Restriction map for
pFN21AE6602
(Circular) (Six-base) MAPSORT of: pFN21AE6602.seq Check: 9860 from: 1 to: 5419
pFN21AE6602 5419 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3229 278
Size: 53 83 186 936 1693 2468
Fragments arranged by size:
2468 1693 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2762 1937
Size: 825 4594
Acc65I G'GTAC_C
Cuts at: 2740 2740
Size: 5419
AclI AA'CG_TT
Cuts at: 1563 2435 3546 3919 1563
Size: 872 1111 373 3063
Fragments arranged by size:
3063 1111 872 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2566 2726 3482 4866 5134 1632
Size: 934 160 756 1384 268 1917
Fragments arranged by size:
1917 1384 934 756 268 160
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5222
AflIII A'CryG_T
Cuts at: 1203 4318 5295 1203
Size: 3115 977 1327
Fragments arranged by size:
3115 1327 977
AgeI A'CCGG_T
Cuts at: 4250 4250
Size: 5419
AhdI GACnn_n'nnGTC
Cuts at: 4148 4148
Size: 5419
AleI CACnn'nnGTG
Cuts at: 1166 2535 1166
Size: 1369 4050
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5419
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2306 2338 2306
Size: 32 5387
AlwNI CAG_nnn'CTG
Cuts at: 2095 2098 3162 4734 2095
Size: 3 1064 1572 2780
Fragments arranged by size:
2780 1572 1064 3
ApaI G_GGCC'C
Cuts at: 2131 2578 2131
Size: 447 4972
ApaLI G'TGCA_C
Cuts at: 3476 4632 3476
Size: 1156 4263
ApoI r'AATT_y
Cuts at: 1493 2728 2954 3106 5215 1493
Size: 1235 226 152 2109 1697
Fragments arranged by size:
2109 1697 1235 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3973 160
Size: 872 2941 1606
Fragments arranged by size:
2941 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5419
AvaI C'yCGr_G
Cuts at: 1870 1943 2744 1870
Size: 73 801 4545
Fragments arranged by size:
4545 801 73
BamHI G'GATC_C
Cuts at: 2749 3116 2749
Size: 367 5052
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2379 2615 2740 4195 618
Size: 325 217 627 592 236 125 1455 1842
Fragments arranged by size:
1842 1455 627 592 325 236 217 125
BanII G_rGCy'C
Cuts at: 1391 2109 2131 2578 2738 1391
Size: 718 22 447 160 4072
Fragments arranged by size:
4072 718 447 160 22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4448
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3610 3644 1903
Size: 34 1673 34 3678
Fragments arranged by size:
3678 1673 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2308 3311 4521 2308
Size: 1003 1210 3206
Fragments arranged by size:
3206 1210 1003
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5419
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2126 4030 136
Size: 107 122 71 631 605 454 1904 1525
Fragments arranged by size:
1904 1525 631 605 454 122 107 71
BglII A'GATC_T
Cuts at: 5414 5414
Size: 5419
BlpI GC'TnA_GC
Cuts at: 2836 2836
Size: 5419
Bme1580I G_kGCm'C
Cuts at: 1790 2131 2578 3480 4636 1790
Size: 341 447 902 1156 2573
Fragments arranged by size:
2573 1156 902 447 341
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5419
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3150 4108 442
Size: 416 53 83 399 24 1733 958 1753
Fragments arranged by size:
1753 1733 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5419
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2038 2279 2318 4079 1239
Size: 799 241 39 1761 2579
Fragments arranged by size:
2579 1761 799 241 39
Bpu10I CC'TnA_GC
Cuts at: 2027 2027
Size: 5419
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2192 2213 3544 4409 4707 2192
Size: 21 1331 865 298 2904
Fragments arranged by size:
2904 1331 865 298 21
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4082 882
Size: 617 2583 2219
Fragments arranged by size:
2583 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2605 3210 493
Size: 711 1401 605 2702
Fragments arranged by size:
2702 1401 711 605
BsaBI GATnn'nnATC
Cuts at: 5413 5413
Size: 5419
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3226 3608 4243 275
Size: 53 83 186 936 1693 382 635 1451
Fragments arranged by size:
1693 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3852 4250 4524 4671 5082 3852
Size: 398 274 147 411 4189
Fragments arranged by size:
4189 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5194
Fragments arranged by size:
5194 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2192 2195 2630 1186
Size: 1006 3 435 3975
Fragments arranged by size:
3975 1006 435 3
BseYI C'CCAG_C
Cuts at: 2088 2108 2571 4622 5118 2088
Size: 20 463 2051 496 2389
Fragments arranged by size:
2389 2051 496 463 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2513 1163
Size: 573 777 4069
Fragments arranged by size:
4069 777 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2869 3630 4658 1540
Size: 275 186 868 761 1028 2301
Fragments arranged by size:
2301 1028 868 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3480 3565 4636 3480
Size: 85 1071 4263
Fragments arranged by size:
4263 1071 85
BsmI GAATG_Cn'
Cuts at: 2930 3023 2930
Size: 93 5326
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2109 2131 2578 2738 3480 3565 4636
Size: 399 319 22 447 160 742 85 1071
Cuts at: 4636 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 447 399 319 160 85
22
BspEI T'CCGG_A
Cuts at: 5082 5082
Size: 5419
BspHI T'CATG_A
Cuts at: 2553 3308 5038 2553
Size: 755 1730 2934
Fragments arranged by size:
2934 1730 755
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2637 2707 2774 844
Size: 1793 70 67 3489
Fragments arranged by size:
3489 1793 70 67
BsrBI CCG'CTC
Cuts at: 1394 1823 3306 1394
Size: 429 1483 3507
Fragments arranged by size:
3507 1483 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3914 4088 65
Size: 1183 2666 174 1396
Fragments arranged by size:
2666 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4063 4250 1700
Size: 110 2253 187 2869
Fragments arranged by size:
2869 2253 187 110
BsrGI T'GTAC_A
Cuts at: 96 2445 96
Size: 2349 3070
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5419
BssSI C'ACGA_G
Cuts at: 3479 4491 3479
Size: 1012 4407
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5419
BstBI TT'CG_AA
Cuts at: 4223 4223
Size: 5419
BstEII G'GTnAC_C
Cuts at: 1184 2083 1184
Size: 899 4520
BstYI r'GATC_y
Cuts at: 1972 2749 3116 3501 3518 4970 5056 5068 5414
Size: 777 367 385 17 1452 86 12 346
Cuts at: 5414 1972
Size: 1977
Fragments arranged by size:
1977 1452 777 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3928
Fragments arranged by size:
3928 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4680
Fragments arranged by size:
4680 683 56
BtsI GCAGTG_nn'
Cuts at: 2530 2935 3729 3749 2530
Size: 405 794 20 4200
Fragments arranged by size:
4200 794 405 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5419
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5384
DraI TTT'AAA
Cuts at: 2723 2723
Size: 5419
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4426 809
Size: 507 3110 1802
Fragments arranged by size:
3110 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2866 3755 5384
Size: 54 1425 50 275 18 1036 889 1629
Cuts at: 5384 8
Size: 43
Fragments arranged by size:
1629 1425 1036 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2866 1537
Size: 275 1054 4090
Fragments arranged by size:
4090 1054 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2283 2585 3349 1353
Size: 930 302 764 3423
Fragments arranged by size:
3423 930 764 302
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2168 4006 4380 4526 387
Size: 1064 138 579 1838 374 146 1280
Fragments arranged by size:
1838 1280 1064 579 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2038 2279 2318 2566 2726 3482 4079
Size: 393 406 241 39 248 160 756 597
Cuts at: 4079 4866 5134 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 756 597 406 393 268 248
241 160 39
EcoNI CCTnn'n_nnAGG
Cuts at: 2207 2336 2207
Size: 129 5290
EcoO109I rG'GnC_Cy
Cuts at: 2119 2127 2197 2575 2119
Size: 8 70 378 4963
Fragments arranged by size:
4963 378 70 8
EcoRI G'AATT_C
Cuts at: 2728 3106 2728
Size: 378 5041
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2263 2263
Size: 5419
FspI TGC'GCA
Cuts at: 3925 3925
Size: 5419
HaeII r_GCGC'y
Cuts at: 1678 4566 1678
Size: 2888 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2495 2527 4060 4092 4134
Size: 32 135 32 923 32 1533 32 42
Cuts at: 4134 4166 1373
Size: 32 2626
Fragments arranged by size:
2626 1533 923 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2763 3009 669
Size: 988 281 825 246 3079
Fragments arranged by size:
3079 988 825 281 246
HindIII A'AGCT_T
Cuts at: 748 2562 748
Size: 1814 3605
HpaI GTT'AAC
Cuts at: 3009 3009
Size: 5419
KpnI G_GTAC'C
Cuts at: 2744 2744
Size: 5419
MfeI C'AATT_G
Cuts at: 3018 3018
Size: 5419
MluI A'CGCG_T
Cuts at: 5295 5295
Size: 5419
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2082 4533 4717 1305
Size: 777 2451 184 2007
Fragments arranged by size:
2451 2007 777 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3597
Fragments arranged by size:
3597 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2225 2535 3377 3736 3895 518
Size: 648 1059 310 842 359 159 2042
Fragments arranged by size:
2042 1059 842 648 359 310 159
MspA1I CmG'CkG
Cuts at: 1606 2095 2571 2835 3512 4660 4905 1606
Size: 489 476 264 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 489 476 264 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3928
Fragments arranged by size:
3928 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5419
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5419
NotI GC'GGCC_GC
Cuts at: 2866 2866
Size: 5419
NspI r_CATG'y
Cuts at: 1132 1229 2063 2590 2777 4322 1132
Size: 97 834 527 187 1545 2229
Fragments arranged by size:
2229 1545 834 527 187 97
PasI CC'CwG_GG
Cuts at: 1348 2124 1348
Size: 776 4643
PciI A'CATG_T
Cuts at: 4318 4318
Size: 5419
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2305 1108
Size: 1197 4222
PfoI T'CCnGG_A
Cuts at: 2214 2214
Size: 5419
PmeI GTTT'AAAC
Cuts at: 2723 2723
Size: 5419
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3488 3520 5001 5033 3488
Size: 32 1481 32 3874
Fragments arranged by size:
3874 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2197 2197
Size: 5419
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5419
PsiI TTA'TAA
Cuts at: 2989 2989
Size: 5419
PspOMI G'GGCC_C
Cuts at: 2127 2574 2127
Size: 447 4972
PstI C_TGCA'G
Cuts at: 830 2634 2718 2771 830
Size: 1804 84 53 3478
Fragments arranged by size:
3478 1804 84 53
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5419
PvuII CAG'CTG
Cuts at: 2095 2095
Size: 5419
SalI G'TCGA_C
Cuts at: 1936 2761 1936
Size: 825 4594
SbfI CC_TGCA'GG
Cuts at: 2771 2771
Size: 5419
ScaI AGT'ACT
Cuts at: 2294 3667 5379 2294
Size: 1373 1712 2334
Fragments arranged by size:
2334 1712 1373
SfcI C'TryA_G
Cuts at: 826 1045 2277 2630 2714 2767 3902 4583 4774
Size: 219 1232 353 84 53 1135 681 191
Cuts at: 4774 826
Size: 1471
Fragments arranged by size:
1471 1232 1135 681 353 219 191 84
53
SfiI GGCCn_nnn'nGGCC
Cuts at: 2126 2126
Size: 5419
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5419
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5419
SmaI CCC'GGG
Cuts at: 1872 2746 1872
Size: 874 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2192 2207 3523 4424 4686 820
Size: 197 926 249 15 1316 901 262 1553
Fragments arranged by size:
1553 1316 926 901 262 249 197 15
SnaBI TAC'GTA
Cuts at: 493 3210 493
Size: 2717 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5419
SphI G_CATG'C
Cuts at: 1132 2590 2777 1132
Size: 1458 187 3774
Fragments arranged by size:
3774 1458 187
SspI AAT'ATT
Cuts at: 5 52 3343 5
Size: 47 3291 2081
Fragments arranged by size:
3291 2081 47
StuI AGG'CCT
Cuts at: 1517 2681 1517
Size: 1164 4255
StyI C'CwwG_G
Cuts at: 513 1065 2004 2115 2670 2858 513
Size: 552 939 111 555 188 3074
Fragments arranged by size:
3074 939 555 552 188 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2397 3446 3463 3616 3801 4246 325
Size: 1255 817 1049 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1049 817 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2292 2445 3665
Size: 274 80 33 51 1443 315 153 1220
Cuts at: 3665 5377 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1220 315 274 153 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2372 2404 5379 5411 2372
Size: 32 2975 32 2380
Fragments arranged by size:
2975 2380 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5419
XbaI T'CTAG_A
Cuts at: 2755 2755
Size: 5419
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2609 2609
Size: 5419
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5419
XmaI C'CCGG_G
Cuts at: 1870 2744 1870
Size: 874 4545
XmnI GAAnn'nnTTC
Cuts at: 3548 3548
Size: 5419
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3227 276
Size: 53 83 186 936 1693 2468
Fragments arranged by size:
2468 1693 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfoI SrfI SwaI