Restriction map for
pFN21AE6624
(Circular) (Six-base) MAPSORT of: pFN21AE6624.seq Check: 125 from: 1 to: 5380
pFN21AE6624 5380 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2218 2218
Size: 5380
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3190 278
Size: 53 83 186 936 1654 2468
Fragments arranged by size:
2468 1654 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2723 1937
Size: 786 4594
Acc65I G'GTAC_C
Cuts at: 2701 2701
Size: 5380
AclI AA'CG_TT
Cuts at: 1563 3507 3880 1563
Size: 1944 373 3063
Fragments arranged by size:
3063 1944 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3443 4827 5095 1632
Size: 1811 1384 268 1917
Fragments arranged by size:
1917 1811 1384 268
AfeI AGC'GCT
Cuts at: 2198 2198
Size: 5380
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5183
AflIII A'CryG_T
Cuts at: 1203 4279 5256 1203
Size: 3076 977 1327
Fragments arranged by size:
3076 1327 977
AgeI A'CCGG_T
Cuts at: 4211 4211
Size: 5380
AhdI GACnn_n'nnGTC
Cuts at: 2134 4109 2134
Size: 1975 3405
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5380
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5380
AlwNI CAG_nnn'CTG
Cuts at: 2051 3123 4695 2051
Size: 1072 1572 2736
Fragments arranged by size:
2736 1572 1072
ApaLI G'TGCA_C
Cuts at: 3437 4593 3437
Size: 1156 4224
ApoI r'AATT_y
Cuts at: 1493 2411 2507 2689 2915 3067 5176 1493
Size: 918 96 182 226 152 2109 1697
Fragments arranged by size:
2109 1697 918 226 182 152 96
AseI AT'TA_AT
Cuts at: 160 1032 3934 160
Size: 872 2902 1606
Fragments arranged by size:
2902 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5380
AvaI C'yCGr_G
Cuts at: 1870 1943 2705 1870
Size: 73 762 4545
Fragments arranged by size:
4545 762 73
BamHI G'GATC_C
Cuts at: 2710 3077 2710
Size: 367 5013
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2543 2701 4156 618
Size: 325 217 627 756 158 1455 1842
Fragments arranged by size:
1842 1455 756 627 325 217 158
BanII G_rGCy'C
Cuts at: 1391 2699 1391
Size: 1308 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4409
BbvCI CC'TCA_GC
Cuts at: 2024 2024
Size: 5380
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3571 3605 1903
Size: 34 1634 34 3678
Fragments arranged by size:
3678 1634 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3272 4482 3272
Size: 1210 4170
BclI T'GATC_A
Cuts at: 1548 2604 1548
Size: 1056 4324
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3991 136
Size: 107 122 71 631 605 2319 1525
Fragments arranged by size:
2319 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5375 5375
Size: 5380
BlpI GC'TnA_GC
Cuts at: 2200 2797 2200
Size: 597 4783
Bme1580I G_kGCm'C
Cuts at: 1790 3441 4597 1790
Size: 1651 1156 2573
Fragments arranged by size:
2573 1651 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5380
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3111 4069 442
Size: 416 53 83 399 24 1694 958 1753
Fragments arranged by size:
1753 1694 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2108 1056
Size: 1052 4328
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2203 4040 1239
Size: 964 1837 2579
Fragments arranged by size:
2579 1837 964
Bpu10I CC'TnA_GC
Cuts at: 2024 2024
Size: 5380
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3505 4370 4668 3505
Size: 865 298 4217
Fragments arranged by size:
4217 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2014 4043 882
Size: 617 515 2029 2219
Fragments arranged by size:
2219 2029 617 515
BsaAI yAC'GTr
Cuts at: 493 1204 3171 493
Size: 711 1967 2702
Fragments arranged by size:
2702 1967 711
BsaBI GATnn'nnATC
Cuts at: 5374 5374
Size: 5380
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3187 3569 4204 275
Size: 53 83 186 936 1654 382 635 1451
Fragments arranged by size:
1654 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3813 4211 4485 4632 5043 3813
Size: 398 274 147 411 4150
Fragments arranged by size:
4150 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2586 2616 1178
Size: 30 165 30 1183 30 3942
Fragments arranged by size:
3942 1183 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5380
BseYI C'CCAG_C
Cuts at: 2380 2662 4583 5079 2380
Size: 282 1921 496 2681
Fragments arranged by size:
2681 1921 496 282
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2252 1163
Size: 573 516 4291
Fragments arranged by size:
4291 573 516
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2830 3591 4619 1540
Size: 275 186 829 761 1028 2301
Fragments arranged by size:
2301 1028 829 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3441 3526 4597 3441
Size: 85 1071 4224
Fragments arranged by size:
4224 1071 85
BsmI GAATG_Cn'
Cuts at: 2891 2984 2891
Size: 93 5287
BsmBI CGTCTCn'nnnn_
Cuts at: 2441 2441
Size: 5380
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2699 3441 3526 4597 1391
Size: 399 909 742 85 1071 2174
Fragments arranged by size:
2174 1071 909 742 399 85
BspEI T'CCGG_A
Cuts at: 5043 5043
Size: 5380
BspHI T'CATG_A
Cuts at: 2529 3269 4999 2529
Size: 740 1730 2910
Fragments arranged by size:
2910 1730 740
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2218 2426 2735 844
Size: 1374 208 309 3489
Fragments arranged by size:
3489 1374 309 208
BsrBI CCG'CTC
Cuts at: 1394 1823 3267 1394
Size: 429 1444 3507
Fragments arranged by size:
3507 1444 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3875 4049 65
Size: 1183 2627 174 1396
Fragments arranged by size:
2627 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4024 4211 1700
Size: 110 2214 187 2869
Fragments arranged by size:
2869 2214 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5380
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5380
BssSI C'ACGA_G
Cuts at: 3440 4452 3440
Size: 1012 4368
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5380
BstBI TT'CG_AA
Cuts at: 4184 4184
Size: 5380
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5380
BstXI CCAn_nnnn'nTGG
Cuts at: 2554 2554
Size: 5380
BstYI r'GATC_y
Cuts at: 1972 2710 3077 3462 3479 4931 5017 5029 5375
Size: 738 367 385 17 1452 86 12 346
Cuts at: 5375 1972
Size: 1977
Fragments arranged by size:
1977 1452 738 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2157 513
Size: 552 939 153 3736
Fragments arranged by size:
3736 939 552 153
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4641
Fragments arranged by size:
4641 683 56
BtsI GCAGTG_nn'
Cuts at: 2175 2896 3690 3710 2175
Size: 721 794 20 3845
Fragments arranged by size:
3845 794 721 20
ClaI AT'CG_AT
Cuts at: 1554 2097 1554
Size: 543 4837
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2098 2133 529
Size: 35 1534 35 3776
Fragments arranged by size:
3776 1534 35 35
DraI TTT'AAA
Cuts at: 2499 2684 2499
Size: 185 5195
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2075 4387 809
Size: 507 759 2312 1802
Fragments arranged by size:
2312 1802 759 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2827 3716 5345
Size: 54 1425 50 275 18 997 889 1629
Cuts at: 5345 8
Size: 43
Fragments arranged by size:
1629 1425 997 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2827 1537
Size: 275 1015 4090
Fragments arranged by size:
4090 1015 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3310 1353
Size: 1957 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3967 4341 4487 387
Size: 1064 138 2378 374 146 1280
Fragments arranged by size:
2378 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2203 3443 4040 4827 5095 1239
Size: 393 571 1240 597 787 268 1524
Fragments arranged by size:
1524 1240 787 597 571 393 268
EcoRI G'AATT_C
Cuts at: 2689 3067 2689
Size: 378 5002
FspI TGC'GCA
Cuts at: 3886 3886
Size: 5380
HaeII r_GCGC'y
Cuts at: 1678 2200 4527 1678
Size: 522 2327 2531
Fragments arranged by size:
2531 2327 522
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2120 2152 4021 4053 4095
Size: 32 135 32 548 32 1869 32 42
Cuts at: 4095 4127 1373
Size: 32 2626
Fragments arranged by size:
2626 1869 548 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2068 2724 2970 669
Size: 988 281 130 656 246 3079
Fragments arranged by size:
3079 988 656 281 246 130
HindIII A'AGCT_T
Cuts at: 748 2030 748
Size: 1282 4098
HpaI GTT'AAC
Cuts at: 2970 2970
Size: 5380
KpnI G_GTAC'C
Cuts at: 2705 2705
Size: 5380
MfeI C'AATT_G
Cuts at: 2979 2979
Size: 5380
MluI A'CGCG_T
Cuts at: 5256 5256
Size: 5380
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4494 4678 1305
Size: 3189 184 2007
Fragments arranged by size:
3189 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3558
Fragments arranged by size:
3558 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2552 3338 3697 3856 518
Size: 648 1386 786 359 159 2042
Fragments arranged by size:
2042 1386 786 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2462 2796 3473 4621 4866 1606
Size: 856 334 677 1148 245 2120
Fragments arranged by size:
2120 1148 856 677 334 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2157 513
Size: 552 939 153 3736
Fragments arranged by size:
3736 939 552 153
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5380
NheI G'CTAG_C
Cuts at: 1052 2104 1052
Size: 1052 4328
NotI GC'GGCC_GC
Cuts at: 2827 2827
Size: 5380
NspI r_CATG'y
Cuts at: 1132 1229 2360 2738 4283 1132
Size: 97 1131 378 1545 2229
Fragments arranged by size:
2229 1545 1131 378 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5380
PciI A'CATG_T
Cuts at: 4279 4279
Size: 5380
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5380
PmeI GTTT'AAAC
Cuts at: 2684 2684
Size: 5380
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3449 3481 4962 4994 3449
Size: 32 1481 32 3835
Fragments arranged by size:
3835 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5380
PsiI TTA'TAA
Cuts at: 2950 2950
Size: 5380
PstI C_TGCA'G
Cuts at: 830 2732 830
Size: 1902 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5380
PvuII CAG'CTG
Cuts at: 2462 2462
Size: 5380
SalI G'TCGA_C
Cuts at: 1936 2722 1936
Size: 786 4594
SbfI CC_TGCA'GG
Cuts at: 2732 2732
Size: 5380
ScaI AGT'ACT
Cuts at: 3628 5340 3628
Size: 1712 3668
SfcI C'TryA_G
Cuts at: 826 1045 2297 2728 3863 4544 4735 826
Size: 219 1252 431 1135 681 191 1471
Fragments arranged by size:
1471 1252 1135 681 431 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5380
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5380
SmaI CCC'GGG
Cuts at: 1872 2707 1872
Size: 835 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3484 4385 4647 820
Size: 197 926 1541 901 262 1553
Fragments arranged by size:
1553 1541 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3171 493
Size: 2678 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5380
SphI G_CATG'C
Cuts at: 1132 2738 1132
Size: 1606 3774
SspI AAT'ATT
Cuts at: 5 52 3304 5
Size: 47 3252 2081
Fragments arranged by size:
3252 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5380
StyI C'CwwG_G
Cuts at: 513 1065 2004 2157 2819 513
Size: 552 939 153 662 3074
Fragments arranged by size:
3074 939 662 552 153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3407 3424 3577 3762 4207 325
Size: 1255 1827 17 153 185 445 1498
Fragments arranged by size:
1827 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3626 5338 96
Size: 274 80 33 51 1443 1649 1712 138
Fragments arranged by size:
1712 1649 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2586 2618 5340 5372 2586
Size: 32 2722 32 2594
Fragments arranged by size:
2722 2594 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5380
XbaI T'CTAG_A
Cuts at: 2716 2716
Size: 5380
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5380
XmaI C'CCGG_G
Cuts at: 1870 2705 1870
Size: 835 4545
XmnI GAAnn'nnTTC
Cuts at: 2033 2508 3509 2033
Size: 475 1001 3904
Fragments arranged by size:
3904 1001 475
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3188 276
Size: 53 83 186 936 1654 2468
Fragments arranged by size:
2468 1654 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI