Restriction map for
pFN21AE6620
(Circular) (Six-base) MAPSORT of: pFN21AE6620.seq Check: 7104 from: 1 to: 5686
pFN21AE6620 5686 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3496 278
Size: 53 83 186 936 1960 2468
Fragments arranged by size:
2468 1960 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3029 1937
Size: 1092 4594
Acc65I G'GTAC_C
Cuts at: 3007 3007
Size: 5686
AclI AA'CG_TT
Cuts at: 1563 3813 4186 1563
Size: 2250 373 3063
Fragments arranged by size:
3063 2250 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2888 3749 5133 5401 1632
Size: 1256 861 1384 268 1917
Fragments arranged by size:
1917 1384 1256 861 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5489
AflIII A'CryG_T
Cuts at: 1203 4585 5562 1203
Size: 3382 977 1327
Fragments arranged by size:
3382 1327 977
AgeI A'CCGG_T
Cuts at: 4517 4517
Size: 5686
AhdI GACnn_n'nnGTC
Cuts at: 4415 4415
Size: 5686
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5686
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5686
AlwNI CAG_nnn'CTG
Cuts at: 2806 2929 3429 5001 2806
Size: 123 500 1572 3491
Fragments arranged by size:
3491 1572 500 123
ApaLI G'TGCA_C
Cuts at: 3743 4899 3743
Size: 1156 4530
ApoI r'AATT_y
Cuts at: 1493 2995 3221 3373 5482 1493
Size: 1502 226 152 2109 1697
Fragments arranged by size:
2109 1697 1502 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4240 160
Size: 872 3208 1606
Fragments arranged by size:
3208 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5686
AvaI C'yCGr_G
Cuts at: 1870 1943 2273 3011 1870
Size: 73 330 738 4545
Fragments arranged by size:
4545 738 330 73
BamHI G'GATC_C
Cuts at: 2856 3016 3383 2856
Size: 160 367 5159
Fragments arranged by size:
5159 367 160
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2198 3007 4462 618
Size: 325 217 627 411 809 1455 1842
Fragments arranged by size:
1842 1455 809 627 411 325 217
BanII G_rGCy'C
Cuts at: 1391 2234 2749 2936 3005 1391
Size: 843 515 187 69 4072
Fragments arranged by size:
4072 843 515 187 69
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2019 928
Size: 971 120 4595
Fragments arranged by size:
4595 971 120
BbvCI CC'TCA_GC
Cuts at: 2940 2940
Size: 5686
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2757 2791 3877 3911 1903
Size: 34 50 34 736 34 1086 34 3678
Fragments arranged by size:
3678 1086 736 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3578 4788 3578
Size: 1210 4476
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5686
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4297 136
Size: 107 122 71 631 605 2625 1525
Fragments arranged by size:
2625 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5681 5681
Size: 5686
BlpI GC'TnA_GC
Cuts at: 2045 2930 3103 2045
Size: 885 173 4628
Fragments arranged by size:
4628 885 173
Bme1580I G_kGCm'C
Cuts at: 1790 2201 3747 4903 1790
Size: 411 1546 1156 2573
Fragments arranged by size:
2573 1546 1156 411
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5686
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2204 3417 4375
Size: 416 53 83 399 24 787 1213 958
Cuts at: 4375 442
Size: 1753
Fragments arranged by size:
1753 1213 958 787 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2327 1056
Size: 1271 4415
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2195 2783 4346 1239
Size: 956 588 1563 2579
Fragments arranged by size:
2579 1563 956 588
Bpu10I CC'TnA_GC
Cuts at: 2748 2940 2748
Size: 192 5494
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2334 3811 4676 4974 2334
Size: 1477 865 298 3046
Fragments arranged by size:
3046 1477 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4349 882
Size: 617 2850 2219
Fragments arranged by size:
2850 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3477 493
Size: 711 2273 2702
Fragments arranged by size:
2702 2273 711
BsaBI GATnn'nnATC
Cuts at: 5680 5680
Size: 5686
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2718 3493 3875 4510
Size: 53 83 186 936 1185 775 382 635
Cuts at: 4510 275
Size: 1451
Fragments arranged by size:
1451 1185 936 775 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2333 4119 4517 4791 4938 5349 2333
Size: 1786 398 274 147 411 2670
Fragments arranged by size:
2670 1786 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2756 2786 1178
Size: 30 165 30 1353 30 4078
Fragments arranged by size:
4078 1353 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2192 2957 2960 1186
Size: 1006 765 3 3912
Fragments arranged by size:
3912 1006 765 3
BseYI C'CCAG_C
Cuts at: 2008 2515 2565 4889 5385 2008
Size: 507 50 2324 496 2309
Fragments arranged by size:
2324 2309 507 496 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2171 2761 1163
Size: 573 435 590 4088
Fragments arranged by size:
4088 590 573 435
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3136 3897 4925 1540
Size: 275 186 1135 761 1028 2301
Fragments arranged by size:
2301 1135 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3747 3832 4903 3747
Size: 85 1071 4530
Fragments arranged by size:
4530 1071 85
BsmI GAATG_Cn'
Cuts at: 3197 3290 3197
Size: 93 5593
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2201 2234 2749 2936 3005 3747 3832
Size: 399 411 33 515 187 69 742 85
Cuts at: 3832 4903 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 515 411 399 187 85
69 33
BspEI T'CCGG_A
Cuts at: 2333 5349 2333
Size: 3016 2670
BspHI T'CATG_A
Cuts at: 3575 5305 3575
Size: 1730 3956
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2544 3041 844
Size: 1700 497 3489
Fragments arranged by size:
3489 1700 497
BsrBI CCG'CTC
Cuts at: 1394 1823 3573 1394
Size: 429 1750 3507
Fragments arranged by size:
3507 1750 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2106 2792 4181 4355 65
Size: 1183 858 686 1389 174 1396
Fragments arranged by size:
1396 1389 1183 858 686 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4330 4517 1700
Size: 110 2520 187 2869
Fragments arranged by size:
2869 2520 187 110
BsrGI T'GTAC_A
Cuts at: 96 2505 96
Size: 2409 3277
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5686
BssSI C'ACGA_G
Cuts at: 3746 4758 3746
Size: 1012 4674
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5686
BstBI TT'CG_AA
Cuts at: 4490 4490
Size: 5686
BstEII G'GTnAC_C
Cuts at: 1184 2410 2839 1184
Size: 1226 429 4031
Fragments arranged by size:
4031 1226 429
BstXI CCAn_nnnn'nTGG
Cuts at: 2068 2740 2068
Size: 672 5014
BstYI r'GATC_y
Cuts at: 1972 2856 3016 3383 3768 3785 5237 5323 5335
Size: 884 160 367 385 17 1452 86 12
Cuts at: 5335 5681 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 884 385 367 346 160 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2083 2083
Size: 5686
BtgI C'CryG_G
Cuts at: 513 1065 2403 513
Size: 552 1338 3796
Fragments arranged by size:
3796 1338 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4947
Fragments arranged by size:
4947 683 56
BtsI GCAGTG_nn'
Cuts at: 2142 2148 2269 2449 2556 2951 3202 3996 4016
Size: 6 121 180 107 395 251 794 20
Cuts at: 4016 2142
Size: 3812
Fragments arranged by size:
3812 794 395 251 180 121 107 20
6
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5686
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2478 2513 529
Size: 35 1914 35 3702
Fragments arranged by size:
3702 1914 35 35
DraI TTT'AAA
Cuts at: 2990 2990
Size: 5686
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4693 809
Size: 507 3377 1802
Fragments arranged by size:
3377 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2400 2431 2688
Size: 54 1425 50 275 18 570 31 257
Cuts at: 2688 2730 3133 4022 5651 8
Size: 42 403 889 1629 43
Fragments arranged by size:
1629 1425 889 570 403 275 257 54
50 43 42 31 18
EagI C'GGCC_G
Cuts at: 1537 1812 3133 1537
Size: 275 1321 4090
Fragments arranged by size:
4090 1321 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3616 1353
Size: 2263 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4273 4647 4793 387
Size: 1064 138 2684 374 146 1280
Fragments arranged by size:
2684 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2195 2783 2888 3749 4346 5133 5401
Size: 393 563 588 105 861 597 787 268
Cuts at: 5401 1239
Size: 1524
Fragments arranged by size:
1524 861 787 597 588 563 393 268
105
EcoO109I rG'GnC_Cy
Cuts at: 2012 2903 2012
Size: 891 4795
EcoRI G'AATT_C
Cuts at: 2995 3373 2995
Size: 378 5308
FspI TGC'GCA
Cuts at: 2957 4192 2957
Size: 1235 4451
HaeII r_GCGC'y
Cuts at: 1678 2272 2851 4833 1678
Size: 594 579 1982 2531
Fragments arranged by size:
2531 1982 594 579
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4327 4359 4401 4433 1373
Size: 32 135 32 2755 32 42 32 2626
Fragments arranged by size:
2755 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3030 3276 669
Size: 988 281 1092 246 3079
Fragments arranged by size:
3079 1092 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5686
HpaI GTT'AAC
Cuts at: 3276 3276
Size: 5686
KpnI G_GTAC'C
Cuts at: 3011 3011
Size: 5686
MfeI C'AATT_G
Cuts at: 3285 3285
Size: 5686
MluI A'CGCG_T
Cuts at: 5562 5562
Size: 5686
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4800 4984 1305
Size: 3495 184 2007
Fragments arranged by size:
3495 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2690 2732 10
Size: 54 1425 343 858 42 2964
Fragments arranged by size:
2964 1425 858 343 54 42
MslI CAynn'nnrTG
Cuts at: 518 1166 2408 3644 4003 4162 518
Size: 648 1242 1236 359 159 2042
Fragments arranged by size:
2042 1242 1236 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2598 2761 2939 3102 3779 4927 5172 1606
Size: 992 163 178 163 677 1148 245 2120
Fragments arranged by size:
2120 1148 992 677 245 178 163 163
NcoI C'CATG_G
Cuts at: 513 1065 2403 513
Size: 552 1338 3796
Fragments arranged by size:
3796 1338 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5686
NheI G'CTAG_C
Cuts at: 1052 2323 1052
Size: 1271 4415
NotI GC'GGCC_GC
Cuts at: 3133 3133
Size: 5686
NspI r_CATG'y
Cuts at: 1132 1229 3044 4589 1132
Size: 97 1815 1545 2229
Fragments arranged by size:
2229 1815 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2861 1348
Size: 1513 4173
PciI A'CATG_T
Cuts at: 4585 4585
Size: 5686
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5686
PfoI T'CCnGG_A
Cuts at: 2060 2060
Size: 5686
PmeI GTTT'AAAC
Cuts at: 2990 2990
Size: 5686
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3755 3787 5268 5300 3755
Size: 32 1481 32 4141
Fragments arranged by size:
4141 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2012 2012
Size: 5686
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5686
PsiI TTA'TAA
Cuts at: 3256 3256
Size: 5686
PspXI vC'TCGA_Gb
Cuts at: 2273 2273
Size: 5686
PstI C_TGCA'G
Cuts at: 830 2155 2811 3038 830
Size: 1325 656 227 3478
Fragments arranged by size:
3478 1325 656 227
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5686
PvuII CAG'CTG
Cuts at: 2761 2761
Size: 5686
SalI G'TCGA_C
Cuts at: 1936 3028 1936
Size: 1092 4594
SanDI GG'GwC_CC
Cuts at: 2012 2012
Size: 5686
SbfI CC_TGCA'GG
Cuts at: 3038 3038
Size: 5686
ScaI AGT'ACT
Cuts at: 3934 5646 3934
Size: 1712 3974
SfcI C'TryA_G
Cuts at: 826 1045 2151 2807 3034 4169 4850 5041 826
Size: 219 1106 656 227 1135 681 191 1471
Fragments arranged by size:
1471 1135 1106 681 656 227 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5686
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5686
SmaI CCC'GGG
Cuts at: 1872 3013 1872
Size: 1141 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2273 2349 3790 4691 4953 820
Size: 197 926 330 76 1441 901 262 1553
Fragments arranged by size:
1553 1441 926 901 330 262 197 76
SnaBI TAC'GTA
Cuts at: 493 3477 493
Size: 2984 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5686
SphI G_CATG'C
Cuts at: 1132 3044 1132
Size: 1912 3774
SspI AAT'ATT
Cuts at: 5 52 2578 3610 5
Size: 47 2526 1032 2081
Fragments arranged by size:
2526 2081 1032 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5686
StyI C'CwwG_G
Cuts at: 513 1065 2403 2469 2700 3125 513
Size: 552 1338 66 231 425 3074
Fragments arranged by size:
3074 1338 552 425 231 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3713 3730 3883 4068 4513 325
Size: 1255 2133 17 153 185 445 1498
Fragments arranged by size:
2133 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2505 3932 5644
Size: 274 80 33 51 1443 528 1427 1712
Cuts at: 5644 96
Size: 138
Fragments arranged by size:
1712 1443 1427 528 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2756 2788 5646 5678 2756
Size: 32 2858 32 2764
Fragments arranged by size:
2858 2764 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5686
XbaI T'CTAG_A
Cuts at: 3022 3022
Size: 5686
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2155 2234 2527 2698 2155
Size: 79 293 171 5143
Fragments arranged by size:
5143 293 171 79
XhoI C'TCGA_G
Cuts at: 1943 2273 1943
Size: 330 5356
XmaI C'CCGG_G
Cuts at: 1870 3011 1870
Size: 1141 4545
XmnI GAAnn'nnTTC
Cuts at: 3815 3815
Size: 5686
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3494 276
Size: 53 83 186 936 1960 2468
Fragments arranged by size:
2468 1960 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI PvuII SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PsrI RsrII SacI
SacII SapI SexAI SfiI SfoI SrfI SwaI