Restriction map for
pFN21AE6640
(Circular) (Six-base) MAPSORT of: pFN21AE6640.seq Check: 218 from: 1 to: 5383
pFN21AE6640 5383 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3193 278
Size: 53 83 186 936 1657 2468
Fragments arranged by size:
2468 1657 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2076 2726 1937
Size: 139 650 4594
Fragments arranged by size:
4594 650 139
Acc65I G'GTAC_C
Cuts at: 2704 2704
Size: 5383
AclI AA'CG_TT
Cuts at: 1563 3510 3883 1563
Size: 1947 373 3063
Fragments arranged by size:
3063 1947 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3446 4830 5098 1632
Size: 1814 1384 268 1917
Fragments arranged by size:
1917 1814 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5186
AflIII A'CryG_T
Cuts at: 1203 2226 2316 4282 5259 1203
Size: 1023 90 1966 977 1327
Fragments arranged by size:
1966 1327 1023 977 90
AgeI A'CCGG_T
Cuts at: 4214 4214
Size: 5383
AhdI GACnn_n'nnGTC
Cuts at: 4112 4112
Size: 5383
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5383
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5383
AlwNI CAG_nnn'CTG
Cuts at: 2101 3126 4698 2101
Size: 1025 1572 2786
Fragments arranged by size:
2786 1572 1025
ApaI G_GGCC'C
Cuts at: 2087 2252 2087
Size: 165 5218
ApaLI G'TGCA_C
Cuts at: 3440 4596 3440
Size: 1156 4227
ApoI r'AATT_y
Cuts at: 1493 2692 2918 3070 5179 1493
Size: 1199 226 152 2109 1697
Fragments arranged by size:
2109 1697 1199 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3937 160
Size: 872 2905 1606
Fragments arranged by size:
2905 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5383
AvaI C'yCGr_G
Cuts at: 1870 1943 2251 2525 2613 2708 1870
Size: 73 308 274 88 95 4545
Fragments arranged by size:
4545 308 274 95 88 73
BamHI G'GATC_C
Cuts at: 2713 3080 2713
Size: 367 5016
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2091 2363 2420 2476 2627
Size: 325 217 627 304 272 57 56 151
Cuts at: 2627 2704 4159 618
Size: 77 1455 1842
Fragments arranged by size:
1842 1455 627 325 304 272 217 151
77 57 56
BanII G_rGCy'C
Cuts at: 1391 2087 2252 2702 1391
Size: 696 165 450 4072
Fragments arranged by size:
4072 696 450 165
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2154 928
Size: 971 255 4157
Fragments arranged by size:
4157 971 255
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3574 3608 1903
Size: 34 50 34 1553 34 3678
Fragments arranged by size:
3678 1553 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3275 4485 3275
Size: 1210 4173
BclI T'GATC_A
Cuts at: 1548 2277 1548
Size: 729 4654
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2307 2395 2625
Size: 107 122 71 631 605 635 88 230
Cuts at: 2625 3994 136
Size: 1369 1525
Fragments arranged by size:
1525 1369 635 631 605 230 122 107
88 71
BglII A'GATC_T
Cuts at: 5378 5378
Size: 5383
BlpI GC'TnA_GC
Cuts at: 2800 2800
Size: 5383
Bme1580I G_kGCm'C
Cuts at: 1790 2087 2252 2423 2630 3444 4600 1790
Size: 297 165 171 207 814 1156 2573
Fragments arranged by size:
2573 1156 814 297 207 171 165
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5383
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3114 4072 442
Size: 416 53 83 399 24 1697 958 1753
Fragments arranged by size:
1753 1697 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5383
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2626 2626
Size: 5383
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2606 2693 4043 1239
Size: 1367 87 1350 2579
Fragments arranged by size:
2579 1367 1350 87
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3508 4373 4671 3508
Size: 865 298 4220
Fragments arranged by size:
4220 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4046 882
Size: 617 2547 2219
Fragments arranged by size:
2547 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2229 2317 3174 493
Size: 711 1025 88 857 2702
Fragments arranged by size:
2702 1025 857 711 88
BsaBI GATnn'nnATC
Cuts at: 5377 5377
Size: 5383
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2151 3190 3572 4207
Size: 53 83 186 936 618 1039 382 635
Cuts at: 4207 275
Size: 1451
Fragments arranged by size:
1451 1039 936 635 618 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3816 4214 4488 4635 5046 3816
Size: 398 274 147 411 4153
Fragments arranged by size:
4153 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2576 2606 1178
Size: 30 165 30 1173 30 3955
Fragments arranged by size:
3955 1173 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2045 1186
Size: 859 4524
BseYI C'CCAG_C
Cuts at: 2119 4586 5082 2119
Size: 2467 496 2420
Fragments arranged by size:
2467 2420 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4810
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2833 3594 4622 1540
Size: 275 186 832 761 1028 2301
Fragments arranged by size:
2301 1028 832 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3444 3529 4600 3444
Size: 85 1071 4227
Fragments arranged by size:
4227 1071 85
BsmI GAATG_Cn'
Cuts at: 2894 2987 2894
Size: 93 5290
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2252 2423 2630 2702 3444 3529
Size: 399 297 165 171 207 72 742 85
Cuts at: 3529 4600 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 297 207 171 165
85 72
BspEI T'CCGG_A
Cuts at: 5046 5046
Size: 5383
BspHI T'CATG_A
Cuts at: 3272 5002 3272
Size: 1730 3653
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2738 844
Size: 1894 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3270 1394
Size: 429 1447 3507
Fragments arranged by size:
3507 1447 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3878 4052 65
Size: 1183 2630 174 1396
Fragments arranged by size:
2630 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2680 4027 4214 1700
Size: 110 870 1347 187 2869
Fragments arranged by size:
2869 1347 870 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5383
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5383
BssSI C'ACGA_G
Cuts at: 3443 4455 3443
Size: 1012 4371
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5383
BstBI TT'CG_AA
Cuts at: 4187 4187
Size: 5383
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5383
BstYI r'GATC_y
Cuts at: 1972 2713 3080 3465 3482 4934 5020 5032 5378
Size: 741 367 385 17 1452 86 12 346
Cuts at: 5378 1972
Size: 1977
Fragments arranged by size:
1977 1452 741 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2215 2302 513
Size: 552 1150 87 3594
Fragments arranged by size:
3594 1150 552 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4644
Fragments arranged by size:
4644 683 56
BtsI GCAGTG_nn'
Cuts at: 2899 3693 3713 2899
Size: 794 20 4569
Fragments arranged by size:
4569 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5383
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5348
DraI TTT'AAA
Cuts at: 2687 2687
Size: 5383
DraIII CAC_nnn'GTG
Cuts at: 2202 2202
Size: 5383
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4390 809
Size: 507 3074 1802
Fragments arranged by size:
3074 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2830 3719 5348
Size: 54 1425 50 275 18 1000 889 1629
Cuts at: 5348 8
Size: 43
Fragments arranged by size:
1629 1425 1000 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2830 1537
Size: 275 1018 4090
Fragments arranged by size:
4090 1018 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2063 3313 1353
Size: 710 1250 3423
Fragments arranged by size:
3423 1250 710
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3970 4344 4490 387
Size: 1064 138 2381 374 146 1280
Fragments arranged by size:
2381 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2606 2693 3446 4043 4830 5098 1239
Size: 393 974 87 753 597 787 268 1524
Fragments arranged by size:
1524 974 787 753 597 393 268 87
EcoO109I rG'GnC_Cy
Cuts at: 2084 2098 2116 2260 2397 2617 2084
Size: 14 18 144 137 220 4850
Fragments arranged by size:
4850 220 144 137 18 14
EcoRI G'AATT_C
Cuts at: 2692 3070 2692
Size: 378 5005
FspI TGC'GCA
Cuts at: 3889 3889
Size: 5383
HaeII r_GCGC'y
Cuts at: 1678 4530 1678
Size: 2852 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2576 2608 4024 4056 4098
Size: 32 135 32 1004 32 1416 32 42
Cuts at: 4098 4130 1373
Size: 32 2626
Fragments arranged by size:
2626 1416 1004 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2727 2973 669
Size: 988 281 789 246 3079
Fragments arranged by size:
3079 988 789 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5383
HpaI GTT'AAC
Cuts at: 2973 2973
Size: 5383
KpnI G_GTAC'C
Cuts at: 2708 2708
Size: 5383
MfeI C'AATT_G
Cuts at: 2982 2982
Size: 5383
MluI A'CGCG_T
Cuts at: 5259 5259
Size: 5383
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2511 4497 4681 1305
Size: 1206 1986 184 2007
Fragments arranged by size:
2007 1986 1206 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3561
Fragments arranged by size:
3561 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2315 3341 3700 3859 518
Size: 648 1149 1026 359 159 2042
Fragments arranged by size:
2042 1149 1026 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2671 2799 3476 4624 4869 1606
Size: 1065 128 677 1148 245 2120
Fragments arranged by size:
2120 1148 1065 677 245 128
NcoI C'CATG_G
Cuts at: 513 1065 2215 2302 513
Size: 552 1150 87 3594
Fragments arranged by size:
3594 1150 552 87
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5383
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5383
NotI GC'GGCC_GC
Cuts at: 2830 2830
Size: 5383
NspI r_CATG'y
Cuts at: 1132 1229 2741 4286 1132
Size: 97 1512 1545 2229
Fragments arranged by size:
2229 1545 1512 97
PasI CC'CwG_GG
Cuts at: 1348 2087 1348
Size: 739 4644
PciI A'CATG_T
Cuts at: 4282 4282
Size: 5383
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2641 1108
Size: 1533 3850
PfoI T'CCnGG_A
Cuts at: 2017 2328 2403 2017
Size: 311 75 4997
Fragments arranged by size:
4997 311 75
PmeI GTTT'AAAC
Cuts at: 2687 2687
Size: 5383
PmlI CAC'GTG
Cuts at: 2229 2229
Size: 5383
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3452 3484 4965 4997 3452
Size: 32 1481 32 3838
Fragments arranged by size:
3838 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2116 2260 2116
Size: 144 5239
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5383
PsiI TTA'TAA
Cuts at: 2953 2953
Size: 5383
PspOMI G'GGCC_C
Cuts at: 2083 2248 2083
Size: 165 5218
PstI C_TGCA'G
Cuts at: 830 2735 830
Size: 1905 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5383
PvuII CAG'CTG
Cuts at: 2671 2671
Size: 5383
SalI G'TCGA_C
Cuts at: 1936 2725 1936
Size: 789 4594
SbfI CC_TGCA'GG
Cuts at: 2735 2735
Size: 5383
ScaI AGT'ACT
Cuts at: 3631 5343 3631
Size: 1712 3671
SexAI A'CCwGG_T
Cuts at: 2198 2198
Size: 5383
SfcI C'TryA_G
Cuts at: 826 1045 2731 3866 4547 4738 826
Size: 219 1686 1135 681 191 1471
Fragments arranged by size:
1686 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5383
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5383
SmaI CCC'GGG
Cuts at: 1872 2253 2615 2710 1872
Size: 381 362 95 4545
Fragments arranged by size:
4545 381 362 95
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3487 4388 4650 820
Size: 197 926 1544 901 262 1553
Fragments arranged by size:
1553 1544 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3174 493
Size: 2681 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5383
SphI G_CATG'C
Cuts at: 1132 2741 1132
Size: 1609 3774
SrfI GCCC'GGGC
Cuts at: 2253 2253
Size: 5383
SspI AAT'ATT
Cuts at: 5 52 3307 5
Size: 47 3255 2081
Fragments arranged by size:
3255 2081 47
StuI AGG'CCT
Cuts at: 1517 2574 1517
Size: 1057 4326
StyI C'CwwG_G
Cuts at: 513 1065 2215 2302 2822 513
Size: 552 1150 87 520 3074
Fragments arranged by size:
3074 1150 552 520 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2079 3410 3427 3580 3765 4210 325
Size: 1255 499 1331 17 153 185 445 1498
Fragments arranged by size:
1498 1331 1255 499 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3629 5341 96
Size: 274 80 33 51 1443 1652 1712 138
Fragments arranged by size:
1712 1652 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5343 5375 5343
Size: 32 5351
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5383
XbaI T'CTAG_A
Cuts at: 2719 2719
Size: 5383
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2401 2401
Size: 5383
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5383
XmaI C'CCGG_G
Cuts at: 1870 2251 2613 2708 1870
Size: 381 362 95 4545
Fragments arranged by size:
4545 381 362 95
XmnI GAAnn'nnTTC
Cuts at: 3512 3512
Size: 5383
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3191 276
Size: 53 83 186 936 1657 2468
Fragments arranged by size:
2468 1657 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI
SacII SanDI SapI SfiI SfoI SwaI