Restriction map for
pFN21AE6617
(Circular) (Six-base) MAPSORT of: pFN21AE6617.seq Check: 7494 from: 1 to: 5713
pFN21AE6617 5713 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3523 278
Size: 53 83 186 936 1987 2468
Fragments arranged by size:
2468 1987 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3056 1937
Size: 1119 4594
Acc65I G'GTAC_C
Cuts at: 3034 3034
Size: 5713
AclI AA'CG_TT
Cuts at: 1563 3840 4213 1563
Size: 2277 373 3063
Fragments arranged by size:
3063 2277 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2083 3776 5160 5428 1632
Size: 451 1693 1384 268 1917
Fragments arranged by size:
1917 1693 1384 451 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5516
AflIII A'CryG_T
Cuts at: 1203 4612 5589 1203
Size: 3409 977 1327
Fragments arranged by size:
3409 1327 977
AgeI A'CCGG_T
Cuts at: 4544 4544
Size: 5713
AhdI GACnn_n'nnGTC
Cuts at: 4442 4442
Size: 5713
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5713
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5713
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2534 2566 2534
Size: 32 5681
AlwNI CAG_nnn'CTG
Cuts at: 2248 2568 2920 3456 5028 2248
Size: 320 352 536 1572 2933
Fragments arranged by size:
2933 1572 536 352 320
ApaLI G'TGCA_C
Cuts at: 3770 4926 3770
Size: 1156 4557
ApoI r'AATT_y
Cuts at: 1493 3022 3248 3400 5509 1493
Size: 1529 226 152 2109 1697
Fragments arranged by size:
2109 1697 1529 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4267 160
Size: 872 3235 1606
Fragments arranged by size:
3235 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5713
AvaI C'yCGr_G
Cuts at: 1870 1943 3038 1870
Size: 73 1095 4545
Fragments arranged by size:
4545 1095 73
BamHI G'GATC_C
Cuts at: 2193 2898 3043 3410 2193
Size: 705 145 367 4496
Fragments arranged by size:
4496 705 367 145
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2829 3034 4489 618
Size: 325 217 627 1042 205 1455 1842
Fragments arranged by size:
1842 1455 1042 627 325 217 205
BanII G_rGCy'C
Cuts at: 1391 2578 3032 1391
Size: 1187 454 4072
Fragments arranged by size:
4072 1187 454
BbeI G_GCGC'C
Cuts at: 2833 2833
Size: 5713
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4742
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3904 3938 1903
Size: 34 1967 34 3678
Fragments arranged by size:
3678 1967 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3605 4815 3605
Size: 1210 4503
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5713
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2069 2983 4324
Size: 107 122 71 631 605 397 914 1341
Cuts at: 4324 136
Size: 1525
Fragments arranged by size:
1525 1341 914 631 605 397 122 107
71
BglII A'GATC_T
Cuts at: 5708 5708
Size: 5713
BlpI GC'TnA_GC
Cuts at: 2261 2702 3130 2261
Size: 441 428 4844
Fragments arranged by size:
4844 441 428
Bme1580I G_kGCm'C
Cuts at: 1790 3774 4930 1790
Size: 1984 1156 2573
Fragments arranged by size:
2573 1984 1156
BmgBI CAC'GTC
Cuts at: 1318 2764 2932 1318
Size: 1446 168 4099
Fragments arranged by size:
4099 1446 168
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2578 3444 4402
Size: 416 53 83 399 24 1161 866 958
Cuts at: 4402 442
Size: 1753
Fragments arranged by size:
1753 1161 958 866 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5713
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2099 2291 2393 2522 2545 2897 4373 1239
Size: 860 192 102 129 23 352 1476 2579
Fragments arranged by size:
2579 1476 860 352 192 129 102 23
Bpu10I CC'TnA_GC
Cuts at: 2774 2774
Size: 5713
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3838 4703 5001 3838
Size: 865 298 4550
Fragments arranged by size:
4550 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2438 2648 4376 882
Size: 617 939 210 1728 2219
Fragments arranged by size:
2219 1728 939 617 210
BsaAI yAC'GTr
Cuts at: 493 1204 2047 2503 3504 493
Size: 711 843 456 1001 2702
Fragments arranged by size:
2702 1001 843 711 456
BsaBI GATnn'nnATC
Cuts at: 5707 5707
Size: 5713
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2830 3520 3902 4537
Size: 53 83 186 936 1297 690 382 635
Cuts at: 4537 275
Size: 1451
Fragments arranged by size:
1451 1297 936 690 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4146 4544 4818 4965 5376 4146
Size: 398 274 147 411 4483
Fragments arranged by size:
4483 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2870 2900 1178
Size: 30 165 30 1467 30 3991
Fragments arranged by size:
3991 1467 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2630 1186
Size: 1444 4269
BseYI C'CCAG_C
Cuts at: 2073 2238 2580 4916 5412 2073
Size: 165 342 2336 496 2374
Fragments arranged by size:
2374 2336 496 342 165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2540 2678 1163
Size: 573 804 138 4198
Fragments arranged by size:
4198 804 573 138
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2064 2687 2881 3163 3924 4952
Size: 275 186 63 623 194 282 761 1028
Cuts at: 4952 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 623 282 275 194 186
63
BsiHKAI G_wGCw'C
Cuts at: 2578 3774 3859 4930 2578
Size: 1196 85 1071 3361
Fragments arranged by size:
3361 1196 1071 85
BsmI GAATG_Cn'
Cuts at: 3224 3317 3224
Size: 93 5620
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2578 3032 3774 3859 4930 1391
Size: 399 788 454 742 85 1071 2174
Fragments arranged by size:
2174 1071 788 742 454 399 85
BspEI T'CCGG_A
Cuts at: 5376 5376
Size: 5713
BspHI T'CATG_A
Cuts at: 3602 5332 3602
Size: 1730 3983
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2105 2115 2178 2865 3068 844
Size: 1261 10 63 687 203 3489
Fragments arranged by size:
3489 1261 687 203 63 10
BsrBI CCG'CTC
Cuts at: 1394 1823 2815 2962 3600 1394
Size: 429 992 147 638 3507
Fragments arranged by size:
3507 992 638 429 147
BsrDI GCAATG_nn'
Cuts at: 65 1248 2676 4208 4382 65
Size: 1183 1428 1532 174 1396
Fragments arranged by size:
1532 1428 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4357 4544 1700
Size: 110 2547 187 2869
Fragments arranged by size:
2869 2547 187 110
BsrGI T'GTAC_A
Cuts at: 96 2673 96
Size: 2577 3136
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5713
BssSI C'ACGA_G
Cuts at: 2135 2612 3773 4785 2135
Size: 477 1161 1012 3063
Fragments arranged by size:
3063 1161 1012 477
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2248 794
Size: 1454 4259
BstBI TT'CG_AA
Cuts at: 4517 4517
Size: 5713
BstEII G'GTnAC_C
Cuts at: 1184 2468 1184
Size: 1284 4429
BstXI CCAn_nnnn'nTGG
Cuts at: 2480 2641 2936 2480
Size: 161 295 5257
Fragments arranged by size:
5257 295 161
BstYI r'GATC_y
Cuts at: 1972 2193 2898 3043 3410 3795 3812 5264 5350
Size: 221 705 145 367 385 17 1452 86
Cuts at: 5350 5362 5708 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 705 385 367 346 221 145
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2067 513
Size: 552 939 63 4159
Fragments arranged by size:
4159 939 552 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2326 2644 487
Size: 683 56 1100 318 3556
Fragments arranged by size:
3556 1100 683 318 56
BtsI GCAGTG_nn'
Cuts at: 2094 3229 4023 4043 2094
Size: 1135 794 20 3764
Fragments arranged by size:
3764 1135 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5713
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2505 2540 529
Size: 35 1941 35 3702
Fragments arranged by size:
3702 1941 35 35
DraI TTT'AAA
Cuts at: 3017 3017
Size: 5713
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4720 809
Size: 507 3404 1802
Fragments arranged by size:
3404 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2061 2358 2684
Size: 54 1425 50 275 18 231 297 326
Cuts at: 2684 2922 3160 4049 5678 8
Size: 238 238 889 1629 43
Fragments arranged by size:
1629 1425 889 326 297 275 238 238
231 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2061 2684 3160 1537
Size: 275 249 623 476 4090
Fragments arranged by size:
4090 623 476 275 249
EarI CTCTTCn'nnn_
Cuts at: 1353 2909 3643 1353
Size: 1556 734 3423
Fragments arranged by size:
3423 1556 734
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4300 4674 4820 387
Size: 1064 138 2711 374 146 1280
Fragments arranged by size:
2711 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2576 2576
Size: 5713
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2083 2099 2291 2393 2522 2545 2897
Size: 393 451 16 192 102 129 23 352
Cuts at: 2897 3776 4373 5160 5428 1239
Size: 879 597 787 268 1524
Fragments arranged by size:
1524 879 787 597 451 393 352 268
192 129 102 23 16
EcoNI CCTnn'n_nnAGG
Cuts at: 2201 2201
Size: 5713
EcoO109I rG'GnC_Cy
Cuts at: 2797 2797
Size: 5713
EcoRI G'AATT_C
Cuts at: 3022 3400 3022
Size: 378 5335
FspI TGC'GCA
Cuts at: 4219 4219
Size: 5713
HaeII r_GCGC'y
Cuts at: 1678 2833 4860 1678
Size: 1155 2027 2531
Fragments arranged by size:
2531 2027 1155
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2870 2902 4354 4386 4428
Size: 32 135 32 1298 32 1452 32 42
Cuts at: 4428 4460 1373
Size: 32 2626
Fragments arranged by size:
2626 1452 1298 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3057 3303 669
Size: 988 281 1119 246 3079
Fragments arranged by size:
3079 1119 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5713
HpaI GTT'AAC
Cuts at: 3303 3303
Size: 5713
KasI G'GCGC_C
Cuts at: 2829 2829
Size: 5713
KpnI G_GTAC'C
Cuts at: 3038 3038
Size: 5713
MfeI C'AATT_G
Cuts at: 3312 3312
Size: 5713
MluI A'CGCG_T
Cuts at: 5589 5589
Size: 5713
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4827 5011 1305
Size: 3522 184 2007
Fragments arranged by size:
3522 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2360 10
Size: 54 1425 343 528 3363
Fragments arranged by size:
3363 1425 528 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2294 3671 4030 4189 518
Size: 648 1128 1377 359 159 2042
Fragments arranged by size:
2042 1377 1128 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2077 2331 2356 2587 2689 3129 3806 4954
Size: 471 254 25 231 102 440 677 1148
Cuts at: 4954 5199 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 471 440 254 245 231
102 25
NarI GG'CG_CC
Cuts at: 2830 2830
Size: 5713
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4222
Fragments arranged by size:
4222 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5713
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5713
NotI GC'GGCC_GC
Cuts at: 3160 3160
Size: 5713
NspI r_CATG'y
Cuts at: 1132 1229 3071 4616 1132
Size: 97 1842 1545 2229
Fragments arranged by size:
2229 1842 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2641 1348
Size: 1293 4420
PciI A'CATG_T
Cuts at: 4612 4612
Size: 5713
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2313 2568 2920 1108
Size: 1205 255 352 3901
Fragments arranged by size:
3901 1205 352 255
PmeI GTTT'AAAC
Cuts at: 3017 3017
Size: 5713
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3782 3814 5295 5327 3782
Size: 32 1481 32 4168
Fragments arranged by size:
4168 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5713
PsiI TTA'TAA
Cuts at: 3283 3283
Size: 5713
PstI C_TGCA'G
Cuts at: 830 2652 3065 830
Size: 1822 413 3478
Fragments arranged by size:
3478 1822 413
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5713
PvuII CAG'CTG
Cuts at: 2077 2356 2077
Size: 279 5434
SacI G_AGCT'C
Cuts at: 2578 2578
Size: 5713
SalI G'TCGA_C
Cuts at: 1936 3055 1936
Size: 1119 4594
SbfI CC_TGCA'GG
Cuts at: 3065 3065
Size: 5713
ScaI AGT'ACT
Cuts at: 3961 5673 3961
Size: 1712 4001
SfcI C'TryA_G
Cuts at: 826 1045 2648 3001 3061 4196 4877 5068 826
Size: 219 1603 353 60 1135 681 191 1471
Fragments arranged by size:
1603 1471 1135 681 353 219 191 60
SfiI GGCCn_nnn'nGGCC
Cuts at: 2069 2069
Size: 5713
SfoI GGC'GCC
Cuts at: 2831 2831
Size: 5713
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5713
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5713
SmaI CCC'GGG
Cuts at: 1872 3040 1872
Size: 1168 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3817 4718 4980 820
Size: 197 926 1874 901 262 1553
Fragments arranged by size:
1874 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3504 493
Size: 3011 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5713
SphI G_CATG'C
Cuts at: 1132 3071 1132
Size: 1939 3774
SspI AAT'ATT
Cuts at: 5 52 3637 5
Size: 47 3585 2081
Fragments arranged by size:
3585 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5713
StyI C'CwwG_G
Cuts at: 513 1065 2004 2307 2361 3152 513
Size: 552 939 303 54 791 3074
Fragments arranged by size:
3074 939 791 552 303 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3740 3757 3910 4095 4540 325
Size: 1255 2160 17 153 185 445 1498
Fragments arranged by size:
2160 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2154 2368 2673
Size: 274 80 33 51 1443 177 214 305
Cuts at: 2673 3959 5671 96
Size: 1286 1712 138
Fragments arranged by size:
1712 1443 1286 305 274 214 177 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5673 5705 5673
Size: 32 5681
Tth111I GACn'n_nGTC
Cuts at: 1316 2930 1316
Size: 1614 4099
XbaI T'CTAG_A
Cuts at: 3049 3049
Size: 5713
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2074 2074
Size: 5713
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5713
XmaI C'CCGG_G
Cuts at: 1870 3038 1870
Size: 1168 4545
XmnI GAAnn'nnTTC
Cuts at: 3842 3842
Size: 5713
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3521 276
Size: 53 83 186 936 1987 2468
Fragments arranged by size:
2468 1987 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SrfI SwaI