Restriction map for
pFN21AE6635
(Circular) (Six-base) MAPSORT of: pFN21AE6635.seq Check: 8896 from: 1 to: 5446
pFN21AE6635 5446 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3256 278
Size: 53 83 186 936 1720 2468
Fragments arranged by size:
2468 1720 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2789 1937
Size: 852 4594
Acc65I G'GTAC_C
Cuts at: 2398 2767 2398
Size: 369 5077
AclI AA'CG_TT
Cuts at: 1563 3573 3946 1563
Size: 2010 373 3063
Fragments arranged by size:
3063 2010 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2316 3509 4893 5161 1632
Size: 684 1193 1384 268 1917
Fragments arranged by size:
1917 1384 1193 684 268
AflII C'TTAA_G
Cuts at: 820 1017 2135 820
Size: 197 1118 4131
Fragments arranged by size:
4131 1118 197
AflIII A'CryG_T
Cuts at: 1203 4345 5322 1203
Size: 3142 977 1327
Fragments arranged by size:
3142 1327 977
AgeI A'CCGG_T
Cuts at: 4277 4277
Size: 5446
AhdI GACnn_n'nnGTC
Cuts at: 4175 4175
Size: 5446
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5446
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5446
AlwNI CAG_nnn'CTG
Cuts at: 3189 4761 3189
Size: 1572 3874
ApaLI G'TGCA_C
Cuts at: 3503 4659 3503
Size: 1156 4290
ApoI r'AATT_y
Cuts at: 1493 2117 2167 2231 2353 2755 2981 3133 5242
Size: 624 50 64 122 402 226 152 2109
Cuts at: 5242 1493
Size: 1697
Fragments arranged by size:
2109 1697 624 402 226 152 122 64
50
AseI AT'TA_AT
Cuts at: 160 1032 4000 160
Size: 872 2968 1606
Fragments arranged by size:
2968 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5446
AvaI C'yCGr_G
Cuts at: 1870 1943 2031 2771 1870
Size: 73 88 740 4545
Fragments arranged by size:
4545 740 88 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2209 2242 2390 2423 2209
Size: 33 148 33 5232
Fragments arranged by size:
5232 148 33 33
BamHI G'GATC_C
Cuts at: 2650 2776 3143 2650
Size: 126 367 4953
Fragments arranged by size:
4953 367 126
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2056 2398 2767 4222 618
Size: 325 217 627 269 342 369 1455 1842
Fragments arranged by size:
1842 1455 627 369 342 325 269 217
BanII G_rGCy'C
Cuts at: 1391 2765 1391
Size: 1374 4072
BbeI G_GCGC'C
Cuts at: 2060 2060
Size: 5446
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4475
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3637 3671 1903
Size: 34 50 34 1616 34 3678
Fragments arranged by size:
3678 1616 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2210 3338 4548 2210
Size: 1128 1210 3108
Fragments arranged by size:
3108 1210 1128
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5446
BfrBI ATG'CAT
Cuts at: 2495 2495
Size: 5446
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4057 136
Size: 107 122 71 631 605 2385 1525
Fragments arranged by size:
2385 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5441 5441
Size: 5446
BlpI GC'TnA_GC
Cuts at: 2863 2863
Size: 5446
Bme1580I G_kGCm'C
Cuts at: 1790 3507 4663 1790
Size: 1717 1156 2573
Fragments arranged by size:
2573 1717 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5446
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3177 4135 442
Size: 416 53 83 399 24 1760 958 1753
Fragments arranged by size:
1760 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5446
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2242 4106 1239
Size: 1003 1864 2579
Fragments arranged by size:
2579 1864 1003
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3571 4436 4734 3571
Size: 865 298 4283
Fragments arranged by size:
4283 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4109 882
Size: 617 2610 2219
Fragments arranged by size:
2610 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3237 493
Size: 711 2033 2702
Fragments arranged by size:
2702 2033 711
BsaBI GATnn'nnATC
Cuts at: 5440 5440
Size: 5446
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2057 3253 3635 4270
Size: 53 83 186 936 524 1196 382 635
Cuts at: 4270 275
Size: 1451
Fragments arranged by size:
1451 1196 936 635 524 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3879 4277 4551 4698 5109 3879
Size: 398 274 147 411 4216
Fragments arranged by size:
4216 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5221
Fragments arranged by size:
5221 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5446
BseYI C'CCAG_C
Cuts at: 2627 4649 5145 2627
Size: 2022 496 2928
Fragments arranged by size:
2928 2022 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2631 2643 1163
Size: 573 895 12 3966
Fragments arranged by size:
3966 895 573 12
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2896 3657 4685 1540
Size: 275 186 895 761 1028 2301
Fragments arranged by size:
2301 1028 895 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2072 2575 3507 3592 4663 2072
Size: 503 932 85 1071 2855
Fragments arranged by size:
2855 1071 932 503 85
BsmI GAATG_Cn'
Cuts at: 2957 3050 2957
Size: 93 5353
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2575 2765 3507 3592 4663 1391
Size: 399 282 503 190 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 503 399 282 190 85
BspEI T'CCGG_A
Cuts at: 5109 5109
Size: 5446
BspHI T'CATG_A
Cuts at: 3335 5065 3335
Size: 1730 3716
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2801 844
Size: 1957 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3333 1394
Size: 429 1510 3507
Fragments arranged by size:
3507 1510 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3941 4115 65
Size: 1183 2693 174 1396
Fragments arranged by size:
2693 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4090 4277 1700
Size: 110 2280 187 2869
Fragments arranged by size:
2869 2280 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5446
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5446
BssSI C'ACGA_G
Cuts at: 3506 4518 3506
Size: 1012 4434
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2513 2710 794
Size: 1719 197 3530
Fragments arranged by size:
3530 1719 197
BstBI TT'CG_AA
Cuts at: 4250 4250
Size: 5446
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5446
BstXI CCAn_nnnn'nTGG
Cuts at: 2484 2662 2484
Size: 178 5268
BstYI r'GATC_y
Cuts at: 1972 2650 2776 3143 3528 3545 4997 5083 5095
Size: 678 126 367 385 17 1452 86 12
Cuts at: 5095 5441 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 678 385 367 346 126 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2402 513
Size: 552 1337 3557
Fragments arranged by size:
3557 1337 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4707
Fragments arranged by size:
4707 683 56
BtsI GCAGTG_nn'
Cuts at: 2962 3756 3776 2962
Size: 794 20 4632
Fragments arranged by size:
4632 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5446
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5411
DraI TTT'AAA
Cuts at: 2500 2750 2500
Size: 250 5196
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4453 809
Size: 507 3137 1802
Fragments arranged by size:
3137 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2313 2448 2893
Size: 54 1425 50 275 18 483 135 445
Cuts at: 2893 3782 5411 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 483 445 275 135 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2893 1537
Size: 275 1081 4090
Fragments arranged by size:
4090 1081 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3376 1353
Size: 2023 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4033 4407 4553 387
Size: 1064 138 2444 374 146 1280
Fragments arranged by size:
2444 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2242 2316 3509 4106 4893 5161 1239
Size: 393 610 74 1193 597 787 268 1524
Fragments arranged by size:
1524 1193 787 610 597 393 268 74
EcoO109I rG'GnC_Cy
Cuts at: 2087 2087
Size: 5446
EcoRI G'AATT_C
Cuts at: 2755 3133 2755
Size: 378 5068
FspI TGC'GCA
Cuts at: 3952 3952
Size: 5446
HaeII r_GCGC'y
Cuts at: 1678 2025 2039 2060 4593 1678
Size: 347 14 21 2533 2531
Fragments arranged by size:
2533 2531 347 21 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2377 2409 4087 4119 4161
Size: 32 135 32 805 32 1678 32 42
Cuts at: 4161 4193 1373
Size: 32 2626
Fragments arranged by size:
2626 1678 805 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2686 2790 3036 669
Size: 988 281 748 104 246 3079
Fragments arranged by size:
3079 988 748 281 246 104
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5446
HpaI GTT'AAC
Cuts at: 3036 3036
Size: 5446
KasI G'GCGC_C
Cuts at: 2056 2056
Size: 5446
KpnI G_GTAC'C
Cuts at: 2402 2771 2402
Size: 369 5077
MfeI C'AATT_G
Cuts at: 3045 3045
Size: 5446
MluI A'CGCG_T
Cuts at: 5322 5322
Size: 5446
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2194 2527 4560 4744 1305
Size: 889 333 2033 184 2007
Fragments arranged by size:
2033 2007 889 333 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2315 2450 10
Size: 54 1425 343 483 135 3006
Fragments arranged by size:
3006 1425 483 343 135 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2517 3404 3763 3922 518
Size: 648 1351 887 359 159 2042
Fragments arranged by size:
2042 1351 887 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2627 2669 2862 3539 4687 4932 1606
Size: 1021 42 193 677 1148 245 2120
Fragments arranged by size:
2120 1148 1021 677 245 193 42
NarI GG'CG_CC
Cuts at: 2057 2057
Size: 5446
NcoI C'CATG_G
Cuts at: 513 1065 2402 513
Size: 552 1337 3557
Fragments arranged by size:
3557 1337 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5446
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5446
NotI GC'GGCC_GC
Cuts at: 2893 2893
Size: 5446
NsiI A_TGCA'T
Cuts at: 2497 2497
Size: 5446
NspI r_CATG'y
Cuts at: 1132 1229 2804 4349 1132
Size: 97 1575 1545 2229
Fragments arranged by size:
2229 1575 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5446
PciI A'CATG_T
Cuts at: 4345 4345
Size: 5446
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5446
PfoI T'CCnGG_A
Cuts at: 2160 2160
Size: 5446
PmeI GTTT'AAAC
Cuts at: 2750 2750
Size: 5446
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2328 2360 2574 2606 3515 3547 5028 5060 2328
Size: 32 214 32 909 32 1481 32 2714
Fragments arranged by size:
2714 1481 909 214 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2087 2087
Size: 5446
PshAI GACnn'nnGTC
Cuts at: 1613 2638 1613
Size: 1025 4421
PsiI TTA'TAA
Cuts at: 3016 3016
Size: 5446
PstI C_TGCA'G
Cuts at: 830 2798 830
Size: 1968 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5446
PvuII CAG'CTG
Cuts at: 2627 2669 2627
Size: 42 5404
SalI G'TCGA_C
Cuts at: 1936 2788 1936
Size: 852 4594
SanDI GG'GwC_CC
Cuts at: 2087 2087
Size: 5446
SbfI CC_TGCA'GG
Cuts at: 2798 2798
Size: 5446
ScaI AGT'ACT
Cuts at: 3694 5406 3694
Size: 1712 3734
SexAI A'CCwGG_T
Cuts at: 2467 2467
Size: 5446
SfcI C'TryA_G
Cuts at: 826 1045 2794 3929 4610 4801 826
Size: 219 1749 1135 681 191 1471
Fragments arranged by size:
1749 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2058 2058
Size: 5446
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5446
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5446
SmaI CCC'GGG
Cuts at: 1872 2773 1872
Size: 901 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2135 3550 4451 4713 820
Size: 197 926 192 1415 901 262 1553
Fragments arranged by size:
1553 1415 926 901 262 197 192
SnaBI TAC'GTA
Cuts at: 493 3237 493
Size: 2744 2702
SpeI A'CTAG_T
Cuts at: 152 2542 152
Size: 2390 3056
SphI G_CATG'C
Cuts at: 1132 2804 1132
Size: 1672 3774
SspI AAT'ATT
Cuts at: 5 52 2109 3370 5
Size: 47 2057 1261 2081
Fragments arranged by size:
2081 2057 1261 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5446
StyI C'CwwG_G
Cuts at: 513 1065 2310 2402 2885 513
Size: 552 1245 92 483 3074
Fragments arranged by size:
3074 1245 552 483 92
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3473 3490 3643 3828 4273 325
Size: 1255 1893 17 153 185 445 1498
Fragments arranged by size:
1893 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3692 5404 96
Size: 274 80 33 51 1443 1715 1712 138
Fragments arranged by size:
1715 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2593 2625 5406 5438 2593
Size: 32 2781 32 2601
Fragments arranged by size:
2781 2601 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5446
XbaI T'CTAG_A
Cuts at: 2782 2782
Size: 5446
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5446
XmaI C'CCGG_G
Cuts at: 1870 2771 1870
Size: 901 4545
XmnI GAAnn'nnTTC
Cuts at: 2156 3575 2156
Size: 1419 4027
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3254 276
Size: 53 83 186 936 1720 2468
Fragments arranged by size:
2468 1720 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI
SfiI SrfI SwaI XcmI