Restriction map for
pFN21AE6642
(Circular) (Six-base) MAPSORT of: pFN21AE6642.seq Check: 2086 from: 1 to: 5551
pFN21AE6642 5551 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3361 278
Size: 53 83 186 936 1825 2468
Fragments arranged by size:
2468 1825 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2894 1937
Size: 957 4594
Acc65I G'GTAC_C
Cuts at: 2872 2872
Size: 5551
AclI AA'CG_TT
Cuts at: 1563 3678 4051 1563
Size: 2115 373 3063
Fragments arranged by size:
3063 2115 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3614 4998 5266 1632
Size: 1982 1384 268 1917
Fragments arranged by size:
1982 1917 1384 268
AfeI AGC'GCT
Cuts at: 2329 2329
Size: 5551
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5354
AflIII A'CryG_T
Cuts at: 1203 4450 5427 1203
Size: 3247 977 1327
Fragments arranged by size:
3247 1327 977
AgeI A'CCGG_T
Cuts at: 4382 4382
Size: 5551
AhdI GACnn_n'nnGTC
Cuts at: 4280 4280
Size: 5551
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5551
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5551
AlwNI CAG_nnn'CTG
Cuts at: 2205 3294 4866 2205
Size: 1089 1572 2890
Fragments arranged by size:
2890 1572 1089
ApaLI G'TGCA_C
Cuts at: 3608 4764 3608
Size: 1156 4395
ApoI r'AATT_y
Cuts at: 1493 2691 2860 3086 3238 5347 1493
Size: 1198 169 226 152 2109 1697
Fragments arranged by size:
2109 1697 1198 226 169 152
AseI AT'TA_AT
Cuts at: 160 1032 4105 160
Size: 872 3073 1606
Fragments arranged by size:
3073 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5551
AvaI C'yCGr_G
Cuts at: 1870 1943 2876 1870
Size: 73 933 4545
Fragments arranged by size:
4545 933 73
BamHI G'GATC_C
Cuts at: 2881 3248 2881
Size: 367 5184
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2081 2872 4327 618
Size: 325 217 627 294 791 1455 1842
Fragments arranged by size:
1842 1455 791 627 325 294 217
BanII G_rGCy'C
Cuts at: 1391 2870 1391
Size: 1479 4072
BbeI G_GCGC'C
Cuts at: 2085 2085
Size: 5551
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2589 928
Size: 971 690 3890
Fragments arranged by size:
3890 971 690
BbvCI CC'TCA_GC
Cuts at: 2286 2286
Size: 5551
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3742 3776 1903
Size: 34 1805 34 3678
Fragments arranged by size:
3678 1805 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3443 4653 3443
Size: 1210 4341
BclI T'GATC_A
Cuts at: 1548 2721 1548
Size: 1173 4378
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4162 136
Size: 107 122 71 631 605 2490 1525
Fragments arranged by size:
2490 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5546 5546
Size: 5551
BlpI GC'TnA_GC
Cuts at: 2968 2968
Size: 5551
Bme1580I G_kGCm'C
Cuts at: 1790 3612 4768 1790
Size: 1822 1156 2573
Fragments arranged by size:
2573 1822 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5551
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3282 4240 442
Size: 416 53 83 399 24 1865 958 1753
Fragments arranged by size:
1865 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5551
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2253 2253
Size: 5551
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2161 4211 1239
Size: 922 2050 2579
Fragments arranged by size:
2579 2050 922
Bpu10I CC'TnA_GC
Cuts at: 2286 2286
Size: 5551
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3676 4541 4839 3676
Size: 865 298 4388
Fragments arranged by size:
4388 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2059 4214 882
Size: 617 560 2155 2219
Fragments arranged by size:
2219 2155 617 560
BsaAI yAC'GTr
Cuts at: 493 1204 3342 493
Size: 711 2138 2702
Fragments arranged by size:
2702 2138 711
BsaBI GATnn'nnATC
Cuts at: 5545 5545
Size: 5551
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2082 3358 3740 4375
Size: 53 83 186 936 549 1276 382 635
Cuts at: 4375 275
Size: 1451
Fragments arranged by size:
1451 1276 936 635 549 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3984 4382 4656 4803 5214 3984
Size: 398 274 147 411 4321
Fragments arranged by size:
4321 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2159 2189 1178
Size: 30 165 30 756 30 4540
Fragments arranged by size:
4540 756 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 1186
Size: 871 4680
BseYI C'CCAG_C
Cuts at: 2024 4754 5250 2024
Size: 2730 496 2325
Fragments arranged by size:
2730 2325 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4978
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3001 3762 4790 1540
Size: 275 186 1000 761 1028 2301
Fragments arranged by size:
2301 1028 1000 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3612 3697 4768 3612
Size: 85 1071 4395
Fragments arranged by size:
4395 1071 85
BsmI GAATG_Cn'
Cuts at: 2251 3062 3155 2251
Size: 811 93 4647
Fragments arranged by size:
4647 811 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2870 3612 3697 4768 1391
Size: 399 1080 742 85 1071 2174
Fragments arranged by size:
2174 1080 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5214 5214
Size: 5551
BspHI T'CATG_A
Cuts at: 3440 5170 3440
Size: 1730 3821
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2577 2906 844
Size: 1733 329 3489
Fragments arranged by size:
3489 1733 329
BsrBI CCG'CTC
Cuts at: 1394 1823 2087 2366 3438 1394
Size: 429 264 279 1072 3507
Fragments arranged by size:
3507 1072 429 279 264
BsrDI GCAATG_nn'
Cuts at: 65 1248 4046 4220 65
Size: 1183 2798 174 1396
Fragments arranged by size:
2798 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4195 4382 1700
Size: 110 2385 187 2869
Fragments arranged by size:
2869 2385 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5551
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5551
BssSI C'ACGA_G
Cuts at: 3611 4623 3611
Size: 1012 4539
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5551
BstBI TT'CG_AA
Cuts at: 4355 4355
Size: 5551
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5551
BstXI CCAn_nnnn'nTGG
Cuts at: 2032 2257 2032
Size: 225 5326
BstYI r'GATC_y
Cuts at: 1972 2881 3248 3633 3650 5102 5188 5200 5546
Size: 909 367 385 17 1452 86 12 346
Cuts at: 5546 1972
Size: 1977
Fragments arranged by size:
1977 1452 909 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2648 2648
Size: 5551
BtgI C'CryG_G
Cuts at: 513 1065 2493 513
Size: 552 1428 3571
Fragments arranged by size:
3571 1428 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4812
Fragments arranged by size:
4812 683 56
BtsI GCAGTG_nn'
Cuts at: 2168 2305 3067 3861 3881 2168
Size: 137 762 794 20 3838
Fragments arranged by size:
3838 794 762 137 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5551
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5516
DraI TTT'AAA
Cuts at: 2855 2855
Size: 5551
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4558 809
Size: 507 3242 1802
Fragments arranged by size:
3242 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2380 2515 2998
Size: 54 1425 50 275 18 550 135 483
Cuts at: 2998 3887 5516 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 550 483 275 135 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2998 1537
Size: 275 1186 4090
Fragments arranged by size:
4090 1186 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2112 2735 2741 3481 1353
Size: 759 623 6 740 3423
Fragments arranged by size:
3423 759 740 623 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2617 4138 4512 4658 387
Size: 1064 138 1028 1521 374 146 1280
Fragments arranged by size:
1521 1280 1064 1028 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2161 3614 4211 4998 5266 1239
Size: 393 529 1453 597 787 268 1524
Fragments arranged by size:
1524 1453 787 597 529 393 268
EcoRI G'AATT_C
Cuts at: 2691 2860 3238 2691
Size: 169 378 5004
Fragments arranged by size:
5004 378 169
FspI TGC'GCA
Cuts at: 4057 4057
Size: 5551
HaeII r_GCGC'y
Cuts at: 1678 2031 2085 2331 4698 1678
Size: 353 54 246 2367 2531
Fragments arranged by size:
2531 2367 353 246 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4192 4224 4266 4298 1373
Size: 32 135 32 2620 32 42 32 2626
Fragments arranged by size:
2626 2620 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2895 3141 669
Size: 988 281 957 246 3079
Fragments arranged by size:
3079 988 957 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5551
HpaI GTT'AAC
Cuts at: 3141 3141
Size: 5551
KasI G'GCGC_C
Cuts at: 2081 2081
Size: 5551
KpnI G_GTAC'C
Cuts at: 2876 2876
Size: 5551
MfeI C'AATT_G
Cuts at: 3150 3150
Size: 5551
MluI A'CGCG_T
Cuts at: 5427 5427
Size: 5551
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4665 4849 1305
Size: 3360 184 2007
Fragments arranged by size:
3360 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2382 10
Size: 54 1425 343 550 3179
Fragments arranged by size:
3179 1425 550 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2354 2714 2729 3509 3868 4027 518
Size: 648 1188 360 15 780 359 159 2042
Fragments arranged by size:
2042 1188 780 648 360 359 159 15
MspA1I CmG'CkG
Cuts at: 1606 2783 2967 3644 4792 5037 1606
Size: 1177 184 677 1148 245 2120
Fragments arranged by size:
2120 1177 1148 677 245 184
NarI GG'CG_CC
Cuts at: 2082 2082
Size: 5551
NcoI C'CATG_G
Cuts at: 513 1065 2493 513
Size: 552 1428 3571
Fragments arranged by size:
3571 1428 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5551
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5551
NotI GC'GGCC_GC
Cuts at: 2998 2998
Size: 5551
NspI r_CATG'y
Cuts at: 1132 1229 2909 4454 1132
Size: 97 1680 1545 2229
Fragments arranged by size:
2229 1680 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2072 1348
Size: 724 4827
PciI A'CATG_T
Cuts at: 4450 4450
Size: 5551
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5551
PmeI GTTT'AAAC
Cuts at: 2855 2855
Size: 5551
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3620 3652 5133 5165 3620
Size: 32 1481 32 4006
Fragments arranged by size:
4006 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5551
PsiI TTA'TAA
Cuts at: 3121 3121
Size: 5551
PstI C_TGCA'G
Cuts at: 830 2163 2574 2903 830
Size: 1333 411 329 3478
Fragments arranged by size:
3478 1333 411 329
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5551
PvuII CAG'CTG
Cuts at: 2783 2783
Size: 5551
SalI G'TCGA_C
Cuts at: 1936 2893 1936
Size: 957 4594
SbfI CC_TGCA'GG
Cuts at: 2903 2903
Size: 5551
ScaI AGT'ACT
Cuts at: 3799 5511 3799
Size: 1712 3839
SfcI C'TryA_G
Cuts at: 826 1045 2159 2570 2899 4034 4715 4906 826
Size: 219 1114 411 329 1135 681 191 1471
Fragments arranged by size:
1471 1135 1114 681 411 329 219 191
SfoI GGC'GCC
Cuts at: 2083 2083
Size: 5551
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5551
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5551
SmaI CCC'GGG
Cuts at: 1872 2878 1872
Size: 1006 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3655 4556 4818 820
Size: 197 926 1712 901 262 1553
Fragments arranged by size:
1712 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3342 493
Size: 2849 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5551
SphI G_CATG'C
Cuts at: 1132 2909 1132
Size: 1777 3774
SspI AAT'ATT
Cuts at: 5 52 3475 5
Size: 47 3423 2081
Fragments arranged by size:
3423 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5551
StyI C'CwwG_G
Cuts at: 513 1065 2099 2493 2990 513
Size: 552 1034 394 497 3074
Fragments arranged by size:
3074 1034 552 497 394
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2469 3578 3595 3748 3933 4378 325
Size: 1255 889 1109 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1109 889 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2262 3797 5509
Size: 274 80 33 51 1443 285 1535 1712
Cuts at: 5509 96
Size: 138
Fragments arranged by size:
1712 1535 1443 285 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5511 5543 5511
Size: 32 5519
Tth111I GACn'n_nGTC
Cuts at: 1316 2501 1316
Size: 1185 4366
XbaI T'CTAG_A
Cuts at: 2887 2887
Size: 5551
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2463 2463
Size: 5551
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5551
XmaI C'CCGG_G
Cuts at: 1870 2876 1870
Size: 1006 4545
XmnI GAAnn'nnTTC
Cuts at: 3680 3680
Size: 5551
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3359 276
Size: 53 83 186 936 1825 2468
Fragments arranged by size:
2468 1825 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI