Restriction map for
pFN21AE6623
(Circular) (Six-base) MAPSORT of: pFN21AE6623.seq Check: 9451 from: 1 to: 5659
pFN21AE6623 5659 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3469 278
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2249 3002 1937
Size: 312 753 4594
Fragments arranged by size:
4594 753 312
Acc65I G'GTAC_C
Cuts at: 2980 2980
Size: 5659
AclI AA'CG_TT
Cuts at: 1563 3786 4159 1563
Size: 2223 373 3063
Fragments arranged by size:
3063 2223 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2106 2127 3722 5106 5374 1632
Size: 474 21 1595 1384 268 1917
Fragments arranged by size:
1917 1595 1384 474 268 21
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5462
AflIII A'CryG_T
Cuts at: 1203 2477 2479 2682 4558 5535 1203
Size: 1274 2 203 1876 977 1327
Fragments arranged by size:
1876 1327 1274 977 203 2
AgeI A'CCGG_T
Cuts at: 4490 4490
Size: 5659
AhdI GACnn_n'nnGTC
Cuts at: 4388 4388
Size: 5659
AleI CACnn'nnGTG
Cuts at: 1166 2480 1166
Size: 1314 4345
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2642 1184
Size: 1458 4201
AlwNI CAG_nnn'CTG
Cuts at: 2386 2503 2548 3402 4974 2386
Size: 117 45 854 1572 3071
Fragments arranged by size:
3071 1572 854 117 45
ApaI G_GGCC'C
Cuts at: 2614 2614
Size: 5659
ApaLI G'TGCA_C
Cuts at: 3716 4872 3716
Size: 1156 4503
ApoI r'AATT_y
Cuts at: 1493 2968 3194 3346 5455 1493
Size: 1475 226 152 2109 1697
Fragments arranged by size:
2109 1697 1475 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4213 160
Size: 872 3181 1606
Fragments arranged by size:
3181 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
AvaI C'yCGr_G
Cuts at: 1870 1943 2607 2984 1870
Size: 73 664 377 4545
Fragments arranged by size:
4545 664 377 73
BamHI G'GATC_C
Cuts at: 2989 3356 2989
Size: 367 5292
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2101 2980 4435 618
Size: 325 217 627 314 879 1455 1842
Fragments arranged by size:
1842 1455 879 627 325 314 217
BanII G_rGCy'C
Cuts at: 1391 2380 2614 2978 1391
Size: 989 234 364 4072
Fragments arranged by size:
4072 989 364 234
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2133 2220 2540 928
Size: 971 234 87 320 4047
Fragments arranged by size:
4047 971 320 234 87
BbvCI CC'TCA_GC
Cuts at: 2615 2615
Size: 5659
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3850 3884 1903
Size: 34 1913 34 3678
Fragments arranged by size:
3678 1913 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3551 4761 3551
Size: 1210 4449
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5659
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2043 2744
Size: 107 122 71 631 605 337 34 701
Cuts at: 2744 4270 136
Size: 1526 1525
Fragments arranged by size:
1526 1525 701 631 605 337 122 107
71 34
BglII A'GATC_T
Cuts at: 5654 5654
Size: 5659
BlpI GC'TnA_GC
Cuts at: 3076 3076
Size: 5659
Bme1580I G_kGCm'C
Cuts at: 1790 2340 2614 3720 4876 1790
Size: 550 274 1106 1156 2573
Fragments arranged by size:
2573 1156 1106 550 274
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5659
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2374 2452 3390
Size: 416 53 83 399 24 957 78 938
Cuts at: 3390 4348 442
Size: 958 1753
Fragments arranged by size:
1753 958 957 938 416 399 83 78
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5659
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2068 2380 2778 4319 1239
Size: 829 312 398 1541 2579
Fragments arranged by size:
2579 1541 829 398 312
Bpu10I CC'TnA_GC
Cuts at: 2135 2615 2654 2135
Size: 480 39 5140
Fragments arranged by size:
5140 480 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2348 2912 3784 4649 4947 2348
Size: 564 872 865 298 3060
Fragments arranged by size:
3060 872 865 564 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2396 4322 882
Size: 617 897 1926 2219
Fragments arranged by size:
2219 1926 897 617
BsaAI yAC'GTr
Cuts at: 493 1204 2480 3450 493
Size: 711 1276 970 2702
Fragments arranged by size:
2702 1276 970 711
BsaBI GATnn'nnATC
Cuts at: 5653 5653
Size: 5659
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3466 3848 4483 275
Size: 53 83 186 936 1933 382 635 1451
Fragments arranged by size:
1933 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4092 4490 4764 4911 5322 4092
Size: 398 274 147 411 4429
Fragments arranged by size:
4429 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5434
Fragments arranged by size:
5434 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2282 2285 2288 2306 2507 2779 1186
Size: 1096 3 3 18 201 272 4066
Fragments arranged by size:
4066 1096 272 201 18 3 3
BseYI C'CCAG_C
Cuts at: 2696 4862 5358 2696
Size: 2166 496 2997
Fragments arranged by size:
2997 2166 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2421 2934 1163
Size: 573 685 513 3888
Fragments arranged by size:
3888 685 573 513
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3109 3870 4898 1540
Size: 275 186 1108 761 1028 2301
Fragments arranged by size:
2301 1108 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2601 3720 3805 4876 2601
Size: 1119 85 1071 3384
Fragments arranged by size:
3384 1119 1071 85
BsmI GAATG_Cn'
Cuts at: 2188 2583 3170 3263 2188
Size: 395 587 93 4584
Fragments arranged by size:
4584 587 395 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2340 2380 2601 2614 2978 3720 3805
Size: 399 550 40 221 13 364 742 85
Cuts at: 3805 4876 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 550 399 364 221 85
40 13
BspEI T'CCGG_A
Cuts at: 5322 5322
Size: 5659
BspHI T'CATG_A
Cuts at: 3548 5278 3548
Size: 1730 3929
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2860 3014 844
Size: 2016 154 3489
Fragments arranged by size:
3489 2016 154
BsrBI CCG'CTC
Cuts at: 1394 1823 3546 1394
Size: 429 1723 3507
Fragments arranged by size:
3507 1723 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2850 4154 4328 65
Size: 1183 1602 1304 174 1396
Fragments arranged by size:
1602 1396 1304 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4303 4490 1700
Size: 110 2493 187 2869
Fragments arranged by size:
2869 2493 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5659
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5659
BssSI C'ACGA_G
Cuts at: 3719 4731 3719
Size: 1012 4647
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5659
BstBI TT'CG_AA
Cuts at: 4463 4463
Size: 5659
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5659
BstXI CCAn_nnnn'nTGG
Cuts at: 2674 2674
Size: 5659
BstYI r'GATC_y
Cuts at: 1972 2989 3356 3741 3758 5210 5296 5308 5654
Size: 1017 367 385 17 1452 86 12 346
Cuts at: 5654 1972
Size: 1977
Fragments arranged by size:
1977 1452 1017 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2250 2250
Size: 5659
Bsu36I CC'TnA_GG
Cuts at: 2549 2549
Size: 5659
BtgI C'CryG_G
Cuts at: 513 1065 2004 2076 2344 513
Size: 552 939 72 268 3828
Fragments arranged by size:
3828 939 552 268 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2080 487
Size: 683 56 854 4066
Fragments arranged by size:
4066 854 683 56
BtsI GCAGTG_nn'
Cuts at: 2437 2565 2859 2925 3175 3969 3989 2437
Size: 128 294 66 250 794 20 4107
Fragments arranged by size:
4107 794 294 250 128 66 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5659
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5624
DraI TTT'AAA
Cuts at: 2963 2963
Size: 5659
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4666 809
Size: 507 3350 1802
Fragments arranged by size:
3350 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2035 3106 3995
Size: 54 1425 50 275 18 205 1071 889
Cuts at: 3995 5624 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1071 889 275 205 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3106 1537
Size: 275 1294 4090
Fragments arranged by size:
4090 1294 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2484 2522 3589 1353
Size: 1131 38 1067 3423
Fragments arranged by size:
3423 1131 1067 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2537 4246 4620 4766 387
Size: 1064 138 948 1709 374 146 1280
Fragments arranged by size:
1709 1280 1064 948 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2068 2106 2127 2380 2778 3722 4319
Size: 393 436 38 21 253 398 944 597
Cuts at: 4319 5106 5374 1239
Size: 787 268 1524
Fragments arranged by size:
1524 944 787 597 436 398 393 268
253 38 21
EcoO109I rG'GnC_Cy
Cuts at: 2611 2611
Size: 5659
EcoRI G'AATT_C
Cuts at: 2968 3346 2968
Size: 378 5281
FspI TGC'GCA
Cuts at: 4165 4165
Size: 5659
HaeII r_GCGC'y
Cuts at: 1678 2818 4806 1678
Size: 1140 1988 2531
Fragments arranged by size:
2531 1988 1140
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2210 2242 4300 4332 4374
Size: 32 135 32 638 32 2058 32 42
Cuts at: 4374 4406 1373
Size: 32 2626
Fragments arranged by size:
2626 2058 638 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3003 3249 669
Size: 988 281 1065 246 3079
Fragments arranged by size:
3079 1065 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5659
HpaI GTT'AAC
Cuts at: 3249 3249
Size: 5659
KpnI G_GTAC'C
Cuts at: 2984 2984
Size: 5659
MfeI C'AATT_G
Cuts at: 3258 3258
Size: 5659
MluI A'CGCG_T
Cuts at: 5535 5535
Size: 5659
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4773 4957 1305
Size: 3468 184 2007
Fragments arranged by size:
3468 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3837
Fragments arranged by size:
3837 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2480 2624 2669 3617 3976 4135 518
Size: 648 1314 144 45 948 359 159 2042
Fragments arranged by size:
2042 1314 948 648 359 159 144 45
MspA1I CmG'CkG
Cuts at: 1606 2062 2126 3075 3752 4900 5145 1606
Size: 456 64 949 677 1148 245 2120
Fragments arranged by size:
2120 1148 949 677 456 245 64
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4168
Fragments arranged by size:
4168 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5659
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5659
NotI GC'GGCC_GC
Cuts at: 3106 3106
Size: 5659
NspI r_CATG'y
Cuts at: 1132 1229 2416 2686 3017 4562 1132
Size: 97 1187 270 331 1545 2229
Fragments arranged by size:
2229 1545 1187 331 270 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5659
PciI A'CATG_T
Cuts at: 2682 4558 2682
Size: 1876 3783
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2386 1108
Size: 1278 4381
PfoI T'CCnGG_A
Cuts at: 2820 2820
Size: 5659
PmeI GTTT'AAAC
Cuts at: 2963 2963
Size: 5659
PmlI CAC'GTG
Cuts at: 2480 2480
Size: 5659
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3728 3760 5241 5273 3728
Size: 32 1481 32 4114
Fragments arranged by size:
4114 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5659
PsiI TTA'TAA
Cuts at: 3229 3229
Size: 5659
PspOMI G'GGCC_C
Cuts at: 2610 2610
Size: 5659
PstI C_TGCA'G
Cuts at: 830 2061 3011 830
Size: 1231 950 3478
Fragments arranged by size:
3478 1231 950
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5659
PvuII CAG'CTG
Cuts at: 2062 2126 2062
Size: 64 5595
SalI G'TCGA_C
Cuts at: 1936 3001 1936
Size: 1065 4594
SbfI CC_TGCA'GG
Cuts at: 3011 3011
Size: 5659
ScaI AGT'ACT
Cuts at: 3907 5619 3907
Size: 1712 3947
SfcI C'TryA_G
Cuts at: 826 1045 2057 3007 4142 4823 5014 826
Size: 219 1012 950 1135 681 191 1471
Fragments arranged by size:
1471 1135 1012 950 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5659
SmaI CCC'GGG
Cuts at: 1872 2609 2986 1872
Size: 737 377 4545
Fragments arranged by size:
4545 737 377
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2327 2927 3763 4664 4926 820
Size: 197 926 384 600 836 901 262 1553
Fragments arranged by size:
1553 926 901 836 600 384 262 197
SnaBI TAC'GTA
Cuts at: 493 3450 493
Size: 2957 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5659
SphI G_CATG'C
Cuts at: 1132 3017 1132
Size: 1885 3774
SspI AAT'ATT
Cuts at: 5 52 3583 5
Size: 47 3531 2081
Fragments arranged by size:
3531 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5659
StyI C'CwwG_G
Cuts at: 513 1065 2004 3098 513
Size: 552 939 1094 3074
Fragments arranged by size:
3074 1094 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2900 3686 3703 3856 4041 4486 325
Size: 1255 1320 786 17 153 185 445 1498
Fragments arranged by size:
1498 1320 1255 786 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3905 5617 96
Size: 274 80 33 51 1443 1928 1712 138
Fragments arranged by size:
1928 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2669 2701 5619 5651 2669
Size: 32 2918 32 2677
Fragments arranged by size:
2918 2677 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5659
XbaI T'CTAG_A
Cuts at: 2995 2995
Size: 5659
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5659
XmaI C'CCGG_G
Cuts at: 1870 2607 2984 1870
Size: 737 377 4545
Fragments arranged by size:
4545 737 377
XmnI GAAnn'nnTTC
Cuts at: 3788 3788
Size: 5659
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3467 276
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PpuMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI