Restriction map for
pFN21AE6644
(Circular) (Six-base) MAPSORT of: pFN21AE6644.seq Check: 9343 from: 1 to: 5431
pFN21AE6644 5431 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2596 2596
Size: 5431
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3241 278
Size: 53 83 186 936 1705 2468
Fragments arranged by size:
2468 1705 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2774 1937
Size: 837 4594
Acc65I G'GTAC_C
Cuts at: 2752 2752
Size: 5431
AclI AA'CG_TT
Cuts at: 1563 2730 3558 3931 1563
Size: 1167 828 373 3063
Fragments arranged by size:
3063 1167 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2002 3494 4878 5146 1632
Size: 370 1492 1384 268 1917
Fragments arranged by size:
1917 1492 1384 370 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5234
AflIII A'CryG_T
Cuts at: 1203 2280 4330 5307 1203
Size: 1077 2050 977 1327
Fragments arranged by size:
2050 1327 1077 977
AgeI A'CCGG_T
Cuts at: 4262 4262
Size: 5431
AhdI GACnn_n'nnGTC
Cuts at: 4160 4160
Size: 5431
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5431
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5431
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2321 2353 2321
Size: 32 5399
AlwNI CAG_nnn'CTG
Cuts at: 3174 4746 3174
Size: 1572 3859
ApaI G_GGCC'C
Cuts at: 2104 2187 2665 2104
Size: 83 478 4870
Fragments arranged by size:
4870 478 83
ApaLI G'TGCA_C
Cuts at: 2639 3488 4644 2639
Size: 849 1156 3426
Fragments arranged by size:
3426 1156 849
ApoI r'AATT_y
Cuts at: 1493 2740 2966 3118 5227 1493
Size: 1247 226 152 2109 1697
Fragments arranged by size:
2109 1697 1247 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3985 160
Size: 872 2953 1606
Fragments arranged by size:
2953 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5431
AvaI C'yCGr_G
Cuts at: 1870 1943 2104 2169 2202 2261 2310 2361 2756
Size: 73 161 65 33 59 49 51 395
Cuts at: 2756 1870
Size: 4545
Fragments arranged by size:
4545 395 161 73 65 59 51 49
33
BamHI G'GATC_C
Cuts at: 2421 2761 3128 2421
Size: 340 367 4724
Fragments arranged by size:
4724 367 340
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2109 2752 4207 618
Size: 325 217 627 322 643 1455 1842
Fragments arranged by size:
1842 1455 643 627 325 322 217
BanII G_rGCy'C
Cuts at: 1391 2104 2187 2268 2665 2750 1391
Size: 713 83 81 397 85 4072
Fragments arranged by size:
4072 713 397 85 83 81
BbeI G_GCGC'C
Cuts at: 2113 2113
Size: 5431
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4460
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2252 2286 3622 3656 1903
Size: 34 50 34 231 34 1336 34 3678
Fragments arranged by size:
3678 1336 231 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3323 4533 3323
Size: 1210 4221
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5431
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4042 136
Size: 107 122 71 631 605 2370 1525
Fragments arranged by size:
2370 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5426 5426
Size: 5431
BlpI GC'TnA_GC
Cuts at: 2848 2848
Size: 5431
Bme1580I G_kGCm'C
Cuts at: 1790 2104 2187 2643 2665 3492 4648 1790
Size: 314 83 456 22 827 1156 2573
Fragments arranged by size:
2573 1156 827 456 314 83 22
BmgBI CAC'GTC
Cuts at: 1318 2320 1318
Size: 1002 4429
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2470 3162 4120
Size: 416 53 83 399 24 1053 692 958
Cuts at: 4120 442
Size: 1753
Fragments arranged by size:
1753 1053 958 692 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5431
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2014 2323 4091 1239
Size: 775 309 1768 2579
Fragments arranged by size:
2579 1768 775 309
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3556 4421 4719 3556
Size: 865 298 4268
Fragments arranged by size:
4268 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2363 4094 882
Size: 617 864 1731 2219
Fragments arranged by size:
2219 1731 864 617
BsaAI yAC'GTr
Cuts at: 493 1204 2281 2638 3222 493
Size: 711 1077 357 584 2702
Fragments arranged by size:
2702 1077 711 584 357
BsaBI GATnn'nnATC
Cuts at: 5425 5425
Size: 5431
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2054 2110 3238 3620
Size: 53 83 186 936 521 56 1128 382
Cuts at: 3620 4255 275
Size: 635 1451
Fragments arranged by size:
1451 1128 936 635 521 382 186 83
56 53
BsaWI w'CCGG_w
Cuts at: 3864 4262 4536 4683 5094 3864
Size: 398 274 147 411 4201
Fragments arranged by size:
4201 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2213 2243 2321 2351 1178
Size: 30 165 30 810 30 78 30 4258
Fragments arranged by size:
4258 810 165 78 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2015 2155 2381 2427 1186
Size: 829 140 226 46 4190
Fragments arranged by size:
4190 829 226 140 46
BseYI C'CCAG_C
Cuts at: 4634 5130 4634
Size: 496 4935
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4858
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2881 3642 4670 1540
Size: 275 186 880 761 1028 2301
Fragments arranged by size:
2301 1028 880 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2268 2379 2494 2643 3492 3577 4648 2268
Size: 111 115 149 849 85 1071 3051
Fragments arranged by size:
3051 1071 849 149 115 111 85
BsmI GAATG_Cn'
Cuts at: 2942 3035 2942
Size: 93 5338
BsmBI CGTCTCn'nnnn_
Cuts at: 2061 2061
Size: 5431
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2104 2187 2268 2379 2494 2643 2665
Size: 399 314 83 81 111 115 149 22
Cuts at: 2665 2750 3492 3577 4648 1391
Size: 85 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 314 149 115 111
85 85 83 81 22
BspEI T'CCGG_A
Cuts at: 5094 5094
Size: 5431
BspHI T'CATG_A
Cuts at: 3320 5050 3320
Size: 1730 3701
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2596 2786 844
Size: 1752 190 3489
Fragments arranged by size:
3489 1752 190
BsrBI CCG'CTC
Cuts at: 1394 1823 3318 1394
Size: 429 1495 3507
Fragments arranged by size:
3507 1495 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3926 4100 65
Size: 1183 2678 174 1396
Fragments arranged by size:
2678 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2192 4075 4262 1700
Size: 110 382 1883 187 2869
Fragments arranged by size:
2869 1883 382 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5431
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5431
BssSI C'ACGA_G
Cuts at: 3491 4503 3491
Size: 1012 4419
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5431
BstBI TT'CG_AA
Cuts at: 4235 4235
Size: 5431
BstEII G'GTnAC_C
Cuts at: 1184 2558 2630 1184
Size: 1374 72 3985
Fragments arranged by size:
3985 1374 72
BstYI r'GATC_y
Cuts at: 1972 2421 2761 3128 3513 3530 4982 5068 5080
Size: 449 340 367 385 17 1452 86 12
Cuts at: 5080 5426 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 449 385 367 346 340 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2292 2481 513
Size: 552 1227 189 3463
Fragments arranged by size:
3463 1227 552 189
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4692
Fragments arranged by size:
4692 683 56
BtsI GCAGTG_nn'
Cuts at: 2947 3741 3761 2947
Size: 794 20 4617
Fragments arranged by size:
4617 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5431
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5396
DraI TTT'AAA
Cuts at: 2735 2735
Size: 5431
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4438 809
Size: 507 3122 1802
Fragments arranged by size:
3122 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2151 2878 3767
Size: 54 1425 50 275 18 321 727 889
Cuts at: 3767 5396 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 727 321 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2878 1537
Size: 275 1066 4090
Fragments arranged by size:
4090 1066 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3361 1353
Size: 2008 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2414 2696 4018 4392 4538 387
Size: 1064 138 825 282 1322 374 146 1280
Fragments arranged by size:
1322 1280 1064 825 374 282 146 138
EcoICRI GAG'CTC
Cuts at: 2266 2266
Size: 5431
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2002 2014 2323 3494 4091 4878 5146
Size: 393 370 12 309 1171 597 787 268
Cuts at: 5146 1239
Size: 1524
Fragments arranged by size:
1524 1171 787 597 393 370 309 268
12
EcoNI CCTnn'n_nnAGG
Cuts at: 2017 2017
Size: 5431
EcoO109I rG'GnC_Cy
Cuts at: 2101 2173 2661 2662 2101
Size: 72 488 1 4870
Fragments arranged by size:
4870 488 72 1
EcoRI G'AATT_C
Cuts at: 2740 3118 2740
Size: 378 5053
FspI TGC'GCA
Cuts at: 3937 3937
Size: 5431
HaeII r_GCGC'y
Cuts at: 1678 2113 4578 1678
Size: 435 2465 2531
Fragments arranged by size:
2531 2465 435
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2406 2438 4072 4104 4146
Size: 32 135 32 834 32 1634 32 42
Cuts at: 4146 4178 1373
Size: 32 2626
Fragments arranged by size:
2626 1634 834 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2775 3021 669
Size: 988 281 837 246 3079
Fragments arranged by size:
3079 988 837 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5431
HpaI GTT'AAC
Cuts at: 3021 3021
Size: 5431
KasI G'GCGC_C
Cuts at: 2109 2109
Size: 5431
KpnI G_GTAC'C
Cuts at: 2756 2756
Size: 5431
MfeI C'AATT_G
Cuts at: 3030 3030
Size: 5431
MluI A'CGCG_T
Cuts at: 5307 5307
Size: 5431
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4545 4729 1305
Size: 3240 184 2007
Fragments arranged by size:
3240 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3609
Fragments arranged by size:
3609 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3389 3748 3907 518
Size: 648 2223 359 159 2042
Fragments arranged by size:
2223 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2092 2847 3524 4672 4917 1606
Size: 486 755 677 1148 245 2120
Fragments arranged by size:
2120 1148 755 677 486 245
NaeI GCC'GGC
Cuts at: 2194 2194
Size: 5431
NarI GG'CG_CC
Cuts at: 2110 2110
Size: 5431
NcoI C'CATG_G
Cuts at: 513 1065 2292 2481 513
Size: 552 1227 189 3463
Fragments arranged by size:
3463 1227 552 189
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5431
NgoMIV G'CCGG_C
Cuts at: 2192 2192
Size: 5431
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5431
NotI GC'GGCC_GC
Cuts at: 2878 2878
Size: 5431
NspI r_CATG'y
Cuts at: 1132 1229 2480 2789 4334 1132
Size: 97 1251 309 1545 2229
Fragments arranged by size:
2229 1545 1251 309 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5431
PciI A'CATG_T
Cuts at: 4330 4330
Size: 5431
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5431
PfoI T'CCnGG_A
Cuts at: 2580 2580
Size: 5431
PmeI GTTT'AAAC
Cuts at: 2735 2735
Size: 5431
PmlI CAC'GTG
Cuts at: 2638 2638
Size: 5431
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2321 2353 3500 3532 5013 5045 2321
Size: 32 1147 32 1481 32 2707
Fragments arranged by size:
2707 1481 1147 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2173 2173
Size: 5431
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5431
PsiI TTA'TAA
Cuts at: 3001 3001
Size: 5431
PspOMI G'GGCC_C
Cuts at: 2100 2183 2661 2100
Size: 83 478 4870
Fragments arranged by size:
4870 478 83
PspXI vC'TCGA_Gb
Cuts at: 2261 2261
Size: 5431
PstI C_TGCA'G
Cuts at: 830 2403 2433 2783 830
Size: 1573 30 350 3478
Fragments arranged by size:
3478 1573 350 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5431
SacI G_AGCT'C
Cuts at: 2268 2268
Size: 5431
SalI G'TCGA_C
Cuts at: 1936 2773 1936
Size: 837 4594
SanDI GG'GwC_CC
Cuts at: 2173 2173
Size: 5431
SbfI CC_TGCA'GG
Cuts at: 2433 2783 2433
Size: 350 5081
ScaI AGT'ACT
Cuts at: 3679 5391 3679
Size: 1712 3719
SfcI C'TryA_G
Cuts at: 826 1045 2399 2429 2779 3914 4595 4786 826
Size: 219 1354 30 350 1135 681 191 1471
Fragments arranged by size:
1471 1354 1135 681 350 219 191 30
SfoI GGC'GCC
Cuts at: 2111 2111
Size: 5431
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5431
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5431
SmaI CCC'GGG
Cuts at: 1872 2106 2171 2312 2363 2758 1872
Size: 234 65 141 51 395 4545
Fragments arranged by size:
4545 395 234 141 65 51
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2261 3535 4436 4698 820
Size: 197 926 318 1274 901 262 1553
Fragments arranged by size:
1553 1274 926 901 318 262 197
SnaBI TAC'GTA
Cuts at: 493 3222 493
Size: 2729 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5431
SphI G_CATG'C
Cuts at: 1132 2480 2789 1132
Size: 1348 309 3774
Fragments arranged by size:
3774 1348 309
SspI AAT'ATT
Cuts at: 5 52 3355 5
Size: 47 3303 2081
Fragments arranged by size:
3303 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5431
StyI C'CwwG_G
Cuts at: 513 1065 2115 2292 2481 2707 2870 513
Size: 552 1050 177 189 226 163 3074
Fragments arranged by size:
3074 1050 552 226 189 177 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2387 3458 3475 3628 3813 4258 325
Size: 1255 807 1071 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1071 807 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2283 3677 5389
Size: 274 80 33 51 1443 306 1394 1712
Cuts at: 5389 96
Size: 138
Fragments arranged by size:
1712 1443 1394 306 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2213 2245 5391 5423 2213
Size: 32 3146 32 2221
Fragments arranged by size:
3146 2221 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5431
XbaI T'CTAG_A
Cuts at: 2767 2767
Size: 5431
XhoI C'TCGA_G
Cuts at: 1943 2261 1943
Size: 318 5113
XmaI C'CCGG_G
Cuts at: 1870 2104 2169 2310 2361 2756 1870
Size: 234 65 141 51 395 4545
Fragments arranged by size:
4545 395 234 141 65 51
XmnI GAAnn'nnTTC
Cuts at: 2333 3560 2333
Size: 1227 4204
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3239 276
Size: 53 83 186 936 1705 2468
Fragments arranged by size:
2468 1705 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI SacI SalI SanDI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI NruI NsiI PacI PsrI PvuII RsrII SacII
SapI SexAI SfiI SrfI SwaI XcmI