Restriction map for
pFN21AE6615
(Circular) (Six-base) MAPSORT of: pFN21AE6615.seq Check: 2298 from: 1 to: 5572
pFN21AE6615 5572 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3382 278
Size: 53 83 186 936 1846 2468
Fragments arranged by size:
2468 1846 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2915 1937
Size: 978 4594
Acc65I G'GTAC_C
Cuts at: 2802 2893 2802
Size: 91 5481
AclI AA'CG_TT
Cuts at: 1563 3699 4072 1563
Size: 2136 373 3063
Fragments arranged by size:
3063 2136 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3635 5019 5287 1632
Size: 2003 1384 268 1917
Fragments arranged by size:
2003 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5375
AflIII A'CryG_T
Cuts at: 1203 4471 5448 1203
Size: 3268 977 1327
Fragments arranged by size:
3268 1327 977
AgeI A'CCGG_T
Cuts at: 4403 4403
Size: 5572
AhdI GACnn_n'nnGTC
Cuts at: 2309 4301 2309
Size: 1992 3580
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5572
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2180 1184
Size: 996 4576
AlwNI CAG_nnn'CTG
Cuts at: 3315 4887 3315
Size: 1572 4000
ApaLI G'TGCA_C
Cuts at: 3629 4785 3629
Size: 1156 4416
ApoI r'AATT_y
Cuts at: 1493 2279 2881 3107 3259 5368 1493
Size: 786 602 226 152 2109 1697
Fragments arranged by size:
2109 1697 786 602 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4126 160
Size: 872 3094 1606
Fragments arranged by size:
3094 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5572
AvaI C'yCGr_G
Cuts at: 1870 1943 2383 2897 1870
Size: 73 440 514 4545
Fragments arranged by size:
4545 514 440 73
BamHI G'GATC_C
Cuts at: 2902 3269 2902
Size: 367 5205
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2802 2824 2893 4348 618
Size: 325 217 627 1015 22 69 1455 1842
Fragments arranged by size:
1842 1455 1015 627 325 217 69 22
BanII G_rGCy'C
Cuts at: 1391 2395 2406 2891 1391
Size: 1004 11 485 4072
Fragments arranged by size:
4072 1004 485 11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2148 928
Size: 971 249 4352
Fragments arranged by size:
4352 971 249
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2432 2466 3763 3797 1903
Size: 34 495 34 1297 34 3678
Fragments arranged by size:
3678 1297 495 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2554 3464 4674 2554
Size: 910 1210 3452
Fragments arranged by size:
3452 1210 910
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5572
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4183 136
Size: 107 122 71 631 605 2511 1525
Fragments arranged by size:
2511 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5567 5567
Size: 5572
BlpI GC'TnA_GC
Cuts at: 2989 2989
Size: 5572
Bme1580I G_kGCm'C
Cuts at: 1790 3633 4789 1790
Size: 1843 1156 2573
Fragments arranged by size:
2573 1843 1156
BmgBI CAC'GTC
Cuts at: 1318 2437 1318
Size: 1119 4453
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3303 4261 442
Size: 416 53 83 399 24 1886 958 1753
Fragments arranged by size:
1886 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2681 1056
Size: 1625 3947
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2409 2409
Size: 5572
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4232 1239
Size: 2993 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2507 2819 3697 4562 4860 2507
Size: 312 878 865 298 3219
Fragments arranged by size:
3219 878 865 312 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2277 4235 882
Size: 617 778 1958 2219
Fragments arranged by size:
2219 1958 778 617
BsaAI yAC'GTr
Cuts at: 493 1204 3363 493
Size: 711 2159 2702
Fragments arranged by size:
2702 2159 711
BsaBI GATnn'nnATC
Cuts at: 5566 5566
Size: 5572
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3379 3761 4396 275
Size: 53 83 186 936 1846 382 635 1451
Fragments arranged by size:
1846 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4005 4403 4677 4824 5235 4005
Size: 398 274 147 411 4342
Fragments arranged by size:
4342 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2704 2734 1178
Size: 30 165 30 1301 30 4016
Fragments arranged by size:
4016 1301 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2480 2489 2712 1186
Size: 1294 9 223 4046
Fragments arranged by size:
4046 1294 223 9
BseYI C'CCAG_C
Cuts at: 2508 2807 4775 5271 2508
Size: 299 1968 496 2809
Fragments arranged by size:
2809 1968 496 299
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2150 1163
Size: 573 414 4585
Fragments arranged by size:
4585 573 414
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3022 3783 4811 1540
Size: 275 186 1021 761 1028 2301
Fragments arranged by size:
2301 1028 1021 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2754 3633 3718 4789 2754
Size: 879 85 1071 3537
Fragments arranged by size:
3537 1071 879 85
BsmI GAATG_Cn'
Cuts at: 2698 3083 3176 2698
Size: 385 93 5094
Fragments arranged by size:
5094 385 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2296 2296
Size: 5572
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2395 2406 2754 2891 3633 3718 4789
Size: 399 605 11 348 137 742 85 1071
Cuts at: 4789 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 605 399 348 137 85
11
BspEI T'CCGG_A
Cuts at: 5235 5235
Size: 5572
BspHI T'CATG_A
Cuts at: 3461 5191 3461
Size: 1730 3842
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2172 2687 2927 844
Size: 1328 515 240 3489
Fragments arranged by size:
3489 1328 515 240
BsrBI CCG'CTC
Cuts at: 1394 1823 3459 1394
Size: 429 1636 3507
Fragments arranged by size:
3507 1636 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4067 4241 65
Size: 1183 2819 174 1396
Fragments arranged by size:
2819 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4216 4403 1700
Size: 110 2406 187 2869
Fragments arranged by size:
2869 2406 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5572
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5572
BssSI C'ACGA_G
Cuts at: 3632 4644 3632
Size: 1012 4560
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5572
BstBI TT'CG_AA
Cuts at: 2452 4376 2452
Size: 1924 3648
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5572
BstYI r'GATC_y
Cuts at: 1972 2058 2902 3269 3654 3671 5123 5209 5221
Size: 86 844 367 385 17 1452 86 12
Cuts at: 5221 5567 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 844 385 367 346 86 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2796 2820 513
Size: 552 1731 24 3265
Fragments arranged by size:
3265 1731 552 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2489 2644 487
Size: 683 56 1263 155 3415
Fragments arranged by size:
3415 1263 683 155 56
BtsI GCAGTG_nn'
Cuts at: 2164 3088 3882 3902 2164
Size: 924 794 20 3834
Fragments arranged by size:
3834 924 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5572
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5537
DraI TTT'AAA
Cuts at: 2065 2876 2065
Size: 811 4761
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4579 809
Size: 507 3263 1802
Fragments arranged by size:
3263 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3019 3908 5537
Size: 54 1425 50 275 18 1189 889 1629
Cuts at: 5537 8
Size: 43
Fragments arranged by size:
1629 1425 1189 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3019 1537
Size: 275 1207 4090
Fragments arranged by size:
4090 1207 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2466 3502 1353
Size: 1113 1036 3423
Fragments arranged by size:
3423 1113 1036
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4159 4533 4679 387
Size: 1064 138 2570 374 146 1280
Fragments arranged by size:
2570 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3635 4232 5019 5287 1239
Size: 393 2003 597 787 268 1524
Fragments arranged by size:
2003 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2428 2428
Size: 5572
EcoRI G'AATT_C
Cuts at: 2881 3259 2881
Size: 378 5194
FspI TGC'GCA
Cuts at: 4078 4078
Size: 5572
HaeII r_GCGC'y
Cuts at: 1678 4719 1678
Size: 3041 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2295 2327 2488 2520 4213
Size: 32 135 32 723 32 161 32 1693
Cuts at: 4213 4245 4287 4319 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1693 723 161 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2916 3162 669
Size: 988 281 978 246 3079
Fragments arranged by size:
3079 988 978 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5572
HpaI GTT'AAC
Cuts at: 3162 3162
Size: 5572
KpnI G_GTAC'C
Cuts at: 2806 2897 2806
Size: 91 5481
MfeI C'AATT_G
Cuts at: 3171 3171
Size: 5572
MluI A'CGCG_T
Cuts at: 5448 5448
Size: 5572
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4686 4870 1305
Size: 3381 184 2007
Fragments arranged by size:
3381 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3750
Fragments arranged by size:
3750 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3530 3889 4048 518
Size: 648 2364 359 159 2042
Fragments arranged by size:
2364 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2988 3665 4813 5058 1606
Size: 1382 677 1148 245 2120
Fragments arranged by size:
2120 1382 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5020
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5572
NheI G'CTAG_C
Cuts at: 1052 2677 1052
Size: 1625 3947
NotI GC'GGCC_GC
Cuts at: 3019 3019
Size: 5572
NspI r_CATG'y
Cuts at: 1132 1229 2930 4475 1132
Size: 97 1701 1545 2229
Fragments arranged by size:
2229 1701 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2130 1348
Size: 782 4790
PciI A'CATG_T
Cuts at: 4471 4471
Size: 5572
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2860 1108
Size: 1752 3820
PmeI GTTT'AAAC
Cuts at: 2876 2876
Size: 5572
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3641 3673 5154 5186 3641
Size: 32 1481 32 4027
Fragments arranged by size:
4027 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2428 2428
Size: 5572
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5572
PsiI TTA'TAA
Cuts at: 3142 3142
Size: 5572
PstI C_TGCA'G
Cuts at: 830 2691 2924 830
Size: 1861 233 3478
Fragments arranged by size:
3478 1861 233
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5572
SalI G'TCGA_C
Cuts at: 1936 2914 1936
Size: 978 4594
SbfI CC_TGCA'GG
Cuts at: 2924 2924
Size: 5572
ScaI AGT'ACT
Cuts at: 2555 3820 5532 2555
Size: 1265 1712 2595
Fragments arranged by size:
2595 1712 1265
SfcI C'TryA_G
Cuts at: 826 1045 2687 2920 4055 4736 4927 826
Size: 219 1642 233 1135 681 191 1471
Fragments arranged by size:
1642 1471 1135 681 233 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5572
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5572
SmaI CCC'GGG
Cuts at: 1872 2899 1872
Size: 1027 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2522 2834 3676 4577 4839 820
Size: 197 926 579 312 842 901 262 1553
Fragments arranged by size:
1553 926 901 842 579 312 262 197
SnaBI TAC'GTA
Cuts at: 493 3363 493
Size: 2870 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5572
SphI G_CATG'C
Cuts at: 1132 2930 1132
Size: 1798 3774
SspI AAT'ATT
Cuts at: 5 52 3496 5
Size: 47 3444 2081
Fragments arranged by size:
3444 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5572
StyI C'CwwG_G
Cuts at: 513 1065 3011 513
Size: 552 1946 3074
Fragments arranged by size:
3074 1946 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2122 3599 3616 3769 3954 4399 325
Size: 1255 542 1477 17 153 185 445 1498
Fragments arranged by size:
1498 1477 1255 542 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2553 2596 3818
Size: 274 80 33 51 1443 576 43 1222
Cuts at: 3818 5530 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1222 576 274 138 80 51
43 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2704 2736 5532 5564 2704
Size: 32 2796 32 2712
Fragments arranged by size:
2796 2712 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5572
XbaI T'CTAG_A
Cuts at: 2908 2908
Size: 5572
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5572
XmaI C'CCGG_G
Cuts at: 1870 2897 1870
Size: 1027 4545
XmnI GAAnn'nnTTC
Cuts at: 3701 3701
Size: 5572
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3380 276
Size: 53 83 186 936 1846 2468
Fragments arranged by size:
2468 1846 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI