164 91
EcoNI CCTnn'n_nnAGG
Cuts at: 2300 2300
Size: 5464
EcoO109I rG'GnC_Cy
Cuts at: 2284 2491 2284
Size: 207 5257
EcoRI G'AATT_C
Cuts at: 2773 3151 2773
Size: 378 5086
FspI TGC'GCA
Cuts at: 2252 3970 2252
Size: 1718 3746
FspAI rTGC'GCAy
Cuts at: 2252 2252
Size: 5464
HaeII r_GCGC'y
Cuts at: 1678 2263 2356 4611 1678
Size: 585 93 2255 2531
Fragments arranged by size:
2531 2255 585 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4105 4137 4179 4211 1373
Size: 32 135 32 2533 32 42 32 2626
Fragments arranged by size:
2626 2533 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2808 3054 669
Size: 988 281 870 246 3079
Fragments arranged by size:
3079 988 870 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5464
HpaI GTT'AAC
Cuts at: 3054 3054
Size: 5464
KasI G'GCGC_C
Cuts at: 2259 2259
Size: 5464
KpnI G_GTAC'C
Cuts at: 2789 2789
Size: 5464
MfeI C'AATT_G
Cuts at: 3063 3063
Size: 5464
MluI A'CGCG_T
Cuts at: 5340 5340
Size: 5464
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4578 4762 1305
Size: 3273 184 2007
Fragments arranged by size:
3273 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 3465
Fragments arranged by size:
3465 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 3422 3781 3940 518
Size: 648 1011 1245 359 159 2042
Fragments arranged by size:
2042 1245 1011 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2880 3557 4705 4950 1606
Size: 1274 677 1148 245 2120
Fragments arranged by size:
2120 1274 1148 677 245
NaeI GCC'GGC
Cuts at: 2536 2536
Size: 5464
NarI GG'CG_CC
Cuts at: 2260 2260
Size: 5464
NcoI C'CATG_G
Cuts at: 513 1065 2004 2730 513
Size: 552 939 726 3247
Fragments arranged by size:
3247 939 726 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5464
NgoMIV G'CCGG_C
Cuts at: 2534 2534
Size: 5464
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5464
NotI GC'GGCC_GC
Cuts at: 2911 2911
Size: 5464
NspI r_CATG'y
Cuts at: 1132 1229 2822 4367 1132
Size: 97 1593 1545 2229
Fragments arranged by size:
2229 1593 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2749 1348
Size: 1401 4063
PciI A'CATG_T
Cuts at: 4363 4363
Size: 5464
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5464
PfoI T'CCnGG_A
Cuts at: 2214 2214
Size: 5464
PmeI GTTT'AAAC
Cuts at: 2768 2768
Size: 5464
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3533 3565 5046 5078 3533
Size: 32 1481 32 3919
Fragments arranged by size:
3919 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5464
PsiI TTA'TAA
Cuts at: 3034 3034
Size: 5464
PspOMI G'GGCC_C
Cuts at: 2490 2490
Size: 5464
PstI C_TGCA'G
Cuts at: 830 2816 830
Size: 1986 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5464
SalI G'TCGA_C
Cuts at: 1936 2806 1936
Size: 870 4594
SbfI CC_TGCA'GG
Cuts at: 2816 2816
Size: 5464
ScaI AGT'ACT
Cuts at: 3712 5424 3712
Size: 1712 3752
SfcI C'TryA_G
Cuts at: 826 1045 2812 3947 4628 4819 826
Size: 219 1767 1135 681 191 1471
Fragments arranged by size:
1767 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2292 2292
Size: 5464
SfoI GGC'GCC
Cuts at: 2261 2261
Size: 5464
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5464
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5464
SmaI CCC'GGG
Cuts at: 1872 2446 2489 2791 1872
Size: 574 43 302 4545
Fragments arranged by size:
4545 574 302 43
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2471 3568 4469 4731 820
Size: 197 926 528 1097 901 262 1553
Fragments arranged by size:
1553 1097 926 901 528 262 197
SnaBI TAC'GTA
Cuts at: 493 3255 493
Size: 2762 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5464
SphI G_CATG'C
Cuts at: 1132 2822 1132
Size: 1690 3774
SrfI GCCC'GGGC
Cuts at: 2489 2489
Size: 5464
SspI AAT'ATT
Cuts at: 5 52 3388 5
Size: 47 3336 2081
Fragments arranged by size:
3336 2081 47
StuI AGG'CCT
Cuts at: 1517 2295 2436 1517
Size: 778 141 4545
Fragments arranged by size:
4545 778 141
StyI C'CwwG_G
Cuts at: 513 1065 2004 2130 2730 2760 2903 513
Size: 552 939 126 600 30 143 3074
Fragments arranged by size:
3074 939 600 552 143 126 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2123 2693 3491 3508 3661 3846 4291
Size: 1255 543 570 798 17 153 185 445
Cuts at: 4291 325
Size: 1498
Fragments arranged by size:
1498 1255 798 570 543 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3710 5422 96
Size: 274 80 33 51 1443 1733 1712 138
Fragments arranged by size:
1733 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2219 2251 5424 5456 2219
Size: 32 3173 32 2227
Fragments arranged by size:
3173 2227 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5464
XbaI T'CTAG_A
Cuts at: 2800 2800
Size: 5464
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2698 2737 2698
Size: 39 5425
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5464
XmaI C'CCGG_G
Cuts at: 1870 2444 2487 2789 1870
Size: 574 43 302 4545
Fragments arranged by size:
4545 574 302 43
XmnI GAAnn'nnTTC
Cuts at: 2066 3593 2066
Size: 1527 3937
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3272 276
Size: 53 83 186 936 1738 2468
Fragments arranged by size:
2468 1738 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI NruI NsiI PacI PmlI PpuMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SwaI