Restriction map for
pFN21AE6637
(Circular) (Six-base) MAPSORT of: pFN21AE6637.seq Check: 4927 from: 1 to: 5350
pFN21AE6637 5350 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3160 278
Size: 53 83 186 936 1624 2468
Fragments arranged by size:
2468 1624 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2693 1937
Size: 756 4594
Acc65I G'GTAC_C
Cuts at: 2671 2671
Size: 5350
AclI AA'CG_TT
Cuts at: 1563 3477 3850 1563
Size: 1914 373 3063
Fragments arranged by size:
3063 1914 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2222 3413 4797 5065 1632
Size: 590 1191 1384 268 1917
Fragments arranged by size:
1917 1384 1191 590 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5153
AflIII A'CryG_T
Cuts at: 1203 4249 5226 1203
Size: 3046 977 1327
Fragments arranged by size:
3046 1327 977
AgeI A'CCGG_T
Cuts at: 4181 4181
Size: 5350
AhdI GACnn_n'nnGTC
Cuts at: 4079 4079
Size: 5350
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5350
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5350
AlwNI CAG_nnn'CTG
Cuts at: 2032 2157 2247 3093 4665 2032
Size: 125 90 846 1572 2717
Fragments arranged by size:
2717 1572 846 125 90
ApaLI G'TGCA_C
Cuts at: 3407 4563 3407
Size: 1156 4194
ApoI r'AATT_y
Cuts at: 1493 2659 2885 3037 5146 1493
Size: 1166 226 152 2109 1697
Fragments arranged by size:
2109 1697 1166 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3904 160
Size: 872 2872 1606
Fragments arranged by size:
2872 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5350
AvaI C'yCGr_G
Cuts at: 1870 1943 2675 1870
Size: 73 732 4545
Fragments arranged by size:
4545 732 73
BamHI G'GATC_C
Cuts at: 2680 3047 2680
Size: 367 4983
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2216 2671 4126 618
Size: 325 217 627 429 455 1455 1842
Fragments arranged by size:
1842 1455 627 455 429 325 217
BanII G_rGCy'C
Cuts at: 1391 2603 2669 1391
Size: 1212 66 4072
Fragments arranged by size:
4072 1212 66
BbeI G_GCGC'C
Cuts at: 2220 2220
Size: 5350
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4379
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3541 3575 1903
Size: 34 1604 34 3678
Fragments arranged by size:
3678 1604 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3242 4452 3242
Size: 1210 4140
BclI T'GATC_A
Cuts at: 1548 2113 1548
Size: 565 4785
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3961 136
Size: 107 122 71 631 605 2289 1525
Fragments arranged by size:
2289 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2424 5345 2424
Size: 2921 2429
BlpI GC'TnA_GC
Cuts at: 2019 2767 2019
Size: 748 4602
Bme1580I G_kGCm'C
Cuts at: 1790 3411 4567 1790
Size: 1621 1156 2573
Fragments arranged by size:
2573 1621 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5350
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3081 4039 442
Size: 416 53 83 399 24 1664 958 1753
Fragments arranged by size:
1753 1664 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5350
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2617 2617
Size: 5350
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2288 2320 4010 1239
Size: 1049 32 1690 2579
Fragments arranged by size:
2579 1690 1049 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3475 4340 4638 3475
Size: 865 298 4187
Fragments arranged by size:
4187 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2003 4013 882
Size: 617 504 2010 2219
Fragments arranged by size:
2219 2010 617 504
BsaAI yAC'GTr
Cuts at: 493 1204 3141 493
Size: 711 1937 2702
Fragments arranged by size:
2702 1937 711
BsaBI GATnn'nnATC
Cuts at: 2105 2384 5344 2105
Size: 279 2960 2111
Fragments arranged by size:
2960 2111 279
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2217 2413 3157 3539
Size: 53 83 186 936 684 196 744 382
Cuts at: 3539 4174 275
Size: 635 1451
Fragments arranged by size:
1451 936 744 684 635 382 196 186
83 53
BsaWI w'CCGG_w
Cuts at: 2193 3783 4181 4455 4602 5013 2193
Size: 1590 398 274 147 411 2530
Fragments arranged by size:
2530 1590 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2293 2323 2426 2456 1178
Size: 30 165 30 890 30 103 30 4072
Fragments arranged by size:
4072 890 165 103 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2289 2586 1186
Size: 1103 297 3950
Fragments arranged by size:
3950 1103 297
BseYI C'CCAG_C
Cuts at: 2289 4553 5049 2289
Size: 2264 496 2590
Fragments arranged by size:
2590 2264 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4777
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2800 3561 4589 1540
Size: 275 186 799 761 1028 2301
Fragments arranged by size:
2301 1028 799 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3411 3496 4567 3411
Size: 85 1071 4194
Fragments arranged by size:
4194 1071 85
BsmI GAATG_Cn'
Cuts at: 2861 2954 2861
Size: 93 5257
BsmBI CGTCTCn'nnnn_
Cuts at: 2336 2336
Size: 5350
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2603 2669 3411 3496 4567 1391
Size: 399 813 66 742 85 1071 2174
Fragments arranged by size:
2174 1071 813 742 399 85 66
BspEI T'CCGG_A
Cuts at: 5013 5013
Size: 5350
BspHI T'CATG_A
Cuts at: 3239 4969 3239
Size: 1730 3620
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2705 844
Size: 1861 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3237 1394
Size: 429 1414 3507
Fragments arranged by size:
3507 1414 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3845 4019 65
Size: 1183 2597 174 1396
Fragments arranged by size:
2597 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3994 4181 1700
Size: 110 2184 187 2869
Fragments arranged by size:
2869 2184 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5350
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5350
BssSI C'ACGA_G
Cuts at: 3410 4422 3410
Size: 1012 4338
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5350
BstBI TT'CG_AA
Cuts at: 4154 4154
Size: 5350
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5350
BstXI CCAn_nnnn'nTGG
Cuts at: 2056 2525 2056
Size: 469 4881
BstYI r'GATC_y
Cuts at: 1972 2424 2622 2680 3047 3432 3449 4901 4987
Size: 452 198 58 367 385 17 1452 86
Cuts at: 4987 4999 5345 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 452 385 367 346 198 86
58 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2093 2454 513
Size: 552 939 89 361 3409
Fragments arranged by size:
3409 939 552 361 89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4611
Fragments arranged by size:
4611 683 56
BtsI GCAGTG_nn'
Cuts at: 2866 3660 3680 2866
Size: 794 20 4536
Fragments arranged by size:
4536 794 20
ClaI AT'CG_AT
Cuts at: 1554 2630 1554
Size: 1076 4274
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5315
DraI TTT'AAA
Cuts at: 2654 2654
Size: 5350
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4357 809
Size: 507 3041 1802
Fragments arranged by size:
3041 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2797 3686 5315
Size: 54 1425 50 275 18 967 889 1629
Cuts at: 5315 8
Size: 43
Fragments arranged by size:
1629 1425 967 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2797 1537
Size: 275 985 4090
Fragments arranged by size:
4090 985 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3280 1353
Size: 1927 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2333 3937 4311 4457 387
Size: 1064 138 744 1604 374 146 1280
Fragments arranged by size:
1604 1280 1064 744 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2222 2288 2320 3413 4010 4797 5065
Size: 393 590 66 32 1093 597 787 268
Cuts at: 5065 1239
Size: 1524
Fragments arranged by size:
1524 1093 787 597 590 393 268 66
32
EcoO109I rG'GnC_Cy
Cuts at: 2082 2502 2082
Size: 420 4930
EcoRI G'AATT_C
Cuts at: 2659 3037 2659
Size: 378 4972
EcoRV GAT'ATC
Cuts at: 2531 2531
Size: 5350
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2419 2419
Size: 5350
FspI TGC'GCA
Cuts at: 3856 3856
Size: 5350
HaeII r_GCGC'y
Cuts at: 1678 2220 4497 1678
Size: 542 2277 2531
Fragments arranged by size:
2531 2277 542
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2426 2458 3991 4023 4065
Size: 32 135 32 854 32 1533 32 42
Cuts at: 4065 4097 1373
Size: 32 2626
Fragments arranged by size:
2626 1533 854 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2694 2940 669
Size: 988 281 756 246 3079
Fragments arranged by size:
3079 988 756 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5350
HpaI GTT'AAC
Cuts at: 2940 2940
Size: 5350
KasI G'GCGC_C
Cuts at: 2216 2216
Size: 5350
KpnI G_GTAC'C
Cuts at: 2675 2675
Size: 5350
MfeI C'AATT_G
Cuts at: 2949 2949
Size: 5350
MluI A'CGCG_T
Cuts at: 5226 5226
Size: 5350
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2069 2668 4464 4648 1305
Size: 764 599 1796 184 2007
Fragments arranged by size:
2007 1796 764 599 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3528
Fragments arranged by size:
3528 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2054 3308 3667 3826 518
Size: 648 888 1254 359 159 2042
Fragments arranged by size:
2042 1254 888 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2766 3443 4591 4836 1606
Size: 1160 677 1148 245 2120
Fragments arranged by size:
2120 1160 1148 677 245
NarI GG'CG_CC
Cuts at: 2217 2217
Size: 5350
NcoI C'CATG_G
Cuts at: 513 1065 2004 2093 2454 513
Size: 552 939 89 361 3409
Fragments arranged by size:
3409 939 552 361 89
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5350
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5350
NotI GC'GGCC_GC
Cuts at: 2797 2797
Size: 5350
NspI r_CATG'y
Cuts at: 1132 1229 2708 4253 1132
Size: 97 1479 1545 2229
Fragments arranged by size:
2229 1545 1479 97
PasI CC'CwG_GG
Cuts at: 1348 2086 1348
Size: 738 4612
PciI A'CATG_T
Cuts at: 4249 4249
Size: 5350
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2460 1108
Size: 1352 3998
PfoI T'CCnGG_A
Cuts at: 2265 2524 2265
Size: 259 5091
PmeI GTTT'AAAC
Cuts at: 2654 2654
Size: 5350
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3419 3451 4932 4964 3419
Size: 32 1481 32 3805
Fragments arranged by size:
3805 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2082 2082
Size: 5350
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5350
PsiI TTA'TAA
Cuts at: 2920 2920
Size: 5350
PstI C_TGCA'G
Cuts at: 830 2702 830
Size: 1872 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5350
SalI G'TCGA_C
Cuts at: 1936 2692 1936
Size: 756 4594
SanDI GG'GwC_CC
Cuts at: 2082 2082
Size: 5350
SbfI CC_TGCA'GG
Cuts at: 2702 2702
Size: 5350
ScaI AGT'ACT
Cuts at: 3598 5310 3598
Size: 1712 3638
SfcI C'TryA_G
Cuts at: 826 1045 2698 3833 4514 4705 826
Size: 219 1653 1135 681 191 1471
Fragments arranged by size:
1653 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2218 2218
Size: 5350
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5350
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5350
SmaI CCC'GGG
Cuts at: 1872 2677 1872
Size: 805 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3454 4355 4617 820
Size: 197 926 1511 901 262 1553
Fragments arranged by size:
1553 1511 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3141 493
Size: 2648 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5350
SphI G_CATG'C
Cuts at: 1132 2708 1132
Size: 1576 3774
SspI AAT'ATT
Cuts at: 5 52 3274 5
Size: 47 3222 2081
Fragments arranged by size:
3222 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5350
StyI C'CwwG_G
Cuts at: 513 1065 2004 2093 2454 2789 513
Size: 552 939 89 361 335 3074
Fragments arranged by size:
3074 939 552 361 335 89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3377 3394 3547 3732 4177 325
Size: 1255 1797 17 153 185 445 1498
Fragments arranged by size:
1797 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3596 5308 96
Size: 274 80 33 51 1443 1619 1712 138
Fragments arranged by size:
1712 1619 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5310 5342 5310
Size: 32 5318
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5350
XbaI T'CTAG_A
Cuts at: 2686 2686
Size: 5350
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5350
XmaI C'CCGG_G
Cuts at: 1870 2675 1870
Size: 805 4545
XmnI GAAnn'nnTTC
Cuts at: 2040 3479 2040
Size: 1439 3911
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3158 276
Size: 53 83 186 936 1624 2468
Fragments arranged by size:
2468 1624 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI
SexAI SfiI SrfI SwaI XcmI