Restriction map for
pFN21AE6606
(Circular) (Six-base) MAPSORT of: pFN21AE6606.seq Check: 3086 from: 1 to: 5707
pFN21AE6606 5707 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3517 278
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3050 1937
Size: 1113 4594
Acc65I G'GTAC_C
Cuts at: 2514 3028 2514
Size: 514 5193
AclI AA'CG_TT
Cuts at: 1563 3006 3834 4207 1563
Size: 1443 828 373 3063
Fragments arranged by size:
3063 1443 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3770 5154 5422 1632
Size: 2138 1384 268 1917
Fragments arranged by size:
2138 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5510
AflIII A'CryG_T
Cuts at: 1203 2652 2905 4606 5583 1203
Size: 1449 253 1701 977 1327
Fragments arranged by size:
1701 1449 1327 977 253
AgeI A'CCGG_T
Cuts at: 4538 4538
Size: 5707
AhdI GACnn_n'nnGTC
Cuts at: 4436 4436
Size: 5707
AleI CACnn'nnGTG
Cuts at: 1166 2297 2621 1166
Size: 1131 324 4252
Fragments arranged by size:
4252 1131 324
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5707
AlwNI CAG_nnn'CTG
Cuts at: 2018 3450 5022 2018
Size: 1432 1572 2703
Fragments arranged by size:
2703 1572 1432
ApaI G_GGCC'C
Cuts at: 2109 2109
Size: 5707
ApaLI G'TGCA_C
Cuts at: 2073 3764 4920 2073
Size: 1691 1156 2860
Fragments arranged by size:
2860 1691 1156
ApoI r'AATT_y
Cuts at: 1493 3016 3242 3394 5503 1493
Size: 1523 226 152 2109 1697
Fragments arranged by size:
2109 1697 1523 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4261 160
Size: 872 3229 1606
Fragments arranged by size:
3229 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
AvaI C'yCGr_G
Cuts at: 1870 1943 2168 3032 1870
Size: 73 225 864 4545
Fragments arranged by size:
4545 864 225 73
BamHI G'GATC_C
Cuts at: 3037 3404 3037
Size: 367 5340
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2366 2514 3028 4483 618
Size: 325 217 627 579 148 514 1455 1842
Fragments arranged by size:
1842 1455 627 579 514 325 217 148
BanII G_rGCy'C
Cuts at: 1391 2109 2991 3026 1391
Size: 718 882 35 4072
Fragments arranged by size:
4072 882 718 35
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2481 928
Size: 971 582 4154
Fragments arranged by size:
4154 971 582
BbvCI CC'TCA_GC
Cuts at: 2063 2181 2063
Size: 118 5589
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2159 2193 3898 3932 1903
Size: 34 222 34 1705 34 3678
Fragments arranged by size:
3678 1705 222 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3599 4809 3599
Size: 1210 4497
BclI T'GATC_A
Cuts at: 1548 2625 1548
Size: 1077 4630
BfrBI ATG'CAT
Cuts at: 2799 2799
Size: 5707
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4318 136
Size: 107 122 71 631 605 2646 1525
Fragments arranged by size:
2646 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5702 5702
Size: 5707
BlpI GC'TnA_GC
Cuts at: 2985 3124 2985
Size: 139 5568
Bme1580I G_kGCm'C
Cuts at: 1790 2077 2109 3768 4924 1790
Size: 287 32 1659 1156 2573
Fragments arranged by size:
2573 1659 1156 287 32
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5707
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2083 2724 3438
Size: 416 53 83 399 24 666 641 714
Cuts at: 3438 4396 442
Size: 958 1753
Fragments arranged by size:
1753 958 714 666 641 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2513 2915 4367 1239
Size: 1274 402 1452 2579
Fragments arranged by size:
2579 1452 1274 402
Bpu10I CC'TnA_GC
Cuts at: 2063 2181 2063
Size: 118 5589
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3832 4697 4995 3832
Size: 865 298 4544
Fragments arranged by size:
4544 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2086 2214 2584 4370 882
Size: 617 587 128 370 1786 2219
Fragments arranged by size:
2219 1786 617 587 370 128
BsaAI yAC'GTr
Cuts at: 493 1204 3498 493
Size: 711 2294 2702
Fragments arranged by size:
2702 2294 711
BsaBI GATnn'nnATC
Cuts at: 5701 5701
Size: 5707
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3514 3896 4531 275
Size: 53 83 186 936 1981 382 635 1451
Fragments arranged by size:
1981 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2205 4140 4538 4812 4959 5370 2205
Size: 1935 398 274 147 411 2542
Fragments arranged by size:
2542 1935 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2183 2213 1178
Size: 30 165 30 780 30 4672
Fragments arranged by size:
4672 780 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2039 2042 2045 2048 2051 2276 2288 1186
Size: 853 3 3 3 3 225 12 4605
Fragments arranged by size:
4605 853 225 12 3 3 3 3
BseYI C'CCAG_C
Cuts at: 4910 5406 4910
Size: 496 5211
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2009 2402 2766 1163
Size: 573 273 393 364 4104
Fragments arranged by size:
4104 573 393 364 273
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3157 3918 4946 1540
Size: 275 186 1156 761 1028 2301
Fragments arranged by size:
2301 1156 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2077 2991 3768 3853 4924 2077
Size: 914 777 85 1071 2860
Fragments arranged by size:
2860 1071 914 777 85
BsmI GAATG_Cn'
Cuts at: 3218 3311 3218
Size: 93 5614
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2077 2109 2991 3026 3768 3853 4924
Size: 399 287 32 882 35 742 85 1071
Cuts at: 4924 1391
Size: 2174
Fragments arranged by size:
2174 1071 882 742 399 287 85 35
32
BspEI T'CCGG_A
Cuts at: 5370 5370
Size: 5707
BspHI T'CATG_A
Cuts at: 3596 5326 3596
Size: 1730 3977
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2385 3062 844
Size: 1541 677 3489
Fragments arranged by size:
3489 1541 677
BsrBI CCG'CTC
Cuts at: 1394 1823 3594 1394
Size: 429 1771 3507
Fragments arranged by size:
3507 1771 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2787 4202 4376 65
Size: 1183 1539 1415 174 1396
Fragments arranged by size:
1539 1415 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4351 4538 1700
Size: 110 2541 187 2869
Fragments arranged by size:
2869 2541 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5707
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5707
BssSI C'ACGA_G
Cuts at: 3767 4779 3767
Size: 1012 4695
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5707
BstBI TT'CG_AA
Cuts at: 4511 4511
Size: 5707
BstEII G'GTnAC_C
Cuts at: 1184 2611 1184
Size: 1427 4280
BstYI r'GATC_y
Cuts at: 1972 3037 3404 3789 3806 5258 5344 5356 5702
Size: 1065 367 385 17 1452 86 12 346
Cuts at: 5702 1972
Size: 1977
Fragments arranged by size:
1977 1452 1065 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2127 2616 513
Size: 552 939 123 489 3604
Fragments arranged by size:
3604 939 552 489 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2974 487
Size: 683 56 1748 3220
Fragments arranged by size:
3220 1748 683 56
BtsI GCAGTG_nn'
Cuts at: 2232 2754 3223 4017 4037 2232
Size: 522 469 794 20 3902
Fragments arranged by size:
3902 794 522 469 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5707
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5672
DraI TTT'AAA
Cuts at: 3011 3011
Size: 5707
DraIII CAC_nnn'GTG
Cuts at: 2535 2535
Size: 5707
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4714 809
Size: 507 3398 1802
Fragments arranged by size:
3398 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2036 2124 2683
Size: 54 1425 50 275 18 206 88 559
Cuts at: 2683 3154 4043 5672 8
Size: 471 889 1629 43
Fragments arranged by size:
1629 1425 889 559 471 275 206 88
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3154 1537
Size: 275 1342 4090
Fragments arranged by size:
4090 1342 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2609 3637 1353
Size: 1256 1028 3423
Fragments arranged by size:
3423 1256 1028
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4294 4668 4814 387
Size: 1064 138 2705 374 146 1280
Fragments arranged by size:
2705 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2989 2989
Size: 5707
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2513 2915 3770 4367 5154 5422 1239
Size: 393 881 402 855 597 787 268 1524
Fragments arranged by size:
1524 881 855 787 597 402 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2573 2573
Size: 5707
EcoO109I rG'GnC_Cy
Cuts at: 2105 2397 2542 2105
Size: 292 145 5270
Fragments arranged by size:
5270 292 145
EcoRI G'AATT_C
Cuts at: 3016 3394 3016
Size: 378 5329
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2960 2960
Size: 5707
FspI TGC'GCA
Cuts at: 4213 4213
Size: 5707
HaeII r_GCGC'y
Cuts at: 1678 4854 1678
Size: 3176 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2486 2518 4348 4380 4422
Size: 32 135 32 914 32 1830 32 42
Cuts at: 4422 4454 1373
Size: 32 2626
Fragments arranged by size:
2626 1830 914 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3051 3297 669
Size: 988 281 1113 246 3079
Fragments arranged by size:
3079 1113 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5707
HpaI GTT'AAC
Cuts at: 3297 3297
Size: 5707
KpnI G_GTAC'C
Cuts at: 2518 3032 2518
Size: 514 5193
MfeI C'AATT_G
Cuts at: 3306 3306
Size: 5707
MluI A'CGCG_T
Cuts at: 5583 5583
Size: 5707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2113 4821 5005 1305
Size: 808 2708 184 2007
Fragments arranged by size:
2708 2007 808 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2126 10
Size: 54 1425 343 294 3591
Fragments arranged by size:
3591 1425 343 294 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2192 2297 2459 2621 3665 4024 4183
Size: 648 1026 105 162 162 1044 359 159
Cuts at: 4183 518
Size: 2042
Fragments arranged by size:
2042 1044 1026 648 359 162 162 159
105
MspA1I CmG'CkG
Cuts at: 1606 2015 2376 3123 3800 4948 5193 1606
Size: 409 361 747 677 1148 245 2120
Fragments arranged by size:
2120 1148 747 677 409 361 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4216
Fragments arranged by size:
4216 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5707
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5707
NotI GC'GGCC_GC
Cuts at: 3154 3154
Size: 5707
NsiI A_TGCA'T
Cuts at: 2801 2801
Size: 5707
NspI r_CATG'y
Cuts at: 1132 1229 2656 2984 3065 4610 1132
Size: 97 1427 328 81 1545 2229
Fragments arranged by size:
2229 1545 1427 328 97 81
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5707
PciI A'CATG_T
Cuts at: 2652 4606 2652
Size: 1954 3753
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5707
PmeI GTTT'AAAC
Cuts at: 3011 3011
Size: 5707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3776 3808 5289 5321 3776
Size: 32 1481 32 4162
Fragments arranged by size:
4162 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2397 2542 2397
Size: 145 5562
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5707
PsiI TTA'TAA
Cuts at: 3277 3277
Size: 5707
PspOMI G'GGCC_C
Cuts at: 2105 2105
Size: 5707
PstI C_TGCA'G
Cuts at: 830 3059 830
Size: 2229 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5707
PvuII CAG'CTG
Cuts at: 2015 2015
Size: 5707
SacI G_AGCT'C
Cuts at: 2991 2991
Size: 5707
SalI G'TCGA_C
Cuts at: 1936 3049 1936
Size: 1113 4594
SanDI GG'GwC_CC
Cuts at: 2542 2542
Size: 5707
SbfI CC_TGCA'GG
Cuts at: 3059 3059
Size: 5707
ScaI AGT'ACT
Cuts at: 3955 5667 3955
Size: 1712 3995
SfcI C'TryA_G
Cuts at: 826 1045 3055 4190 4871 5062 826
Size: 219 2010 1135 681 191 1471
Fragments arranged by size:
2010 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5707
SmaI CCC'GGG
Cuts at: 1872 3034 1872
Size: 1162 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3811 4712 4974 820
Size: 197 926 1868 901 262 1553
Fragments arranged by size:
1868 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3498 493
Size: 3005 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5707
SphI G_CATG'C
Cuts at: 1132 3065 1132
Size: 1933 3774
SspI AAT'ATT
Cuts at: 5 52 3631 5
Size: 47 3579 2081
Fragments arranged by size:
3579 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5707
StyI C'CwwG_G
Cuts at: 513 1065 2004 3146 513
Size: 552 939 1142 3074
Fragments arranged by size:
3074 1142 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3734 3751 3904 4089 4534 325
Size: 1255 2154 17 153 185 445 1498
Fragments arranged by size:
2154 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3953 5665 96
Size: 274 80 33 51 1443 1976 1712 138
Fragments arranged by size:
1976 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2183 2215 5667 5699 2183
Size: 32 3452 32 2191
Fragments arranged by size:
3452 2191 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5707
XbaI T'CTAG_A
Cuts at: 2504 3043 2504
Size: 539 5168
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5707
XmaI C'CCGG_G
Cuts at: 1870 3032 1870
Size: 1162 4545
XmnI GAAnn'nnTTC
Cuts at: 2925 3836 2925
Size: 911 4796
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3515 276
Size: 53 83 186 936 1981 2468
Fragments arranged by size:
2468 1981 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI
SrfI SwaI XcmI