Restriction map for
pFN21AE6605
(Circular) (Six-base) MAPSORT of: pFN21AE6605.seq Check: 2619 from: 1 to: 5518
pFN21AE6605 5518 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3328 278
Size: 53 83 186 936 1792 2468
Fragments arranged by size:
2468 1792 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2861 1937
Size: 924 4594
Acc65I G'GTAC_C
Cuts at: 2839 2839
Size: 5518
AclI AA'CG_TT
Cuts at: 1563 3645 4018 1563
Size: 2082 373 3063
Fragments arranged by size:
3063 2082 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3581 4965 5233 1632
Size: 1949 1384 268 1917
Fragments arranged by size:
1949 1917 1384 268
AfeI AGC'GCT
Cuts at: 2489 2489
Size: 5518
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5321
AflIII A'CryG_T
Cuts at: 1203 4417 5394 1203
Size: 3214 977 1327
Fragments arranged by size:
3214 1327 977
AgeI A'CCGG_T
Cuts at: 4349 4349
Size: 5518
AhdI GACnn_n'nnGTC
Cuts at: 4247 4247
Size: 5518
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5518
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2372 1184
Size: 1188 4330
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2319 2351 2319
Size: 32 5486
AlwNI CAG_nnn'CTG
Cuts at: 3261 4833 3261
Size: 1572 3946
ApaLI G'TGCA_C
Cuts at: 3575 4731 3575
Size: 1156 4362
ApoI r'AATT_y
Cuts at: 1493 2827 3053 3205 5314 1493
Size: 1334 226 152 2109 1697
Fragments arranged by size:
2109 1697 1334 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4072 160
Size: 872 3040 1606
Fragments arranged by size:
3040 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5518
AvaI C'yCGr_G
Cuts at: 1870 1943 2746 2843 1870
Size: 73 803 97 4545
Fragments arranged by size:
4545 803 97 73
BamHI G'GATC_C
Cuts at: 2848 3215 2848
Size: 367 5151
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2162 2639 2839 4294 618
Size: 325 217 627 375 477 200 1455 1842
Fragments arranged by size:
1842 1455 627 477 375 325 217 200
BanII G_rGCy'C
Cuts at: 1391 2412 2703 2837 1391
Size: 1021 291 134 4072
Fragments arranged by size:
4072 1021 291 134
BbeI G_GCGC'C
Cuts at: 2166 2643 2166
Size: 477 5041
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2599 928
Size: 971 700 3847
Fragments arranged by size:
3847 971 700
BbvCI CC'TCA_GC
Cuts at: 2079 2079
Size: 5518
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2350 2384 3709 3743 1903
Size: 34 413 34 1325 34 3678
Fragments arranged by size:
3678 1325 413 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3410 4620 3410
Size: 1210 4308
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5518
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2526 2597 4129
Size: 107 122 71 631 605 854 71 1532
Cuts at: 4129 136
Size: 1525
Fragments arranged by size:
1532 1525 854 631 605 122 107 71
71
BglII A'GATC_T
Cuts at: 2460 5513 2460
Size: 3053 2465
BlpI GC'TnA_GC
Cuts at: 2534 2935 2534
Size: 401 5117
Bme1580I G_kGCm'C
Cuts at: 1790 2531 3579 4735 1790
Size: 741 1048 1156 2573
Fragments arranged by size:
2573 1156 1048 741
BmgBI CAC'GTC
Cuts at: 1318 2764 1318
Size: 1446 4072
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3249 4207 442
Size: 416 53 83 399 24 1832 958 1753
Fragments arranged by size:
1832 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2648 4178 1239
Size: 1409 1530 2579
Fragments arranged by size:
2579 1530 1409
Bpu10I CC'TnA_GC
Cuts at: 2079 2079
Size: 5518
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2396 3643 4508 4806 2396
Size: 1247 865 298 3108
Fragments arranged by size:
3108 1247 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2395 4181 882
Size: 617 896 1786 2219
Fragments arranged by size:
2219 1786 896 617
BsaAI yAC'GTr
Cuts at: 493 1204 3309 493
Size: 711 2105 2702
Fragments arranged by size:
2702 2105 711
BsaBI GATnn'nnATC
Cuts at: 5512 5512
Size: 5518
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2097 2163 2499 2640
Size: 53 83 186 936 564 66 336 141
Cuts at: 2640 3325 3707 4342 275
Size: 685 382 635 1451
Fragments arranged by size:
1451 936 685 635 564 382 336 186
141 83 66 53
BsaWI w'CCGG_w
Cuts at: 2294 3951 4349 4623 4770 5181 2294
Size: 1657 398 274 147 411 2631
Fragments arranged by size:
2631 1657 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2319 2349 1178
Size: 30 165 30 916 30 4347
Fragments arranged by size:
4347 916 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2495 2735 1186
Size: 1309 240 3969
Fragments arranged by size:
3969 1309 240
BseYI C'CCAG_C
Cuts at: 2502 4721 5217 2502
Size: 2219 496 2803
Fragments arranged by size:
2803 2219 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2129 2368 1163
Size: 573 393 239 4313
Fragments arranged by size:
4313 573 393 239
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2968 3729 4757 1540
Size: 275 186 967 761 1028 2301
Fragments arranged by size:
2301 1028 967 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2412 3579 3664 4735 2412
Size: 1167 85 1071 3195
Fragments arranged by size:
3195 1167 1071 85
BsmI GAATG_Cn'
Cuts at: 2431 3029 3122 2431
Size: 598 93 4827
Fragments arranged by size:
4827 598 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2441 2441
Size: 5518
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2412 2531 2703 2837 3579 3664 4735
Size: 399 622 119 172 134 742 85 1071
Cuts at: 4735 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 622 399 172 134 119
85
BspEI T'CCGG_A
Cuts at: 5181 5181
Size: 5518
BspHI T'CATG_A
Cuts at: 3407 5137 3407
Size: 1730 3788
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2475 2873 844
Size: 1631 398 3489
Fragments arranged by size:
3489 1631 398
BsrBI CCG'CTC
Cuts at: 1394 1823 3405 1394
Size: 429 1582 3507
Fragments arranged by size:
3507 1582 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4013 4187 65
Size: 1183 2765 174 1396
Fragments arranged by size:
2765 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2083 2435 2591 4162 4349 1700
Size: 110 273 352 156 1571 187 2869
Fragments arranged by size:
2869 1571 352 273 187 156 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5518
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5518
BssSI C'ACGA_G
Cuts at: 3578 4590 3578
Size: 1012 4506
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5518
BstBI TT'CG_AA
Cuts at: 4322 4322
Size: 5518
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5518
BstYI r'GATC_y
Cuts at: 1972 2112 2460 2848 3215 3600 3617 5069 5155
Size: 140 348 388 367 385 17 1452 86
Cuts at: 5155 5167 5513 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 388 385 367 348 346 140
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2193 2521 513
Size: 552 1128 328 3510
Fragments arranged by size:
3510 1128 552 328
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2106 487
Size: 683 56 880 3899
Fragments arranged by size:
3899 880 683 56
BtsI GCAGTG_nn'
Cuts at: 2057 2242 3034 3828 3848 2057
Size: 185 792 794 20 3727
Fragments arranged by size:
3727 794 792 185 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5518
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5483
DraI TTT'AAA
Cuts at: 2822 2822
Size: 5518
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2313 2387 4525 809
Size: 507 997 74 2138 1802
Fragments arranged by size:
2138 1802 997 507 74
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2571 2965 3854
Size: 54 1425 50 275 18 741 394 889
Cuts at: 3854 5483 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 741 394 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2965 1537
Size: 275 1153 4090
Fragments arranged by size:
4090 1153 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2176 3448 1353
Size: 823 1272 3423
Fragments arranged by size:
3423 1272 823
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4105 4479 4625 387
Size: 1064 138 2516 374 146 1280
Fragments arranged by size:
2516 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2410 2410
Size: 5518
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2648 3581 4178 4965 5233 1239
Size: 393 1016 933 597 787 268 1524
Fragments arranged by size:
1524 1016 933 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2630 2630
Size: 5518
EcoO109I rG'GnC_Cy
Cuts at: 2306 2306
Size: 5518
EcoRI G'AATT_C
Cuts at: 2827 3205 2827
Size: 378 5140
FspI TGC'GCA
Cuts at: 2360 4024 2360
Size: 1664 3854
HaeII r_GCGC'y
Cuts at: 1678 2166 2491 2643 4665 1678
Size: 488 325 152 2022 2531
Fragments arranged by size:
2531 2022 488 325 152
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 1999 2031 2087 2119 4159
Size: 32 135 32 427 32 56 32 2040
Cuts at: 4159 4191 4233 4265 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2040 427 135 56 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2862 3108 669
Size: 988 281 924 246 3079
Fragments arranged by size:
3079 988 924 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5518
HpaI GTT'AAC
Cuts at: 3108 3108
Size: 5518
KasI G'GCGC_C
Cuts at: 2162 2639 2162
Size: 477 5041
KpnI G_GTAC'C
Cuts at: 2843 2843
Size: 5518
MfeI C'AATT_G
Cuts at: 3117 3117
Size: 5518
MluI A'CGCG_T
Cuts at: 5394 5394
Size: 5518
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4632 4816 1305
Size: 3327 184 2007
Fragments arranged by size:
3327 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3696
Fragments arranged by size:
3696 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2344 2780 3476 3835 3994 518
Size: 648 1178 436 696 359 159 2042
Fragments arranged by size:
2042 1178 696 648 436 359 159
MspA1I CmG'CkG
Cuts at: 1606 2356 2398 2506 2523 2694 2934 3611 4759
Size: 750 42 108 17 171 240 677 1148
Cuts at: 4759 5004 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 750 677 245 240 171 108
42 17
NaeI GCC'GGC
Cuts at: 2085 2593 2085
Size: 508 5010
NarI GG'CG_CC
Cuts at: 2163 2640 2163
Size: 477 5041
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4966
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5518
NgoMIV G'CCGG_C
Cuts at: 2083 2591 2083
Size: 508 5010
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5518
NotI GC'GGCC_GC
Cuts at: 2965 2965
Size: 5518
NspI r_CATG'y
Cuts at: 1132 1229 2542 2876 4421 1132
Size: 97 1313 334 1545 2229
Fragments arranged by size:
2229 1545 1313 334 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5518
PciI A'CATG_T
Cuts at: 4417 4417
Size: 5518
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5518
PmeI GTTT'AAAC
Cuts at: 2822 2822
Size: 5518
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2319 2351 2399 2431 3587 3619 5100 5132 2319
Size: 32 48 32 1156 32 1481 32 2705
Fragments arranged by size:
2705 1481 1156 48 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2306 2306
Size: 5518
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5518
PsiI TTA'TAA
Cuts at: 3088 3088
Size: 5518
PstI C_TGCA'G
Cuts at: 830 2870 830
Size: 2040 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5518
PvuII CAG'CTG
Cuts at: 2356 2398 2506 2356
Size: 42 108 5368
Fragments arranged by size:
5368 108 42
SacI G_AGCT'C
Cuts at: 2412 2412
Size: 5518
SacII CC_GC'GG
Cuts at: 2524 2524
Size: 5518
SalI G'TCGA_C
Cuts at: 1936 2860 1936
Size: 924 4594
SbfI CC_TGCA'GG
Cuts at: 2870 2870
Size: 5518
ScaI AGT'ACT
Cuts at: 3766 5478 3766
Size: 1712 3806
SexAI A'CCwGG_T
Cuts at: 2385 2385
Size: 5518
SfcI C'TryA_G
Cuts at: 826 1045 2866 4001 4682 4873 826
Size: 219 1821 1135 681 191 1471
Fragments arranged by size:
1821 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2164 2641 2164
Size: 477 5041
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5518
SgrAI Cr'CCGG_yG
Cuts at: 1700 2435 1700
Size: 735 4783
SmaI CCC'GGG
Cuts at: 1872 2845 1872
Size: 973 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2411 3622 4523 4785 820
Size: 197 926 468 1211 901 262 1553
Fragments arranged by size:
1553 1211 926 901 468 262 197
SnaBI TAC'GTA
Cuts at: 493 3309 493
Size: 2816 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5518
SphI G_CATG'C
Cuts at: 1132 2542 2876 1132
Size: 1410 334 3774
Fragments arranged by size:
3774 1410 334
SspI AAT'ATT
Cuts at: 5 52 3442 5
Size: 47 3390 2081
Fragments arranged by size:
3390 2081 47
StuI AGG'CCT
Cuts at: 1517 2334 1517
Size: 817 4701
StyI C'CwwG_G
Cuts at: 513 1065 2544 2957 513
Size: 552 1479 413 3074
Fragments arranged by size:
3074 1479 552 413
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3545 3562 3715 3900 4345 325
Size: 1255 1965 17 153 185 445 1498
Fragments arranged by size:
1965 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2649 2784 3764
Size: 274 80 33 51 1443 672 135 980
Cuts at: 3764 5476 96
Size: 1712 138
Fragments arranged by size:
1712 1443 980 672 274 138 135 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5478 5510 5478
Size: 32 5486
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5518
XbaI T'CTAG_A
Cuts at: 2854 2854
Size: 5518
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2374 2566 2374
Size: 192 5326
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5518
XmaI C'CCGG_G
Cuts at: 1870 2843 1870
Size: 973 4545
XmnI GAAnn'nnTTC
Cuts at: 3647 3647
Size: 5518
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3326 276
Size: 53 83 186 936 1792 2468
Fragments arranged by size:
2468 1792 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BfrBI BplI BsiWI
BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SanDI SapI SfiI SrfI SwaI