Restriction map for
pFN21AE6652
(Circular) (Six-base) MAPSORT of: pFN21AE6652.seq Check: 8663 from: 1 to: 5440
pFN21AE6652 5440 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2673 3250 278
Size: 53 83 186 936 1137 577 2468
Fragments arranged by size:
2468 1137 936 577 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2418 2783 1937
Size: 481 365 4594
Fragments arranged by size:
4594 481 365
Acc65I G'GTAC_C
Cuts at: 2761 2761
Size: 5440
AclI AA'CG_TT
Cuts at: 1563 3567 3940 1563
Size: 2004 373 3063
Fragments arranged by size:
3063 2004 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2462 2505 3503 4887 5155 1632
Size: 830 43 998 1384 268 1917
Fragments arranged by size:
1917 1384 998 830 268 43
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5243
AflIII A'CryG_T
Cuts at: 1203 4339 5316 1203
Size: 3136 977 1327
Fragments arranged by size:
3136 1327 977
AgeI A'CCGG_T
Cuts at: 4271 4271
Size: 5440
AhdI GACnn_n'nnGTC
Cuts at: 4169 4169
Size: 5440
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5440
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5440
AlwNI CAG_nnn'CTG
Cuts at: 2599 3183 4755 2599
Size: 584 1572 3284
Fragments arranged by size:
3284 1572 584
ApaLI G'TGCA_C
Cuts at: 3497 4653 3497
Size: 1156 4284
ApoI r'AATT_y
Cuts at: 1493 2749 2975 3127 5236 1493
Size: 1256 226 152 2109 1697
Fragments arranged by size:
2109 1697 1256 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3994 160
Size: 872 2962 1606
Fragments arranged by size:
2962 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5440
AvaI C'yCGr_G
Cuts at: 1870 1943 2765 1870
Size: 73 822 4545
Fragments arranged by size:
4545 822 73
BamHI G'GATC_C
Cuts at: 2770 3137 2770
Size: 367 5073
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2761 4216 618
Size: 325 217 627 974 1455 1842
Fragments arranged by size:
1842 1455 974 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2759 1391
Size: 1368 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4469
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3631 3665 1903
Size: 34 1694 34 3678
Fragments arranged by size:
3678 1694 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3332 4542 3332
Size: 1210 4230
BclI T'GATC_A
Cuts at: 1548 2472 1548
Size: 924 4516
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4051 136
Size: 107 122 71 631 605 2379 1525
Fragments arranged by size:
2379 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5435 5435
Size: 5440
BlpI GC'TnA_GC
Cuts at: 2857 2857
Size: 5440
Bme1580I G_kGCm'C
Cuts at: 1790 3501 4657 1790
Size: 1711 1156 2573
Fragments arranged by size:
2573 1711 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5440
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3171 4129 442
Size: 416 53 83 399 24 1754 958 1753
Fragments arranged by size:
1754 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5440
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2075 2298 4100 1239
Size: 836 223 1802 2579
Fragments arranged by size:
2579 1802 836 223
Bpu10I CC'TnA_GC
Cuts at: 2642 2642
Size: 5440
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2589 3565 4430 4728 2589
Size: 976 865 298 3301
Fragments arranged by size:
3301 976 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4103 882
Size: 617 2604 2219
Fragments arranged by size:
2604 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2548 2629 3231 493
Size: 711 1344 81 602 2702
Fragments arranged by size:
2702 1344 711 602 81
BsaBI GATnn'nnATC
Cuts at: 5434 5434
Size: 5440
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2325 2670 3247 3629
Size: 53 83 186 936 792 345 577 382
Cuts at: 3629 4264 275
Size: 635 1451
Fragments arranged by size:
1451 936 792 635 577 382 345 186
83 53
BsaWI w'CCGG_w
Cuts at: 3873 4271 4545 4692 5103 3873
Size: 398 274 147 411 4210
Fragments arranged by size:
4210 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2201 2231 1178
Size: 30 165 30 798 30 4387
Fragments arranged by size:
4387 798 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2720 1186
Size: 1534 3906
BseYI C'CCAG_C
Cuts at: 2130 4643 5139 2130
Size: 2513 496 2431
Fragments arranged by size:
2513 2431 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2058 2651 1163
Size: 573 322 593 3952
Fragments arranged by size:
3952 593 573 322
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2890 3651 4679 1540
Size: 275 186 889 761 1028 2301
Fragments arranged by size:
2301 1028 889 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2519 3501 3586 4657 2519
Size: 982 85 1071 3302
Fragments arranged by size:
3302 1071 982 85
BsmI GAATG_Cn'
Cuts at: 2200 2951 3044 2200
Size: 751 93 4596
Fragments arranged by size:
4596 751 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2519 2759 3501 3586 4657 1391
Size: 399 729 240 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 729 399 240 85
BspEI T'CCGG_A
Cuts at: 5103 5103
Size: 5440
BspHI T'CATG_A
Cuts at: 3329 5059 3329
Size: 1730 3710
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2795 844
Size: 1951 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3327 1394
Size: 429 1504 3507
Fragments arranged by size:
3507 1504 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2636 3935 4109 65
Size: 1183 1388 1299 174 1396
Fragments arranged by size:
1396 1388 1299 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4084 4271 1700
Size: 110 2274 187 2869
Fragments arranged by size:
2869 2274 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5440
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5440
BssSI C'ACGA_G
Cuts at: 3500 4512 3500
Size: 1012 4428
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2638 794
Size: 1844 3596
BstBI TT'CG_AA
Cuts at: 4244 4244
Size: 5440
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5440
BstXI CCAn_nnnn'nTGG
Cuts at: 2276 2276
Size: 5440
BstYI r'GATC_y
Cuts at: 1972 2437 2770 3137 3522 3539 4991 5077 5089
Size: 465 333 367 385 17 1452 86 12
Cuts at: 5089 5435 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 465 385 367 346 333 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2552 2614 513
Size: 552 939 548 62 3339
Fragments arranged by size:
3339 939 552 548 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4701
Fragments arranged by size:
4701 683 56
BtsI GCAGTG_nn'
Cuts at: 2956 3750 3770 2956
Size: 794 20 4626
Fragments arranged by size:
4626 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5440
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5405
DraI TTT'AAA
Cuts at: 2112 2744 2112
Size: 632 4808
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4447 809
Size: 507 3131 1802
Fragments arranged by size:
3131 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2887 3776 5405
Size: 54 1425 50 275 18 1057 889 1629
Cuts at: 5405 8
Size: 43
Fragments arranged by size:
1629 1425 1057 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2887 1537
Size: 275 1075 4090
Fragments arranged by size:
4090 1075 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2524 3370 1353
Size: 1171 846 3423
Fragments arranged by size:
3423 1171 846
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4027 4401 4547 387
Size: 1064 138 2438 374 146 1280
Fragments arranged by size:
2438 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2298 2462 2505 3503 4100 4887
Size: 393 443 223 164 43 998 597 787
Cuts at: 4887 5155 1239
Size: 268 1524
Fragments arranged by size:
1524 998 787 597 443 393 268 223
164 43
EcoO109I rG'GnC_Cy
Cuts at: 2596 2596
Size: 5440
EcoRI G'AATT_C
Cuts at: 2749 3127 2749
Size: 378 5062
FspI TGC'GCA
Cuts at: 3946 3946
Size: 5440
HaeII r_GCGC'y
Cuts at: 1678 4587 1678
Size: 2909 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4081 4113 4155 4187 1373
Size: 32 135 32 2509 32 42 32 2626
Fragments arranged by size:
2626 2509 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2784 3030 669
Size: 988 281 846 246 3079
Fragments arranged by size:
3079 988 846 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5440
HpaI GTT'AAC
Cuts at: 3030 3030
Size: 5440
KpnI G_GTAC'C
Cuts at: 2765 2765
Size: 5440
MfeI C'AATT_G
Cuts at: 3039 3039
Size: 5440
MluI A'CGCG_T
Cuts at: 5316 5316
Size: 5440
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4554 4738 1305
Size: 3249 184 2007
Fragments arranged by size:
3249 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3618
Fragments arranged by size:
3618 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2210 3398 3757 3916 518
Size: 648 1044 1188 359 159 2042
Fragments arranged by size:
2042 1188 1044 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2394 2663 2856 3533 4681 4926 1606
Size: 788 269 193 677 1148 245 2120
Fragments arranged by size:
2120 1148 788 677 269 245 193
NcoI C'CATG_G
Cuts at: 513 1065 2004 2552 513
Size: 552 939 548 3401
Fragments arranged by size:
3401 939 552 548
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5440
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5440
NotI GC'GGCC_GC
Cuts at: 2887 2887
Size: 5440
NspI r_CATG'y
Cuts at: 1132 1229 2252 2798 4343 1132
Size: 97 1023 546 1545 2229
Fragments arranged by size:
2229 1545 1023 546 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5440
PciI A'CATG_T
Cuts at: 4339 4339
Size: 5440
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5440
PmeI GTTT'AAAC
Cuts at: 2744 2744
Size: 5440
PmlI CAC'GTG
Cuts at: 2548 2629 2548
Size: 81 5359
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3509 3541 5022 5054 3509
Size: 32 1481 32 3895
Fragments arranged by size:
3895 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5440
PsiI TTA'TAA
Cuts at: 3010 3010
Size: 5440
PstI C_TGCA'G
Cuts at: 830 2355 2792 830
Size: 1525 437 3478
Fragments arranged by size:
3478 1525 437
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5440
SalI G'TCGA_C
Cuts at: 1936 2782 1936
Size: 846 4594
SapI GCTCTTCn'nnn_
Cuts at: 2524 2524
Size: 5440
SbfI CC_TGCA'GG
Cuts at: 2792 2792
Size: 5440
ScaI AGT'ACT
Cuts at: 3688 5400 3688
Size: 1712 3728
SfcI C'TryA_G
Cuts at: 826 1045 2351 2788 3923 4604 4795 826
Size: 219 1306 437 1135 681 191 1471
Fragments arranged by size:
1471 1306 1135 681 437 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5440
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5440
SmaI CCC'GGG
Cuts at: 1872 2767 1872
Size: 895 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2604 3544 4445 4707 820
Size: 197 926 661 940 901 262 1553
Fragments arranged by size:
1553 940 926 901 661 262 197
SnaBI TAC'GTA
Cuts at: 493 3231 493
Size: 2738 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5440
SphI G_CATG'C
Cuts at: 1132 2798 1132
Size: 1666 3774
SspI AAT'ATT
Cuts at: 5 52 3364 5
Size: 47 3312 2081
Fragments arranged by size:
3312 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5440
StyI C'CwwG_G
Cuts at: 513 1065 2004 2187 2552 2879 513
Size: 552 939 183 365 327 3074
Fragments arranged by size:
3074 939 552 365 327 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2238 2517 3467 3484 3637 3822 4267
Size: 1255 658 279 950 17 153 185 445
Cuts at: 4267 325
Size: 1498
Fragments arranged by size:
1498 1255 950 658 445 279 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2295 3686 5398
Size: 274 80 33 51 1443 318 1391 1712
Cuts at: 5398 96
Size: 138
Fragments arranged by size:
1712 1443 1391 318 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2201 2233 5400 5432 2201
Size: 32 3167 32 2209
Fragments arranged by size:
3167 2209 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5440
XbaI T'CTAG_A
Cuts at: 2776 2776
Size: 5440
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5440
XmaI C'CCGG_G
Cuts at: 1870 2765 1870
Size: 895 4545
XmnI GAAnn'nnTTC
Cuts at: 3569 3569
Size: 5440
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2671 3248 276
Size: 53 83 186 936 1137 577 2468
Fragments arranged by size:
2468 1137 936 577 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI