Restriction map for
pFN21AE6612
(Circular) (Six-base) MAPSORT of: pFN21AE6612.seq Check: 5644 from: 1 to: 5638
pFN21AE6612 5638 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3448 278
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2981 1937
Size: 1044 4594
Acc65I G'GTAC_C
Cuts at: 2959 2959
Size: 5638
AclI AA'CG_TT
Cuts at: 1563 3765 4138 1563
Size: 2202 373 3063
Fragments arranged by size:
3063 2202 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2301 2405 2641 3701 5085 5353 1632
Size: 669 104 236 1060 1384 268 1917
Fragments arranged by size:
1917 1384 1060 669 268 236 104
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5441
AflIII A'CryG_T
Cuts at: 1203 2539 4537 5514 1203
Size: 1336 1998 977 1327
Fragments arranged by size:
1998 1336 1327 977
AgeI A'CCGG_T
Cuts at: 4469 4469
Size: 5638
AhdI GACnn_n'nnGTC
Cuts at: 4367 4367
Size: 5638
AleI CACnn'nnGTG
Cuts at: 1166 2897 1166
Size: 1731 3907
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5638
AlwNI CAG_nnn'CTG
Cuts at: 2265 2600 2928 3381 4953 2265
Size: 335 328 453 1572 2950
Fragments arranged by size:
2950 1572 453 335 328
ApaI G_GGCC'C
Cuts at: 2487 2487
Size: 5638
ApaLI G'TGCA_C
Cuts at: 2573 3695 4851 2573
Size: 1122 1156 3360
Fragments arranged by size:
3360 1156 1122
ApoI r'AATT_y
Cuts at: 1493 2947 3173 3325 5434 1493
Size: 1454 226 152 2109 1697
Fragments arranged by size:
2109 1697 1454 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4192 160
Size: 872 3160 1606
Fragments arranged by size:
3160 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
AvaI C'yCGr_G
Cuts at: 1870 1943 2963 1870
Size: 73 1020 4545
Fragments arranged by size:
4545 1020 73
BamHI G'GATC_C
Cuts at: 2253 2968 3335 2253
Size: 715 367 4556
Fragments arranged by size:
4556 715 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2528 2959 4414 618
Size: 325 217 627 741 431 1455 1842
Fragments arranged by size:
1842 1455 741 627 431 325 217
BanII G_rGCy'C
Cuts at: 1391 2171 2360 2487 2957 1391
Size: 780 189 127 470 4072
Fragments arranged by size:
4072 780 470 189 127
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4667
BbvCI CC'TCA_GC
Cuts at: 2368 2368
Size: 5638
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3829 3863 1903
Size: 34 50 34 1808 34 3678
Fragments arranged by size:
3678 1808 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3530 4740 3530
Size: 1210 4428
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5638
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4249 136
Size: 107 122 71 631 605 2577 1525
Fragments arranged by size:
2577 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5633 5633
Size: 5638
BlpI GC'TnA_GC
Cuts at: 3055 3055
Size: 5638
Bme1580I G_kGCm'C
Cuts at: 1790 2487 2577 3699 4855 1790
Size: 697 90 1122 1156 2573
Fragments arranged by size:
2573 1156 1122 697 90
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5638
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2165 2482 3369
Size: 416 53 83 399 24 748 317 887
Cuts at: 3369 4327 442
Size: 958 1753
Fragments arranged by size:
1753 958 887 748 416 399 317 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2205 2395 2492 2533 2771 4298 1239
Size: 966 190 97 41 238 1527 2579
Fragments arranged by size:
2579 1527 966 238 190 97 41
Bpu10I CC'TnA_GC
Cuts at: 2368 2368
Size: 5638
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3763 4628 4926 3763
Size: 865 298 4475
Fragments arranged by size:
4475 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4301 882
Size: 617 2802 2219
Fragments arranged by size:
2802 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3429 493
Size: 711 2225 2702
Fragments arranged by size:
2702 2225 711
BsaBI GATnn'nnATC
Cuts at: 2435 5632 2435
Size: 3197 2441
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3445 3827 4462 275
Size: 53 83 186 936 1912 382 635 1451
Fragments arranged by size:
1912 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4071 4469 4743 4890 5301 4071
Size: 398 274 147 411 4408
Fragments arranged by size:
4408 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2178 2208 2548 2578 1178
Size: 30 165 30 775 30 340 30 4238
Fragments arranged by size:
4238 775 340 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2530 2569 2888 2909 1186
Size: 1344 39 319 21 3915
Fragments arranged by size:
3915 1344 319 39 21
BseYI C'CCAG_C
Cuts at: 2258 4841 5337 2258
Size: 2583 496 2559
Fragments arranged by size:
2583 2559 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5065
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3088 3849 4877 1540
Size: 275 186 1087 761 1028 2301
Fragments arranged by size:
2301 1087 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2273 2577 3699 3784 4855 2273
Size: 304 1122 85 1071 3056
Fragments arranged by size:
3056 1122 1071 304 85
BsmI GAATG_Cn'
Cuts at: 3149 3242 3149
Size: 93 5545
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2171 2273 2360 2487 2577 2957 3699
Size: 399 381 102 87 127 90 380 742
Cuts at: 3699 3784 4855 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 381 380 127 102
90 87 85
BspEI T'CCGG_A
Cuts at: 5301 5301
Size: 5638
BspHI T'CATG_A
Cuts at: 3527 5257 3527
Size: 1730 3908
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2993 844
Size: 2149 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3525 1394
Size: 429 1702 3507
Fragments arranged by size:
3507 1702 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4133 4307 65
Size: 1183 2885 174 1396
Fragments arranged by size:
2885 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2778 4282 4469 1700
Size: 110 968 1504 187 2869
Fragments arranged by size:
2869 1504 968 187 110
BsrGI T'GTAC_A
Cuts at: 96 2235 96
Size: 2139 3499
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5638
BssSI C'ACGA_G
Cuts at: 2239 3698 4710 2239
Size: 1459 1012 3167
Fragments arranged by size:
3167 1459 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2268 794
Size: 1474 4164
BstBI TT'CG_AA
Cuts at: 4442 4442
Size: 5638
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5638
BstXI CCAn_nnnn'nTGG
Cuts at: 2701 2701
Size: 5638
BstYI r'GATC_y
Cuts at: 1972 2253 2931 2968 3335 3720 3737 5189 5275
Size: 281 678 37 367 385 17 1452 86
Cuts at: 5275 5287 5633 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 678 385 367 346 281 86
37 17 12
Bsu36I CC'TnA_GG
Cuts at: 2910 2921 2910
Size: 11 5627
BtgI C'CryG_G
Cuts at: 513 1065 2586 2805 2892 513
Size: 552 1521 219 87 3259
Fragments arranged by size:
3259 1521 552 219 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4899
Fragments arranged by size:
4899 683 56
BtsI GCAGTG_nn'
Cuts at: 3154 3948 3968 3154
Size: 794 20 4824
Fragments arranged by size:
4824 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5638
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5603
DraI TTT'AAA
Cuts at: 2942 2942
Size: 5638
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4645 809
Size: 507 3329 1802
Fragments arranged by size:
3329 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2100 2316 3085
Size: 54 1425 50 275 18 270 216 769
Cuts at: 3085 3974 5603 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 769 275 270 216 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3085 1537
Size: 275 1273 4090
Fragments arranged by size:
4090 1273 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3568 1353
Size: 2215 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4225 4599 4745 387
Size: 1064 138 2636 374 146 1280
Fragments arranged by size:
2636 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2205 2301 2395 2405 2492 2533 2641
Size: 393 573 96 94 10 87 41 108
Cuts at: 2641 2771 3701 4298 5085 5353 1239
Size: 130 930 597 787 268 1524
Fragments arranged by size:
1524 930 787 597 573 393 268 130
108 96 94 87 41 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2512 2512
Size: 5638
EcoO109I rG'GnC_Cy
Cuts at: 2285 2476 2483 2484 2925 2285
Size: 191 7 1 441 4998
Fragments arranged by size:
4998 441 191 7 1
EcoRI G'AATT_C
Cuts at: 2947 3325 2947
Size: 378 5260
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2173 2410 2173
Size: 237 5401
FspI TGC'GCA
Cuts at: 4144 4144
Size: 5638
HaeII r_GCGC'y
Cuts at: 1678 2655 4785 1678
Size: 977 2130 2531
Fragments arranged by size:
2531 2130 977
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4279 4311 4353 4385 1373
Size: 32 135 32 2707 32 42 32 2626
Fragments arranged by size:
2707 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2982 3228 669
Size: 988 281 1044 246 3079
Fragments arranged by size:
3079 1044 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5638
HpaI GTT'AAC
Cuts at: 3228 3228
Size: 5638
KpnI G_GTAC'C
Cuts at: 2963 2963
Size: 5638
MfeI C'AATT_G
Cuts at: 3237 3237
Size: 5638
MluI A'CGCG_T
Cuts at: 5514 5514
Size: 5638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4752 4936 1305
Size: 3447 184 2007
Fragments arranged by size:
3447 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2102 10
Size: 54 1425 343 270 3546
Fragments arranged by size:
3546 1425 343 270 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2897 3596 3955 4114 518
Size: 648 1731 699 359 159 2042
Fragments arranged by size:
2042 1731 699 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2265 2862 3054 3731 4879 5124 1606
Size: 659 597 192 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 659 597 245 192
NaeI GCC'GGC
Cuts at: 2780 2780
Size: 5638
NcoI C'CATG_G
Cuts at: 513 1065 2586 513
Size: 552 1521 3565
Fragments arranged by size:
3565 1521 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5638
NgoMIV G'CCGG_C
Cuts at: 2778 2778
Size: 5638
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5638
NotI GC'GGCC_GC
Cuts at: 3085 3085
Size: 5638
NspI r_CATG'y
Cuts at: 1132 1229 2543 2996 4541 1132
Size: 97 1314 453 1545 2229
Fragments arranged by size:
2229 1545 1314 453 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5638
PciI A'CATG_T
Cuts at: 2539 4537 2539
Size: 1998 3640
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2265 1108
Size: 1157 4481
PmeI GTTT'AAAC
Cuts at: 2942 2942
Size: 5638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3707 3739 5220 5252 3707
Size: 32 1481 32 4093
Fragments arranged by size:
4093 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2285 2476 2285
Size: 191 5447
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5638
PsiI TTA'TAA
Cuts at: 3208 3208
Size: 5638
PspOMI G'GGCC_C
Cuts at: 2483 2483
Size: 5638
PstI C_TGCA'G
Cuts at: 830 2867 2990 830
Size: 2037 123 3478
Fragments arranged by size:
3478 2037 123
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5638
PvuII CAG'CTG
Cuts at: 2265 2862 2265
Size: 597 5041
SalI G'TCGA_C
Cuts at: 1936 2980 1936
Size: 1044 4594
SbfI CC_TGCA'GG
Cuts at: 2990 2990
Size: 5638
ScaI AGT'ACT
Cuts at: 2792 3886 5598 2792
Size: 1094 1712 2832
Fragments arranged by size:
2832 1712 1094
SexAI A'CCwGG_T
Cuts at: 2457 2457
Size: 5638
SfcI C'TryA_G
Cuts at: 826 1045 2863 2986 4121 4802 4993 826
Size: 219 1818 123 1135 681 191 1471
Fragments arranged by size:
1818 1471 1135 681 219 191 123
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5638
SmaI CCC'GGG
Cuts at: 1872 2965 1872
Size: 1093 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3742 4643 4905 820
Size: 197 926 1799 901 262 1553
Fragments arranged by size:
1799 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3429 493
Size: 2936 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5638
SphI G_CATG'C
Cuts at: 1132 2996 1132
Size: 1864 3774
SspI AAT'ATT
Cuts at: 5 52 3562 5
Size: 47 3510 2081
Fragments arranged by size:
3510 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5638
StyI C'CwwG_G
Cuts at: 513 1065 2586 3077 513
Size: 552 1521 491 3074
Fragments arranged by size:
3074 1521 552 491
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3665 3682 3835 4020 4465 325
Size: 1255 2085 17 153 185 445 1498
Fragments arranged by size:
2085 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2131 2196 2235
Size: 274 80 33 51 1443 154 65 39
Cuts at: 2235 2790 3884 5596 96
Size: 555 1094 1712 138
Fragments arranged by size:
1712 1443 1094 555 274 154 138 80
65 51 39 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2178 2210 2269 2301 5598 5630 2178
Size: 32 59 32 3297 32 2186
Fragments arranged by size:
3297 2186 59 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5638
XbaI T'CTAG_A
Cuts at: 2026 2974 2026
Size: 948 4690
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2763 2763
Size: 5638
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5638
XmaI C'CCGG_G
Cuts at: 1870 2963 1870
Size: 1093 4545
XmnI GAAnn'nnTTC
Cuts at: 3767 3767
Size: 5638
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3446 276
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PspXI PsrI RsrII SacI SacII SanDI SapI SfiI
SfoI SrfI SwaI