Restriction map for
pFN21AE6604
(Circular) (Six-base) MAPSORT of: pFN21AE6604.seq Check: 1413 from: 1 to: 5434
pFN21AE6604 5434 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2173 2173
Size: 5434
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3244 278
Size: 53 83 186 936 1708 2468
Fragments arranged by size:
2468 1708 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2777 1937
Size: 840 4594
Acc65I G'GTAC_C
Cuts at: 2755 2755
Size: 5434
AclI AA'CG_TT
Cuts at: 1563 3561 3934 1563
Size: 1998 373 3063
Fragments arranged by size:
3063 1998 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3497 4881 5149 1632
Size: 1865 1384 268 1917
Fragments arranged by size:
1917 1865 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5237
AflIII A'CryG_T
Cuts at: 1203 4333 5310 1203
Size: 3130 977 1327
Fragments arranged by size:
3130 1327 977
AgeI A'CCGG_T
Cuts at: 2017 4265 2017
Size: 2248 3186
AhdI GACnn_n'nnGTC
Cuts at: 4163 4163
Size: 5434
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5434
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2251 1184
Size: 1067 4367
AlwNI CAG_nnn'CTG
Cuts at: 3177 4749 3177
Size: 1572 3862
ApaLI G'TGCA_C
Cuts at: 2236 3491 4647 2236
Size: 1255 1156 3023
Fragments arranged by size:
3023 1255 1156
ApoI r'AATT_y
Cuts at: 1493 2727 2743 2969 3121 5230 1493
Size: 1234 16 226 152 2109 1697
Fragments arranged by size:
2109 1697 1234 226 152 16
AseI AT'TA_AT
Cuts at: 160 1032 3988 160
Size: 872 2956 1606
Fragments arranged by size:
2956 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5434
AvaI C'yCGr_G
Cuts at: 1870 1943 2759 1870
Size: 73 816 4545
Fragments arranged by size:
4545 816 73
BamHI G'GATC_C
Cuts at: 2071 2764 3131 2071
Size: 693 367 4374
Fragments arranged by size:
4374 693 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2755 4210 618
Size: 325 217 627 968 1455 1842
Fragments arranged by size:
1842 1455 968 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2753 1391
Size: 1362 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2319 928
Size: 971 420 4043
Fragments arranged by size:
4043 971 420
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3625 3659 1903
Size: 34 1688 34 3678
Fragments arranged by size:
3678 1688 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3326 4536 3326
Size: 1210 4224
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5434
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4045 136
Size: 107 122 71 631 605 2373 1525
Fragments arranged by size:
2373 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2629 5429 2629
Size: 2800 2634
BlpI GC'TnA_GC
Cuts at: 2851 2851
Size: 5434
Bme1580I G_kGCm'C
Cuts at: 1790 2240 3495 4651 1790
Size: 450 1255 1156 2573
Fragments arranged by size:
2573 1255 1156 450
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5434
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2095 3165 4123
Size: 416 53 83 399 24 678 1070 958
Cuts at: 4123 442
Size: 1753
Fragments arranged by size:
1753 1070 958 678 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5434
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4094 1239
Size: 2855 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2672 3559 4424 4722 2672
Size: 887 865 298 3384
Fragments arranged by size:
3384 887 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4097 882
Size: 617 2598 2219
Fragments arranged by size:
2598 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3225 493
Size: 711 2021 2702
Fragments arranged by size:
2702 2021 711
BsaBI GATnn'nnATC
Cuts at: 5428 5428
Size: 5434
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3241 3623 4258 275
Size: 53 83 186 936 1708 382 635 1451
Fragments arranged by size:
1708 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2017 3867 4265 4539 4686 5097 2017
Size: 1850 398 274 147 411 2354
Fragments arranged by size:
2354 1850 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5209
Fragments arranged by size:
5209 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5434
BseYI C'CCAG_C
Cuts at: 4637 5133 4637
Size: 496 4938
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4861
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2884 3645 4673 1540
Size: 275 186 883 761 1028 2301
Fragments arranged by size:
2301 1028 883 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2240 3495 3580 4651 2240
Size: 1255 85 1071 3023
Fragments arranged by size:
3023 1255 1071 85
BsmI GAATG_Cn'
Cuts at: 2218 2945 3038 2218
Size: 727 93 4614
Fragments arranged by size:
4614 727 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2240 2753 3495 3580 4651 1391
Size: 399 450 513 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 513 450 399 85
BspEI T'CCGG_A
Cuts at: 5097 5097
Size: 5434
BspHI T'CATG_A
Cuts at: 3323 5053 3323
Size: 1730 3704
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2065 2173 2789 844
Size: 1221 108 616 3489
Fragments arranged by size:
3489 1221 616 108
BsrBI CCG'CTC
Cuts at: 1394 1823 3321 1394
Size: 429 1498 3507
Fragments arranged by size:
3507 1498 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3929 4103 65
Size: 1183 2681 174 1396
Fragments arranged by size:
2681 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2017 2573 4078 4265 1700
Size: 110 207 556 1505 187 2869
Fragments arranged by size:
2869 1505 556 207 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5434
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5434
BssSI C'ACGA_G
Cuts at: 3494 4506 3494
Size: 1012 4422
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5434
BstBI TT'CG_AA
Cuts at: 4238 4238
Size: 5434
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5434
BstYI r'GATC_y
Cuts at: 1972 2071 2355 2629 2764 3131 3516 3533 4985
Size: 99 284 274 135 367 385 17 1452
Cuts at: 4985 5071 5083 5429 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 284 274 135
99 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3943
Fragments arranged by size:
3943 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4695
Fragments arranged by size:
4695 683 56
BtsI GCAGTG_nn'
Cuts at: 2950 3744 3764 2950
Size: 794 20 4620
Fragments arranged by size:
4620 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5434
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5399
DraI TTT'AAA
Cuts at: 2738 2738
Size: 5434
DraIII CAC_nnn'GTG
Cuts at: 2570 2570
Size: 5434
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2388 4441 809
Size: 507 1072 2053 1802
Fragments arranged by size:
2053 1802 1072 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2881 3770 5399
Size: 54 1425 50 275 18 1051 889 1629
Cuts at: 5399 8
Size: 43
Fragments arranged by size:
1629 1425 1051 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2881 1537
Size: 275 1069 4090
Fragments arranged by size:
4090 1069 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3364 1353
Size: 2011 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4021 4395 4541 387
Size: 1064 138 2432 374 146 1280
Fragments arranged by size:
2432 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3497 4094 4881 5149 1239
Size: 393 1865 597 787 268 1524
Fragments arranged by size:
1865 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2061 2061
Size: 5434
EcoRI G'AATT_C
Cuts at: 2743 3121 2743
Size: 378 5056
FspI TGC'GCA
Cuts at: 3940 3940
Size: 5434
HaeII r_GCGC'y
Cuts at: 1678 4581 1678
Size: 2903 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4075 4107 4149 4181 1373
Size: 32 135 32 2503 32 42 32 2626
Fragments arranged by size:
2626 2503 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2094 2778 3024 669
Size: 988 281 156 684 246 3079
Fragments arranged by size:
3079 988 684 281 246 156
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5434
HpaI GTT'AAC
Cuts at: 2094 3024 2094
Size: 930 4504
KpnI G_GTAC'C
Cuts at: 2759 2759
Size: 5434
MfeI C'AATT_G
Cuts at: 3033 3033
Size: 5434
MluI A'CGCG_T
Cuts at: 5310 5310
Size: 5434
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2386 4548 4732 1305
Size: 1081 2162 184 2007
Fragments arranged by size:
2162 2007 1081 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3612
Fragments arranged by size:
3612 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3392 3751 3910 518
Size: 648 2226 359 159 2042
Fragments arranged by size:
2226 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2666 2850 3527 4675 4920 1606
Size: 1060 184 677 1148 245 2120
Fragments arranged by size:
2120 1148 1060 677 245 184
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3943
Fragments arranged by size:
3943 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5434
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5434
NotI GC'GGCC_GC
Cuts at: 2881 2881
Size: 5434
NspI r_CATG'y
Cuts at: 1132 1229 2179 2792 4337 1132
Size: 97 950 613 1545 2229
Fragments arranged by size:
2229 1545 950 613 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5434
PciI A'CATG_T
Cuts at: 4333 4333
Size: 5434
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5434
PmeI GTTT'AAAC
Cuts at: 2738 2738
Size: 5434
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3503 3535 5016 5048 3503
Size: 32 1481 32 3889
Fragments arranged by size:
3889 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5434
PsiI TTA'TAA
Cuts at: 3004 3004
Size: 5434
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2667 2699 2714 2746 2667
Size: 32 15 32 5355
Fragments arranged by size:
5355 32 32 15
PstI C_TGCA'G
Cuts at: 830 2062 2786 830
Size: 1232 724 3478
Fragments arranged by size:
3478 1232 724
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5434
PvuII CAG'CTG
Cuts at: 2666 2666
Size: 5434
SalI G'TCGA_C
Cuts at: 1936 2776 1936
Size: 840 4594
SbfI CC_TGCA'GG
Cuts at: 2786 2786
Size: 5434
ScaI AGT'ACT
Cuts at: 3682 5394 3682
Size: 1712 3722
SexAI A'CCwGG_T
Cuts at: 2442 2442
Size: 5434
SfcI C'TryA_G
Cuts at: 826 1045 2058 2782 3917 4598 4789 826
Size: 219 1013 724 1135 681 191 1471
Fragments arranged by size:
1471 1135 1013 724 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5434
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5434
SmaI CCC'GGG
Cuts at: 1872 2761 1872
Size: 889 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2687 3538 4439 4701 820
Size: 197 926 744 851 901 262 1553
Fragments arranged by size:
1553 926 901 851 744 262 197
SnaBI TAC'GTA
Cuts at: 493 3225 493
Size: 2732 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5434
SphI G_CATG'C
Cuts at: 1132 2792 1132
Size: 1660 3774
SspI AAT'ATT
Cuts at: 5 52 2510 3358 5
Size: 47 2458 848 2081
Fragments arranged by size:
2458 2081 848 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5434
StyI C'CwwG_G
Cuts at: 513 1065 2004 2873 513
Size: 552 939 869 3074
Fragments arranged by size:
3074 939 869 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2114 3461 3478 3631 3816 4261 325
Size: 1255 534 1347 17 153 185 445 1498
Fragments arranged by size:
1498 1347 1255 534 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2276 3680 5392
Size: 274 80 33 51 1443 299 1404 1712
Cuts at: 5392 96
Size: 138
Fragments arranged by size:
1712 1443 1404 299 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5394 5426 5394
Size: 32 5402
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5434
XbaI T'CTAG_A
Cuts at: 2770 2770
Size: 5434
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2450 2450
Size: 5434
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5434
XmaI C'CCGG_G
Cuts at: 1870 2759 1870
Size: 889 4545
XmnI GAAnn'nnTTC
Cuts at: 3563 3563
Size: 5434
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3242 276
Size: 53 83 186 936 1708 2468
Fragments arranged by size:
2468 1708 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI RsrII SacI SacII SanDI SapI SfiI
SfoI SrfI SwaI