Restriction map for
pFN21AE6646
(Circular) (Six-base) MAPSORT of: pFN21AE6646.seq Check: 8529 from: 1 to: 5383
pFN21AE6646 5383 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1999 2354 1999
Size: 355 5028
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3193 278
Size: 53 83 186 936 1657 2468
Fragments arranged by size:
2468 1657 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2726 1937
Size: 789 4594
Acc65I G'GTAC_C
Cuts at: 2619 2704 2619
Size: 85 5298
AclI AA'CG_TT
Cuts at: 1563 3510 3883 1563
Size: 1947 373 3063
Fragments arranged by size:
3063 1947 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2684 3446 4830 5098 1632
Size: 1052 762 1384 268 1917
Fragments arranged by size:
1917 1384 1052 762 268
AfeI AGC'GCT
Cuts at: 2596 2596
Size: 5383
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5186
AflIII A'CryG_T
Cuts at: 1203 4282 5259 1203
Size: 3079 977 1327
Fragments arranged by size:
3079 1327 977
AgeI A'CCGG_T
Cuts at: 4214 4214
Size: 5383
AhdI GACnn_n'nnGTC
Cuts at: 4112 4112
Size: 5383
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5383
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5383
AlwNI CAG_nnn'CTG
Cuts at: 3126 4698 3126
Size: 1572 3811
ApaLI G'TGCA_C
Cuts at: 3440 4596 3440
Size: 1156 4227
ApoI r'AATT_y
Cuts at: 1493 2443 2692 2918 3070 5179 1493
Size: 950 249 226 152 2109 1697
Fragments arranged by size:
2109 1697 950 249 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3937 160
Size: 872 2905 1606
Fragments arranged by size:
2905 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5383
AvaI C'yCGr_G
Cuts at: 1870 1943 2708 1870
Size: 73 765 4545
Fragments arranged by size:
4545 765 73
BamHI G'GATC_C
Cuts at: 2713 3080 2713
Size: 367 5016
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2318 2619 2704 4159 618
Size: 325 217 627 531 301 85 1455 1842
Fragments arranged by size:
1842 1455 627 531 325 301 217 85
BanII G_rGCy'C
Cuts at: 1391 2702 1391
Size: 1311 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4412
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3574 3608 1903
Size: 34 50 34 1553 34 3678
Fragments arranged by size:
3678 1553 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3275 4485 3275
Size: 1210 4173
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5383
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3994 136
Size: 107 122 71 631 605 2322 1525
Fragments arranged by size:
2322 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5378 5378
Size: 5383
BlpI GC'TnA_GC
Cuts at: 2800 2800
Size: 5383
Bme1580I G_kGCm'C
Cuts at: 1790 3444 4600 1790
Size: 1654 1156 2573
Fragments arranged by size:
2573 1654 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5383
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2118 2569 3114
Size: 416 53 83 399 24 701 451 545
Cuts at: 3114 4072 442
Size: 958 1753
Fragments arranged by size:
1753 958 701 545 451 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5383
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2568 2568
Size: 5383
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2361 4043 1239
Size: 1122 1682 2579
Fragments arranged by size:
2579 1682 1122
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3508 4373 4671 3508
Size: 865 298 4220
Fragments arranged by size:
4220 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4046 882
Size: 617 2547 2219
Fragments arranged by size:
2547 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3174 493
Size: 711 1970 2702
Fragments arranged by size:
2702 1970 711
BsaBI GATnn'nnATC
Cuts at: 5377 5377
Size: 5383
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3190 3572 4207 275
Size: 53 83 186 936 1657 382 635 1451
Fragments arranged by size:
1657 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3816 4214 4488 4635 5046 3816
Size: 398 274 147 411 4153
Fragments arranged by size:
4153 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5158
Fragments arranged by size:
5158 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5383
BseYI C'CCAG_C
Cuts at: 2213 4586 5082 2213
Size: 2373 496 2514
Fragments arranged by size:
2514 2373 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2382 1163
Size: 573 646 4164
Fragments arranged by size:
4164 646 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2833 3594 4622 1540
Size: 275 186 832 761 1028 2301
Fragments arranged by size:
2301 1028 832 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2493 3444 3529 4600 2493
Size: 951 85 1071 3276
Fragments arranged by size:
3276 1071 951 85
BsmI GAATG_Cn'
Cuts at: 2231 2674 2894 2987 2231
Size: 443 220 93 4627
Fragments arranged by size:
4627 443 220 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2493 2702 3444 3529 4600 1391
Size: 399 703 209 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 703 399 209 85
BspEI T'CCGG_A
Cuts at: 5046 5046
Size: 5383
BspHI T'CATG_A
Cuts at: 3272 5002 3272
Size: 1730 3653
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 1999 2354 2738 844
Size: 1155 355 384 3489
Fragments arranged by size:
3489 1155 384 355
BsrBI CCG'CTC
Cuts at: 1394 1823 3270 1394
Size: 429 1447 3507
Fragments arranged by size:
3507 1447 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3878 4052 65
Size: 1183 2630 174 1396
Fragments arranged by size:
2630 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4027 4214 1700
Size: 110 2217 187 2869
Fragments arranged by size:
2869 2217 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5383
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5383
BssSI C'ACGA_G
Cuts at: 3443 4455 3443
Size: 1012 4371
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5383
BstBI TT'CG_AA
Cuts at: 4187 4187
Size: 5383
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5383
BstYI r'GATC_y
Cuts at: 1972 2456 2713 3080 3465 3482 4934 5020 5032
Size: 484 257 367 385 17 1452 86 12
Cuts at: 5032 5378 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 484 385 367 346 257 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4831
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4644
Fragments arranged by size:
4644 683 56
BtsI GCAGTG_nn'
Cuts at: 2236 2344 2899 3693 3713 2236
Size: 108 555 794 20 3906
Fragments arranged by size:
3906 794 555 108 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5383
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5348
DraI TTT'AAA
Cuts at: 2687 2687
Size: 5383
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4390 809
Size: 507 3074 1802
Fragments arranged by size:
3074 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2103 2419 2830
Size: 54 1425 50 275 18 273 316 411
Cuts at: 2830 3719 5348 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 411 316 275 273 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2830 1537
Size: 275 1018 4090
Fragments arranged by size:
4090 1018 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2417 3313 1353
Size: 1064 896 3423
Fragments arranged by size:
3423 1064 896
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3970 4344 4490 387
Size: 1064 138 2381 374 146 1280
Fragments arranged by size:
2381 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2361 2684 3446 4043 4830 5098 1239
Size: 393 729 323 762 597 787 268 1524
Fragments arranged by size:
1524 787 762 729 597 393 323 268
EcoRI G'AATT_C
Cuts at: 2692 3070 2692
Size: 378 5005
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2428 2428
Size: 5383
FspI TGC'GCA
Cuts at: 3889 3889
Size: 5383
HaeII r_GCGC'y
Cuts at: 1678 2598 4530 1678
Size: 920 1932 2531
Fragments arranged by size:
2531 1932 920
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2448 2480 4024 4056 4098
Size: 32 135 32 876 32 1544 32 42
Cuts at: 4098 4130 1373
Size: 32 2626
Fragments arranged by size:
2626 1544 876 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2284 2631 2727 2973 669
Size: 988 281 346 347 96 246 3079
Fragments arranged by size:
3079 988 347 346 281 246 96
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5383
HpaI GTT'AAC
Cuts at: 2973 2973
Size: 5383
KpnI G_GTAC'C
Cuts at: 2623 2708 2623
Size: 85 5298
MfeI C'AATT_G
Cuts at: 2982 2982
Size: 5383
MluI A'CGCG_T
Cuts at: 5259 5259
Size: 5383
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4497 4681 1305
Size: 3192 184 2007
Fragments arranged by size:
3192 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2105 2421 10
Size: 54 1425 343 273 316 2972
Fragments arranged by size:
2972 1425 343 316 273 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3341 3700 3859 518
Size: 648 843 1332 359 159 2042
Fragments arranged by size:
2042 1332 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2799 3476 4624 4869 1606
Size: 1193 677 1148 245 2120
Fragments arranged by size:
2120 1193 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4831
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5383
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5383
NotI GC'GGCC_GC
Cuts at: 2830 2830
Size: 5383
NspI r_CATG'y
Cuts at: 1132 1229 2741 4286 1132
Size: 97 1512 1545 2229
Fragments arranged by size:
2229 1545 1512 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5383
PciI A'CATG_T
Cuts at: 4282 4282
Size: 5383
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2133 1108
Size: 1025 4358
PmeI GTTT'AAAC
Cuts at: 2687 2687
Size: 5383
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3452 3484 4965 4997 3452
Size: 32 1481 32 3838
Fragments arranged by size:
3838 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5383
PsiI TTA'TAA
Cuts at: 2953 2953
Size: 5383
PstI C_TGCA'G
Cuts at: 830 2735 830
Size: 1905 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5383
SalI G'TCGA_C
Cuts at: 1936 2725 1936
Size: 789 4594
SapI GCTCTTCn'nnn_
Cuts at: 2417 2417
Size: 5383
SbfI CC_TGCA'GG
Cuts at: 2735 2735
Size: 5383
ScaI AGT'ACT
Cuts at: 3631 5343 3631
Size: 1712 3671
SfcI C'TryA_G
Cuts at: 826 1045 2731 3866 4547 4738 826
Size: 219 1686 1135 681 191 1471
Fragments arranged by size:
1686 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5383
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5383
SmaI CCC'GGG
Cuts at: 1872 2710 1872
Size: 838 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3487 4388 4650 820
Size: 197 926 1544 901 262 1553
Fragments arranged by size:
1553 1544 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3174 493
Size: 2681 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5383
SphI G_CATG'C
Cuts at: 1132 2741 1132
Size: 1609 3774
SspI AAT'ATT
Cuts at: 5 52 2180 3307 5
Size: 47 2128 1127 2081
Fragments arranged by size:
2128 2081 1127 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5383
StyI C'CwwG_G
Cuts at: 513 1065 2822 513
Size: 552 1757 3074
Fragments arranged by size:
3074 1757 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3410 3427 3580 3765 4210 325
Size: 1255 1830 17 153 185 445 1498
Fragments arranged by size:
1830 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2567 3629 5341
Size: 274 80 33 51 1443 590 1062 1712
Cuts at: 5341 96
Size: 138
Fragments arranged by size:
1712 1443 1062 590 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5343 5375 5343
Size: 32 5351
Tth111I GACn'n_nGTC
Cuts at: 1316 2635 1316
Size: 1319 4064
XbaI T'CTAG_A
Cuts at: 2719 2719
Size: 5383
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2385 2385
Size: 5383
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5383
XmaI C'CCGG_G
Cuts at: 1870 2708 1870
Size: 838 4545
XmnI GAAnn'nnTTC
Cuts at: 2411 3512 2411
Size: 1101 4282
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3191 276
Size: 53 83 186 936 1657 2468
Fragments arranged by size:
2468 1657 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI