Restriction map for
pFN21AE6633
(Circular) (Six-base) MAPSORT of: pFN21AE6633.seq Check: 7796 from: 1 to: 5653
pFN21AE6633 5653 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3463 278
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2996 1937
Size: 1059 4594
Acc65I G'GTAC_C
Cuts at: 2974 2974
Size: 5653
AclI AA'CG_TT
Cuts at: 1563 3780 4153 1563
Size: 2217 373 3063
Fragments arranged by size:
3063 2217 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2246 2833 3716 5100 5368 1632
Size: 614 587 883 1384 268 1917
Fragments arranged by size:
1917 1384 883 614 587 268
AfeI AGC'GCT
Cuts at: 2582 2582
Size: 5653
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5456
AflIII A'CryG_T
Cuts at: 1203 4552 5529 1203
Size: 3349 977 1327
Fragments arranged by size:
3349 1327 977
AgeI A'CCGG_T
Cuts at: 4484 4484
Size: 5653
AhdI GACnn_n'nnGTC
Cuts at: 4382 4382
Size: 5653
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5653
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5653
AlwNI CAG_nnn'CTG
Cuts at: 2281 2856 3396 4968 2281
Size: 575 540 1572 2966
Fragments arranged by size:
2966 1572 575 540
ApaI G_GGCC'C
Cuts at: 2887 2887
Size: 5653
ApaLI G'TGCA_C
Cuts at: 2687 3710 4866 2687
Size: 1023 1156 3474
Fragments arranged by size:
3474 1156 1023
ApoI r'AATT_y
Cuts at: 1493 2962 3188 3340 5449 1493
Size: 1469 226 152 2109 1697
Fragments arranged by size:
2109 1697 1469 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4207 160
Size: 872 3175 1606
Fragments arranged by size:
3175 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
AvaI C'yCGr_G
Cuts at: 1870 1943 2196 2978 1870
Size: 73 253 782 4545
Fragments arranged by size:
4545 782 253 73
BamHI G'GATC_C
Cuts at: 2983 3350 2983
Size: 367 5286
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2141 2663 2974 4429 618
Size: 325 217 627 354 522 311 1455 1842
Fragments arranged by size:
1842 1455 627 522 354 325 311 217
BanII G_rGCy'C
Cuts at: 1391 2181 2197 2832 2887 2972 1391
Size: 790 16 635 55 85 4072
Fragments arranged by size:
4072 790 635 85 55 16
BbeI G_GCGC'C
Cuts at: 2145 2145
Size: 5653
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4682
BbvCI CC'TCA_GC
Cuts at: 2331 2817 2331
Size: 486 5167
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3844 3878 1903
Size: 34 50 34 1823 34 3678
Fragments arranged by size:
3678 1823 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2254 3545 4755 2254
Size: 1291 1210 3152
Fragments arranged by size:
3152 1291 1210
BclI T'GATC_A
Cuts at: 1548 2133 2370 1548
Size: 585 237 4831
Fragments arranged by size:
4831 585 237
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4264 136
Size: 107 122 71 631 605 2592 1525
Fragments arranged by size:
2592 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5648 5648
Size: 5653
BlpI GC'TnA_GC
Cuts at: 2904 3070 2904
Size: 166 5487
Bme1580I G_kGCm'C
Cuts at: 1790 2691 2887 3714 4870 1790
Size: 901 196 827 1156 2573
Fragments arranged by size:
2573 1156 901 827 196
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5653
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3384 4342 442
Size: 416 53 83 399 24 1967 958 1753
Fragments arranged by size:
1967 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2187 1056
Size: 1131 4522
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2501 2552 2846 4313 1239
Size: 1262 51 294 1467 2579
Fragments arranged by size:
2579 1467 1262 294 51
Bpu10I CC'TnA_GC
Cuts at: 2331 2817 2331
Size: 486 5167
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3778 4643 4941 3778
Size: 865 298 4490
Fragments arranged by size:
4490 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4316 882
Size: 617 2817 2219
Fragments arranged by size:
2817 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3444 493
Size: 711 2240 2702
Fragments arranged by size:
2702 2240 711
BsaBI GATnn'nnATC
Cuts at: 5647 5647
Size: 5653
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2142 2697 3460 3842
Size: 53 83 186 936 609 555 763 382
Cuts at: 3842 4477 275
Size: 635 1451
Fragments arranged by size:
1451 936 763 635 609 555 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2943 4086 4484 4758 4905 5316 2943
Size: 1143 398 274 147 411 3280
Fragments arranged by size:
3280 1143 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2184 2214 1178
Size: 30 165 30 781 30 4617
Fragments arranged by size:
4617 781 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2205 2453 2716 1186
Size: 1019 248 263 4123
Fragments arranged by size:
4123 1019 263 248
BseYI C'CCAG_C
Cuts at: 4856 5352 4856
Size: 496 5157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2129 2490 2786 1163
Size: 573 393 361 296 4030
Fragments arranged by size:
4030 573 393 361 296
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3103 3864 4892 1540
Size: 275 186 1102 761 1028 2301
Fragments arranged by size:
2301 1102 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2181 2691 3714 3799 4870 2181
Size: 510 1023 85 1071 2964
Fragments arranged by size:
2964 1071 1023 510 85
BsmI GAATG_Cn'
Cuts at: 3164 3257 3164
Size: 93 5560
BsmBI CGTCTCn'nnnn_
Cuts at: 2159 2159
Size: 5653
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2181 2197 2691 2832 2887 2972 3714
Size: 399 391 16 494 141 55 85 742
Cuts at: 3714 3799 4870 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 494 399 391 141 85
85 55 16
BspEI T'CCGG_A
Cuts at: 5316 5316
Size: 5653
BspHI T'CATG_A
Cuts at: 3542 5272 3542
Size: 1730 3923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2481 3008 844
Size: 1637 527 3489
Fragments arranged by size:
3489 1637 527
BsrBI CCG'CTC
Cuts at: 1394 1823 2706 3540 1394
Size: 429 883 834 3507
Fragments arranged by size:
3507 883 834 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4148 4322 65
Size: 1183 2900 174 1396
Fragments arranged by size:
2900 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4297 4484 1700
Size: 110 2487 187 2869
Fragments arranged by size:
2869 2487 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5653
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5653
BssSI C'ACGA_G
Cuts at: 2637 3713 4725 2637
Size: 1076 1012 3565
Fragments arranged by size:
3565 1076 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5653
BstBI TT'CG_AA
Cuts at: 4457 4457
Size: 5653
BstEII G'GTnAC_C
Cuts at: 1184 2011 2671 1184
Size: 827 660 4166
Fragments arranged by size:
4166 827 660
BstYI r'GATC_y
Cuts at: 1972 2983 3350 3735 3752 5204 5290 5302 5648
Size: 1011 367 385 17 1452 86 12 346
Cuts at: 5648 1972
Size: 1977
Fragments arranged by size:
1977 1452 1011 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5101
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4914
Fragments arranged by size:
4914 683 56
BtsI GCAGTG_nn'
Cuts at: 2691 2803 3169 3963 3983 2691
Size: 112 366 794 20 4361
Fragments arranged by size:
4361 794 366 112 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5653
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5618
DraI TTT'AAA
Cuts at: 2957 2957
Size: 5653
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2872 4660 809
Size: 507 1556 1788 1802
Fragments arranged by size:
1802 1788 1556 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2169 2619 2646
Size: 54 1425 50 275 18 339 450 27
Cuts at: 2646 3100 3989 5618 8
Size: 454 889 1629 43
Fragments arranged by size:
1629 1425 889 454 450 339 275 54
50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 3100 1537
Size: 275 1288 4090
Fragments arranged by size:
4090 1288 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2759 3583 1353
Size: 1406 824 3423
Fragments arranged by size:
3423 1406 824
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4240 4614 4760 387
Size: 1064 138 2651 374 146 1280
Fragments arranged by size:
2651 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2179 2179
Size: 5653
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2246 2501 2552 2833 2846 3716 4313
Size: 393 614 255 51 281 13 870 597
Cuts at: 4313 5100 5368 1239
Size: 787 268 1524
Fragments arranged by size:
1524 870 787 614 597 393 281 268
255 51 13
EcoNI CCTnn'n_nnAGG
Cuts at: 2816 2816
Size: 5653
EcoO109I rG'GnC_Cy
Cuts at: 2821 2865 2883 2821
Size: 44 18 5591
Fragments arranged by size:
5591 44 18
EcoRI G'AATT_C
Cuts at: 2962 3340 2962
Size: 378 5275
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2572 2572
Size: 5653
FspI TGC'GCA
Cuts at: 4159 4159
Size: 5653
HaeII r_GCGC'y
Cuts at: 1678 2145 2584 2911 4800 1678
Size: 467 439 327 1889 2531
Fragments arranged by size:
2531 1889 467 439 327
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2184 2216 2297 2329 4294
Size: 32 135 32 612 32 81 32 1965
Cuts at: 4294 4326 4368 4400 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1965 612 135 81 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2443 2997 3243 669
Size: 988 281 505 554 246 3079
Fragments arranged by size:
3079 988 554 505 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5653
HpaI GTT'AAC
Cuts at: 3243 3243
Size: 5653
KasI G'GCGC_C
Cuts at: 2141 2141
Size: 5653
KpnI G_GTAC'C
Cuts at: 2978 2978
Size: 5653
MfeI C'AATT_G
Cuts at: 3252 3252
Size: 5653
MluI A'CGCG_T
Cuts at: 5529 5529
Size: 5653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2328 4767 4951 1305
Size: 1023 2439 184 2007
Fragments arranged by size:
2439 2007 1023 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2621 2648 10
Size: 54 1425 343 789 27 3015
Fragments arranged by size:
3015 1425 789 343 54 27
MslI CAynn'nnrTG
Cuts at: 518 1166 3611 3970 4129 518
Size: 648 2445 359 159 2042
Fragments arranged by size:
2445 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2414 2903 2925 3069 3746 4894 5139 1606
Size: 808 489 22 144 677 1148 245 2120
Fragments arranged by size:
2120 1148 808 677 489 245 144 22
NarI GG'CG_CC
Cuts at: 2142 2142
Size: 5653
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5101
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5653
NheI G'CTAG_C
Cuts at: 1052 2183 1052
Size: 1131 4522
NotI GC'GGCC_GC
Cuts at: 3100 3100
Size: 5653
NspI r_CATG'y
Cuts at: 1132 1229 3011 4556 1132
Size: 97 1782 1545 2229
Fragments arranged by size:
2229 1782 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5653
PciI A'CATG_T
Cuts at: 4552 4552
Size: 5653
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2281 1108
Size: 1173 4480
PfoI T'CCnGG_A
Cuts at: 2478 2478
Size: 5653
PmeI GTTT'AAAC
Cuts at: 2957 2957
Size: 5653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3722 3754 5235 5267 3722
Size: 32 1481 32 4108
Fragments arranged by size:
4108 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2865 2865
Size: 5653
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5653
PsiI TTA'TAA
Cuts at: 3223 3223
Size: 5653
PspOMI G'GGCC_C
Cuts at: 2883 2883
Size: 5653
PstI C_TGCA'G
Cuts at: 830 2216 3005 830
Size: 1386 789 3478
Fragments arranged by size:
3478 1386 789
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5653
PvuII CAG'CTG
Cuts at: 2414 2903 2925 2414
Size: 489 22 5142
Fragments arranged by size:
5142 489 22
SacI G_AGCT'C
Cuts at: 2181 2181
Size: 5653
SalI G'TCGA_C
Cuts at: 1936 2995 1936
Size: 1059 4594
SapI GCTCTTCn'nnn_
Cuts at: 2759 2759
Size: 5653
SbfI CC_TGCA'GG
Cuts at: 3005 3005
Size: 5653
ScaI AGT'ACT
Cuts at: 2846 3901 5613 2846
Size: 1055 1712 2886
Fragments arranged by size:
2886 1712 1055
SfcI C'TryA_G
Cuts at: 826 1045 2212 2584 3001 4136 4817 5008 826
Size: 219 1167 372 417 1135 681 191 1471
Fragments arranged by size:
1471 1167 1135 681 417 372 219 191
SfoI GGC'GCC
Cuts at: 2143 2143
Size: 5653
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5653
SmaI CCC'GGG
Cuts at: 1872 2980 1872
Size: 1108 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3757 4658 4920 820
Size: 197 926 1814 901 262 1553
Fragments arranged by size:
1814 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3444 493
Size: 2951 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5653
SphI G_CATG'C
Cuts at: 1132 3011 1132
Size: 1879 3774
SspI AAT'ATT
Cuts at: 5 52 3577 5
Size: 47 3525 2081
Fragments arranged by size:
3525 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5653
StyI C'CwwG_G
Cuts at: 513 1065 2016 2659 2879 3092 513
Size: 552 951 643 220 213 3074
Fragments arranged by size:
3074 951 643 552 220 213
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2706 2860 3680 3697 3850 4035 4480
Size: 1255 1126 154 820 17 153 185 445
Cuts at: 4480 325
Size: 1498
Fragments arranged by size:
1498 1255 1126 820 445 185 154 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2221 2844 3899
Size: 274 80 33 51 1443 244 623 1055
Cuts at: 3899 5611 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1055 623 274 244 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5613 5645 5613
Size: 32 5621
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5653
XbaI T'CTAG_A
Cuts at: 2989 2989
Size: 5653
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5653
XmaI C'CCGG_G
Cuts at: 1870 2978 1870
Size: 1108 4545
XmnI GAAnn'nnTTC
Cuts at: 3782 3782
Size: 5653
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3461 276
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SrfI SwaI XcmI