Restriction map for
pFN21AE6616
(Circular) (Six-base) MAPSORT of: pFN21AE6616.seq Check: 6847 from: 1 to: 5710
pFN21AE6616 5710 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3520 278
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2574 3053 1937
Size: 637 479 4594
Fragments arranged by size:
4594 637 479
Acc65I G'GTAC_C
Cuts at: 3031 3031
Size: 5710
AclI AA'CG_TT
Cuts at: 1563 3837 4210 1563
Size: 2274 373 3063
Fragments arranged by size:
3063 2274 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2033 2039 3773 5157 5425 1632
Size: 401 6 1734 1384 268 1917
Fragments arranged by size:
1917 1734 1384 401 268 6
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5513
AflIII A'CryG_T
Cuts at: 1203 2025 4609 5586 1203
Size: 822 2584 977 1327
Fragments arranged by size:
2584 1327 977 822
AgeI A'CCGG_T
Cuts at: 4541 4541
Size: 5710
AhdI GACnn_n'nnGTC
Cuts at: 4439 4439
Size: 5710
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5710
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5710
AlwNI CAG_nnn'CTG
Cuts at: 2302 3453 5025 2302
Size: 1151 1572 2987
Fragments arranged by size:
2987 1572 1151
ApaI G_GGCC'C
Cuts at: 2690 2690
Size: 5710
ApaLI G'TGCA_C
Cuts at: 3767 4923 3767
Size: 1156 4554
ApoI r'AATT_y
Cuts at: 1493 3019 3245 3397 5506 1493
Size: 1526 226 152 2109 1697
Fragments arranged by size:
2109 1697 1526 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4264 160
Size: 872 3232 1606
Fragments arranged by size:
3232 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
AvaI C'yCGr_G
Cuts at: 1870 1943 2216 2832 2910 3035 1870
Size: 73 273 616 78 125 4545
Fragments arranged by size:
4545 616 273 125 78 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2276 2309 2906 2939 2276
Size: 33 597 33 5047
Fragments arranged by size:
5047 597 33 33
BamHI G'GATC_C
Cuts at: 2872 3040 3407 2872
Size: 168 367 5175
Fragments arranged by size:
5175 367 168
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2311 3031 4486 618
Size: 325 217 627 524 720 1455 1842
Fragments arranged by size:
1842 1455 720 627 524 325 217
BanII G_rGCy'C
Cuts at: 1391 2690 2739 2990 3029 1391
Size: 1299 49 251 39 4072
Fragments arranged by size:
4072 1299 251 49 39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2846 928
Size: 971 947 3792
Fragments arranged by size:
3792 971 947
BbvCI CC'TCA_GC
Cuts at: 2346 2346
Size: 5710
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3901 3935 1903
Size: 34 1964 34 3678
Fragments arranged by size:
3678 1964 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3602 4812 3602
Size: 1210 4500
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5710
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2659 2743 4321
Size: 107 122 71 631 605 987 84 1578
Cuts at: 4321 136
Size: 1525
Fragments arranged by size:
1578 1525 987 631 605 122 107 84
71
BglII A'GATC_T
Cuts at: 5705 5705
Size: 5710
BlpI GC'TnA_GC
Cuts at: 3127 3127
Size: 5710
Bme1580I G_kGCm'C
Cuts at: 1790 2316 2690 3771 4927 1790
Size: 526 374 1081 1156 2573
Fragments arranged by size:
2573 1156 1081 526 374
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5710
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3441 4399 442
Size: 416 53 83 399 24 2024 958 1753
Fragments arranged by size:
2024 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2864 2906 4370 1239
Size: 1625 42 1464 2579
Fragments arranged by size:
2579 1625 1464 42
Bpu10I CC'TnA_GC
Cuts at: 2346 2346
Size: 5710
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3835 4700 4998 3835
Size: 865 298 4547
Fragments arranged by size:
4547 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2371 4373 882
Size: 617 872 2002 2219
Fragments arranged by size:
2219 2002 872 617
BsaAI yAC'GTr
Cuts at: 493 1204 3501 493
Size: 711 2297 2702
Fragments arranged by size:
2702 2297 711
BsaBI GATnn'nnATC
Cuts at: 5704 5704
Size: 5710
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3517 3899 4534 275
Size: 53 83 186 936 1984 382 635 1451
Fragments arranged by size:
1984 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4143 4541 4815 4962 5373 4143
Size: 398 274 147 411 4480
Fragments arranged by size:
4480 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5485
Fragments arranged by size:
5485 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 2384 2633 2975 1186
Size: 868 330 249 342 3921
Fragments arranged by size:
3921 868 342 330 249
BseYI C'CCAG_C
Cuts at: 2160 2202 2430 2457 2738 2995 4913 5409 2160
Size: 42 228 27 281 257 1918 496 2461
Fragments arranged by size:
2461 1918 496 281 257 228 42 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2890 1163
Size: 573 1154 3983
Fragments arranged by size:
3983 1154 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2055 2707 3160 3921 4949 1540
Size: 275 186 54 652 453 761 1028 2301
Fragments arranged by size:
2301 1028 761 652 453 275 186 54
BsiHKAI G_wGCw'C
Cuts at: 3771 3856 4927 3771
Size: 85 1071 4554
Fragments arranged by size:
4554 1071 85
BsmI GAATG_Cn'
Cuts at: 3221 3314 3221
Size: 93 5617
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2316 2690 2739 2990 3029 3771 3856
Size: 399 526 374 49 251 39 742 85
Cuts at: 3856 4927 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 526 399 374 251 85
49 39
BspEI T'CCGG_A
Cuts at: 5373 5373
Size: 5710
BspHI T'CATG_A
Cuts at: 3599 5329 3599
Size: 1730 3980
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3065 844
Size: 2221 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2828 3597 1394
Size: 429 1005 769 3507
Fragments arranged by size:
3507 1005 769 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4205 4379 65
Size: 1183 2957 174 1396
Fragments arranged by size:
2957 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2057 4354 4541 1700
Size: 110 247 2297 187 2869
Fragments arranged by size:
2869 2297 247 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5710
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5710
BssSI C'ACGA_G
Cuts at: 3770 4782 3770
Size: 1012 4698
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2329 794
Size: 1535 4175
BstBI TT'CG_AA
Cuts at: 4514 4514
Size: 5710
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5710
BstXI CCAn_nnnn'nTGG
Cuts at: 2362 2884 2362
Size: 522 5188
BstYI r'GATC_y
Cuts at: 1972 2146 2872 3040 3407 3792 3809 5261 5347
Size: 174 726 168 367 385 17 1452 86
Cuts at: 5347 5359 5705 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 726 385 367 346 174 168
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2691 2691
Size: 5710
BtgI C'CryG_G
Cuts at: 513 1065 2470 2635 2775 2891 513
Size: 552 1405 165 140 116 3332
Fragments arranged by size:
3332 1405 552 165 140 116
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4971
Fragments arranged by size:
4971 683 56
BtsI GCAGTG_nn'
Cuts at: 3226 4020 4040 3226
Size: 794 20 4896
Fragments arranged by size:
4896 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5710
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2988 3023 529
Size: 35 2424 35 3216
Fragments arranged by size:
3216 2424 35 35
DraI TTT'AAA
Cuts at: 3014 3014
Size: 5710
DraIII CAC_nnn'GTG
Cuts at: 2410 2869 2410
Size: 459 5251
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4717 809
Size: 507 3401 1802
Fragments arranged by size:
3401 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2086 2183 2704
Size: 54 1425 50 275 18 256 97 521
Cuts at: 2704 3157 4046 5675 8
Size: 453 889 1629 43
Fragments arranged by size:
1629 1425 889 521 453 275 256 97
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2704 3157 1537
Size: 275 892 453 4090
Fragments arranged by size:
4090 892 453 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2402 3640 1353
Size: 1049 1238 3423
Fragments arranged by size:
3423 1238 1049
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4297 4671 4817 387
Size: 1064 138 2708 374 146 1280
Fragments arranged by size:
2708 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2039 2864 2906 3773 4370 5157
Size: 393 401 6 825 42 867 597 787
Cuts at: 5157 5425 1239
Size: 268 1524
Fragments arranged by size:
1524 867 825 787 597 401 393 268
42 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2257 2257
Size: 5710
EcoO109I rG'GnC_Cy
Cuts at: 2221 2351 2494 2542 2559 2686 2687 2745 2881
Size: 130 143 48 17 127 1 58 136
Cuts at: 2881 2221
Size: 5050
Fragments arranged by size:
5050 143 136 130 127 58 48 17
1
EcoRI G'AATT_C
Cuts at: 3019 3397 3019
Size: 378 5332
FspI TGC'GCA
Cuts at: 4216 4216
Size: 5710
HaeII r_GCGC'y
Cuts at: 1678 4857 1678
Size: 3179 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4351 4383 4425 4457 1373
Size: 32 135 32 2779 32 42 32 2626
Fragments arranged by size:
2779 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3054 3300 669
Size: 988 281 1116 246 3079
Fragments arranged by size:
3079 1116 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5710
HpaI GTT'AAC
Cuts at: 3300 3300
Size: 5710
KpnI G_GTAC'C
Cuts at: 3035 3035
Size: 5710
MfeI C'AATT_G
Cuts at: 3000 3309 3000
Size: 309 5401
MluI A'CGCG_T
Cuts at: 5586 5586
Size: 5710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2203 4824 5008 1305
Size: 898 2621 184 2007
Fragments arranged by size:
2621 2007 898 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3888
Fragments arranged by size:
3888 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2469 3668 4027 4186 518
Size: 648 1303 1199 359 159 2042
Fragments arranged by size:
2042 1303 1199 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2164 2362 2434 2485 2968 2974 3126 3803
Size: 558 198 72 51 483 6 152 677
Cuts at: 3803 4951 5196 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 558 483 245 198 152
72 51 6
NaeI GCC'GGC
Cuts at: 2059 2059
Size: 5710
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5158
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5710
NgoMIV G'CCGG_C
Cuts at: 2057 2057
Size: 5710
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5710
NotI GC'GGCC_GC
Cuts at: 3157 3157
Size: 5710
NspI r_CATG'y
Cuts at: 1132 1229 3068 4613 1132
Size: 97 1839 1545 2229
Fragments arranged by size:
2229 1839 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5710
PciI A'CATG_T
Cuts at: 4609 4609
Size: 5710
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2869 1108
Size: 1761 3949
PmeI GTTT'AAAC
Cuts at: 3014 3014
Size: 5710
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3779 3811 5292 5324 3779
Size: 32 1481 32 4165
Fragments arranged by size:
4165 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2351 2542 2559 2881 2351
Size: 191 17 322 5180
Fragments arranged by size:
5180 322 191 17
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5710
PsiI TTA'TAA
Cuts at: 3280 3280
Size: 5710
PspOMI G'GGCC_C
Cuts at: 2686 2686
Size: 5710
PstI C_TGCA'G
Cuts at: 830 2973 3062 830
Size: 2143 89 3478
Fragments arranged by size:
3478 2143 89
PvuI CG_AT'CG
Cuts at: 2001 2055 2001
Size: 54 5656
PvuII CAG'CTG
Cuts at: 2164 2968 2164
Size: 804 4906
SalI G'TCGA_C
Cuts at: 1936 3052 1936
Size: 1116 4594
SanDI GG'GwC_CC
Cuts at: 2351 2559 2351
Size: 208 5502
SbfI CC_TGCA'GG
Cuts at: 3062 3062
Size: 5710
ScaI AGT'ACT
Cuts at: 3958 5670 3958
Size: 1712 3998
SexAI A'CCwGG_T
Cuts at: 2406 2406
Size: 5710
SfcI C'TryA_G
Cuts at: 826 1045 2599 2969 3058 4193 4874 5065 826
Size: 219 1554 370 89 1135 681 191 1471
Fragments arranged by size:
1554 1471 1135 681 370 219 191 89
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5710
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5710
SmaI CCC'GGG
Cuts at: 1872 2834 3037 1872
Size: 962 203 4545
Fragments arranged by size:
4545 962 203
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3814 4715 4977 820
Size: 197 926 1871 901 262 1553
Fragments arranged by size:
1871 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3501 493
Size: 3008 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5710
SphI G_CATG'C
Cuts at: 1132 3068 1132
Size: 1936 3774
SspI AAT'ATT
Cuts at: 5 52 3634 5
Size: 47 3582 2081
Fragments arranged by size:
3582 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5710
StyI C'CwwG_G
Cuts at: 513 1065 2563 2877 3149 513
Size: 552 1498 314 272 3074
Fragments arranged by size:
3074 1498 552 314 272
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2519 3737 3754 3907 4092 4537 325
Size: 1255 939 1218 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1218 939 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3956 5668 96
Size: 274 80 33 51 1443 1979 1712 138
Fragments arranged by size:
1979 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2623 2655 2760 2792 5670 5702 2623
Size: 32 105 32 2878 32 2631
Fragments arranged by size:
2878 2631 105 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5710
XbaI T'CTAG_A
Cuts at: 3046 3046
Size: 5710
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5710
XmaI C'CCGG_G
Cuts at: 1870 2832 3035 1870
Size: 962 203 4545
Fragments arranged by size:
4545 962 203
XmnI GAAnn'nnTTC
Cuts at: 3839 3839
Size: 5710
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3518 276
Size: 53 83 186 936 1984 2468
Fragments arranged by size:
2468 1984 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BfrBI BplI
BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SacI SacII SapI SfiI SfoI SrfI
SwaI XcmI