Restriction map for
pFN21AE6630
(Circular) (Six-base) MAPSORT of: pFN21AE6630.seq Check: 1856 from: 1 to: 5476
pFN21AE6630 5476 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3286 278
Size: 53 83 186 936 1750 2468
Fragments arranged by size:
2468 1750 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2819 1937
Size: 882 4594
Acc65I G'GTAC_C
Cuts at: 2797 2797
Size: 5476
AclI AA'CG_TT
Cuts at: 1563 2775 3603 3976 1563
Size: 1212 828 373 3063
Fragments arranged by size:
3063 1212 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3539 4923 5191 1632
Size: 1907 1384 268 1917
Fragments arranged by size:
1917 1907 1384 268
AfeI AGC'GCT
Cuts at: 2705 2705
Size: 5476
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5279
AflIII A'CryG_T
Cuts at: 1203 4375 5352 1203
Size: 3172 977 1327
Fragments arranged by size:
3172 1327 977
AgeI A'CCGG_T
Cuts at: 4307 4307
Size: 5476
AhdI GACnn_n'nnGTC
Cuts at: 4205 4205
Size: 5476
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5476
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5476
AlwNI CAG_nnn'CTG
Cuts at: 2527 3219 4791 2527
Size: 692 1572 3212
Fragments arranged by size:
3212 1572 692
ApaLI G'TGCA_C
Cuts at: 2096 3533 4689 2096
Size: 1437 1156 2883
Fragments arranged by size:
2883 1437 1156
ApoI r'AATT_y
Cuts at: 1493 2225 2785 3011 3163 5272 1493
Size: 732 560 226 152 2109 1697
Fragments arranged by size:
2109 1697 732 560 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4030 160
Size: 872 2998 1606
Fragments arranged by size:
2998 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5476
AvaI C'yCGr_G
Cuts at: 1870 1943 2801 1870
Size: 73 858 4545
Fragments arranged by size:
4545 858 73
BamHI G'GATC_C
Cuts at: 2806 3173 2806
Size: 367 5109
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2563 2797 4252 618
Size: 325 217 627 776 234 1455 1842
Fragments arranged by size:
1842 1455 776 627 325 234 217
BanII G_rGCy'C
Cuts at: 1391 2795 1391
Size: 1404 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4505
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3667 3701 1903
Size: 34 1730 34 3678
Fragments arranged by size:
3678 1730 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3368 4578 3368
Size: 1210 4266
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5476
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4087 136
Size: 107 122 71 631 605 337 2078 1525
Fragments arranged by size:
2078 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5471 5471
Size: 5476
BlpI GC'TnA_GC
Cuts at: 2893 2893
Size: 5476
Bme1580I G_kGCm'C
Cuts at: 1790 2100 3537 4693 1790
Size: 310 1437 1156 2573
Fragments arranged by size:
2573 1437 1156 310
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5476
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3207 4165 442
Size: 416 53 83 399 24 1790 958 1753
Fragments arranged by size:
1790 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5476
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2187 2187
Size: 5476
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2157 2504 2711 4136 1239
Size: 918 347 207 1425 2579
Fragments arranged by size:
2579 1425 918 347 207
Bpu10I CC'TnA_GC
Cuts at: 2027 2027
Size: 5476
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2279 2495 3601 4466 4764 2279
Size: 216 1106 865 298 2991
Fragments arranged by size:
2991 1106 865 298 216
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2015 4139 882
Size: 617 516 2124 2219
Fragments arranged by size:
2219 2124 617 516
BsaAI yAC'GTr
Cuts at: 493 1204 2743 3267 493
Size: 711 1539 524 2702
Fragments arranged by size:
2702 1539 711 524
BsaBI GATnn'nnATC
Cuts at: 2319 5470 2319
Size: 3151 2325
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3283 3665 4300 275
Size: 53 83 186 936 1750 382 635 1451
Fragments arranged by size:
1750 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2624 3909 4307 4581 4728 5139 2624
Size: 1285 398 274 147 411 2961
Fragments arranged by size:
2961 1285 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2073 2103 1178
Size: 30 165 30 670 30 4551
Fragments arranged by size:
4551 670 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2727 1186
Size: 1541 3935
BseYI C'CCAG_C
Cuts at: 4679 5175 4679
Size: 496 4980
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4903
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2926 3687 4715 1540
Size: 275 186 925 761 1028 2301
Fragments arranged by size:
2301 1028 925 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2100 3537 3622 4693 2100
Size: 1437 85 1071 2883
Fragments arranged by size:
2883 1437 1071 85
BsmI GAATG_Cn'
Cuts at: 2987 3080 2987
Size: 93 5383
BsmBI CGTCTCn'nnnn_
Cuts at: 2622 2622
Size: 5476
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2795 3537 3622 4693 1391
Size: 399 310 695 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 695 399 310 85
BspEI T'CCGG_A
Cuts at: 2624 5139 2624
Size: 2515 2961
BspHI T'CATG_A
Cuts at: 3365 5095 3365
Size: 1730 3746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2831 844
Size: 1987 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2089 3363 1394
Size: 429 266 1274 3507
Fragments arranged by size:
3507 1274 429 266
BsrDI GCAATG_nn'
Cuts at: 65 1248 3971 4145 65
Size: 1183 2723 174 1396
Fragments arranged by size:
2723 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4120 4307 1700
Size: 110 2310 187 2869
Fragments arranged by size:
2869 2310 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5476
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5476
BssSI C'ACGA_G
Cuts at: 3536 4548 3536
Size: 1012 4464
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5476
BstBI TT'CG_AA
Cuts at: 2221 2270 4280 2221
Size: 49 2010 3417
Fragments arranged by size:
3417 2010 49
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5476
BstXI CCAn_nnnn'nTGG
Cuts at: 2074 2074
Size: 5476
BstYI r'GATC_y
Cuts at: 1972 2427 2806 3173 3558 3575 5027 5113 5125
Size: 455 379 367 385 17 1452 86 12
Cuts at: 5125 5471 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 455 385 379 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3985
Fragments arranged by size:
3985 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4737
Fragments arranged by size:
4737 683 56
BtsI GCAGTG_nn'
Cuts at: 2992 3786 3806 2992
Size: 794 20 4662
Fragments arranged by size:
4662 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5476
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5441
DraI TTT'AAA
Cuts at: 2191 2780 2191
Size: 589 4887
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4483 809
Size: 507 3167 1802
Fragments arranged by size:
3167 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2923 3812 5441
Size: 54 1425 50 275 18 1093 889 1629
Cuts at: 5441 8
Size: 43
Fragments arranged by size:
1629 1425 1093 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2923 1537
Size: 275 1111 4090
Fragments arranged by size:
4090 1111 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2054 2648 3406 1353
Size: 701 594 758 3423
Fragments arranged by size:
3423 758 701 594
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4063 4437 4583 387
Size: 1064 138 2474 374 146 1280
Fragments arranged by size:
2474 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2157 2504 2711 3539 4136 4923 5191
Size: 393 525 347 207 828 597 787 268
Cuts at: 5191 1239
Size: 1524
Fragments arranged by size:
1524 828 787 597 525 393 347 268
207
EcoO109I rG'GnC_Cy
Cuts at: 2168 2515 2168
Size: 347 5129
EcoRI G'AATT_C
Cuts at: 2785 3163 2785
Size: 378 5098
FspI TGC'GCA
Cuts at: 3982 3982
Size: 5476
HaeII r_GCGC'y
Cuts at: 1678 2707 4623 1678
Size: 1029 1916 2531
Fragments arranged by size:
2531 1916 1029
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2481 2513 4117 4149 4191
Size: 32 135 32 909 32 1604 32 42
Cuts at: 4191 4223 1373
Size: 32 2626
Fragments arranged by size:
2626 1604 909 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2820 3066 669
Size: 988 281 882 246 3079
Fragments arranged by size:
3079 988 882 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5476
HpaI GTT'AAC
Cuts at: 3066 3066
Size: 5476
KpnI G_GTAC'C
Cuts at: 2801 2801
Size: 5476
MfeI C'AATT_G
Cuts at: 2250 3075 2250
Size: 825 4651
MluI A'CGCG_T
Cuts at: 5352 5352
Size: 5476
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4590 4774 1305
Size: 3285 184 2007
Fragments arranged by size:
3285 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3654
Fragments arranged by size:
3654 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3434 3793 3952 518
Size: 648 2268 359 159 2042
Fragments arranged by size:
2268 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2177 2540 2892 3569 4717 4962 1606
Size: 571 363 352 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 571 363 352 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3985
Fragments arranged by size:
3985 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5476
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5476
NotI GC'GGCC_GC
Cuts at: 2923 2923
Size: 5476
NspI r_CATG'y
Cuts at: 1132 1229 2407 2834 4379 1132
Size: 97 1178 427 1545 2229
Fragments arranged by size:
2229 1545 1178 427 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5476
PciI A'CATG_T
Cuts at: 4375 4375
Size: 5476
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2527 1108
Size: 1419 4057
PfoI T'CCnGG_A
Cuts at: 2679 2679
Size: 5476
PmeI GTTT'AAAC
Cuts at: 2780 2780
Size: 5476
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3545 3577 5058 5090 3545
Size: 32 1481 32 3931
Fragments arranged by size:
3931 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2515 2515
Size: 5476
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5476
PsiI TTA'TAA
Cuts at: 3046 3046
Size: 5476
PstI C_TGCA'G
Cuts at: 830 2182 2828 830
Size: 1352 646 3478
Fragments arranged by size:
3478 1352 646
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5476
PvuII CAG'CTG
Cuts at: 2177 2540 2177
Size: 363 5113
SalI G'TCGA_C
Cuts at: 1936 2818 1936
Size: 882 4594
SbfI CC_TGCA'GG
Cuts at: 2828 2828
Size: 5476
ScaI AGT'ACT
Cuts at: 3724 5436 3724
Size: 1712 3764
SfcI C'TryA_G
Cuts at: 826 1045 2178 2641 2824 3959 4640 4831 826
Size: 219 1133 463 183 1135 681 191 1471
Fragments arranged by size:
1471 1135 1133 681 463 219 191 183
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5476
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5476
SmaI CCC'GGG
Cuts at: 1872 2803 1872
Size: 931 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2258 2510 3580 4481 4743 820
Size: 197 926 315 252 1070 901 262 1553
Fragments arranged by size:
1553 1070 926 901 315 262 252 197
SnaBI TAC'GTA
Cuts at: 493 3267 493
Size: 2774 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5476
SphI G_CATG'C
Cuts at: 1132 2834 1132
Size: 1702 3774
SspI AAT'ATT
Cuts at: 5 52 3400 5
Size: 47 3348 2081
Fragments arranged by size:
3348 2081 47
StuI AGG'CCT
Cuts at: 1517 2558 2696 1517
Size: 1041 138 4297
Fragments arranged by size:
4297 1041 138
StyI C'CwwG_G
Cuts at: 513 1065 2004 2559 2915 513
Size: 552 939 555 356 3074
Fragments arranged by size:
3074 939 555 552 356
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3503 3520 3673 3858 4303 325
Size: 1255 1923 17 153 185 445 1498
Fragments arranged by size:
1923 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3722 5434 96
Size: 274 80 33 51 1443 1745 1712 138
Fragments arranged by size:
1745 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5436 5468 5436
Size: 32 5444
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5476
XbaI T'CTAG_A
Cuts at: 2812 2812
Size: 5476
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5476
XmaI C'CCGG_G
Cuts at: 1870 2801 1870
Size: 931 4545
XmnI GAAnn'nnTTC
Cuts at: 3605 3605
Size: 5476
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3284 276
Size: 53 83 186 936 1750 2468
Fragments arranged by size:
2468 1750 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI