Restriction map for
pFN21AE6645
(Circular) (Six-base) MAPSORT of: pFN21AE6645.seq Check: 6824 from: 1 to: 5560
pFN21AE6645 5560 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3370 278
Size: 53 83 186 936 1834 2468
Fragments arranged by size:
2468 1834 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2903 1937
Size: 966 4594
Acc65I G'GTAC_C
Cuts at: 2881 2881
Size: 5560
AclI AA'CG_TT
Cuts at: 1563 3687 4060 1563
Size: 2124 373 3063
Fragments arranged by size:
3063 2124 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2169 2197 2355 2421 2840 3623 5007 5275
Size: 537 28 158 66 419 783 1384 268
Cuts at: 5275 1632
Size: 1917
Fragments arranged by size:
1917 1384 783 537 419 268 158 66
28
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5363
AflIII A'CryG_T
Cuts at: 1203 4459 5436 1203
Size: 3256 977 1327
Fragments arranged by size:
3256 1327 977
AgeI A'CCGG_T
Cuts at: 4391 4391
Size: 5560
AhdI GACnn_n'nnGTC
Cuts at: 4289 4289
Size: 5560
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5560
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5560
AlwNI CAG_nnn'CTG
Cuts at: 2269 3303 4875 2269
Size: 1034 1572 2954
Fragments arranged by size:
2954 1572 1034
ApaLI G'TGCA_C
Cuts at: 3617 4773 3617
Size: 1156 4404
ApoI r'AATT_y
Cuts at: 1493 2297 2587 2869 3095 3247 5356 1493
Size: 804 290 282 226 152 2109 1697
Fragments arranged by size:
2109 1697 804 290 282 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4114 160
Size: 872 3082 1606
Fragments arranged by size:
3082 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5560
AvaI C'yCGr_G
Cuts at: 1870 1943 2885 1870
Size: 73 942 4545
Fragments arranged by size:
4545 942 73
BamHI G'GATC_C
Cuts at: 2664 2890 3257 2664
Size: 226 367 4967
Fragments arranged by size:
4967 367 226
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2881 4336 618
Size: 325 217 627 1094 1455 1842
Fragments arranged by size:
1842 1455 1094 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2052 2127 2879 1391
Size: 661 75 752 4072
Fragments arranged by size:
4072 752 661 75
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4589
BbvCI CC'TCA_GC
Cuts at: 2642 2642
Size: 5560
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3751 3785 1903
Size: 34 1814 34 3678
Fragments arranged by size:
3678 1814 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3452 4662 3452
Size: 1210 4350
BclI T'GATC_A
Cuts at: 1548 2181 1548
Size: 633 4927
BfrBI ATG'CAT
Cuts at: 2254 2254
Size: 5560
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4171 136
Size: 107 122 71 631 605 2499 1525
Fragments arranged by size:
2499 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2742 5555 2742
Size: 2813 2747
BlpI GC'TnA_GC
Cuts at: 2977 2977
Size: 5560
Bme1580I G_kGCm'C
Cuts at: 1790 3621 4777 1790
Size: 1831 1156 2573
Fragments arranged by size:
2573 1831 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5560
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3291 4249 442
Size: 416 53 83 399 24 1874 958 1753
Fragments arranged by size:
1874 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5560
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2149 2291 2613 4220 1239
Size: 910 142 322 1607 2579
Fragments arranged by size:
2579 1607 910 322 142
Bpu10I CC'TnA_GC
Cuts at: 2642 2642
Size: 5560
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3685 4550 4848 3685
Size: 865 298 4397
Fragments arranged by size:
4397 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4223 882
Size: 617 2724 2219
Fragments arranged by size:
2724 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3351 493
Size: 711 2147 2702
Fragments arranged by size:
2702 2147 711
BsaBI GATnn'nnATC
Cuts at: 2238 5554 2238
Size: 3316 2244
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3367 3749 4384 275
Size: 53 83 186 936 1834 382 635 1451
Fragments arranged by size:
1834 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3993 4391 4665 4812 5223 3993
Size: 398 274 147 411 4330
Fragments arranged by size:
4330 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2424 2454 1178
Size: 30 165 30 1021 30 4284
Fragments arranged by size:
4284 1021 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2052 1186
Size: 866 4694
BseYI C'CCAG_C
Cuts at: 4763 5259 4763
Size: 496 5064
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2359 1163
Size: 573 623 4364
Fragments arranged by size:
4364 623 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3010 3771 4799 1540
Size: 275 186 1009 761 1028 2301
Fragments arranged by size:
2301 1028 1009 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3621 3706 4777 3621
Size: 85 1071 4404
Fragments arranged by size:
4404 1071 85
BsmI GAATG_Cn'
Cuts at: 3071 3164 3071
Size: 93 5467
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2127 2879 3621 3706 4777 1391
Size: 399 262 75 752 742 85 1071 2174
Fragments arranged by size:
2174 1071 752 742 399 262 85 75
BspEI T'CCGG_A
Cuts at: 5223 5223
Size: 5560
BspHI T'CATG_A
Cuts at: 3449 5179 3449
Size: 1730 3830
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2623 2915 844
Size: 1779 292 3489
Fragments arranged by size:
3489 1779 292
BsrBI CCG'CTC
Cuts at: 1394 1823 2046 3447 1394
Size: 429 223 1401 3507
Fragments arranged by size:
3507 1401 429 223
BsrDI GCAATG_nn'
Cuts at: 65 1248 2844 4055 4229 65
Size: 1183 1596 1211 174 1396
Fragments arranged by size:
1596 1396 1211 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4204 4391 1700
Size: 110 2394 187 2869
Fragments arranged by size:
2869 2394 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5560
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5560
BssSI C'ACGA_G
Cuts at: 3620 4632 3620
Size: 1012 4548
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5560
BstBI TT'CG_AA
Cuts at: 4364 4364
Size: 5560
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5560
BstYI r'GATC_y
Cuts at: 1972 2599 2664 2742 2890 3257 3642 3659 5111
Size: 627 65 78 148 367 385 17 1452
Cuts at: 5111 5197 5209 5555 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 627 385 367 346 148 86
78 65 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5008
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4821
Fragments arranged by size:
4821 683 56
BtsI GCAGTG_nn'
Cuts at: 2347 3076 3870 3890 2347
Size: 729 794 20 4017
Fragments arranged by size:
4017 794 729 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5560
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5525
DraI TTT'AAA
Cuts at: 2864 2864
Size: 5560
DraIII CAC_nnn'GTG
Cuts at: 2337 2337
Size: 5560
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4567 809
Size: 507 3251 1802
Fragments arranged by size:
3251 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2449 2749 3007
Size: 54 1425 50 275 18 619 300 258
Cuts at: 3007 3896 5525 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 619 300 275 258 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3007 1537
Size: 275 1195 4090
Fragments arranged by size:
4090 1195 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2059 2719 3490 1353
Size: 706 660 771 3423
Fragments arranged by size:
3423 771 706 660
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4147 4521 4667 387
Size: 1064 138 2558 374 146 1280
Fragments arranged by size:
2558 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2149 2169 2197 2291 2355 2421 2613
Size: 393 517 20 28 94 64 66 192
Cuts at: 2613 2840 3623 4220 5007 5275 1239
Size: 227 783 597 787 268 1524
Fragments arranged by size:
1524 787 783 597 517 393 268 227
192 94 66 64 28 20
EcoNI CCTnn'n_nnAGG
Cuts at: 2151 2855 2151
Size: 704 4856
EcoO109I rG'GnC_Cy
Cuts at: 2266 2266
Size: 5560
EcoRI G'AATT_C
Cuts at: 2587 2869 3247 2587
Size: 282 378 4900
Fragments arranged by size:
4900 378 282
EcoRV GAT'ATC
Cuts at: 2693 2693
Size: 5560
FspI TGC'GCA
Cuts at: 4066 4066
Size: 5560
HaeII r_GCGC'y
Cuts at: 1678 4707 1678
Size: 3029 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2656 2688 4201 4233 4275
Size: 32 135 32 1084 32 1513 32 42
Cuts at: 4275 4307 1373
Size: 32 2626
Fragments arranged by size:
2626 1513 1084 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2904 3150 669
Size: 988 281 966 246 3079
Fragments arranged by size:
3079 988 966 281 246
HindIII A'AGCT_T
Cuts at: 748 2482 748
Size: 1734 3826
HpaI GTT'AAC
Cuts at: 3150 3150
Size: 5560
KpnI G_GTAC'C
Cuts at: 2885 2885
Size: 5560
MfeI C'AATT_G
Cuts at: 3159 3159
Size: 5560
MluI A'CGCG_T
Cuts at: 5436 5436
Size: 5560
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4674 4858 1305
Size: 3369 184 2007
Fragments arranged by size:
3369 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2451 2751 10
Size: 54 1425 343 619 300 2819
Fragments arranged by size:
2819 1425 619 343 300 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2460 3518 3877 4036 518
Size: 648 1294 1058 359 159 2042
Fragments arranged by size:
2042 1294 1058 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2976 3653 4801 5046 1606
Size: 1370 677 1148 245 2120
Fragments arranged by size:
2120 1370 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5008
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5560
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5560
NotI GC'GGCC_GC
Cuts at: 3007 3007
Size: 5560
NsiI A_TGCA'T
Cuts at: 2256 2256
Size: 5560
NspI r_CATG'y
Cuts at: 1132 1229 2918 4463 1132
Size: 97 1689 1545 2229
Fragments arranged by size:
2229 1689 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5560
PciI A'CATG_T
Cuts at: 4459 4459
Size: 5560
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2269 1108
Size: 1161 4399
PfoI T'CCnGG_A
Cuts at: 2136 2268 2136
Size: 132 5428
PmeI GTTT'AAAC
Cuts at: 2864 2864
Size: 5560
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3629 3661 5142 5174 3629
Size: 32 1481 32 4015
Fragments arranged by size:
4015 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2266 2266
Size: 5560
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5560
PsiI TTA'TAA
Cuts at: 2573 3130 2573
Size: 557 5003
PstI C_TGCA'G
Cuts at: 830 2912 830
Size: 2082 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5560
SalI G'TCGA_C
Cuts at: 1936 2902 1936
Size: 966 4594
SbfI CC_TGCA'GG
Cuts at: 2912 2912
Size: 5560
ScaI AGT'ACT
Cuts at: 2516 3808 5520 2516
Size: 1292 1712 2556
Fragments arranged by size:
2556 1712 1292
SfcI C'TryA_G
Cuts at: 826 1045 2607 2908 4043 4724 4915 826
Size: 219 1562 301 1135 681 191 1471
Fragments arranged by size:
1562 1471 1135 681 301 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5560
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5560
SmaI CCC'GGG
Cuts at: 1872 2887 1872
Size: 1015 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3664 4565 4827 820
Size: 197 926 1721 901 262 1553
Fragments arranged by size:
1721 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3351 493
Size: 2858 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5560
SphI G_CATG'C
Cuts at: 1132 2918 1132
Size: 1786 3774
SspI AAT'ATT
Cuts at: 5 52 2771 3484 5
Size: 47 2719 713 2081
Fragments arranged by size:
2719 2081 713 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5560
StyI C'CwwG_G
Cuts at: 513 1065 2999 513
Size: 552 1934 3074
Fragments arranged by size:
3074 1934 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3587 3604 3757 3942 4387 325
Size: 1255 2007 17 153 185 445 1498
Fragments arranged by size:
2007 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2514 3806 5518
Size: 274 80 33 51 1443 537 1292 1712
Cuts at: 5518 96
Size: 138
Fragments arranged by size:
1712 1443 1292 537 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2731 2763 5520 5552 2731
Size: 32 2757 32 2739
Fragments arranged by size:
2757 2739 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5560
XbaI T'CTAG_A
Cuts at: 2896 2896
Size: 5560
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2810 2810
Size: 5560
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5560
XmaI C'CCGG_G
Cuts at: 1870 2885 1870
Size: 1015 4545
XmnI GAAnn'nnTTC
Cuts at: 3689 3689
Size: 5560
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3368 276
Size: 53 83 186 936 1834 2468
Fragments arranged by size:
2468 1834 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI