Restriction map for
pFN21AE6629
(Circular) (Six-base) MAPSORT of: pFN21AE6629.seq Check: 7431 from: 1 to: 5575
pFN21AE6629 5575 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3385 278
Size: 53 83 186 936 1849 2468
Fragments arranged by size:
2468 1849 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2918 1937
Size: 981 4594
Acc65I G'GTAC_C
Cuts at: 2896 2896
Size: 5575
AclI AA'CG_TT
Cuts at: 1563 3702 4075 1563
Size: 2139 373 3063
Fragments arranged by size:
3063 2139 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2303 3638 5022 5290 1632
Size: 671 1335 1384 268 1917
Fragments arranged by size:
1917 1384 1335 671 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5378
AflIII A'CryG_T
Cuts at: 1203 2530 2770 4474 5451 1203
Size: 1327 240 1704 977 1327
Fragments arranged by size:
1704 1327 1327 977 240
AgeI A'CCGG_T
Cuts at: 4406 4406
Size: 5575
AhdI GACnn_n'nnGTC
Cuts at: 4304 4304
Size: 5575
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5575
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5575
AlwNI CAG_nnn'CTG
Cuts at: 3318 4890 3318
Size: 1572 4003
ApaLI G'TGCA_C
Cuts at: 3632 4788 3632
Size: 1156 4419
ApoI r'AATT_y
Cuts at: 1493 2750 2884 3110 3262 5371 1493
Size: 1257 134 226 152 2109 1697
Fragments arranged by size:
2109 1697 1257 226 152 134
AseI AT'TA_AT
Cuts at: 160 1032 4129 160
Size: 872 3097 1606
Fragments arranged by size:
3097 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5575
AvaI C'yCGr_G
Cuts at: 1870 1943 2045 2900 1870
Size: 73 102 855 4545
Fragments arranged by size:
4545 855 102 73
BamHI G'GATC_C
Cuts at: 2905 3272 2905
Size: 367 5208
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2896 4351 618
Size: 325 217 627 1109 1455 1842
Fragments arranged by size:
1842 1455 1109 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2424 2894 1391
Size: 1033 470 4072
Fragments arranged by size:
4072 1033 470
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4604
BbvCI CC'TCA_GC
Cuts at: 2762 2762
Size: 5575
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3766 3800 1903
Size: 34 1829 34 3678
Fragments arranged by size:
3678 1829 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3467 4677 3467
Size: 1210 4365
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5575
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2323 4186 136
Size: 107 122 71 631 605 651 1863 1525
Fragments arranged by size:
1863 1525 651 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5570 5570
Size: 5575
BlpI GC'TnA_GC
Cuts at: 2992 2992
Size: 5575
Bme1580I G_kGCm'C
Cuts at: 1790 3636 4792 1790
Size: 1846 1156 2573
Fragments arranged by size:
2573 1846 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5575
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2368 2659 3306
Size: 416 53 83 399 24 951 291 647
Cuts at: 3306 4264 442
Size: 958 1753
Fragments arranged by size:
1753 958 951 647 416 399 291 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5575
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4235 1239
Size: 2996 2579
Bpu10I CC'TnA_GC
Cuts at: 2015 2762 2015
Size: 747 4828
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2155 2304 3700 4565 4863 2155
Size: 149 1396 865 298 2867
Fragments arranged by size:
2867 1396 865 298 149
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4238 882
Size: 617 2739 2219
Fragments arranged by size:
2739 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3366 493
Size: 711 2162 2702
Fragments arranged by size:
2702 2162 711
BsaBI GATnn'nnATC
Cuts at: 5569 5569
Size: 5575
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3382 3764 4399 275
Size: 53 83 186 936 1849 382 635 1451
Fragments arranged by size:
1849 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4008 4406 4680 4827 5238 4008
Size: 398 274 147 411 4345
Fragments arranged by size:
4345 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2426 2456 1178
Size: 30 165 30 1023 30 4297
Fragments arranged by size:
4297 1023 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 2090 1186
Size: 892 12 4671
Fragments arranged by size:
4671 892 12
BseYI C'CCAG_C
Cuts at: 2733 4778 5274 2733
Size: 2045 496 3034
Fragments arranged by size:
3034 2045 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5002
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3025 3786 4814 1540
Size: 275 186 1024 761 1028 2301
Fragments arranged by size:
2301 1028 1024 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2391 2424 3636 3721 4792 2391
Size: 33 1212 85 1071 3174
Fragments arranged by size:
3174 1212 1071 85 33
BsmI GAATG_Cn'
Cuts at: 3086 3179 3086
Size: 93 5482
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2391 2424 2894 3636 3721 4792 1391
Size: 399 601 33 470 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 601 470 399 85 33
BspEI T'CCGG_A
Cuts at: 5238 5238
Size: 5575
BspHI T'CATG_A
Cuts at: 3464 5194 3464
Size: 1730 3845
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2930 844
Size: 2086 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3462 1394
Size: 429 1639 3507
Fragments arranged by size:
3507 1639 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2312 2499 4070 4244 65
Size: 1183 1064 187 1571 174 1396
Fragments arranged by size:
1571 1396 1183 1064 187 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4219 4406 1700
Size: 110 2409 187 2869
Fragments arranged by size:
2869 2409 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5575
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5575
BssSI C'ACGA_G
Cuts at: 3635 4647 3635
Size: 1012 4563
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5575
BstBI TT'CG_AA
Cuts at: 2755 4379 2755
Size: 1624 3951
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5575
BstYI r'GATC_y
Cuts at: 1972 2905 3272 3657 3674 5126 5212 5224 5570
Size: 933 367 385 17 1452 86 12 346
Cuts at: 5570 1972
Size: 1977
Fragments arranged by size:
1977 1452 933 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2415 513
Size: 552 939 411 3673
Fragments arranged by size:
3673 939 552 411
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4836
Fragments arranged by size:
4836 683 56
BtsI GCAGTG_nn'
Cuts at: 2692 3091 3885 3905 2692
Size: 399 794 20 4362
Fragments arranged by size:
4362 794 399 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5575
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2208 2243 2434 2469 529
Size: 35 1644 35 191 35 3635
Fragments arranged by size:
3635 1644 191 35 35 35
DraI TTT'AAA
Cuts at: 2879 2879
Size: 5575
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2246 4582 809
Size: 507 930 2336 1802
Fragments arranged by size:
2336 1802 930 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3022 3911 5540
Size: 54 1425 50 275 18 1192 889 1629
Cuts at: 5540 8
Size: 43
Fragments arranged by size:
1629 1425 1192 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3022 1537
Size: 275 1210 4090
Fragments arranged by size:
4090 1210 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2049 2067 2359 3505 1353
Size: 696 18 292 1146 3423
Fragments arranged by size:
3423 1146 696 292 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2198 4162 4536 4682 387
Size: 1064 138 435 174 1964 374 146 1280
Fragments arranged by size:
1964 1280 1064 435 374 174 146 138
EcoICRI GAG'CTC
Cuts at: 2422 2422
Size: 5575
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2303 3638 4235 5022 5290 1239
Size: 393 671 1335 597 787 268 1524
Fragments arranged by size:
1524 1335 787 671 597 393 268
EcoRI G'AATT_C
Cuts at: 2884 3262 2884
Size: 378 5197
FspI TGC'GCA
Cuts at: 4081 4081
Size: 5575
HaeII r_GCGC'y
Cuts at: 1678 4722 1678
Size: 3044 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4216 4248 4290 4322 1373
Size: 32 135 32 2644 32 42 32 2626
Fragments arranged by size:
2644 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2919 3165 669
Size: 988 281 981 246 3079
Fragments arranged by size:
3079 988 981 281 246
HindIII A'AGCT_T
Cuts at: 748 2406 748
Size: 1658 3917
HpaI GTT'AAC
Cuts at: 3165 3165
Size: 5575
KpnI G_GTAC'C
Cuts at: 2900 2900
Size: 5575
MfeI C'AATT_G
Cuts at: 3174 3174
Size: 5575
MluI A'CGCG_T
Cuts at: 5451 5451
Size: 5575
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2181 4689 4873 1305
Size: 876 2508 184 2007
Fragments arranged by size:
2508 2007 876 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3753
Fragments arranged by size:
3753 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3533 3892 4051 518
Size: 648 2367 359 159 2042
Fragments arranged by size:
2367 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2991 3668 4816 5061 1606
Size: 1385 677 1148 245 2120
Fragments arranged by size:
2120 1385 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2415 513
Size: 552 939 411 3673
Fragments arranged by size:
3673 939 552 411
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5575
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5575
NotI GC'GGCC_GC
Cuts at: 3022 3022
Size: 5575
NspI r_CATG'y
Cuts at: 1132 1229 2534 2774 2933 4478 1132
Size: 97 1305 240 159 1545 2229
Fragments arranged by size:
2229 1545 1305 240 159 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5575
PciI A'CATG_T
Cuts at: 2530 2770 4474 2530
Size: 240 1704 3631
Fragments arranged by size:
3631 1704 240
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5575
PmeI GTTT'AAAC
Cuts at: 2879 2879
Size: 5575
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3644 3676 5157 5189 3644
Size: 32 1481 32 4030
Fragments arranged by size:
4030 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5575
PsiI TTA'TAA
Cuts at: 3145 3145
Size: 5575
PstI C_TGCA'G
Cuts at: 830 2927 830
Size: 2097 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5575
SacI G_AGCT'C
Cuts at: 2424 2424
Size: 5575
SalI G'TCGA_C
Cuts at: 1936 2917 1936
Size: 981 4594
SbfI CC_TGCA'GG
Cuts at: 2927 2927
Size: 5575
ScaI AGT'ACT
Cuts at: 3823 5535 3823
Size: 1712 3863
SfcI C'TryA_G
Cuts at: 826 1045 2738 2923 4058 4739 4930 826
Size: 219 1693 185 1135 681 191 1471
Fragments arranged by size:
1693 1471 1135 681 219 191 185
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5575
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5575
SmaI CCC'GGG
Cuts at: 1872 2902 1872
Size: 1030 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2170 2319 3679 4580 4842 820
Size: 197 926 227 149 1360 901 262 1553
Fragments arranged by size:
1553 1360 926 901 262 227 197 149
SnaBI TAC'GTA
Cuts at: 493 3366 493
Size: 2873 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5575
SphI G_CATG'C
Cuts at: 1132 2933 1132
Size: 1801 3774
SspI AAT'ATT
Cuts at: 5 52 3499 5
Size: 47 3447 2081
Fragments arranged by size:
3447 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5575
StyI C'CwwG_G
Cuts at: 513 1065 2004 2415 3014 513
Size: 552 939 411 599 3074
Fragments arranged by size:
3074 939 599 552 411
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2641 3602 3619 3772 3957 4402 325
Size: 1255 1061 961 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1061 961 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3821 5533 96
Size: 274 80 33 51 1443 1844 1712 138
Fragments arranged by size:
1844 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2426 2458 5535 5567 2426
Size: 32 3077 32 2434
Fragments arranged by size:
3077 2434 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2852 1316
Size: 1536 4039
XbaI T'CTAG_A
Cuts at: 2911 2911
Size: 5575
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5575
XmaI C'CCGG_G
Cuts at: 1870 2900 1870
Size: 1030 4545
XmnI GAAnn'nnTTC
Cuts at: 2751 3704 2751
Size: 953 4622
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3383 276
Size: 53 83 186 936 1849 2468
Fragments arranged by size:
2468 1849 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI