Restriction map for
pFN21AE6626
(Circular) (Six-base) MAPSORT of: pFN21AE6626.seq Check: 4552 from: 1 to: 5647
pFN21AE6626 5647 bp
With 182 enzymes: SgfI *
June 20, 2019 11:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3457 278
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2990 1937
Size: 1053 4594
Acc65I G'GTAC_C
Cuts at: 2968 2968
Size: 5647
AclI AA'CG_TT
Cuts at: 1563 3774 4147 1563
Size: 2211 373 3063
Fragments arranged by size:
3063 2211 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2600 3710 5094 5362 1632
Size: 968 1110 1384 268 1917
Fragments arranged by size:
1917 1384 1110 968 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5450
AflIII A'CryG_T
Cuts at: 1203 4546 5523 1203
Size: 3343 977 1327
Fragments arranged by size:
3343 1327 977
AgeI A'CCGG_T
Cuts at: 4478 4478
Size: 5647
AhdI GACnn_n'nnGTC
Cuts at: 4376 4376
Size: 5647
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5647
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5647
AlwNI CAG_nnn'CTG
Cuts at: 2758 3390 4962 2758
Size: 632 1572 3443
Fragments arranged by size:
3443 1572 632
ApaI G_GGCC'C
Cuts at: 2160 2160
Size: 5647
ApaLI G'TGCA_C
Cuts at: 3704 4860 3704
Size: 1156 4491
ApoI r'AATT_y
Cuts at: 1493 2956 3182 3334 5443 1493
Size: 1463 226 152 2109 1697
Fragments arranged by size:
2109 1697 1463 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4201 160
Size: 872 3169 1606
Fragments arranged by size:
3169 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
AvaI C'yCGr_G
Cuts at: 1870 1943 2447 2972 1870
Size: 73 504 525 4545
Fragments arranged by size:
4545 525 504 73
BamHI G'GATC_C
Cuts at: 2977 3344 2977
Size: 367 5280
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2011 2199 2968 4423 618
Size: 325 217 627 224 188 769 1455 1842
Fragments arranged by size:
1842 1455 769 627 325 224 217 188
BanII G_rGCy'C
Cuts at: 1391 2160 2245 2966 1391
Size: 769 85 721 4072
Fragments arranged by size:
4072 769 721 85
BbeI G_GCGC'C
Cuts at: 2015 2203 2015
Size: 188 5459
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2706 928
Size: 971 807 3869
Fragments arranged by size:
3869 971 807
BbvCI CC'TCA_GC
Cuts at: 2766 2766
Size: 5647
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3838 3872 1903
Size: 34 1901 34 3678
Fragments arranged by size:
3678 1901 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3539 4749 3539
Size: 1210 4437
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5647
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2840 4258
Size: 107 122 71 631 605 337 831 1418
Cuts at: 4258 136
Size: 1525
Fragments arranged by size:
1525 1418 831 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 5642 5642
Size: 5647
BlpI GC'TnA_GC
Cuts at: 3064 3064
Size: 5647
Bme1580I G_kGCm'C
Cuts at: 1790 2160 3708 4864 1790
Size: 370 1548 1156 2573
Fragments arranged by size:
2573 1548 1156 370
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5647
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3378 4336 442
Size: 416 53 83 399 24 1961 958 1753
Fragments arranged by size:
1961 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5647
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2183 2205 2399 2519 4307 1239
Size: 944 22 194 120 1788 2579
Fragments arranged by size:
2579 1788 944 194 120 22
Bpu10I CC'TnA_GC
Cuts at: 2766 2766
Size: 5647
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3772 4637 4935 3772
Size: 865 298 4484
Fragments arranged by size:
4484 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2612 4310 882
Size: 617 1113 1698 2219
Fragments arranged by size:
2219 1698 1113 617
BsaAI yAC'GTr
Cuts at: 493 1204 3438 493
Size: 711 2234 2702
Fragments arranged by size:
2702 2234 711
BsaBI GATnn'nnATC
Cuts at: 5641 5641
Size: 5647
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 2200 3454 3836
Size: 53 83 186 936 479 188 1254 382
Cuts at: 3836 4471 275
Size: 635 1451
Fragments arranged by size:
1451 1254 936 635 479 382 188 186
83 53
BsaWI w'CCGG_w
Cuts at: 4080 4478 4752 4899 5310 4080
Size: 398 274 147 411 4417
Fragments arranged by size:
4417 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5422
Fragments arranged by size:
5422 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 2186 2294 2321 2396 2432 2447 2495
Size: 949 51 108 27 75 36 15 48
Cuts at: 2495 2498 2516 2519 2531 2534 2537 2555 2579
Size: 3 18 3 12 3 3 18 24
Cuts at: 2579 2615 1186
Size: 36 4218
Fragments arranged by size:
4218 949 108 75 51 48 36 36
27 24 18 18 15 12 3 3
3 3
BseYI C'CCAG_C
Cuts at: 2148 2232 2244 2403 2454 2550 2941 4850 5346
Size: 84 12 159 51 96 391 1909 496
Cuts at: 5346 2148
Size: 2449
Fragments arranged by size:
2449 1909 496 391 159 96 84 51
12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2096 1163
Size: 573 360 4714
Fragments arranged by size:
4714 573 360
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2797 3097 3858 4886 1540
Size: 275 186 796 300 761 1028 2301
Fragments arranged by size:
2301 1028 796 761 300 275 186
BsiHKAI G_wGCw'C
Cuts at: 2694 3708 3793 4864 2694
Size: 1014 85 1071 3477
Fragments arranged by size:
3477 1071 1014 85
BsmI GAATG_Cn'
Cuts at: 3158 3251 3158
Size: 93 5554
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2160 2245 2694 2966 3708 3793 4864
Size: 399 370 85 449 272 742 85 1071
Cuts at: 4864 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 449 399 370 272 85
85
BspEI T'CCGG_A
Cuts at: 5310 5310
Size: 5647
BspHI T'CATG_A
Cuts at: 3536 5266 3536
Size: 1730 3917
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2766 3002 844
Size: 1922 236 3489
Fragments arranged by size:
3489 1922 236
BsrBI CCG'CTC
Cuts at: 1394 1823 2437 2488 3534 1394
Size: 429 614 51 1046 3507
Fragments arranged by size:
3507 1046 614 429 51
BsrDI GCAATG_nn'
Cuts at: 65 1248 4142 4316 65
Size: 1183 2894 174 1396
Fragments arranged by size:
2894 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2096 4291 4478 1700
Size: 110 286 2195 187 2869
Fragments arranged by size:
2869 2195 286 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5647
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5647
BssSI C'ACGA_G
Cuts at: 3707 4719 3707
Size: 1012 4635
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5647
BstBI TT'CG_AA
Cuts at: 4451 4451
Size: 5647
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5647
BstYI r'GATC_y
Cuts at: 1972 2977 3344 3729 3746 5198 5284 5296 5642
Size: 1005 367 385 17 1452 86 12 346
Cuts at: 5642 1972
Size: 1977
Fragments arranged by size:
1977 1452 1005 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2169 2169
Size: 5647
BtgI C'CryG_G
Cuts at: 513 1065 2004 2838 513
Size: 552 939 834 3322
Fragments arranged by size:
3322 939 834 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2860 487
Size: 683 56 1634 3274
Fragments arranged by size:
3274 1634 683 56
BtsI GCAGTG_nn'
Cuts at: 2108 3163 3957 3977 2108
Size: 1055 794 20 3778
Fragments arranged by size:
3778 1055 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5647
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5612
DraI TTT'AAA
Cuts at: 2951 2951
Size: 5647
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4654 809
Size: 507 3338 1802
Fragments arranged by size:
3338 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2098 2832 2841
Size: 54 1425 50 275 18 268 734 9
Cuts at: 2841 3094 3983 5612 8
Size: 253 889 1629 43
Fragments arranged by size:
1629 1425 889 734 275 268 253 54
50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 3094 1537
Size: 275 1282 4090
Fragments arranged by size:
4090 1282 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2066 3577 1353
Size: 713 1511 3423
Fragments arranged by size:
3423 1511 713
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4234 4608 4754 387
Size: 1064 138 2645 374 146 1280
Fragments arranged by size:
2645 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 2205 2399 2519 2600 3710 4307
Size: 393 551 22 194 120 81 1110 597
Cuts at: 4307 5094 5362 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1110 787 597 551 393 268 194
120 81 22
EcoNI CCTnn'n_nnAGG
Cuts at: 2300 2501 2300
Size: 201 5446
EcoO109I rG'GnC_Cy
Cuts at: 2157 2209 2755 2157
Size: 52 546 5049
Fragments arranged by size:
5049 546 52
EcoRI G'AATT_C
Cuts at: 2956 3334 2956
Size: 378 5269
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2605 2605
Size: 5647
FspI TGC'GCA
Cuts at: 4153 4153
Size: 5647
HaeII r_GCGC'y
Cuts at: 1678 2015 2203 4794 1678
Size: 337 188 2591 2531
Fragments arranged by size:
2591 2531 337 188
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2621 2653 4288 4320 4362
Size: 32 135 32 1049 32 1635 32 42
Cuts at: 4362 4394 1373
Size: 32 2626
Fragments arranged by size:
2626 1635 1049 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2991 3237 669
Size: 988 281 1053 246 3079
Fragments arranged by size:
3079 1053 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5647
HpaI GTT'AAC
Cuts at: 3237 3237
Size: 5647
KasI G'GCGC_C
Cuts at: 2011 2199 2011
Size: 188 5459
KpnI G_GTAC'C
Cuts at: 2972 2972
Size: 5647
MfeI C'AATT_G
Cuts at: 3246 3246
Size: 5647
MluI A'CGCG_T
Cuts at: 5523 5523
Size: 5647
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2878 4761 4945 1305
Size: 1573 1883 184 2007
Fragments arranged by size:
2007 1883 1573 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2843 10
Size: 54 1425 343 1011 2814
Fragments arranged by size:
2814 1425 1011 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3605 3964 4123 518
Size: 648 2439 359 159 2042
Fragments arranged by size:
2439 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2248 2330 2554 2860 3063 3740 4888 5133
Size: 642 82 224 306 203 677 1148 245
Cuts at: 5133 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 642 306 245 224 203
82
NaeI GCC'GGC
Cuts at: 2098 2098
Size: 5647
NarI GG'CG_CC
Cuts at: 2012 2200 2012
Size: 188 5459
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4156
Fragments arranged by size:
4156 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5647
NgoMIV G'CCGG_C
Cuts at: 2096 2096
Size: 5647
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5647
NotI GC'GGCC_GC
Cuts at: 3094 3094
Size: 5647
NspI r_CATG'y
Cuts at: 1132 1229 3005 4550 1132
Size: 97 1776 1545 2229
Fragments arranged by size:
2229 1776 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5647
PciI A'CATG_T
Cuts at: 4546 4546
Size: 5647
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2251 1108
Size: 1143 4504
PfoI T'CCnGG_A
Cuts at: 2751 2751
Size: 5647
PmeI GTTT'AAAC
Cuts at: 2951 2951
Size: 5647
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3716 3748 5229 5261 3716
Size: 32 1481 32 4102
Fragments arranged by size:
4102 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2209 2755 2209
Size: 546 5101
PshAI GACnn'nnGTC
Cuts at: 1613 2449 1613
Size: 836 4811
PsiI TTA'TAA
Cuts at: 3217 3217
Size: 5647
PspOMI G'GGCC_C
Cuts at: 2156 2156
Size: 5647
PspXI vC'TCGA_Gb
Cuts at: 2447 2447
Size: 5647
PstI C_TGCA'G
Cuts at: 830 2999 830
Size: 2169 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5647
SalI G'TCGA_C
Cuts at: 1936 2989 1936
Size: 1053 4594
SbfI CC_TGCA'GG
Cuts at: 2999 2999
Size: 5647
ScaI AGT'ACT
Cuts at: 3895 5607 3895
Size: 1712 3935
SfcI C'TryA_G
Cuts at: 826 1045 2225 2995 4130 4811 5002 826
Size: 219 1180 770 1135 681 191 1471
Fragments arranged by size:
1471 1180 1135 770 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2840 2840
Size: 5647
SfoI GGC'GCC
Cuts at: 2013 2201 2013
Size: 188 5459
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5647
SmaI CCC'GGG
Cuts at: 1872 2974 1872
Size: 1102 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2447 3751 4652 4914 820
Size: 197 926 504 1304 901 262 1553
Fragments arranged by size:
1553 1304 926 901 504 262 197
SnaBI TAC'GTA
Cuts at: 493 3438 493
Size: 2945 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5647
SphI G_CATG'C
Cuts at: 1132 3005 1132
Size: 1873 3774
SspI AAT'ATT
Cuts at: 5 52 3571 5
Size: 47 3519 2081
Fragments arranged by size:
3519 2081 47
StuI AGG'CCT
Cuts at: 1517 2194 2588 1517
Size: 677 394 4576
Fragments arranged by size:
4576 677 394
StyI C'CwwG_G
Cuts at: 513 1065 2004 2682 3086 513
Size: 552 939 678 404 3074
Fragments arranged by size:
3074 939 678 552 404
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3674 3691 3844 4029 4474 325
Size: 1255 2094 17 153 185 445 1498
Fragments arranged by size:
2094 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3893 5605 96
Size: 274 80 33 51 1443 1916 1712 138
Fragments arranged by size:
1916 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5607 5639 5607
Size: 32 5615
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5647
XbaI T'CTAG_A
Cuts at: 2983 2983
Size: 5647
XhoI C'TCGA_G
Cuts at: 1943 2447 1943
Size: 504 5143
XmaI C'CCGG_G
Cuts at: 1870 2972 1870
Size: 1102 4545
XmnI GAAnn'nnTTC
Cuts at: 3776 3776
Size: 5647
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3455 276
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI SalI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FseI
FspAI NruI NsiI PacI PmlI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SrfI SwaI XcmI