Restriction map for
pFN21AE6611
(Circular) (Six-base) MAPSORT of: pFN21AE6611.seq Check: 8297 from: 1 to: 5506
pFN21AE6611 5506 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3316 278
Size: 53 83 186 936 1780 2468
Fragments arranged by size:
2468 1780 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2849 1937
Size: 912 4594
Acc65I G'GTAC_C
Cuts at: 2827 2827
Size: 5506
AclI AA'CG_TT
Cuts at: 1563 3633 4006 1563
Size: 2070 373 3063
Fragments arranged by size:
3063 2070 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2055 2745 3569 4953 5221 1632
Size: 423 690 824 1384 268 1917
Fragments arranged by size:
1917 1384 824 690 423 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5309
AflIII A'CryG_T
Cuts at: 1203 4405 5382 1203
Size: 3202 977 1327
Fragments arranged by size:
3202 1327 977
AgeI A'CCGG_T
Cuts at: 4337 4337
Size: 5506
AhdI GACnn_n'nnGTC
Cuts at: 4235 4235
Size: 5506
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5506
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5506
AlwNI CAG_nnn'CTG
Cuts at: 2104 3249 4821 2104
Size: 1145 1572 2789
Fragments arranged by size:
2789 1572 1145
ApaLI G'TGCA_C
Cuts at: 3563 4719 3563
Size: 1156 4350
ApoI r'AATT_y
Cuts at: 1493 2815 3041 3193 5302 1493
Size: 1322 226 152 2109 1697
Fragments arranged by size:
2109 1697 1322 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4060 160
Size: 872 3028 1606
Fragments arranged by size:
3028 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5506
AvaI C'yCGr_G
Cuts at: 1870 1943 2831 1870
Size: 73 888 4545
Fragments arranged by size:
4545 888 73
BamHI G'GATC_C
Cuts at: 2836 3203 2836
Size: 367 5139
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2827 4282 618
Size: 325 217 627 1040 1455 1842
Fragments arranged by size:
1842 1455 1040 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2825 1391
Size: 1434 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2748 928
Size: 971 849 3686
Fragments arranged by size:
3686 971 849
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3697 3731 1903
Size: 34 1760 34 3678
Fragments arranged by size:
3678 1760 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2387 3398 4608 2387
Size: 1011 1210 3285
Fragments arranged by size:
3285 1210 1011
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5506
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4117 136
Size: 107 122 71 631 605 2445 1525
Fragments arranged by size:
2445 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5501 5501
Size: 5506
BlpI GC'TnA_GC
Cuts at: 2142 2151 2923 2142
Size: 9 772 4725
Fragments arranged by size:
4725 772 9
Bme1580I G_kGCm'C
Cuts at: 1790 3567 4723 1790
Size: 1777 1156 2573
Fragments arranged by size:
2573 1777 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5506
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2593 3237 4195
Size: 416 53 83 399 24 1176 644 958
Cuts at: 4195 442
Size: 1753
Fragments arranged by size:
1753 1176 958 644 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2190 1056
Size: 1134 4372
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4166 1239
Size: 2927 2579
Bpu10I CC'TnA_GC
Cuts at: 2133 2133
Size: 5506
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3631 4496 4794 3631
Size: 865 298 4343
Fragments arranged by size:
4343 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4169 882
Size: 617 2670 2219
Fragments arranged by size:
2670 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3297 493
Size: 711 2093 2702
Fragments arranged by size:
2702 2093 711
BsaBI GATnn'nnATC
Cuts at: 2288 5500 2288
Size: 3212 2294
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3313 3695 4330 275
Size: 53 83 186 936 1780 382 635 1451
Fragments arranged by size:
1780 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3939 4337 4611 4758 5169 3939
Size: 398 274 147 411 4276
Fragments arranged by size:
4276 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5281
Fragments arranged by size:
5281 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2757 1186
Size: 1571 3935
BseYI C'CCAG_C
Cuts at: 4709 5205 4709
Size: 496 5010
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4933
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2956 3717 4745 1540
Size: 275 186 955 761 1028 2301
Fragments arranged by size:
2301 1028 955 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2143 2152 3567 3652 4723 2143
Size: 9 1415 85 1071 2926
Fragments arranged by size:
2926 1415 1071 85 9
BsmI GAATG_Cn'
Cuts at: 3017 3110 3017
Size: 93 5413
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2143 2152 2825 3567 3652 4723 1391
Size: 399 353 9 673 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 673 399 353 85 9
BspEI T'CCGG_A
Cuts at: 5169 5169
Size: 5506
BspHI T'CATG_A
Cuts at: 3395 5125 3395
Size: 1730 3776
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2861 844
Size: 2017 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3393 1394
Size: 429 1570 3507
Fragments arranged by size:
3507 1570 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4001 4175 65
Size: 1183 2753 174 1396
Fragments arranged by size:
2753 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4150 4337 1700
Size: 110 2340 187 2869
Fragments arranged by size:
2869 2340 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5506
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5506
BssSI C'ACGA_G
Cuts at: 3566 4578 3566
Size: 1012 4494
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5506
BstBI TT'CG_AA
Cuts at: 4310 4310
Size: 5506
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5506
BstYI r'GATC_y
Cuts at: 1972 2836 3203 3588 3605 5057 5143 5155 5501
Size: 864 367 385 17 1452 86 12 346
Cuts at: 5501 1972
Size: 1977
Fragments arranged by size:
1977 1452 864 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4954
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4767
Fragments arranged by size:
4767 683 56
BtsI GCAGTG_nn'
Cuts at: 3022 3816 3836 3022
Size: 794 20 4692
Fragments arranged by size:
4692 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5506
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5471
DraI TTT'AAA
Cuts at: 2257 2810 2257
Size: 553 4953
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2752 4513 809
Size: 507 1436 1761 1802
Fragments arranged by size:
1802 1761 1436 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2572 2953 3842
Size: 54 1425 50 275 18 742 381 889
Cuts at: 3842 5471 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 742 381 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2953 1537
Size: 275 1141 4090
Fragments arranged by size:
4090 1141 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3436 1353
Size: 2083 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4093 4467 4613 387
Size: 1064 138 2504 374 146 1280
Fragments arranged by size:
2504 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2055 2745 3569 4166 4953 5221 1239
Size: 393 423 690 824 597 787 268 1524
Fragments arranged by size:
1524 824 787 690 597 423 393 268
EcoRI G'AATT_C
Cuts at: 2815 3193 2815
Size: 378 5128
FspI TGC'GCA
Cuts at: 4012 4012
Size: 5506
HaeII r_GCGC'y
Cuts at: 1678 4653 1678
Size: 2975 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4147 4179 4221 4253 1373
Size: 32 135 32 2575 32 42 32 2626
Fragments arranged by size:
2626 2575 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2195 2850 3096 669
Size: 988 281 257 655 246 3079
Fragments arranged by size:
3079 988 655 281 257 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5506
HpaI GTT'AAC
Cuts at: 3096 3096
Size: 5506
KpnI G_GTAC'C
Cuts at: 2831 2831
Size: 5506
MfeI C'AATT_G
Cuts at: 3105 3105
Size: 5506
MluI A'CGCG_T
Cuts at: 5382 5382
Size: 5506
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2278 4620 4804 1305
Size: 973 2342 184 2007
Fragments arranged by size:
2342 2007 973 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2574 10
Size: 54 1425 343 742 2942
Fragments arranged by size:
2942 1425 742 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2528 2621 3464 3823 3982 518
Size: 648 1362 93 843 359 159 2042
Fragments arranged by size:
2042 1362 843 648 359 159 93
MspA1I CmG'CkG
Cuts at: 1606 2922 3599 4747 4992 1606
Size: 1316 677 1148 245 2120
Fragments arranged by size:
2120 1316 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4954
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5506
NheI G'CTAG_C
Cuts at: 1052 2186 1052
Size: 1134 4372
NotI GC'GGCC_GC
Cuts at: 2953 2953
Size: 5506
NspI r_CATG'y
Cuts at: 1132 1229 2864 4409 1132
Size: 97 1635 1545 2229
Fragments arranged by size:
2229 1635 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5506
PciI A'CATG_T
Cuts at: 4405 4405
Size: 5506
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2293 1108
Size: 1185 4321
PfoI T'CCnGG_A
Cuts at: 2086 2173 2086
Size: 87 5419
PmeI GTTT'AAAC
Cuts at: 2810 2810
Size: 5506
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3575 3607 5088 5120 3575
Size: 32 1481 32 3961
Fragments arranged by size:
3961 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5506
PsiI TTA'TAA
Cuts at: 3076 3076
Size: 5506
PstI C_TGCA'G
Cuts at: 830 2858 830
Size: 2028 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5506
SalI G'TCGA_C
Cuts at: 1936 2848 1936
Size: 912 4594
SbfI CC_TGCA'GG
Cuts at: 2858 2858
Size: 5506
ScaI AGT'ACT
Cuts at: 3754 5466 3754
Size: 1712 3794
SfcI C'TryA_G
Cuts at: 826 1045 2854 3989 4670 4861 826
Size: 219 1809 1135 681 191 1471
Fragments arranged by size:
1809 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5506
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5506
SmaI CCC'GGG
Cuts at: 1872 2833 1872
Size: 961 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3610 4511 4773 820
Size: 197 926 1667 901 262 1553
Fragments arranged by size:
1667 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3297 493
Size: 2804 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5506
SphI G_CATG'C
Cuts at: 1132 2864 1132
Size: 1732 3774
SspI AAT'ATT
Cuts at: 5 52 3430 5
Size: 47 3378 2081
Fragments arranged by size:
3378 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5506
StyI C'CwwG_G
Cuts at: 513 1065 2454 2945 513
Size: 552 1389 491 3074
Fragments arranged by size:
3074 1389 552 491
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3533 3550 3703 3888 4333 325
Size: 1255 1953 17 153 185 445 1498
Fragments arranged by size:
1953 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3752 5464 96
Size: 274 80 33 51 1443 1775 1712 138
Fragments arranged by size:
1775 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5466 5498 5466
Size: 32 5474
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5506
XbaI T'CTAG_A
Cuts at: 2842 2842
Size: 5506
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2294 2294
Size: 5506
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5506
XmaI C'CCGG_G
Cuts at: 1870 2831 1870
Size: 961 4545
XmnI GAAnn'nnTTC
Cuts at: 3635 3635
Size: 5506
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3314 276
Size: 53 83 186 936 1780 2468
Fragments arranged by size:
2468 1780 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI